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DK1499738T3 - Recombinase-polymerease-amplifikation - Google Patents

Recombinase-polymerease-amplifikation

Info

Publication number
DK1499738T3
DK1499738T3 DK03711216T DK03711216T DK1499738T3 DK 1499738 T3 DK1499738 T3 DK 1499738T3 DK 03711216 T DK03711216 T DK 03711216T DK 03711216 T DK03711216 T DK 03711216T DK 1499738 T3 DK1499738 T3 DK 1499738T3
Authority
DK
Denmark
Prior art keywords
dna
amplification
recombinase
polymerease
polymerase
Prior art date
Application number
DK03711216T
Other languages
English (en)
Inventor
Niall A Armes
Derek L Stemple
Original Assignee
Asm Scient Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asm Scient Inc filed Critical Asm Scient Inc
Application granted granted Critical
Publication of DK1499738T3 publication Critical patent/DK1499738T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06FELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90Details of database functions independent of the retrieved data types
    • G06F16/95Retrieval from the web
    • G06F16/951Indexing; Web crawling techniques
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06FELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90Details of database functions independent of the retrieved data types
    • G06F16/95Retrieval from the web
    • G06F16/957Browsing optimisation, e.g. caching or content distillation
    • G06F16/9574Browsing optimisation, e.g. caching or content distillation of access to content, e.g. by caching

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Databases & Information Systems (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Data Mining & Analysis (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
DK03711216T 2002-02-21 2003-02-21 Recombinase-polymerease-amplifikation DK1499738T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35856302P 2002-02-21 2002-02-21
PCT/US2003/005492 WO2003072805A2 (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification

Publications (1)

Publication Number Publication Date
DK1499738T3 true DK1499738T3 (da) 2008-11-17

Family

ID=27765964

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03711216T DK1499738T3 (da) 2002-02-21 2003-02-21 Recombinase-polymerease-amplifikation

Country Status (11)

Country Link
US (7) US7270981B2 (da)
EP (4) EP1499738B1 (da)
JP (4) JP4340543B2 (da)
AT (1) ATE400663T1 (da)
AU (1) AU2003215391B2 (da)
CA (1) CA2476481C (da)
DE (1) DE60322044D1 (da)
DK (1) DK1499738T3 (da)
ES (1) ES2310237T3 (da)
PT (1) PT1499738E (da)
WO (1) WO2003072805A2 (da)

