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WO2002011889A1 - Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre - Google Patents

Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre Download PDF

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Publication number
WO2002011889A1
WO2002011889A1 PCT/GB2001/003471 GB0103471W WO0211889A1 WO 2002011889 A1 WO2002011889 A1 WO 2002011889A1 GB 0103471 W GB0103471 W GB 0103471W WO 0211889 A1 WO0211889 A1 WO 0211889A1
Authority
WO
WIPO (PCT)
Prior art keywords
printing
printhead
liquids
liquid
different liquids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/003471
Other languages
English (en)
Inventor
Howard John Manning
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arrayjet Ltd
Original Assignee
Arrayjet Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arrayjet Ltd filed Critical Arrayjet Ltd
Priority to ES01954138T priority Critical patent/ES2256275T3/es
Priority to DE60116326T priority patent/DE60116326T2/de
Priority to EP01954138A priority patent/EP1307292B1/fr
Priority to AU2001276486A priority patent/AU2001276486B2/en
Priority to JP2002517214A priority patent/JP4833495B2/ja
Priority to AT01954138T priority patent/ATE314148T1/de
Priority to CA002414457A priority patent/CA2414457A1/fr
Priority to AU7648601A priority patent/AU7648601A/xx
Priority to KR10-2003-7001487A priority patent/KR20030045780A/ko
Publication of WO2002011889A1 publication Critical patent/WO2002011889A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J2/00—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed
    • B41J2/005—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
    • B41J2/01—Ink jet
    • B41J2/135—Nozzles
    • B41J2/14—Structure thereof only for on-demand ink jet heads
    • B41J2/14201—Structure of print heads with piezoelectric elements
    • B41J2/14209—Structure of print heads with piezoelectric elements of finger type, chamber walls consisting integrally of piezoelectric material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J3/00—Typewriters or selective printing or marking mechanisms characterised by the purpose for which they are constructed
    • B41J3/407—Typewriters or selective printing or marking mechanisms characterised by the purpose for which they are constructed for marking on special material
    • B41J3/4073—Printing on three-dimensional objects not being in sheet or web form, e.g. spherical or cubic objects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00385—Printing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41J—TYPEWRITERS; SELECTIVE PRINTING MECHANISMS, i.e. MECHANISMS PRINTING OTHERWISE THAN FROM A FORME; CORRECTION OF TYPOGRAPHICAL ERRORS
    • B41J2/00—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed
    • B41J2/005—Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
    • B41J2/01—Ink jet
    • B41J2/135—Nozzles
    • B41J2/14—Structure thereof only for on-demand ink jet heads
    • B41J2002/14419—Manifold
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1041—Ink-jet like dispensers

