[go: up one dir, main page]

WO2003068712A3 - Procede de preparation de biopuces a adn et leurs applications - Google Patents

Procede de preparation de biopuces a adn et leurs applications Download PDF

Info

Publication number
WO2003068712A3
WO2003068712A3 PCT/FR2003/000464 FR0300464W WO03068712A3 WO 2003068712 A3 WO2003068712 A3 WO 2003068712A3 FR 0300464 W FR0300464 W FR 0300464W WO 03068712 A3 WO03068712 A3 WO 03068712A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
biochips
production
detection
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2003/000464
Other languages
English (en)
Other versions
WO2003068712A2 (fr
Inventor
Pierre Nassoy
Marie-Claude Potier
M Luc Talini
Nathalie Gibelin
Jean Rossier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Curie
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut Curie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut Curie filed Critical Centre National de la Recherche Scientifique CNRS
Priority to CA002475696A priority Critical patent/CA2475696A1/fr
Priority to JP2003567847A priority patent/JP2005517902A/ja
Priority to US10/504,163 priority patent/US20050100905A1/en
Priority to AU2003226885A priority patent/AU2003226885A1/en
Priority to EP03739533A priority patent/EP1474368A2/fr
Publication of WO2003068712A2 publication Critical patent/WO2003068712A2/fr
Publication of WO2003068712A3 publication Critical patent/WO2003068712A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention est relative à un procédé de préparation d'une biopuce activée pour la fixation covalente de sondes oligonucléotidiques sur un support solide par l'intermédiaire d'un composé espaceur de type NHS-PEG-VS, ainsi que les biopuces susceptibles d'être obtenues par un tel procédé. L'invention comprend également des méthodes de détection d'acides nucléiques dans un échantillon ou des méthodes de criblage de composés capables de se fixer spécifiquement sur des sondes oligonucléotidiques dans lesquelles sont mises en oeuvre les biopuces selon l'invention. La présente invention a aussi pour objet des kits de détection, d'analyse quantitative ou qualitative d'acides nucléiques dans un échantillon, comprenant de telles biopuces ainsi que l'utilisation de ces dernières comme matrice d'affinité pour la purification d'acide nucléique, pour le séquençage d'acide nucléique, pour l'analyse qualitative ou quantitative de l'expression de gènes ou encore pour l'étude et la détection de polymorphisme génétique.
PCT/FR2003/000464 2002-02-13 2003-02-13 Procede de preparation de biopuces a adn et leurs applications Ceased WO2003068712A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002475696A CA2475696A1 (fr) 2002-02-13 2003-02-13 Nouveau procede de preparation de biopuces a adn et leurs applications
JP2003567847A JP2005517902A (ja) 2002-02-13 2003-02-13 Ｄｎａバイオチップ作製法およびその適用法
US10/504,163 US20050100905A1 (en) 2002-02-13 2003-02-13 Novel method for production of dna biochips and applications thereof
AU2003226885A AU2003226885A1 (en) 2002-02-13 2003-02-13 Novel method for production of dna biochips and applications thereof
EP03739533A EP1474368A2 (fr) 2002-02-13 2003-02-13 Procede de preparation de biopuces a adn et leurs applications

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0201791A FR2835829B1 (fr) 2002-02-13 2002-02-13 Nouveau procede de preparation de biopuces a adn ou a proteines et leurs applications
FR02/01791 2002-02-13

Publications (2)

Publication Number Publication Date
WO2003068712A2 WO2003068712A2 (fr) 2003-08-21
WO2003068712A3 true WO2003068712A3 (fr) 2004-03-25

Family

ID=27620174

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/000464 Ceased WO2003068712A2 (fr) 2002-02-13 2003-02-13 Procede de preparation de biopuces a adn et leurs applications

Country Status (7)