Families Citing this family (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60226856D1 (de) 2001-04-20 2008-07-10 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
AU2003215391B2 (en) * 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
AU2003272438B2 (en) * 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2004092330A2 (en) * 2003-04-11 2004-10-28 Applera Corporation Method of generating long nucleic acid molecules of defined sequence
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
JP5026958B2 (ja) 2004-06-01 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅
AU2011202896B2 (en) * 2004-06-01 2012-12-06 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
AU2005250239A1 (en) 2004-06-02 2005-12-15 Alere San Diego, Inc. 2'-nitrobenzyl-modified ribonucleotides
JP4534138B2 (ja) * 2004-12-21 2010-09-01 アイシン精機株式会社 核酸増幅反応における高度好熱菌由来タンパク質の活性化方法およびその利用
US7579153B2 (en) * 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
CA2616241C (en) 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
WO2007087262A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Selective genome amplification
CA2650993C (en) * 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
EP3978121A1 (en) 2006-07-19 2022-04-06 BioNano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
US8722327B2 (en) 2007-03-28 2014-05-13 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US8628919B2 (en) 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US8198028B2 (en) * 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
JP5846703B2 (ja) 2008-11-18 2016-01-20 バイオナノ ジェノミックス、インク. ポリヌクレオチドのマッピング及び配列決定
EA020593B1 (ru) 2009-01-13 2014-12-30 Флуидигм Корпорейшн Анализ нуклеиновых кислот, полученных из единичных клеток
US8685648B2 (en) 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US20100299773A1 (en) * 2009-05-20 2010-11-25 Monsanto Technology Llc Methods and compositions for selecting an improved plant
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP2438196B1 (en) * 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2010147804A1 (en) * 2009-06-10 2010-12-23 Wisconsin Alumni Research Foundation Method for dna double-strand break repair in vitro and applications thereof
NO20092315A (no) * 2009-06-16 2010-12-06 Agr Cannseal As Brønnverktøy og fremgangsmåte for in situ innføring av et behandlingsfluid i et ringrom i en brønn
US8951733B2 (en) 2009-10-16 2015-02-10 Monsanto Technology Llc Methods of polynucleotide detection
GB0922097D0 (en) 2009-12-17 2010-02-03 Atlas Genetics Ltd Microbial assay
US8759037B2 (en) 2010-02-23 2014-06-24 Illumina Cambridge Limited Amplification methods to minimise sequence specific bias
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
MX346994B (es) 2010-10-12 2017-04-06 Monsanto Technology Llc Planta y semilla de soja correspondiente al evento transgénico mon87712 y métodos para su detección.
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
WO2012083137A1 (en) 2010-12-17 2012-06-21 Monsanto Technology Llc Methods for improving competency of plant cells
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
WO2012149193A2 (en) 2011-04-29 2012-11-01 Monsanto Technology Llc Diagnostic molecular markers for seed lot purity traits in soybeans
EP2704554B1 (en) 2011-05-02 2019-04-10 The Board of Regents of the University of Nebraska Plants with useful traits and related methods
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2714935B1 (en) 2011-05-27 2017-03-15 Genapsys Inc. Systems and methods for genetic and biological analysis
JP6223332B2 (ja) 2011-06-30 2017-11-01 モンサント テクノロジー エルエルシー 形質転換事象kk179−2に対応するアルファルファ植物および種子、ならびにその検出方法
US9670540B2 (en) 2011-07-21 2017-06-06 Cornell University Methods and devices for DNA sequencing and molecular diagnostics
WO2013033611A1 (en) 2011-08-31 2013-03-07 Monsanto Technology Llc Methods and compositions for watermelon firmness
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
WO2013082619A1 (en) 2011-12-01 2013-06-06 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
JP5958034B2 (ja) * 2012-04-11 2016-07-27 富士レビオ株式会社 分子ビーコン型プローブを用いた標的核酸の検出方法
EP3461910B1 (en) 2012-04-19 2020-08-26 Life Technologies Corporation Nucleic acid amplification
SG11201406717RA (en) 2012-04-19 2014-11-27 Life Technologies Corp Nucleic acid amplification
CN114214394A (zh) 2012-06-08 2022-03-22 爱奥尼安技术公司 核苷酸扩增反应
DE102012014981B3 (de) * 2012-07-26 2013-07-18 Sartorius Stedim Biotech Gmbh Verfahren zur Differenzierung zwischen lebenden und toten Zellen
WO2014031163A1 (en) 2012-08-24 2014-02-27 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
US10314253B2 (en) 2012-12-04 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and compositions for watermelon sex expression
