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WO2002065092A3 - Devices and methods for high throughput patch clamp assays - Google Patents

Devices and methods for high throughput patch clamp assays Download PDF

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Publication number
WO2002065092A3
WO2002065092A3 PCT/US2002/003700 US0203700W WO02065092A3 WO 2002065092 A3 WO2002065092 A3 WO 2002065092A3 US 0203700 W US0203700 W US 0203700W WO 02065092 A3 WO02065092 A3 WO 02065092A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
devices
plate
high throughput
patch clamp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/003700
Other languages
French (fr)
Other versions
WO2002065092A9 (en
WO2002065092A2 (en
Inventor
Andrew J G Kelly
Haim Kedar
Kerwin Ng
Larry Costas Mattheakis
Alexei Nikolai Savtchenko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affymax Inc
Original Assignee
Affymax Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/779,955 external-priority patent/US20020108869A1/en
Application filed by Affymax Inc filed Critical Affymax Inc
Priority to JP2002564562A priority Critical patent/JP2004528539A/en
Priority to EP02707739A priority patent/EP1358474A2/en
Priority to AU2002242128A priority patent/AU2002242128A1/en
Publication of WO2002065092A2 publication Critical patent/WO2002065092A2/en
Publication of WO2002065092A9 publication Critical patent/WO2002065092A9/en
Publication of WO2002065092A3 publication Critical patent/WO2002065092A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A device (10) for measuring electrophysiological properties of a cell membrane of an individual cell comprises a plate (22) provided with at least one opening (58). The opening is bounded by a surface and the surface is modified, such as via heat treatment, to facilitate formation of a gigaseal. A chamber (46) is adjacent to the plate. The chamber is in fluid communication with at least one opening and is adapted to hold an electrically conductive solution. The plate further comprises a first electrode (36) located in the chamber and a second electrode (28) located adjacent to the plate.
PCT/US2002/003700 2001-02-09 2002-02-08 Devices and methods for high throughput patch clamp assays Ceased WO2002065092A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2002564562A JP2004528539A (en) 2001-02-09 2002-02-08 Apparatus and methods for high throughput patch clamp assays
EP02707739A EP1358474A2 (en) 2001-02-09 2002-02-08 Devices and methods for high throughput patch clamp assays
AU2002242128A AU2002242128A1 (en) 2001-02-09 2002-02-08 Devices and methods for high throughput patch clamp assays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/779,955 US20020108869A1 (en) 2001-02-09 2001-02-09 Device and technique for multiple channel patch clamp recordings
US09/779,955 2001-02-09
US10/029,425 US20020164777A1 (en) 2001-02-09 2001-12-21 Devices and methods for high throughput patch clamp assays
US10/029,425 2001-12-21

Publications (3)

Publication Number Publication Date
WO2002065092A2 WO2002065092A2 (en) 2002-08-22
WO2002065092A9 WO2002065092A9 (en) 2002-11-28
WO2002065092A3 true WO2002065092A3 (en) 2003-02-20

Family

ID=26704933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003700 Ceased WO2002065092A2 (en) 2001-02-09 2002-02-08 Devices and methods for high throughput patch clamp assays

Country Status (3)

Country Link
EP (1) EP1358474A2 (en)
JP (1) JP2004528539A (en)
WO (1) WO2002065092A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
EP1372828A4 (en) 2001-03-24 2008-10-29 Aviva Biosciences Corp Biochips including ion transport detecting structures and methods of use
AU2003231300A1 (en) 2002-05-04 2003-11-17 Aviva Biosciences Corporation Apparatus including ion transport detecting structures and methods of use
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
JP4552423B2 (en) 2003-11-21 2010-09-29 パナソニック株式会社 Extracellular potential measuring device and method for measuring extracellular potential using the same
JP4691407B2 (en) * 2005-06-29 2011-06-01 パナソニック株式会社 Cell potential measurement container
JP4834335B2 (en) * 2005-06-29 2011-12-14 パナソニック株式会社 Cell potential measurement container
EP1801586B1 (en) * 2002-08-21 2010-10-13 Cellectricon Ab System for obtaining and maintaining high-resistance seals in patch clamp recordings
JP4386835B2 (en) * 2002-08-21 2009-12-16 セレクトリコン アーベー System and method for acquiring and maintaining high resistance sticking in patch clamp recording
JP4661539B2 (en) * 2005-11-11 2011-03-30 パナソニック株式会社 Cell electrophysiological sensor and manufacturing method thereof
US8329009B2 (en) * 2010-04-09 2012-12-11 Molecular Devices, Llc High throughput screening of ion channels

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4324255A (en) * 1980-03-07 1982-04-13 Barach John P Method and apparatus for measuring magnetic fields and electrical currents in biological and other systems
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
US6071702A (en) * 1998-09-04 2000-06-06 Hitachi Software Engineering Co., Ltd. Probe-bearing element and method for producing the same
US20020064841A1 (en) * 2000-02-11 2002-05-30 Klemic Kathryn G. Planar patch clamp electrodes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4324255A (en) * 1980-03-07 1982-04-13 Barach John P Method and apparatus for measuring magnetic fields and electrical currents in biological and other systems
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
US6071702A (en) * 1998-09-04 2000-06-06 Hitachi Software Engineering Co., Ltd. Probe-bearing element and method for producing the same
US20020064841A1 (en) * 2000-02-11 2002-05-30 Klemic Kathryn G. Planar patch clamp electrodes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ALMERS ET AL.: "Slow calcium and potassium currents across frog muscle membrane: measurements with a vaseline-gap technique", J. PHYSIOL., vol. 312, 1981, pages 159 - 176, XP002959619 *
COSTA ET AL.: "Improved technique for studying ion channels expressed in xenopus oocytes, including fast superfusion", BIOPHYSICAL JOURNAL, vol. 67, July 1994 (1994-07-01), pages 395 - 401, XP002959618 *

Also Published As

Publication number Publication date
WO2002065092A9 (en) 2002-11-28
EP1358474A2 (en) 2003-11-05
WO2002065092A2 (en) 2002-08-22
JP2004528539A (en) 2004-09-16

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