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WO2003040727A2 - Utilisation de nanoparticules oxydiques - Google Patents

Utilisation de nanoparticules oxydiques Download PDF

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Publication number
WO2003040727A2
WO2003040727A2 PCT/EP2002/011116 EP0211116W WO03040727A2 WO 2003040727 A2 WO2003040727 A2 WO 2003040727A2 EP 0211116 W EP0211116 W EP 0211116W WO 03040727 A2 WO03040727 A2 WO 03040727A2
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
oxidic
shell
oxide
core
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/011116
Other languages
German (de)
English (en)
Other versions
WO2003040727A3 (fr
Inventor
Dieter Vollath
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Karlsruher Institut fuer Technologie KIT
Original Assignee
Forschungszentrum Karlsruhe GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Karlsruhe GmbH filed Critical Forschungszentrum Karlsruhe GmbH
Publication of WO2003040727A2 publication Critical patent/WO2003040727A2/fr
Publication of WO2003040727A3 publication Critical patent/WO2003040727A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots

Definitions

  • the invention relates to the use of oxidic nanoparticles according to the first claim.
  • Fluorescence emitters are used for a number of applications.
  • organic dyes are used as laser dyes.
  • Other areas of application are in the fluorescence labeling of organic chemical substances or biological material.
  • powders made from semiconductors in particular powders made from gallium nitride (GaN), cadmium selenide (CdSe) and cadmium sulfide (CdS), emit fluorescence radiation.
  • GaN gallium nitride
  • CdSe cadmium selenide
  • CdS cadmium sulfide
  • organic fluorescent dyes there is a particular need for fluorescence emitters that emit in the ultraviolet.
  • organic fluorescent dyes see for example DE 34 08 028 AI.
  • organic fluorescent dyes are also often toxic and sometimes flammable.
  • Many organic fluorescent dyes also have to be suspended or dissolved in a toxic and flammable organic suspension or solvent.
  • the invention is based on the object of specifying suitable substances as fluorescence emitters which do not have the disadvantages described.
  • the substances should in particular be non-toxic or at most weakly toxic and should be able to be suspended in a permanent and stable suspension with water as the suspending agent.
  • the fluorescent light is said to visible and / or in the ultraviolet wavelength range.
  • the first-mentioned document describes particles with a core u. a. made of an oxide ceramic and a shell made of an organic polymer and a method for producing the particles.
  • the diameter of the core can be 3 nm to 100 nm and the thickness of the shell 1 n to 20 n.
  • Particles of a core consisting of an oxide ceramic and a shell consisting of a further oxide ceramic are known.
  • the diameter of the core should be between 3 and 50 nm and the thickness of the shell 1 to 5 nm. Methods for producing these nanoparticles are also described in the publications.
  • the nanoparticles which can be used according to the invention consist of a core which consists of an oxide ceramic and a shell.
  • the transition metal oxides Hf0 2 and Zr0 2 are particularly suitable as oxide ceramics for the core.
  • the shell around the core essentially serves only as a spacer; it reduces the interaction of the nuclei with one another and their agglomeration.
  • the shell itself can consist of any material that fulfills this task.
  • a polymer or a copolymer is preferably used as the material for the shell.
  • the polymer methacrylic methacrylate (PMMA) is particularly suitable as a shell.
  • oxide ceramics are also suitable as the material for the casing.
  • Aluminum- oxide (A1 2 0 3 ) is an oxide ceramic that can be used well as a covering material. The diameter and thickness of the core and shell and the manufacturing process are selected in accordance with the information in the two publications DE 196 38 601 Cl and DE 94 03 581 U1 mentioned above.
  • the oxidic nanoparticles which can be used according to the invention can emit fluorescent light in the range from below 800 nm to 200 nm. They prove to be very stable in every respect.
  • Ultraviolet light in particular light in the wavelength range from 200 nm to 400 nm, can be used as the excitation light.
  • the excitation light for the fluorescent radiation does not cause any deterioration in the emission properties even at high intensity.
  • the nanoparticles are inert for practically any length of time in normal surroundings.
  • stable fluorescent suspensions can be produced with water, so that the use of toxic and flammable organic suspending agents can be dispensed with and pumping around is unnecessary to avoid sedimentation.
  • An alternative form of use is that the nanoparticles are applied to a substrate in the form of a layer or a film.
  • Polymer-coated oxidic nanoparticles also have the advantage that proteins or other biologically important substances can be coupled to the organic polymer coating in a relatively simple manner, so that the nanoparticles can also be used as fluorescence emitters in biological studies.
  • oxide nanoparticles are preferred in which neither the core nor the shell is doped with foreign atoms, since they are easier and more constant to produce.
  • the formation of a line spectrum can also be strengthened and changed by doping the core of the nanoparticles with ions of different valence. The invention is explained in more detail below with reference to figures.
  • FIG. 1 shows the fluorescence spectrum of a pile of nanopowders, which on the one hand consists of non-coated hafnium dioxide and on the other hand consists of hafnium dioxide cores which are coated with aluminum oxide;
  • FIG. 2 shows the fluorescence spectrum of a pile of nanopowders with a zirconium dioxide core which is coated on the one hand with PMMA and on the other hand with aluminum oxide; 3 shows the fluorescence spectrum of a pile of nanopowder with a core made of zinc dioxide which is coated with PMMA; FIG. 4 gives explanatory information on the fluorescence spectrum according to FIG. 3.
  • the diameters of the particles with which the fluorescence spectra were recorded are 5 to 10 nm; the aluminum oxide or PMMA shell is approx. 0.5 to 5 nm thick.
  • the excitation light had a wavelength of 200 nm and 325 nm.
  • FIG. 1 shows the fluorescence spectra of non-coated nanoparticles made of hafnium dioxide and nanoparticles in which a core made of hafnium dioxide is provided with a cover made of aluminum oxide.
  • the fluorescent light intensity of the coated nanoparticles is considerably higher.
  • Zirconium oxide cores coated with PMMA show a significantly higher fluorescent light intensity than zirconium dioxide cores which are coated with aluminum oxide.
  • FIG. 3 shows the fluorescent light spectrum of zinc dioxide nuclei which are coated with PMMA.
  • FIG. 4 gives explanations for FIG. 3.
  • the fluorescence line in the range between 350 and 400 nm is clearly pronounced.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Materials Engineering (AREA)
  • Luminescent Compositions (AREA)
  • Physical Water Treatments (AREA)