WO2014145291A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
SG11201507261WA (en) 2013-03-15 2015-10-29 Theranos Inc Nucleic acid amplification
US10294489B2 (en) 2013-03-15 2019-05-21 Board Of Trustees Of Southern Illinois University Soybean resistant to cyst nematodes
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
SG11201507272RA (en) 2013-03-15 2015-10-29 Theranos Inc Nuclei acid amplification
US10059999B2 (en) 2013-06-10 2018-08-28 Monsanto Technology Llc Molecular markers associated with soybean tolerance to low iron growth conditions
CA2922926A1 (en) 2013-09-04 2015-03-12 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
CN103421777B (zh) * 2013-09-11 2015-05-13 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
NZ630628A (en) 2013-10-08 2015-04-24 Seminis Vegetable Seeds Inc Methods and compositions for peronospora resistance in spinach
WO2015053998A1 (en) 2013-10-09 2015-04-16 Monsanto Technology Llc Transgenic corn event mon87403 and methods for detection thereof
EP3063272A2 (en) 2013-10-30 2016-09-07 Green Life Biotech, LLC Pathogenesis quantification systems and treatment methods for citrus greening blight
NZ630717A (en) 2013-11-27 2016-04-29 Seminis Vegetable Seeds Inc Disease resistance loci in onion
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
CN106103713B (zh) 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
EA202091497A3 (ru) 2014-02-21 2020-12-30 Зингента Партисипейшнс Аг Генетические локусы, связанные с повышенной фертильностью у маиса
NZ630710A (en) 2014-02-27 2016-03-31 Seminis Vegetable Seeds Inc Compositions and methods for peronospora resistance in spinach
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
GB201410022D0 (en) * 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
EP3155127B1 (en) 2014-06-13 2020-07-22 Life Technologies Corporation Multiplex nucleic acid amplification
US10316369B2 (en) 2014-06-27 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and assays for male sterile watermelon
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
CN107208075B (zh) 2014-09-29 2021-08-24 伊卢米纳剑桥有限公司 重组酶突变体
EP3005862A1 (en) 2014-10-10 2016-04-13 Seminis Vegetable Seeds, Inc. Melon plants with improved disease tolerance
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
HUE053541T2 (hu) 2015-04-28 2021-07-28 Monsanto Technology Llc Eljárások és készítmények brachitikus kukoricanövények elõállítására
CA2981819A1 (en) 2015-04-30 2016-11-03 Monsanto Technology Llc Methods for producing canola plants with clubroot resistance and compositions thereof
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft シトラコン酸無水物によって微生物を不活性化する方法
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
WO2017031059A1 (en) 2015-08-18 2017-02-23 Monsanto Technology Llc Methods for producing cotton plants with enhanced drought tolerance and compositions thereof
US10448595B2 (en) 2015-09-03 2019-10-22 Seminis Vegetable Seeds, Inc. Downy mildew resistant lettuce plants
US10858709B2 (en) 2015-09-10 2020-12-08 Monsanto Technology Llc Methods for producing corn plants with downy mildew resistance and compositions thereof
GB201519565D0 (en) 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
WO2017100283A1 (en) 2015-12-09 2017-06-15 Life Technologies Corporation Detection and quantification of nucleic acid molecules associated with a surface
BR112018012039A2 (pt) 2015-12-18 2018-12-04 Monsanto Technology Llc métodos para produção de plantas de milho com resistência à helmintosporiose e composições dos mesmos
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
EP3402880B1 (en) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophilic dna polymerase mutants
EP4556571A3 (en) 2016-02-26 2025-09-03 Abbott Diagnostics Scarborough, Inc. Redox labeled oligonucleotide probes and their use
AU2017228401B2 (en) 2016-03-04 2023-07-27 Abbott Diagnostics Scarborough, Inc. Automated nested recombinase polymerase amplification
KR102168695B1 (ko) 2016-03-28 2020-10-21 일루미나, 인코포레이티드 재조합효소 돌연변이체
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
EP3496528A4 (en) 2016-08-11 2020-03-18 Monsanto Technology LLC METHODS AND COMPOSITIONS FOR PRODUCING CORN PLANTS WITH RESISTANCE TO LATE FAILURE
AU2017325833B2 (en) 2016-09-15 2022-06-16 Abbott Laboratories Devices and methods for sample analysis
AU2017232187B2 (en) 2016-09-30 2023-11-09 Seminis Vegetable Seeds, Inc. Xanthomonas resistant brassica oleracea plants
WO2018071522A1 (en) 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
CN106701738B (zh) * 2016-11-10 2020-05-08 中国科学院成都生物研究所 一种等温解开双链dna及制备单链dna的方法
AU2018227436A1 (en) 2017-02-28 2019-09-12 Abbott Diagnostics Scarborough, Inc. Microfluidic devices and related methods
