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WO2002065092A3 - Dispositifs et procedes destines a des tests patch clamp a haut debit - Google Patents

Dispositifs et procedes destines a des tests patch clamp a haut debit Download PDF

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Publication number
WO2002065092A3
WO2002065092A3 PCT/US2002/003700 US0203700W WO02065092A3 WO 2002065092 A3 WO2002065092 A3 WO 2002065092A3 US 0203700 W US0203700 W US 0203700W WO 02065092 A3 WO02065092 A3 WO 02065092A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
devices
plate
high throughput
patch clamp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/003700
Other languages
English (en)
Other versions
WO2002065092A9 (fr
WO2002065092A2 (fr
Inventor
Andrew J G Kelly
Haim Kedar
Kerwin Ng
Larry Costas Mattheakis
Alexei Nikolai Savtchenko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affymax Inc
Original Assignee
Affymax Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/779,955 external-priority patent/US20020108869A1/en
Application filed by Affymax Inc filed Critical Affymax Inc
Priority to JP2002564562A priority Critical patent/JP2004528539A/ja
Priority to EP02707739A priority patent/EP1358474A2/fr
Priority to AU2002242128A priority patent/AU2002242128A1/en
Publication of WO2002065092A2 publication Critical patent/WO2002065092A2/fr
Publication of WO2002065092A9 publication Critical patent/WO2002065092A9/fr
Publication of WO2002065092A3 publication Critical patent/WO2002065092A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif destiné à mesurer les propriétés électrophysiologiques d'une membrane cellulaire d'une cellule individuelle comprenant une plaque comportant au moins un orifice. L'orifice est limité par une surface et cette surface est modifiée, notamment via un traitement thermique, afin de faciliter la formation d'un scellement à résistance très élevée (gigaohm). Une chambre est située adjacente à la plaque. La chambre est en communication libre avec au moins une orifice et elle est conçue pour contenir une solution conduisant l'électricité. La plaque comprend, en outre, une première électrode disposée dans la chambre et une seconde électrode disposée adjacente à la plaque.
PCT/US2002/003700 2001-02-09 2002-02-08 Dispositifs et procedes destines a des tests patch clamp a haut debit Ceased WO2002065092A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2002564562A JP2004528539A (ja) 2001-02-09 2002-02-08 高スループットパッチクランプアッセイのための装置および方法
EP02707739A EP1358474A2 (fr) 2001-02-09 2002-02-08 Dispositifs et procedes destines a des tests patch clamp a haut debit
AU2002242128A AU2002242128A1 (en) 2001-02-09 2002-02-08 Devices and methods for high throughput patch clamp assays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/779,955 US20020108869A1 (en) 2001-02-09 2001-02-09 Device and technique for multiple channel patch clamp recordings
US09/779,955 2001-02-09
US10/029,425 US20020164777A1 (en) 2001-02-09 2001-12-21 Devices and methods for high throughput patch clamp assays
US10/029,425 2001-12-21

Publications (3)

Publication Number Publication Date
WO2002065092A2 WO2002065092A2 (fr) 2002-08-22
WO2002065092A9 WO2002065092A9 (fr) 2002-11-28
WO2002065092A3 true WO2002065092A3 (fr) 2003-02-20

Family

ID=26704933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003700 Ceased WO2002065092A2 (fr) 2001-02-09 2002-02-08 Dispositifs et procedes destines a des tests patch clamp a haut debit

Country Status (3)

Country Link
EP (1) EP1358474A2 (fr)
JP (1) JP2004528539A (fr)
WO (1) WO2002065092A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
EP1372828A4 (fr) 2001-03-24 2008-10-29 Aviva Biosciences Corp Biopuces comprenant des structures de detection de transport d'ions et procedes d'utilisation correspondants
AU2003231300A1 (en) 2002-05-04 2003-11-17 Aviva Biosciences Corporation Apparatus including ion transport detecting structures and methods of use
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
JP4552423B2 (ja) 2003-11-21 2010-09-29 パナソニック株式会社 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法
JP4691407B2 (ja) * 2005-06-29 2011-06-01 パナソニック株式会社 細胞電位測定用容器
JP4834335B2 (ja) * 2005-06-29 2011-12-14 パナソニック株式会社 細胞電位測定用容器
EP1801586B1 (fr) * 2002-08-21 2010-10-13 Cellectricon Ab Système pour obtenir des joints conservant une grande résistance dans des enregistrements patch-clamp
JP4386835B2 (ja) * 2002-08-21 2009-12-16 セレクトリコン アーベー パッチクランプ記録において高抵抗固着を獲得して維持するシステム及び方法
JP4661539B2 (ja) * 2005-11-11 2011-03-30 パナソニック株式会社 細胞電気生理センサおよびその製造方法
US8329009B2 (en) * 2010-04-09 2012-12-11 Molecular Devices, Llc High throughput screening of ion channels

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4324255A (en) * 1980-03-07 1982-04-13 Barach John P Method and apparatus for measuring magnetic fields and electrical currents in biological and other systems
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
US6071702A (en) * 1998-09-04 2000-06-06 Hitachi Software Engineering Co., Ltd. Probe-bearing element and method for producing the same
US20020064841A1 (en) * 2000-02-11 2002-05-30 Klemic Kathryn G. Planar patch clamp electrodes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4324255A (en) * 1980-03-07 1982-04-13 Barach John P Method and apparatus for measuring magnetic fields and electrical currents in biological and other systems
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
US6071702A (en) * 1998-09-04 2000-06-06 Hitachi Software Engineering Co., Ltd. Probe-bearing element and method for producing the same
US20020064841A1 (en) * 2000-02-11 2002-05-30 Klemic Kathryn G. Planar patch clamp electrodes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ALMERS ET AL.: "Slow calcium and potassium currents across frog muscle membrane: measurements with a vaseline-gap technique", J. PHYSIOL., vol. 312, 1981, pages 159 - 176, XP002959619 *
COSTA ET AL.: "Improved technique for studying ion channels expressed in xenopus oocytes, including fast superfusion", BIOPHYSICAL JOURNAL, vol. 67, July 1994 (1994-07-01), pages 395 - 401, XP002959618 *

Also Published As

Publication number Publication date
WO2002065092A9 (fr) 2002-11-28
EP1358474A2 (fr) 2003-11-05
WO2002065092A2 (fr) 2002-08-22
JP2004528539A (ja) 2004-09-16

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