[go: up one dir, main page]

WO2002012564A3 - Methodes d'identification de polynucleotides peu abondants et compositions correspondantes - Google Patents

Methodes d'identification de polynucleotides peu abondants et compositions correspondantes Download PDF

Info

Publication number
WO2002012564A3
WO2002012564A3 PCT/US2001/024730 US0124730W WO0212564A3 WO 2002012564 A3 WO2002012564 A3 WO 2002012564A3 US 0124730 W US0124730 W US 0124730W WO 0212564 A3 WO0212564 A3 WO 0212564A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides
methods
polynucleotide sample
test
related compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/024730
Other languages
English (en)
Other versions
WO2002012564A2 (fr
Inventor
York Yuan-Yuan Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genemed Biotechnologies Inc
Original Assignee
Genemed Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/632,898 external-priority patent/US6465219B1/en
Application filed by Genemed Biotechnologies Inc filed Critical Genemed Biotechnologies Inc
Priority to US10/343,345 priority Critical patent/US20040006033A1/en
Priority to JP2002517847A priority patent/JP2004512027A/ja
Priority to EP01961937A priority patent/EP1395678A2/fr
Priority to AU2001283159A priority patent/AU2001283159A1/en
Publication of WO2002012564A2 publication Critical patent/WO2002012564A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002012564A3 publication Critical patent/WO2002012564A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne de nouvelles méthodes de production de plusieurs polynucléotides préparés à partir d'un échantillon de polynucléotides et la pluralité de polynucléotides ainsi produits. Dans un mode de réalisation, on prépare la pluralité de polynucléotides par hybridation soustractive réalisée entre des échantillons de polynucléotides de référence et d'essai, et on enrichit ladite pluralité de polynucléotides en séquences qui ne sont pas dans l'échantillon de polynucléotides de référence ou le sont dans une concentration inférieure à celle de l'échantillon de polynucléotides d'essai. Lesdits polynucléotides sont également enrichis en séquences peu abondantes, par rapport à l'échantillon de polynucléotides d'essai. Cette invention concerne aussi des kits utilisés dans les méthodes de cette même invention et destinés à l'utilisation des polynucléotides ainsi produits. On utilise les polynucléotides dans un grand nombre d'applications diverses, telles que le clonage, l'expression et les études portant sur l'hybridation.
PCT/US2001/024730 2000-08-07 2001-08-06 Methodes d'identification de polynucleotides peu abondants et compositions correspondantes Ceased WO2002012564A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/343,345 US20040006033A1 (en) 2001-08-06 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions
JP2002517847A JP2004512027A (ja) 2000-08-07 2001-08-06 低存在量ポリヌクレオチド及び関連する組成物の同定方法
EP01961937A EP1395678A2 (fr) 2000-08-07 2001-08-06 Methodes d'identification de polynucleotides peu abondants et compositions correspondantes
AU2001283159A AU2001283159A1 (en) 2000-08-07 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/632,898 US6465219B1 (en) 2000-08-07 2000-08-07 Polynucleotide pools enriched in either high-abundance or low-abundance sequences
US09/632,898 2000-08-07
US28877701P 2001-05-04 2001-05-04
US60/288,777 2001-05-04

Publications (2)

Publication Number Publication Date
WO2002012564A2 WO2002012564A2 (fr) 2002-02-14
WO2002012564A3 true WO2002012564A3 (fr) 2003-12-24

Family

ID=26965245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/024730 Ceased WO2002012564A2 (fr) 2000-08-07 2001-08-06 Methodes d'identification de polynucleotides peu abondants et compositions correspondantes

Country Status (4)

Country Link
EP (1) EP1395678A2 (fr)
JP (1) JP2004512027A (fr)
AU (1) AU2001283159A1 (fr)
WO (1) WO2002012564A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017044574A1 (fr) * 2015-09-11 2017-03-16 Cellular Research, Inc. Procédés et compositions pour la normalisation de banques d'acides nucléiques

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
WO1998042871A1 (fr) * 1997-03-24 1998-10-01 Boehringer Mannheim Corporation Procede d'hybridation soustractive et analyse differentielle
WO1999015702A1 (fr) * 1997-09-24 1999-04-01 Life Technologies, Inc. Banques normalisees d'acides nucleiques et leurs procede de production