Country Link
US (1) US20050100905A1 (fr)
EP (1) EP1474368A2 (fr)
JP (1) JP2005517902A (fr)
AU (1) AU2003226885A1 (fr)
CA (1) CA2475696A1 (fr)
FR (1) FR2835829B1 (fr)
WO (1) WO2003068712A2 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7361767B2 (en) 2003-11-14 2008-04-22 3M Innovative Properties Company N-sulfonyldicarboximide containing tethering compounds
US7423155B2 (en) 2003-11-14 2008-09-09 3M Innovative Properties Company N-sulfonyldicarboximide containing tethering compounds
US7943388B2 (en) 2003-11-14 2011-05-17 3M Innovative Properties Company Acoustic sensors and methods
US7342082B2 (en) 2004-12-17 2008-03-11 3M Innovative Properties Company Soluble polymers as amine capture agents and methods
AU2004312384A1 (en) * 2003-12-30 2005-07-21 3M Innovative Properties Company Acoustic sensors and methods
US7402678B2 (en) 2004-12-17 2008-07-22 3M Innovative Properties Company Multifunctional amine capture agents
US7544754B2 (en) 2005-09-30 2009-06-09 3M Innovative Properties Company Crosslinked polymers with amine binding groups
US7544755B2 (en) 2005-09-30 2009-06-09 3M Innovative Properties Company Crosslinked polymers with amine binding groups
US7544756B2 (en) 2005-09-30 2009-06-09 3M Innovative Properties Company Crosslinked polymers with amine binding groups
WO2008039998A2 (fr) * 2006-09-28 2008-04-03 President And Fellows Of Harvard College Procédés de séquençage d'adn
WO2010151714A2 (fr) * 2009-06-24 2010-12-29 Life Technologies Corporation Réseaux moléculaires
CN104093425A (zh) * 2011-12-13 2014-10-08 伊缪诺金公司 N-羟基琥珀酰亚胺用于改善缀合物的稳定性的用途
CN104968403A (zh) 2012-09-17 2015-10-07 格雷斯公司 色谱介质和装置
CN104768536B (zh) 2012-09-17 2019-03-29 格雷斯公司 官能化微粒状载体材料和其制备和使用方法
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices
ES2929099T3 (es) 2014-05-02 2022-11-24 Grace W R & Co Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado
PL3302784T3 (pl) 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
SG11202106282RA (en) * 2018-12-12 2021-07-29 Bgi Shenzhen Biochip, method of preparation and use thereof
WO2022061455A1 (fr) * 2020-09-22 2022-03-31 Mcmaster University Interface de biodétection à surface imprégnée de lubrifiant, procédés de préparation et utilisations associées
CN119391513B (zh) * 2025-01-06 2025-05-16 天津中合基因科技有限公司 一种生物芯片、生物芯片的制备方法及dna合成方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034326A1 (fr) * 1994-06-14 1995-12-21 Tadahiko Kohno Reactifs de couplage de derives de polyethyleneglycol et composes formes avec ces derniers
WO1997041897A1 (fr) * 1996-05-09 1997-11-13 Virion Systems, Inc. Vaccins a base de conjugues proteine/polysaccharide prepares a l'aide de vinylsulfones homobifonctionnelles et heterobifonctionnelles
EP1106603A2 (fr) * 1999-12-06 2001-06-13 Fuji Photo Film Co., Ltd. Puce d'ADN et support réactif solide
WO2002042426A2 (fr) * 2000-11-10 2002-05-30 University Of Utah Research Foundation Systeme de transporteur pour apport specifique de genes au niveau de la paroi arterielle

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0916981B1 (fr) * 1997-11-17 2004-07-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Système et procédé de spectroscopie confocale
JPH11242189A (ja) * 1997-12-25 1999-09-07 Olympus Optical Co Ltd 像形成法、像形成装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034326A1 (fr) * 1994-06-14 1995-12-21 Tadahiko Kohno Reactifs de couplage de derives de polyethyleneglycol et composes formes avec ces derniers
WO1997041897A1 (fr) * 1996-05-09 1997-11-13 Virion Systems, Inc. Vaccins a base de conjugues proteine/polysaccharide prepares a l'aide de vinylsulfones homobifonctionnelles et heterobifonctionnelles
EP1106603A2 (fr) * 1999-12-06 2001-06-13 Fuji Photo Film Co., Ltd. Puce d'ADN et support réactif solide
WO2002042426A2 (fr) * 2000-11-10 2002-05-30 University Of Utah Research Foundation Systeme de transporteur pour apport specifique de genes au niveau de la paroi arterielle

