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WO2002034784A3 - An acyl coenzyme a carboxylase from streptomyces - Google Patents

An acyl coenzyme a carboxylase from streptomyces Download PDF

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Publication number
WO2002034784A3
WO2002034784A3 PCT/GB2001/004709 GB0104709W WO0234784A3 WO 2002034784 A3 WO2002034784 A3 WO 2002034784A3 GB 0104709 W GB0104709 W GB 0104709W WO 0234784 A3 WO0234784 A3 WO 0234784A3
Authority
WO
WIPO (PCT)
Prior art keywords
streptomyces
carboxylase
accase
polyketides
increasing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/004709
Other languages
French (fr)
Other versions
WO2002034784A2 (en
Inventor
Hugo Cesar Gramajo
Eduardo Jose Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Bioscience Ltd
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Priority to AU2001295782A priority Critical patent/AU2001295782A1/en
Priority to EP01976514A priority patent/EP1328551A2/en
Publication of WO2002034784A2 publication Critical patent/WO2002034784A2/en
Publication of WO2002034784A3 publication Critical patent/WO2002034784A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The nucleic acid and amino acid sequences of α1, α2, β and ε subunits of acetyl-CoA carboxylase (ACCase) from Streptomyces coelicolor are provided. This subunit structure differs from that of known acyl carboxylases. Materials and methods are provided of increasing ACCase activity and production of secondary metabolites (such as polyketides and especially antibiotics) by causing expression in Streptomyces of such nucleic acid. Also provided are methods of increasing ACCase activity and production of secondary metabolites (such as polyketides and especially antibiotics) by culturing Streptomyces in the presence of exogenous malonate.
PCT/GB2001/004709 2000-10-23 2001-10-23 An acyl coenzyme a carboxylase from streptomyces Ceased WO2002034784A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001295782A AU2001295782A1 (en) 2000-10-23 2001-10-23 An acyl coenzyme a carboxylase from streptomyces
EP01976514A EP1328551A2 (en) 2000-10-23 2001-10-23 An acyl coenzyme a carboxylase from streptomyces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002322105A CA2322105A1 (en) 2000-10-23 2000-10-23 Antibiotic production (ii)
CA2,322,105 2000-10-23

Publications (2)

Publication Number Publication Date
WO2002034784A2 WO2002034784A2 (en) 2002-05-02
WO2002034784A3 true WO2002034784A3 (en) 2003-01-16

Family

ID=4167299

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004709 Ceased WO2002034784A2 (en) 2000-10-23 2001-10-23 An acyl coenzyme a carboxylase from streptomyces

Country Status (4)

Country Link
EP (1) EP1328551A2 (en)
AU (1) AU2001295782A1 (en)
CA (1) CA2322105A1 (en)
WO (1) WO2002034784A2 (en)

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* Cited by examiner, † Cited by third party
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CN102695799A (en) 2009-09-27 2012-09-26 Opx生物工艺学公司 Method for producing 3-hydroxypropionic acid and other products
MX2012008700A (en) * 2010-01-27 2012-10-05 Univ Colorado Regents Microorganism production of high-value chemical products, and related compositions, methods and systems.
AU2013299414A1 (en) 2012-08-10 2015-03-26 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
CA2905602A1 (en) 2013-03-15 2014-09-18 Sarah M. Hoyt Flash evaporation for product purification and recovery
BR112016001026A2 (en) 2013-07-19 2017-10-24 Cargill Inc genetically modified organism
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN110494566A (en) 2017-02-02 2019-11-22 嘉吉公司 Generate the genetically modified cell of C6-C10 derivative of fatty acid
CN112410274B (en) * 2019-08-23 2023-01-24 上海医药工业研究院 Genetic engineering bacterium for producing ascomycin and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991010739A1 (en) * 1990-01-18 1991-07-25 Cetus Corporation Improved streptomyces promoter
WO1996000282A1 (en) * 1994-06-23 1996-01-04 Merck & Co., Inc. Expression cassettes useful in construction of integrative and replicative expression vectors for streptomyces
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991010739A1 (en) * 1990-01-18 1991-07-25 Cetus Corporation Improved streptomyces promoter
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
WO1996000282A1 (en) * 1994-06-23 1996-01-04 Merck & Co., Inc. Expression cassettes useful in construction of integrative and replicative expression vectors for streptomyces

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 24 July 1998 (1998-07-24), REDENBACH ET AL.: "A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome", XP002195193 *
DATABASE TREMBL 4 May 1999 (1999-05-04), RODRIGUEZ AND GRAMAJO: "Streptomyces coelicolor putative acyl-CoA carboxylase complex A subunit (accA1) gene, complete cds", XP002195260 *
LOMBO F ET AL: "Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering.", BIOTECHNOLOGY PROGRESS, (2001 JUL-AUG) 17 (4) 612-7., XP001087868 *
REDENBACH M ET AL: "A SET OF ORDERED COSMIDS AND A DETAILED GENETIC AND PHYSICAL MAP FOR THE 8 MB STREPTOMYCES COELICOLOR A3(2) CHROMOSOME", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 21, no. 1, 1996, pages 77 - 96, XP002932565, ISSN: 0950-382X *
RODRIGUEZ E ET AL: "Genetic and biochemical characterization of the alpha and beta components of a propionyl-CoA carboxylase complex of Streptomyces coelicolor A3(2).", MICROBIOLOGY (READING), vol. 145, no. 11, November 1999 (1999-11-01), pages 3109 - 3119, XP002195191, ISSN: 1350-0872 *
RODRIGUEZ E ET AL: "Role of an essential acyl coenzyme A carboxylase in the primary and secondary metabolism of Streptomyces coelicolor A3(2).", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 9, September 2001 (2001-09-01), pages 4166 - 4176, XP002195192, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
WO2002034784A2 (en) 2002-05-02
EP1328551A2 (en) 2003-07-23
AU2001295782A1 (en) 2002-05-06
CA2322105A1 (en) 2002-04-23

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