Definitions

  • This invention relates to the use of ink jet printers to make biological microarrays.
  • microarray also known as the DNA microarray or biochip, and by other terminology.
  • This consists of a substrate on which a compact array of biological or chemical samples, known as probes, is immobilised.
  • the microarray is exposed to a sample, known as the target, which is to be tested against the probes.
  • the interactions are recorded by suitable instrumentation and the data is manipulated.
  • Microarrays are made at present by two methods: the probes can be synthesised on the array, by applying constituents of the probes to build them up in situ; or pre-synthesised probes can be spotted onto the array. This invention relates to the latter method.
  • the task of spotting a microarray consists of transferring extremely small amounts of many different liquids from separate reservoirs to closely spaced positions on a number of microarrays. There may be anything from tens of different liquids to hundreds of thousands of them, supplied typically in multiple 96, 384 or 1536-well microtitre plates. Some tens or hundreds of substrates need to be spotted with each of the liquids; typical spot volumes are of the order of a nanlolitre, and spots may be separated by a few hundred microns.
  • Spotting is achieved at present in two main ways: in the first method, pins are dipped into the wells to pick up samples of the liquids, and then moved on a three-axis transport to touch the substrates and deposit drops. Several pins may be used in parallel to speed up the spotting.
  • the pins have to be washed and dried before picking up samples of another set of liquids.
  • the pins have to touch the substrate, which requires high precision, carries a risk of damage, and is slow.
  • the volume of liquid spotted is rather large, is not well controlled and cannot be varied easily.
  • the configuration of spots on the microarray corresponds to the arrangement of liquids in the wells, as the pins are all brought into contact with the • substrate simultaneously. A considerable proportion of each liquid is wasted.
  • the second method of spotting is to project the liquid through the air onto the substrate, without contact.
  • ink jet printing technology is eminently suitable: it produces small droplets, very reproducibly, and positions them accurately on the substrate. In some cases, the droplets are sufficiently small that multiple droplets can be applied to a given spot to vary its volume. Ink jet printing is very rapid, and is entirely flexible as to what liquid is deposited where on the substrate. * The main difficulty with ink jet technology is that, although some printheads have large numbers of nozzles, they are designed to print typically one or four colours of ink. Their inlets lead to manifolds which connect many chambers, each associated with a nozzle.
  • the present invention relates to a way of using standard ink jet printheads to handle a number of different liquids larger than the number of colours it is designed to print, without a mixture of the liquids being printed.
  • printing apparatus capable of printing a number of different samples without the samples being mixed, wherein the printing apparatus comprises a printhead which itself comprises one or more manifolds, and wherein the number of samples that may be printed is larger than the number of manifolds.
  • a manifold is connected to more than one chamber.
  • each chamber is associated with one or more nozzle (s) .
  • nozzle Preferably there are a number of nozzles which are formed in a nozzle plate.
  • nozzles are used for each sample. This allows multiple drops to be placed on a given spot on a microarray in a single pass. It also extends the useful lifetime of the printhead against the possibility of blockage of nozzles by dirt, or other failure, by ensuring that alternative nozzles are available to print a given sample.
  • each chamber is longer in the direction of liquid motion during printing than in a perpendicular direction to the direction of liquid motion during printing.
  • each nozzle is capable of ejecting drops of sample.
  • sample liquid is introduced via the nozzles.
  • each nozzle acts as a restrictor to control the rate of introduction of liquid into the chamber.
  • the printhead is full of fluid at the outset.
  • the fluid is a liquid.
  • the printhead is full of a solid at the outset.
  • the solid is a weak solid which has deformable properties.
  • the printing apparatus comprises a connection block attached to the nozzle plate.
  • connection block comprises seals which act against the printhead nozzles to separate different liquids.
  • connection block may have a layered structure.
  • connection block comprises a filter layer to prevent dirt from entering the printhead.
  • the samples are held in wells on a plate (e.g., a microtitre plate) prior to being taken into the printhead.
  • a plate e.g., a microtitre plate
  • the printhead is attached to a moving means which allows the sample to be picked up at one point and expelled at a second point.
  • sample is printed over a range of positions.
  • the printhead is stationary and the substrate on which printing is to occur will move relative to the printhead.
  • the printhead is positioned so that the line of nozzles is parallel to the direction of motion during printing.
  • the printhead is positioned so that the nozzles run perpendicular to the direction of printing.
  • the printhead is angled in order to alter the effective resolution perpendicular to the motion.
  • a number of different liquids larger than the number of manifolds is introduced into the chambers via the nozzles.
  • the volume of the liquid printed from each nozzle is less than the volume of the chamber associated with that nozzle, in order to ensure that the liquid printed is uncontaminated by the mixture of liquids likely to be present in the manifolds.
  • the volume of the liquid printed from each nozzle is less than the volume of liquid introduced into the chamber, again in order to avoid contamination.
  • the volume of liquid introduced into each nozzle is greater than the volume of the chamber associated with that nozzle, in order to maximise the volume of liquid which can be printed without contamination.
  • printing is carried out within a time after the introduction of the different liquids less than the time taken for diffusion to contaminate the liquid in any chamber with liquid from any other chamber via the manifold connecting them.
  • the liquids introduced- via the nozzles displace the initial liquid towards and into the manifolds.
  • the liquids are introduced into the nozzles by the application of suction to the manifolds.
  • the nozzles act as restrictors to control the rate of introduction of liquid.
  • the liquids are introduced into the nozzles by the application of pressure in the sample wells so that the liquids are forced in.
  • sample wells are provided with a penetrable seal.
  • the wells can each be pressurised separately and different pressures can be applied to each well.