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
RU2020100221A (ru) 2017-08-31 2021-09-30 Иониан Текнолоджис, Ллс Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса
WO2019055780A1 (en) 2017-09-14 2019-03-21 Alere San Diego Inc. POLYMERASE RECOMBINASE AMPLIFICATION DETECTION USING DOUBLE HAPTEN PROBE
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
EP4310196A3 (en) 2017-11-07 2024-04-24 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
CN108034763B (zh) * 2017-12-21 2021-10-22 北京卓诚惠生生物科技股份有限公司 检测甲型肝炎和戊型肝炎病毒的引物、探针及试剂盒
US10597714B2 (en) 2017-12-29 2020-03-24 Clear Labs, Inc. Automated priming and library loading device
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
US10101328B1 (en) 2017-12-29 2018-10-16 Clear Labs, Inc. Detection of microorganisms in food samples and food processing facilities
US11268102B2 (en) 2018-05-16 2022-03-08 University Of Florida Research Foundation, Incorporated Compositions and methods for identifying and selecting brachytic locus in solanaceae
CN112292460A (zh) 2018-06-12 2021-01-29 主基因有限公司 核酸扩增方法
CN108410954A (zh) * 2018-07-10 2018-08-17 默禾医疗科技(上海)有限公司 一种室温扩增反应体系中反应缓冲液的制配方法
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
WO2020092255A1 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Accelerated polymerase chain reaction
AU2019400090B2 (en) 2018-12-14 2025-11-27 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
JP7296409B2 (ja) 2018-12-17 2023-06-22 イルミナ ケンブリッジ リミテッド ポリヌクレオチドシーケンシングに使用するための組成物
EP3899036A1 (en) 2018-12-17 2021-10-27 Illumina Cambridge Limited Primer oligonucleotide for sequencing
CA3145881A1 (en) 2019-01-15 2020-07-23 Seminis Vegetable Seeds, Inc. Green bean plants with improved resistance to sclerotinia sclerotiorum and genetic markers therefor
EP4403648B1 (en) 2019-05-03 2025-08-20 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
KR20220062302A (ko) 2019-08-21 2022-05-16 라이프 테크놀로지스 코포레이션 시퀀싱을 위한 시스템 및 방법
US11542513B2 (en) 2019-09-26 2023-01-03 Seminis Vegetable Seeds, Inc. Lettuce plants having resistance to Nasonovia ribisnigri biotype Nr:1
GB201916379D0 (en) 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
WO2021228839A1 (en) 2020-05-11 2021-11-18 Deutsches Krebsforschungszentrum Polynucleotide detection by cas nucleases
AU2021204717A1 (en) 2020-07-15 2022-02-03 Seminis Vegetable Seeds, Inc. Green Bean Plants with Improved Disease Resistance
US20220170093A1 (en) 2020-11-16 2022-06-02 Life Technologies Corporation System and method for sequencing
EP4305198A1 (en) 2021-03-09 2024-01-17 Etablissement Français du Sang Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination
US20240210431A1 (en) 2021-04-29 2024-06-27 Abbott Laboratories Systems and methods for pooling samples for high-throughput analysis
WO2022266946A1 (zh) * 2021-06-24 2022-12-29 黄婉秋 检测病原体rna、及动物或人类基因rna的试剂盒及其应用
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4373965A1 (en) 2021-07-21 2024-05-29 DNAe Diagnostics Limited Method and system comprising a cartridge for sequencing target polynucleotides
ES3037735T3 (en) 2021-08-03 2025-10-06 Univ Wageningen Argonaute-based nucleic acid detection system
US12480157B2 (en) 2021-09-30 2025-11-25 Illumina, Inc. Polynucleotide sequencing
CN114237548B (zh) * 2021-11-22 2023-07-18 南京大学 基于非易失性存储器阵列的复数点乘运算的方法及系统
CN113981045B (zh) 2021-11-23 2025-09-16 广州达安基因股份有限公司 制备恒温扩增混合酶系的方法
CN114085891A (zh) 2021-11-23 2022-02-25 广州达安基因股份有限公司 基于重组酶聚合酶扩增技术的逆转录扩增系统和方法
US20230193310A1 (en) 2021-12-10 2023-06-22 Seminis Vegetabe Seeds, Inc. Lettuce plants having resistance to downy mildew
US20230404003A1 (en) 2022-06-21 2023-12-21 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucumber mosaic virus
EP4565720A1 (en) 2022-08-04 2025-06-11 Abbott Laboratories Assays for detecting monkeypox virus
AU2023353465A1 (en) 2022-09-29 2024-10-03 Illumina, Inc. Dynamic optical system calibration
US20240344079A1 (en) 2023-04-14 2024-10-17 Seminis Vegetable Seeds, Inc. Methods and compositions for peronospora resistance in spinach
WO2025049450A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting bk virus (bkv)
WO2025049451A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting cytomegalovirus (cmv) or epstein-bar virus (ebv)
WO2025111147A1 (en) 2023-11-21 2025-05-30 Abbott Laboratories Two-dimensional matrix droplet array
WO2025144626A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins
WO2025144744A1 (en) 2023-12-29 2025-07-03 Life Technologies Corporation Enrichment of clonal substrates
WO2025188823A1 (en) 2024-03-07 2025-09-12 Illumina, Inc. Immersion microscopy arrangement for sequencing system with rapid scanning
WO2025244815A1 (en) 2024-05-21 2025-11-27 Illumina, Inc. Flow cell assemblies and related systems and methods