Abstract

La présente invention concerne un émetteur de fluorescence pour la plage ultraviolette des longueurs d'ondes, qui est chimiquement stable et non toxique, ou tout au plus faiblement toxique, et qui peut être mis en suspension dans l'eau. Elle repose sur l'utilisation de nanoparticules oxydiques, constituées d'un noyau en céramique oxydique et d'une enveloppe, en tant qu'émetteurs de lumière fluorescente ultraviolette.
PCT/EP2002/011116 2001-11-08 2002-10-04 Utilisation de nanoparticules oxydiques Ceased WO2003040727A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10154988.1 2001-11-08
DE2001154988 DE10154988A1 (de) 2001-11-08 2001-11-08 Verwendung von oxidischen Nanoteilchen

Publications (2)

Publication Number Publication Date
WO2003040727A2 true WO2003040727A2 (fr) 2003-05-15
WO2003040727A3 WO2003040727A3 (fr) 2003-10-16

Family

ID=7705127

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/011116 Ceased WO2003040727A2 (fr) 2001-11-08 2002-10-04 Utilisation de nanoparticules oxydiques

Country Status (2)

Country Link
DE (1) DE10154988A1 (fr)
WO (1) WO2003040727A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027116A1 (fr) * 2005-09-01 2007-03-08 Instytut Wysokich Cisnien Polskiej Akademii Nauk Capteur d'oxygene par luminescence de dioxyde de zirconium
CN102792165A (zh) * 2010-02-25 2012-11-21 科康国际有限责任公司 用于测定生物流体和植物流体的抗氧化力的方法
WO2019000671A1 (fr) * 2017-06-28 2019-01-03 深圳市光峰光电技术有限公司 Céramique composite et procédé de préparation associé

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3718601A (en) * 1971-01-04 1973-02-27 Bell Telephone Labor Inc Fluorescent high alumina substrates
US4440831A (en) * 1981-06-30 1984-04-03 International Business Machines Corporation Zinc silicate phosphor particles and method for making them
US6048616A (en) * 1993-04-21 2000-04-11 Philips Electronics N.A. Corp. Encapsulated quantum sized doped semiconductor particles and method of manufacturing same
JP3410777B2 (ja) * 1993-09-13 2003-05-26 株式会社東芝 超微粒子無機蛍光体標識特異的結合物質およびこれを用いた検出方法
DE9403581U1 (de) * 1994-03-03 1994-04-28 Kernforschungszentrum Karlsruhe Gmbh, 76133 Karlsruhe Beschichtetes Nanopulver und Vorrichtung zu dessen Herstellung
DE19638601C1 (de) * 1996-09-20 1998-02-26 Karlsruhe Forschzent Verfahren zur Herstellung von Partikeln mit einem Kern und einer Hülle
AUPP004497A0 (en) * 1997-10-28 1997-11-20 University Of Melbourne, The Stabilized particles
US6251342B1 (en) * 1998-06-01 2001-06-26 Ford Global Technologies, Inc. Fluorescent fiber optic sensor element fabricated using sol-gel processing techniques
US6656588B1 (en) * 1998-12-01 2003-12-02 The Regents Of The University Of Michigan Ultrafine powders and their use as lasing media
US6245849B1 (en) * 1999-06-02 2001-06-12 Sandia Corporation Fabrication of ceramic microstructures from polymer compositions containing ceramic nanoparticles
AU5835801A (en) * 2000-05-05 2001-11-20 Bayer Ag Doped nanoparticles as biolabels

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027116A1 (fr) * 2005-09-01 2007-03-08 Instytut Wysokich Cisnien Polskiej Akademii Nauk Capteur d'oxygene par luminescence de dioxyde de zirconium
US7888658B2 (en) 2005-09-01 2011-02-15 Instytut Wysokich Cisnien Polskiej Akademii Nauk Zirconium dioxide luminescence oxygen sensor
CN102792165A (zh) * 2010-02-25 2012-11-21 科康国际有限责任公司 用于测定生物流体和植物流体的抗氧化力的方法
WO2019000671A1 (fr) * 2017-06-28 2019-01-03 深圳市光峰光电技术有限公司 Céramique composite et procédé de préparation associé
US11097984B2 (en) 2017-06-28 2021-08-24 Appotronics Corporation Limited Composite ceramic and preparation method therefor

Also Published As

Publication number Publication date
DE10154988A1 (de) 2003-05-28
WO2003040727A3 (fr) 2003-10-16

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