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Polyethylene Glycol and Derivatives for Biomedical Applications", SHEARWATER CORPORATION - CATALOG 2001, XP002220431, Retrieved from the Internet <URL:www.shearwatercorp.com> [retrieved on 20021111] *
LUNDBERG, BO B. ET AL: "Conjugation of an anti-B-cell lymphoma monoclonal antibody, LL2, to long-circulating drug-carrier lipid emulsions", JOURNAL OF PHARMACY AND PHARMACOLOGY (1999), 51(10), 1099-1105, XP008010201 *
NAH J-W ET AL: "Artery wall binding peptide-poly(ethylene glycol)-grafted-poly(l-lysi ne)-based gene delivery to artery wall cells", JOURNAL OF CONTROLLED RELEASE, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 78, no. 1-3, 17 January 2002 (2002-01-17), pages 273 - 284, XP004329825, ISSN: 0168-3659 *
PERRET, EMILIE ET AL: "Versatile decoration of glass surfaces to probe individual protein-protein interactions and cellular adhesion", LANGMUIR (2002), 18(3), 846-854, 10 January 2002 (2002-01-10), XP002220430 *
WANG, TONG ET AL: "Protein stretching III: force-extension curves of tethered bovine carbonic anhydrase B to the silicon substrate under native, intermediate and denaturing conditions", JAPANESE JOURNAL OF APPLIED PHYSICS, PART 1: REGULAR PAPERS, SHORT NOTES & REVIEW PAPERS (1999), 38(6B), 3912-3917, XP001092793 *

Also Published As

Publication number Publication date
CA2475696A1 (fr) 2003-08-21
AU2003226885A1 (en) 2003-09-04
FR2835829B1 (fr) 2007-09-14
FR2835829A1 (fr) 2003-08-15
US20050100905A1 (en) 2005-05-12
WO2003068712A2 (fr) 2003-08-21
JP2005517902A (ja) 2005-06-16
EP1474368A2 (fr) 2004-11-10

Similar Documents

Publication Publication Date Title
WO2003068712A3 (fr) Procede de preparation de biopuces a adn et leurs applications
Sauer et al. Miniaturization in functional genomics and proteomics
WO2008115185A3 (fr) Utilisation de micromatrices pour la sélection de représentation génomique
US9850482B2 (en) Heterologous DNA barcoding method
Jain Applications of biochip and microarray systems in pharmacogenomics
EP2674492B1 (fr) Méthode de détection d&#39;un acide nucléique
WO2001075166A3 (fr) Compositions et methodes applicables a la detection et a la quantification d&#39;une expression genique
US20050181408A1 (en) Genetic analysis by sequence-specific sorting
WO2007002567A3 (fr) Séparation et concentration sélectives d&#39;acides nucléiques à partir d&#39;échantillons complexes
CA2595268A1 (fr) Purification et analyse de l&#39;adn sur des surfaces formees par nano-ingenierie
WO2000077260A8 (fr) Etablissement de profil genomique: procede rapide permettant d&#39;analyser un echantillon biologique complexe afin de deceler la presence de nombreux types d&#39;organismes
WO2004053105A3 (fr) Detection de snp directe avec adn non amplifie
WO2007115242A3 (fr) Sondes binaires pour analyse par fluorescence d&#39;acides nucléiques
Yüce et al. Systematic evolution of ligands by exponential enrichment for aptamer selection
KR101727111B1 (ko) 표적핵산 검출방법 및 키트
CN113026112A (zh) 用于构建人单细胞bcr测序文库的试剂盒及其应用
WO2008066873A3 (fr) Procédés d&#39;identification de ligands ciblant des molécules d&#39;acide nucléique et des motifs structurels d&#39;acide nucléique
AU2002226448B2 (en) A method and test kit for quantitative determination of variations in polynucleotide amounts in cell or tissue samples
JP2005532058A5 (fr)
WO2014094621A1 (fr) Kit et méthode de synthèse et de criblage d&#39;un composé &#39;tête de série&#39;
KR20130133456A (ko) 저급 지방족 포화 알데하이드류 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법
EP1323835A3 (fr) Méthode pour la détection d&#39;une espèce biologique dans un échantillon d&#39;essai et trousse utilisée pour la dite méthode
WO2006072368A3 (fr) Sonde pour detecter la presence d&#39;acides nucleiques
KR101383653B1 (ko) 프로피온알데히드 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법
Wawrzyniak-Turek et al. Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 10504163

Country of ref document: US

Ref document number: 2475696

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003567847

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003739533

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003739533

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2003739533

Country of ref document: EP