  • the wells may be pressurised using pistons.
  • a further alternative is that the liquids are introduced into the nozzles by the actuation in reverse of the printhead.
  • the volume of each liquid printed is a high proportion of the volume introduced into the printhead.
  • the total quantity of liquid printed from each nozzle may be increased above the volume of the associated chamber by:
  • the printhead is cleaned by the introduction of neutral liquid into the printhead either via the nozzles or via the manifolds.
  • a commercially promising application of the invention is to print the liquids as spots of picolitre to nanolitre volume onto a substrate for the production of biological or chemical microarrays.
  • An advantage of ink jet printheads is that they can print while there is relative motion between the printhead and the substrate, increasing speed of production.
  • Figure 1 shows a side cross-sectional view of a suitable ink jet printhead
  • Figure 2 shows a front cross-sectional view of a suitable ink jet printhead
  • Figure 3 shows the liquids being introduced into the printhead from a microtitre plate by means of a connection block.
  • each nozzle 1 is associated with a long narrow chamber 4 excavated within lower component 3.
  • the chambers open out at the rear into manifolds 5 each serving multiple chambers.
  • manifolds 5 There are typically one or four manifolds 5, each fed by a supply via a filter 6.
  • Drops are ejected when the walls between the chambers 4 are deflected, causing pressure waves within the chambers 4.
  • the length of the chambers 4, defined by cut-outs in the upper component 2, determines the drop size ejected. If the chambers 4 are long (6mm in a particular printhead) , relatively large drops (typically 50 picolitres) are ejected, which are suitable for binary printing and also for the production of microarrays. If the chambers 4 are short (1 mm in another printhead) , small drops (typically 7 picolitres) are produced; then multiple drops can be used for greyscale printing, or for producing microarray spots of controllable size.
  • the chambers 4 are long compared with their lateral dimensions (typically 75 microns by 390 microns), so the liquid tends to advance along the chambers 4 towards the nozzles 1 as printing proceeds; there is little tendency for liquid at the rear of a chamber 4, or that entering the chamber 4 from the manifold 5, to mix with liquid near the nozzle 1.
  • the pathway for diffusion to introduce into a chamber 4 liquid from another chamber 4, via the connecting manifold 5, is long and unfavourable for mixing. Therefore nearly the entire contents of a chamber 4 can be printed without contamination by liquid from elsewhere.
  • connection block 10 interposed between the printhead 9 and a microtitre plate 11 to allow multiple liquids to be introduced into the printhead via the nozzles 1.
  • connection block 10 would include a filter layer.
  • connection block 10 has moulded rubber seals 12 which separate multiple regions 13 (typically 48 in number) of the printhead, each containing several nozzles 1 (typically seven, with three blocked by the seal 12).
  • Capillaries 14 project downwards from the regions 13 into the wells 15 of a microtitre plate.
  • the pitch of the wells 15 (typically 4.5mm for a 384-well plate) is larger than that of the regions 13 (typically ten times the pitch 141 microns of the nozzles) , so there need to be multiple (typically three) rows of capillaries 14; only one row is shown in Figure 3.
  • the printhead 9 may initially be full of a neutral liquid. Suction is applied at point 8 until samples have been drawn into the printhead 9, slightly more than filling the corresponding chambers.
  • the nozzles may act as restriotors to control the flowrate during filling. If the nozzles are of small diameter at their exit faces than internally, they resist ingress of any dirt particles sufficiently large to block nozzles subsequently.
  • the connection block 10 can be equipped with a course filter to minimise the population of dirt particles entering the printhead 9.
  • An alternative embodiment would have the sample being forced into nozzles 1 from the wells 15. This could be done by pressurising the wells 1, which may be provided with self-sealing covers, so that the liquid is pushed out of them into the nozzles 1; alternatively seals on the lower surface of the connection block could isolate the wells. Sealing the wells 1 would have the benefit that different wells 1 could be placed under different pressures so that different amounts of sample could be pushed into the nozzle 1; the seals could also guard against dirt from the atmosphere getting into the printhead. Another way in which samples could be forced into the nozzles 1 is by placing the samples in a pre- loaded cartridge comprising of reservoirs equipped with pistons which push samples out of the reservoirs when required.
  • the liquids As soon as the liquids have been introduced into the printhead 9, it is detached from the connection block 10, wiped and moved by means of an x-y-z motion control to the microarrays to be spotted.
  • the amount of liquid printed from each nozzle 1 is less than the volume of the corresponding chamber 4, so the mixture of liquids in the manifolds 5 is not printed.
  • the timescale of the printing (seconds) does not allow diffusion to contaminate one chamber 4 with the liquid from another.
  • the row of nozzles 1 is parallel to the direction of relative motion during printing, as this would allow multiple drops to be placed at one point, increasing the amount of liquid at that point.
  • the fraction of the liquid drawn from the wells 15 which is wasted should be substantially less than half.
  • the printhead 9 may be taken to a filling station and neutral liquid drawn in through the nozzles. Then another set of liquids can be charged into the printhead 9 and spotted.
  • neutral liquid prevents contamination of the liquid in a chamber 4 by residues of liquids previously introduced into it. Perfect displacement of the liquid in a chamber 4 by neutral liquid entering via its nozzle 1 is impossible, so the volume of neutral liquid introduced into each nozzle 1 should be several times the volume of the chamber 4 associated with that nozzle 1.
  • the next set of liquids is introduced, they will be diluted slightly by the neutral liquid present in each chamber 4, however, the dilution will be very small and consistent.
  • the time taken to spot a complex microarray by conventional means is dominated by the speed of the x-y-z motion and the loading of the printhead.
  • Ink jet printers are capable of printing while the printhead is in motion, or the substrate is moving relative to the printhead; and the present invention allows the printhead to be loaded with multiple liquids without emptying and drying the printhead.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Manufacturing & Machinery (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Coating Apparatus (AREA)
  • Particle Formation And Scattering Control In Inkjet Printers (AREA)
  • Ink Jet (AREA)

