[go: up one dir, main page]

WO2002012564A3 - Methods for identifying low-abundance polynucleotides and related compositions - Google Patents

Methods for identifying low-abundance polynucleotides and related compositions Download PDF

Info

Publication number
WO2002012564A3
WO2002012564A3 PCT/US2001/024730 US0124730W WO0212564A3 WO 2002012564 A3 WO2002012564 A3 WO 2002012564A3 US 0124730 W US0124730 W US 0124730W WO 0212564 A3 WO0212564 A3 WO 0212564A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides
methods
polynucleotide sample
test
related compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/024730
Other languages
French (fr)
Other versions
WO2002012564A2 (en
Inventor
York Yuan-Yuan Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genemed Biotechnologies Inc
Original Assignee
Genemed Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/632,898 external-priority patent/US6465219B1/en
Application filed by Genemed Biotechnologies Inc filed Critical Genemed Biotechnologies Inc
Priority to EP01961937A priority Critical patent/EP1395678A2/en
Priority to JP2002517847A priority patent/JP2004512027A/en
Priority to AU2001283159A priority patent/AU2001283159A1/en
Priority to US10/343,345 priority patent/US20040006033A1/en
Publication of WO2002012564A2 publication Critical patent/WO2002012564A2/en
Anticipated expiration legal-status Critical
Publication of WO2002012564A3 publication Critical patent/WO2002012564A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention provides novel methods for producing a plurality of polynucleotides prepared from a polynucleotide sample and the plurality of polynucleotides so produced. In one embodiment, the plurality of polynucleotides is prepared by subtractive hybridization between test and reference polynucleotide samples and is substantially enriched in sequences that are either not present in the reference polynucleotide sample or are present in the reference polynucleotide sample in substantially lower concentration than in the test polynucleotide sample. The plurality of polynucleotides is also substantially enriched in low-abundance sequences, relative to the test polynucleotide sample. The invention also provides kits useful in the methods of the invention and for using the polynucleotides produced thereby. The polynucleotides are useful in a wide variety of applications, such as cloning, expression, and hybridization studies.
PCT/US2001/024730 2000-08-07 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions Ceased WO2002012564A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01961937A EP1395678A2 (en) 2000-08-07 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions
JP2002517847A JP2004512027A (en) 2000-08-07 2001-08-06 Methods for identifying low abundance polynucleotides and related compositions
AU2001283159A AU2001283159A1 (en) 2000-08-07 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions
US10/343,345 US20040006033A1 (en) 2001-08-06 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/632,898 US6465219B1 (en) 2000-08-07 2000-08-07 Polynucleotide pools enriched in either high-abundance or low-abundance sequences
US09/632,898 2000-08-07
US28877701P 2001-05-04 2001-05-04
US60/288,777 2001-05-04

Publications (2)

Publication Number Publication Date
WO2002012564A2 WO2002012564A2 (en) 2002-02-14
WO2002012564A3 true WO2002012564A3 (en) 2003-12-24

Family

ID=26965245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/024730 Ceased WO2002012564A2 (en) 2000-08-07 2001-08-06 Methods for identifying low-abundance polynucleotides and related compositions

Country Status (4)

Country Link
EP (1) EP1395678A2 (en)
JP (1) JP2004512027A (en)
AU (1) AU2001283159A1 (en)
WO (1) WO2002012564A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108026524A (en) * 2015-09-11 2018-05-11 赛卢拉研究公司 Method and composition for nucleic acid library standardization

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
WO1998042871A1 (en) * 1997-03-24 1998-10-01 Boehringer Mannheim Corporation Procedure for subtractive hybridization and difference analysis
WO1999015702A1 (en) * 1997-09-24 1999-04-01 Life Technologies, Inc. Normalized nucleic acid libraries and methods of production thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
WO1998042871A1 (en) * 1997-03-24 1998-10-01 Boehringer Mannheim Corporation Procedure for subtractive hybridization and difference analysis
WO1999015702A1 (en) * 1997-09-24 1999-04-01 Life Technologies, Inc. Normalized nucleic acid libraries and methods of production thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DIATCHENKO L ET AL: "SUPPRESSION SUBTRACTIVE HYBRIDIZATION: A METHOD FOR GENERATING DIFFERENTIALLY REGULATED OR TISSUE-SPECIFIC CDNA PROBES AND LIBRARIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, 1 June 1996 (1996-06-01), pages 6025 - 6030, XP002911922, ISSN: 0027-8424 *
DUGUID J R ET AL: "LIBRARY SUBTRACTION OF IN VITRO CDNA LIBRARIES TO IDENTIFY DIFFERENTIALLY EXPRESSED GENES IN SCRAPIE INFECTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 9, 11 May 1990 (1990-05-11), pages 2789 - 2792, XP000371880, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
JP2004512027A (en) 2004-04-22
EP1395678A2 (en) 2004-03-10
WO2002012564A2 (en) 2002-02-14
AU2001283159A1 (en) 2002-02-18

Similar Documents

Publication Publication Date Title
ATE515702T1 (en) METHODS, MIXTURES, KITS AND COMPOSITIONS FOR ANALYTE DETERMINATION
DE60220451D1 (en) PROCESS FOR THE PREPARATION OF A DESIRED PROFILE OF ERYTHROPOIETIN GLYCOS ISOFORMS
WO2002031203A3 (en) High throughput or capillary-based screening for a bioactivity or biomolecule
EP0871976A4 (en) PROCESS FOR THE PREPARATION OF MIXED PRODUCTS
CA2225363A1 (en) Method for predicting chemical or physical properties of complex mixtures
DE60210406D1 (en) INCREASING THE THROUGHPUT IN AN AUTOMATIC CLINICAL ANALYZER BY SHARING ASSAYS BY TYPE
DE69839668D1 (en) An In Vitro Method for Making a DNA Library
WO2004083427A3 (en) Ligational encoding of small molecules
GB2422196A (en) System for detecting polynucleotides
ES2045127T3 (en) MULTIPLICATION METHOD FOR TESTING OF POLYNUCLEOTIDES.
HRP20080464T3 (en) Method for purifying fsh
WO2004003220A3 (en) Methods and composition for analyzing compromised samples using snps
DE602005017370D1 (en) LIGATION CODING USING OLIGONUCLEOTIDE COMPONENTS
ATE230034T1 (en) METHOD FOR PURIFYING DNA
DK1504098T4 (en) Process for molecular evolution in vitro of protein function
ATE186781T1 (en) METHOD FOR PRODUCING TEST ELEMENTS
WO2005004191A3 (en) Laser desorption substrate
WO2002086155A3 (en) Method for the preferential nucleic acid amplification of selected nucleic acid regions
Merdinoglu et al. Comparison of RAPD, AFLP and SSR (microsatellite) markers for genetic diversity analysis in Vitis vinifera L.
EP1634962A4 (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence
Xu et al. Ecological shifts and hominin adaptations during the Mid-Pleistocene Climate Transition in Northeast Asia as evidenced by isotopic analysis (δ13C, δ18O) of mammalian enamel from Early Paleolithic sites in the Nihewan Basin, China
WO2002012564A3 (en) Methods for identifying low-abundance polynucleotides and related compositions
IL141329A0 (en) Method for determining polynucleotide sequence variations
PT1368491E (en) Method of selection, by two-dimensional separation, of nucleic acids that bind to a target with high affinity
EP1001963A4 (en) Nucleic acid ladders

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002517847

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2001961937

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 10343345

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2001961937

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001961937

Country of ref document: EP