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
WO1998042871A1 (fr) * 1997-03-24 1998-10-01 Boehringer Mannheim Corporation Procede d'hybridation soustractive et analyse differentielle
WO1999015702A1 (fr) * 1997-09-24 1999-04-01 Life Technologies, Inc. Banques normalisees d'acides nucleiques et leurs procede de production

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DIATCHENKO L ET AL: "SUPPRESSION SUBTRACTIVE HYBRIDIZATION: A METHOD FOR GENERATING DIFFERENTIALLY REGULATED OR TISSUE-SPECIFIC CDNA PROBES AND LIBRARIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, 1 June 1996 (1996-06-01), pages 6025 - 6030, XP002911922, ISSN: 0027-8424 *
DUGUID J R ET AL: "LIBRARY SUBTRACTION OF IN VITRO CDNA LIBRARIES TO IDENTIFY DIFFERENTIALLY EXPRESSED GENES IN SCRAPIE INFECTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 9, 11 May 1990 (1990-05-11), pages 2789 - 2792, XP000371880, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
EP1395678A2 (fr) 2004-03-10
WO2002012564A2 (fr) 2002-02-14
AU2001283159A1 (en) 2002-02-18
JP2004512027A (ja) 2004-04-22

Similar Documents

Publication Publication Date Title
WO2004070352A3 (fr) Procedes, melanges, kits et compositions relatives a la determination d'analytes
AU2002348563A1 (en) Nucleic acid enrichment
DE60220451D1 (de) Verfahren für die herstellung eines gewünschten profils von erythropoietin glyko-isoformen
CA2221032A1 (fr) Procede de preparation de produits melanges
WO2002031203A3 (fr) Criblage a haut rendement ou de type capillaire destine a identifier une bio-activite ou une biomolecule
DE60210406D1 (de) Erhöhung des durchsatzes in einem automatischen klinischen analysierer durch aufteilen von assays nach typ
DE69839668D1 (de) Ein in-vitro-verfahren zur herstellung einer dna-bibliothek
WO2004083427A3 (fr) Codage par ligature de petites molecules
GB2422196A (en) System for detecting polynucleotides
ES2045127T3 (es) Metodo de multiplicacion para ensayos de polinucleotidos.
HRP20080464T3 (en) Method for purifying fsh
DE602005017370D1 (de) Ligationscodierung unter verwendung von oligonukleotidbausteinen
DE69718054D1 (de) Verfahren zur aufreinigung von dns
DK1504098T4 (da) Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
DE69327059D1 (de) Verfahren zur herstellung von testelementen
WO2005004191A3 (fr) Substrat pour desorption laser
Merdinoglu et al. Comparison of RAPD, AFLP and SSR (microsatellite) markers for genetic diversity analysis in Vitis vinifera L.
EP1634962A4 (fr) Methode de detection d'une sequence nucleotidique cible, structure cible de detection a utiliser pour la mise en oeuvre de cette methode, procede de production de cette structure et trousse d'analyse destinee a detecter une sequence nucleotidique cible
Xu et al. Ecological shifts and hominin adaptations during the Mid-Pleistocene Climate Transition in Northeast Asia as evidenced by isotopic analysis (δ13C, δ18O) of mammalian enamel from Early Paleolithic sites in the Nihewan Basin, China
DE60332600D1 (de) Selektive elution von immobilisierten multiplexierten primerverlängerungsprodukten
WO2002012564A3 (fr) Methodes d'identification de polynucleotides peu abondants et compositions correspondantes
PT1368491E (pt) Processo para a selecção, por separação bidimensional, de ácidos nucleicos que se ligam com elevada afinidade a um alvo
IS5847A (is) Aðferð við að greina breytileika í fjölkirnaröð
EP1001963A4 (fr) Echelles d'acides nucleiques
WO2004042341A3 (fr) Procede pour acheminer des echantillons de suspension a des chambres a echantillons

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002517847

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2001961937

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 10343345

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2001961937

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001961937

Country of ref document: EP