Abstract

L'invention concerne un procédé de fonctionnement d'une tête d'impression à jet d'encre pourvue d'au moins un collecteur relié à plus d'une chambre, chaque chambre étant associée à une buse et pouvant éjecter des gouttes. Un certain nombre de différents liquides plus importants que le nombre de collecteurs est introduit dans la tête d'impression par le biais des buses, et le volume du liquide ensuite imprimé à partir de chaque buse est inférieur au volume de la chambre associée à cette buse, ainsi qu'au volume de liquide introduit dans cette chambre. A l'aide de ce dispositif, la tête d'impression imprime plus de liquides différents que cela n'est possible traditionnellement et sans les mélanger.
PCT/GB2001/003471 2000-08-03 2001-08-02 Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre Ceased WO2002011889A1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
ES01954138T ES2256275T3 (es) 2000-08-03 2001-08-02 Fabricacion extremadamente en paralelo de micromatrices mediante cabezas de impresion por chorro de tinta.
DE60116326T DE60116326T2 (de) 2000-08-03 2001-08-02 Hochparallele herstellung von mikroarrays mittels tintenstrahldruckkopf
EP01954138A EP1307292B1 (fr) 2000-08-03 2001-08-02 Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre
AU2001276486A AU2001276486B2 (en) 2000-08-03 2001-08-02 Highly parallel fabrication of microarrays by ink jet printheads
JP2002517214A JP4833495B2 (ja) 2000-08-03 2001-08-02 インクジェットプリントヘッドによるマイクロアレイの高度平行構成体
AT01954138T ATE314148T1 (de) 2000-08-03 2001-08-02 Hochparallele herstellung von mikroarrays mittels tintenstrahldruckkopf
CA002414457A CA2414457A1 (fr) 2000-08-03 2001-08-02 Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre
AU7648601A AU7648601A (en) 2000-08-03 2001-08-02 Highly parallel fabrication of microarrays by ink jet printheads
KR10-2003-7001487A KR20030045780A (ko) 2000-08-03 2001-08-02 잉크 젯 프린트헤드에 의한 고도의 병렬 마이크로어레이의제조

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0018963.9 2000-08-03
GBGB0018963.9A GB0018963D0 (en) 2000-08-03 2000-08-03 Highly parallel fabrication of microarrays by ink jet printheads
US10/350,776 US7128393B2 (en) 2000-08-03 2003-01-24 Highly parallel fabrication of microarrays by ink jet printheads