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US603585A (en) 1898-05-03 cbowell
US5430136A (en) 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5635347A (en) * 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB8903627D0 (en) 1989-02-17 1989-04-05 Unilever Plc Assays
ES2089038T3 (es) * 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
AU661505B2 (en) * 1990-05-07 1995-07-27 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double D-loop formation
CA2087724C (en) 1990-07-24 2003-09-16 John J. Sninsky Reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556751A (en) 1991-04-25 1996-09-17 Amoco Corporation Selective amplification system using Q-β replicase
EP0524349A1 (en) * 1991-07-08 1993-01-27 Opticon Sensors Europe B.V. Dual-focus scanner and such a scanner used as wandtype symbol code reader
CA2077135A1 (en) * 1991-08-30 1993-03-01 Joh-E Ikeda A method of dna amplification
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5656430A (en) 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5731150A (en) 1995-11-01 1998-03-24 Chiron Diagnostic Corporation IS6110 based molecular detection of mycobacterium tuberculosis
CA2257197A1 (en) * 1996-06-03 1997-12-11 Jeffrey Edberg Fc receptor polymorphism
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
WO1998008975A1 (en) * 1996-08-29 1998-03-05 Daikin Industries, Ltd. Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
ATE214104T1 (de) 1998-04-22 2002-03-15 Entpr Ie Trd As Bioresearch Ie Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst
WO1999060158A1 (en) 1998-05-19 1999-11-25 Laboratory Of Molecular Biophotonics Solid phase for detecting nucleic acid and method for detecting nucleic acid
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
EP1149175A2 (en) 1999-01-11 2001-10-31 President And Fellows Of Harvard College Isothermal amplification of dna
US6699693B1 (en) * 1999-02-04 2004-03-02 Sloan-Kettering Institute For Cancer Research Process for DNA replication
WO2000046408A1 (en) 1999-02-04 2000-08-10 Sloan-Kettering Institute For Cancer Research Process for dna replication
US6387621B1 (en) 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
NO314091B1 (no) * 2000-01-12 2003-01-27 Biotec Pharmacon Asa Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
WO2001070947A2 (en) 2000-03-20 2001-09-27 Maxygen, Inc. Method for generating recombinant dna molecules in complex mixtures
DK1715063T3 (da) 2000-03-29 2011-05-16 Lgc Ltd Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
US20020061530A1 (en) 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
US6379899B1 (en) * 2001-03-13 2002-04-30 Discoverx Isothermal exponential RNA amplification in complex mixtures
DE60226856D1 (de) * 2001-04-20 2008-07-10 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
WO2004022701A2 (en) 2001-07-15 2004-03-18 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
US7112423B2 (en) 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
EP1420069A4 (en) 2001-08-20 2005-11-02 Takara Bio Inc nucleic acid amplification
AU2002341898A2 (en) 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
KR100445560B1 (ko) 2001-10-31 2004-08-21 (주)바이오넥스 핵산 또는 생물학적 물질을 분리하기 위한 키트의 제조방법과, 그 방법에 의해 제조된 키트와, 그 키트를사용하는 장치
US7244562B2 (en) 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
AU2003215391B2 (en) 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US20040137456A1 (en) * 2002-04-04 2004-07-15 Hiroki Yokota Method for identifying and characterizing individual dna molecules
US20030228611A1 (en) 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US6713262B2 (en) 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
WO2004007078A1 (en) 2002-07-12 2004-01-22 British Biocell International Limited Lateral flow assay device and method
US20040038213A1 (en) 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
AU2003274914A1 (en) 2002-08-21 2004-03-11 Epoch Biosciences, Inc. Abasic site endonuclease assay
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
WO2004081224A2 (en) * 2003-03-11 2004-09-23 Gene Check, Inc. Reca-assisted allele specific oligonucleotide extension method
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
US20050191682A1 (en) 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
JP5026958B2 (ja) 2004-06-01 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2006040187A2 (en) 2004-10-11 2006-04-20 Epigenomics Ag A method for the carry-over protection in dna amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids
US20060110765A1 (en) 2004-11-23 2006-05-25 Wang Xiao B Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method
US20070042427A1 (en) 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
EP1896610A2 (en) 2005-05-03 2008-03-12 Handylab, Inc. Lyophilized pellets
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
WO2007008997A2 (en) 2005-07-12 2007-01-18 The Board Of Trustees Of The Leland Stanford Junior University Template specific inhibition of pcr
CA2616241C (en) 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CA2650993C (en) * 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
US8357488B2 (en) 2007-05-18 2013-01-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primers and probes for the detection of streptococcus pneumoniae
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
ES2320840B1 (es) 2007-11-27 2010-07-01 Nelson Alfonso Egued Tubo humectador de doble camara.
JP2009139487A (ja) 2007-12-04 2009-06-25 Nippon Sheet Glass Co Ltd 正立等倍レンズアレイプレート
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
CN114214394A (zh) 2012-06-08 2022-03-22 爱奥尼安技术公司 核苷酸扩增反应

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