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WO2003002727A3 - Hammerhead ribozyme specific for the stearoyl-acp desaturrase of different oleaginous plants - Google Patents

Hammerhead ribozyme specific for the stearoyl-acp desaturrase of different oleaginous plants Download PDF

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Publication number
WO2003002727A3
WO2003002727A3 PCT/EP2002/006585 EP0206585W WO03002727A3 WO 2003002727 A3 WO2003002727 A3 WO 2003002727A3 EP 0206585 W EP0206585 W EP 0206585W WO 03002727 A3 WO03002727 A3 WO 03002727A3
Authority
WO
WIPO (PCT)
Prior art keywords
stearoyl
acp
desaturrase
hammerhead ribozyme
oleaginous plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/006585
Other languages
French (fr)
Other versions
WO2003002727A2 (en
Inventor
Ambrosio Caterina D
Adriana Lucia Stigliani
Ivana Marino
Leonarda Salfi
Francesco Cellini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metapontum Agrobios SCARL
Original Assignee
Metapontum Agrobios SCARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metapontum Agrobios SCARL filed Critical Metapontum Agrobios SCARL
Priority to AU2002345041A priority Critical patent/AU2002345041A1/en
Publication of WO2003002727A2 publication Critical patent/WO2003002727A2/en
Publication of WO2003002727A3 publication Critical patent/WO2003002727A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A Hammerhead ribozyme is described, which is capable of modulating the expression of the gene encoding the stearoyl-ACP desaturase enzyme in different oleaginous plants, a vector containing the sequence which encodes said ribozyme and its use for the production of transgenic plants with an improved content of stearic acid.
PCT/EP2002/006585 2001-06-28 2002-06-13 Hammerhead ribozyme specific for the stearoyl-acp desaturrase of different oleaginous plants Ceased WO2003002727A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002345041A AU2002345041A1 (en) 2001-06-28 2002-06-13 Hammerhead ribozyme specific for the stearoyl-acp desaturrase of different oleaginous plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI01A001364 2001-06-28
IT2001MI001364A ITMI20011364A1 (en) 2001-06-28 2001-06-28 ROBOZIMA HAMMERHEAD SPECIFIC FOR STEAROYL-ACP DESATURESI OF DIFFERENT OILAGINOUS PLANTS

Publications (2)

Publication Number Publication Date
WO2003002727A2 WO2003002727A2 (en) 2003-01-09
WO2003002727A3 true WO2003002727A3 (en) 2003-11-20

Family

ID=11447954

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/006585 Ceased WO2003002727A2 (en) 2001-06-28 2002-06-13 Hammerhead ribozyme specific for the stearoyl-acp desaturrase of different oleaginous plants

Country Status (3)

Country Link
AU (1) AU2002345041A1 (en)
IT (1) ITMI20011364A1 (en)
WO (1) WO2003002727A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005852A1 (en) * 1987-12-15 1989-06-29 Macphillamy Cummins & Gibson Ribozymes
WO1999028485A2 (en) * 1997-12-03 1999-06-10 Gene Shears Pty. Ltd. Ribozymes capable of conferring resistance to potyvirus infection, and plants expressing said ribozymes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005852A1 (en) * 1987-12-15 1989-06-29 Macphillamy Cummins & Gibson Ribozymes
WO1999028485A2 (en) * 1997-12-03 1999-06-10 Gene Shears Pty. Ltd. Ribozymes capable of conferring resistance to potyvirus infection, and plants expressing said ribozymes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CARRIERO F ET AL: "Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.).", THEORETICAL AND APPLIED GENETICS, vol. 104, no. 2-3, February 2002 (2002-02-01), February, 2002, pages 301 - 307, XP002236910, ISSN: 0040-5752 *
DATABASE EMBL [online] 1 April 1998 (1998-04-01), XP002236912, retrieved from EMBL Database accession no. AL022337 *
DATABASE EMBL [online] 7 September 2000 (2000-09-07), XP002236911, retrieved from EMBL Database accession no. AX022818 *
HASELOFF J ET AL: "SIMPLE RNA ENZYMES WITH NEW AND HIGHLY SPECIFIC ENDORIBONUCLEASE ACTIVITIES", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 334, 18 August 1988 (1988-08-18), pages 585 - 591, XP002029776, ISSN: 0028-0836 *
MERLO ANN OWENS ET AL: "Ribozymes targeted to stearoyl-ACP DELTA9 desaturase mRNA produce heritable increases of stearic acid in transgenic maize leaves.", PLANT CELL, vol. 10, no. 10, October 1998 (1998-10-01), pages 1603 - 1621, XP002236909, ISSN: 1040-4651 *
PERRIMAN R ET AL: "EXTENDED TARGET-SITE SPECIFICITY FOR A HAMMERHAED RIBOZYME", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 113, no. 2, 1992, pages 157 - 163, XP000267805, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
AU2002345041A1 (en) 2003-03-03
WO2003002727A2 (en) 2003-01-09
ITMI20011364A0 (en) 2001-06-28
ITMI20011364A1 (en) 2002-12-28

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