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10473727 A-371-Of-International 2001-08-21
US11/788,888 Division US7598386B2 (en) 2001-04-05 2007-04-23 Sulphones which modulate the action of gamma-secretase

Publications (1)

Publication Number Publication Date
WO2002011889A1 true WO2002011889A1 (fr) 2002-02-14

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PCT/GB2001/003471 Ceased WO2002011889A1 (fr) 2000-08-03 2001-08-02 Fabrication hautement parallele de micro-matrices au moyen de tetes d'impression a jet d'encre

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AT (1) ATE314148T1 (fr)
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WO2004028683A1 (fr) 2002-09-27 2004-04-08 Arrayjet Limited Procede et appareil de manipulation et d'impression de substrat
WO2004073988A3 (fr) * 2003-02-20 2004-10-28 Arrayjet Ltd Procede et appareil d'impression perfectionnes
GB2404191A (en) * 2003-05-30 2005-01-26 Agilent Technologies Inc Pulse jet ejection head diagnostic system
CN108993625A (zh) * 2018-08-28 2018-12-14 北京迈克诺科技有限公司 基于喷墨打印技术的压电式点样装置
WO2020208377A1 (fr) 2019-04-11 2020-10-15 Arrayjet Limited Procédé et appareil pour la manipulation et l'impression de substrats

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DE102011001550A1 (de) * 2011-03-25 2012-09-27 Friz Biochem Gesellschaft Für Bioanalytik Mbh Vorrichtung zum Fördern und Mischen von Mikromengen an Reagenzien und zur Durchführung chemischer Reaktionen
US11768215B2 (en) * 2019-01-04 2023-09-26 Funai Electric Co., Ltd. Digital dispense system cartridge
CN118179371A (zh) * 2024-02-22 2024-06-14 北京擎科生物科技股份有限公司 寡核苷酸合成系统及控制方法

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WO2000051736A1 (fr) * 1999-03-04 2000-09-08 Ut-Battelle, Llc. Systeme a collecteur double et procede de transfert de fluide en parallele

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US5658802A (en) * 1995-09-07 1997-08-19 Microfab Technologies, Inc. Method and apparatus for making miniaturized diagnostic arrays
US6083763A (en) * 1996-12-31 2000-07-04 Genometrix Inc. Multiplexed molecular analysis apparatus and method
WO1998045205A2 (fr) * 1997-04-08 1998-10-15 Packard Instrument Company, Inc. Systeme pour la manipulation de microvolumes de liquides
WO2000051736A1 (fr) * 1999-03-04 2000-09-08 Ut-Battelle, Llc. Systeme a collecteur double et procede de transfert de fluide en parallele

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004028683A1 (fr) 2002-09-27 2004-04-08 Arrayjet Limited Procede et appareil de manipulation et d'impression de substrat
WO2004073988A3 (fr) * 2003-02-20 2004-10-28 Arrayjet Ltd Procede et appareil d'impression perfectionnes
GB2404191A (en) * 2003-05-30 2005-01-26 Agilent Technologies Inc Pulse jet ejection head diagnostic system
GB2404191B (en) * 2003-05-30 2007-10-31 Agilent Technologies Inc Pulse jet ejection head diagnostic system
CN108993625A (zh) * 2018-08-28 2018-12-14 北京迈克诺科技有限公司 基于喷墨打印技术的压电式点样装置
WO2020208377A1 (fr) 2019-04-11 2020-10-15 Arrayjet Limited Procédé et appareil pour la manipulation et l'impression de substrats

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GB0018963D0 (en) 2000-09-20
DE60116326D1 (de) 2006-02-02
AU7648601A (en) 2002-02-18
ATE314148T1 (de) 2006-01-15
US7128393B2 (en) 2006-10-31
AU2001276486B2 (en) 2006-07-27
ES2256275T3 (es) 2006-07-16
EP1307292A1 (fr) 2003-05-07
DE60116326T2 (de) 2006-07-13
US20040145631A1 (en) 2004-07-29
CA2414457A1 (fr) 2002-02-14
EP1307292B1 (fr) 2005-12-28

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