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WO2002046463A3 - Nucleic acid extraction method and kit - Google Patents

Nucleic acid extraction method and kit Download PDF

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Publication number
WO2002046463A3
WO2002046463A3 PCT/GB2001/005370 GB0105370W WO0246463A3 WO 2002046463 A3 WO2002046463 A3 WO 2002046463A3 GB 0105370 W GB0105370 W GB 0105370W WO 0246463 A3 WO0246463 A3 WO 0246463A3
Authority
WO
WIPO (PCT)
Prior art keywords
container
temperature
nucleic acids
kit
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/005370
Other languages
French (fr)
Other versions
WO2002046463A2 (en
WO2002046463A9 (en
Inventor
Christopher Albert Dale Smith
Nathan Coombs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GENOVAR DIAGNOSTICS Ltd
Original Assignee
GENOVAR DIAGNOSTICS Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GENOVAR DIAGNOSTICS Ltd filed Critical GENOVAR DIAGNOSTICS Ltd
Priority to EP01999670A priority Critical patent/EP1417332A2/en
Priority to AU2002218417A priority patent/AU2002218417A1/en
Publication of WO2002046463A2 publication Critical patent/WO2002046463A2/en
Publication of WO2002046463A9 publication Critical patent/WO2002046463A9/en
Anticipated expiration legal-status Critical
Publication of WO2002046463A3 publication Critical patent/WO2002046463A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method and a kit for isolating nucleic acids from tissue samples. In particular, but not exclusively, formalin fixed paraffin embedded tissue samples. The isolated nucleic acids will normally be for use in a polymerase chain reaction (PCR). The inventive method comprises the sequential steps of: a) placing in a container the tissue sample, and a wax, a detergent and a tissue digestion enzyme; b) heating the container to a first temperature; c) raising the temperature of the container to a second temperature; d) cooling the container; e) forming a waxy-layer containing impurities and a nucleic acid containing layer; f) removing the waxy-layer, such that the nucleic acids are usable in a polymerase chain reaction. Kits are also provided for performing the same.
PCT/GB2001/005370 2000-12-05 2001-12-04 Nucleic acid extraction method and kit Ceased WO2002046463A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01999670A EP1417332A2 (en) 2000-12-05 2001-12-04 Nucleic acid extraction method and kit
AU2002218417A AU2002218417A1 (en) 2000-12-05 2001-12-04 Nucleic acid extraction method and kit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0029631.9 2000-12-05
GB0029631A GB2369822A (en) 2000-12-05 2000-12-05 Nucleic acid extraction method and kit

Publications (3)

Publication Number Publication Date
WO2002046463A2 WO2002046463A2 (en) 2002-06-13
WO2002046463A9 WO2002046463A9 (en) 2003-05-01
WO2002046463A3 true WO2002046463A3 (en) 2004-02-19

Family

ID=9904473

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/005370 Ceased WO2002046463A2 (en) 2000-12-05 2001-12-04 Nucleic acid extraction method and kit

Country Status (4)

Country Link
EP (1) EP1417332A2 (en)
AU (1) AU2002218417A1 (en)
GB (1) GB2369822A (en)
WO (1) WO2002046463A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1601450T3 (en) 2003-03-10 2014-03-31 Expression Pathology Inc Liquid tissue preparation from histopatologically processed biological samples, tissues and cells
WO2005054466A2 (en) † 2003-07-25 2005-06-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
EP3342877B1 (en) 2004-09-30 2020-01-29 Epigenomics AG Method for providing dna fragments derived from an archived sample
DE102005023011A1 (en) * 2005-05-19 2006-11-23 Qiagen Gmbh Method of extraction and quantified determination of intact proteins from formalin-fixed tissues, employs heat treatment for their release in absence of proteases
US9243241B2 (en) 2005-05-31 2016-01-26 Life Technologies Corporation Separation and purification of nucleic acid from paraffin-containing samples
JP2008043332A (en) * 2006-08-17 2008-02-28 Panomics Inc Quantitative determination of nucleic acid from tissue slide
EP2395082A1 (en) 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
EP3239296A1 (en) * 2010-11-30 2017-11-01 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
JP6278568B2 (en) 2011-12-06 2018-02-14 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング Recovery of aqueous phase containing biomolecules from multiphase mixtures
CN102488710A (en) * 2011-12-14 2012-06-13 吉林大学 Method for extracting thrombin antagonist from pig plasma
CN102680295B (en) * 2012-05-21 2016-01-20 江苏省中医院 A kind of method extracting residual micronucleus acid from HE staining section
JP6201587B2 (en) * 2013-09-30 2017-09-27 凸版印刷株式会社 Nucleic acid extraction method from embedded tissue, embedding agent removal tool, and nucleic acid extraction kit
CA2962782A1 (en) * 2014-09-26 2016-03-31 The Regents Of The University Of California Methods and systems for detection of a genetic mutation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0953635A1 (en) * 1998-04-28 1999-11-03 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2153215A1 (en) * 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
SE9603599D0 (en) * 1996-10-02 1996-10-02 Pharmacia Biotech Ab A method of separating a two phase system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0953635A1 (en) * 1998-04-28 1999-11-03 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AKALU A ET AL: "A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue.", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING. NETHERLANDS 15 DEC 1999, vol. 15, no. 6, 15 December 1999 (1999-12-15), pages 229 - 233, XP002242204 *
BANERJEE S K ET AL: "Microwave-Based DNA Extraction from Paraffin-Embedded Tissue for PCR Amplification.", BIOTECHNIQUES, vol. 18, no. 5, 1995, pages 768, 770, 772 - 773, XP009011206, ISSN: 0736-6205 *
CANTLAY A M ET AL: "Heterogeneous expression and polymorphic genotype of glutathione S-transferases in human lung.", THORAX, vol. 49, no. 10, 1994, pages 1010 - 1014, XP009010314, ISSN: 0040-6376 *
COOMBS N J ET AL: "Optimisation of DNA and RNA extraction from archival formalin-fixed tissue.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 AUG 1999, vol. 27, no. 16, 15 August 1999 (1999-08-15), pages e12, XP002242203, ISSN: 1362-4962 *
SLEBOS R J ET AL: "A rapid and simple procedure for the routine detection of ras point mutations in formalin-fixed, paraffin-embedded tissues.", DIAGNOSTIC MOLECULAR PATHOLOGY: THE AMERICAN JOURNAL OF SURGICAL PATHOLOGY, PART B. UNITED STATES JUN 1992, vol. 1, no. 2, June 1992 (1992-06-01), pages 136 - 141, XP009011205, ISSN: 1052-9551 *

Also Published As

Publication number Publication date
GB2369822A (en) 2002-06-12
GB0029631D0 (en) 2001-01-17
EP1417332A2 (en) 2004-05-12
WO2002046463A2 (en) 2002-06-13
AU2002218417A1 (en) 2002-06-18
WO2002046463A9 (en) 2003-05-01

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