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WO2003068945A3 - Detection of molecular interaction by reactivation of an auto-inhibited responder (rair) - Google Patents

Detection of molecular interaction by reactivation of an auto-inhibited responder (rair) Download PDF

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Publication number
WO2003068945A3
WO2003068945A3 PCT/US2003/004925 US0304925W WO03068945A3 WO 2003068945 A3 WO2003068945 A3 WO 2003068945A3 US 0304925 W US0304925 W US 0304925W WO 03068945 A3 WO03068945 A3 WO 03068945A3
Authority
WO
WIPO (PCT)
Prior art keywords
responder
ensemble members
reactivation
auto
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/004925
Other languages
French (fr)
Other versions
WO2003068945A2 (en
Inventor
Robert R Balint
Jeng-Horng Her
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Humanigen Inc
Original Assignee
Kalobios Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/076,845 external-priority patent/US20030157579A1/en
Priority claimed from US10/208,730 external-priority patent/US7335478B2/en
Application filed by Kalobios Inc filed Critical Kalobios Inc
Priority to EP03713528A priority Critical patent/EP1570073A4/en
Priority to AU2003217575A priority patent/AU2003217575A1/en
Publication of WO2003068945A2 publication Critical patent/WO2003068945A2/en
Anticipated expiration legal-status Critical
Publication of WO2003068945A3 publication Critical patent/WO2003068945A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention provides methods and systems for linking the functional activation of a responder molecule to the interaction of two or more binding ensemble members of interest either in vitro or in vivo, and thereby producing a signal, phenotype, or other function output in response to the interaction of the binding ensemble members. These systems based on the reactivation orf an auto-inhibitor of the inhibitor, or 'reactivator' of the responder, and interacting components. Two binding ensemble members are complexed to the other components of the system in such a way that interaction of the binding ensemble members, either directly or via addition ensemble members, causes a shift in the equilibrium of inhibitor binding from the responder to the reactivator, thereby functionally reactivating the responder (Figure 1).
PCT/US2003/004925 2002-02-14 2003-02-14 Detection of molecular interaction by reactivation of an auto-inhibited responder (rair) Ceased WO2003068945A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03713528A EP1570073A4 (en) 2002-02-14 2003-02-14 Detection of molecular interaction by reactivation of an auto-inhibited responder (rair)
AU2003217575A AU2003217575A1 (en) 2002-02-14 2003-02-14 Detection of molecular interaction by reactivation of an auto-inhibited responder (rair)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US10/076,845 2002-02-14
US10/076,845 US20030157579A1 (en) 2002-02-14 2002-02-14 Molecular sensors activated by disinhibition
US37376502P 2002-04-18 2002-04-18
US37380202P 2002-04-18 2002-04-18
US60/373,765 2002-04-18
US60/373,802 2002-04-18
US37971802P 2002-05-10 2002-05-10
US60/379,718 2002-05-10
US10/208,730 2002-07-29
US10/208,730 US7335478B2 (en) 2002-04-18 2002-07-29 Reactivation-based molecular interaction sensors

Publications (2)

Publication Number Publication Date
WO2003068945A2 WO2003068945A2 (en) 2003-08-21
WO2003068945A3 true WO2003068945A3 (en) 2005-07-14

Family

ID=27739444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/004925 Ceased WO2003068945A2 (en) 2002-02-14 2003-02-14 Detection of molecular interaction by reactivation of an auto-inhibited responder (rair)

Country Status (3)

Country Link
EP (1) EP1570073A4 (en)
AU (1) AU2003217575A1 (en)
WO (1) WO2003068945A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2688912B1 (en) * 2011-03-23 2016-11-09 Agency For Science, Technology And Research Recombinant protein biosensors and a method for detecting the presence of an analyte molecule
WO2016065415A1 (en) * 2014-10-27 2016-05-06 The University Of Queensland Bimolecular autoinhibited biosensor
WO2018090094A1 (en) * 2016-11-16 2018-05-24 Universal Biosensors Pty Ltd Cleavage event transduction methods and products

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998044350A1 (en) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998044350A1 (en) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL.: "Highly sensitive biologial and chemical sensors based on reversibel fluorescence quenching in a conjugated polymer", PNAS, vol. 96, no. 22, October 1999 (1999-10-01), pages 12287 - 12292, XP002929398 *
JOHNSSON ET AL.: "Split ubiquitin as a sensor of protein interactions in vivo", PROC. NATL. ACAD. SCI. USA, vol. 91, October 1994 (1994-10-01), pages 10340 - 10344, XP002064564 *
ROSSI ET AL.: "Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation", PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 8405 - 8410, XP002064565 *
See also references of EP1570073A4 *

Also Published As

Publication number Publication date
EP1570073A2 (en) 2005-09-07
WO2003068945A2 (en) 2003-08-21
EP1570073A4 (en) 2006-01-18
AU2003217575A8 (en) 2003-09-04
AU2003217575A1 (en) 2003-09-04

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