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WO2006030455A8 - Dna polymerases having strand displacement activity - Google Patents

Dna polymerases having strand displacement activity

Info

Publication number
WO2006030455A8
WO2006030455A8 PCT/IS2005/000022 IS2005000022W WO2006030455A8 WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8 IS 2005000022 W IS2005000022 W IS 2005000022W WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8
Authority
WO
WIPO (PCT)
Prior art keywords
polymerases
strand displacement
dna polymerases
displacement activity
species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IS2005/000022
Other languages
French (fr)
Other versions
WO2006030455A1 (en
Inventor
Sigridur Hjorleifsdottir
Sveinn Ernstson
Thorarinn Blondal
Arnthor Aevarsson
Gudmundur Oli Hreggvidsson
Jakob Kristjansson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prokaria Ltd
Original Assignee
Prokaria Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ltd filed Critical Prokaria Ltd
Priority to US11/662,879 priority Critical patent/US20080311626A1/en
Publication of WO2006030455A1 publication Critical patent/WO2006030455A1/en
Anticipated expiration legal-status Critical
Publication of WO2006030455A8 publication Critical patent/WO2006030455A8/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides novel strand displacement DNA polymerases which can be used in a rapid and efficient strand displacement amplification reactions. The polymerases are significantly more thermostable than prior art polymerase and retain high activity at elevated temperatures. Also disclosed are genes encoding the polymerases and vectors comprising the genes. Representative polymerases of the invention are obtainable from bacterial strains of the species Thermus antranikianii and Thermus brockianus and also from environmental samples with isolation of the source species.
PCT/IS2005/000022 2004-09-17 2005-09-19 Dna polymerases having strand displacement activity Ceased WO2006030455A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/662,879 US20080311626A1 (en) 2004-09-17 2005-09-19 Dna Polymerases Having Strand Displacement Activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61056204P 2004-09-17 2004-09-17
IS746104 2004-09-17
US60/610,562 2004-09-17
IS7461 2004-09-17

Publications (2)

Publication Number Publication Date
WO2006030455A1 WO2006030455A1 (en) 2006-03-23
WO2006030455A8 true WO2006030455A8 (en) 2007-05-03

Family

ID=37969691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2005/000022 Ceased WO2006030455A1 (en) 2004-09-17 2005-09-19 Dna polymerases having strand displacement activity

Country Status (1)

Country Link
WO (1) WO2006030455A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1914303A1 (en) * 2006-10-09 2008-04-23 Qiagen GmbH Thermus eggertssonii DNA polymerases
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
JP2010161935A (en) * 2009-01-13 2010-07-29 Fujifilm Corp Method for reducing dispersion in nucleic acid amplification reaction
EP2981609B1 (en) 2013-04-05 2020-04-29 Bioron GmbH Novel dna-polymerases
ES2935285T3 (en) 2014-06-06 2023-03-03 Univ Cornell Method for identification and enumeration of changes in nucleic acid sequence, expression, copying, or methylation of DNA, using combined nuclease, ligase, polymerase, and sequencing reactions
GB201721053D0 (en) 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
US20220056498A1 (en) 2019-01-25 2022-02-24 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
CA3156310A1 (en) * 2019-10-01 2021-04-08 Universitetet I Tromso - Norges Arktiske Universitet Marine dna polymerase i
CN115176027A (en) 2019-12-12 2022-10-11 哈佛大学校长及研究员协会 Compositions and methods for light-directed biomolecular barcoding
JP2023545038A (en) 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Methods, compositions, kits, and systems for signal-on detection of neutralizing targets
WO2022082200A2 (en) * 2020-10-16 2022-04-21 Geaenzymes Co. Biochemical saturation of molecules and its use
WO2022094415A1 (en) * 2020-10-30 2022-05-05 Singular Genomics Systems, Inc. Methods and compositions for reducing nucleotide impurities

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009689A1 (en) * 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
AU2002346498A1 (en) * 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases

Also Published As

Publication number Publication date
WO2006030455A1 (en) 2006-03-23

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