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WO2002059353A3 - Two-step amplification using a program primer followed by specific primers - Google Patents

Two-step amplification using a program primer followed by specific primers Download PDF

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Publication number
WO2002059353A3
WO2002059353A3 PCT/CA2002/000057 CA0200057W WO02059353A3 WO 2002059353 A3 WO2002059353 A3 WO 2002059353A3 CA 0200057 W CA0200057 W CA 0200057W WO 02059353 A3 WO02059353 A3 WO 02059353A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
dna
reaction
approach
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2002/000057
Other languages
French (fr)
Other versions
WO2002059353A2 (en
Inventor
Qinyin Cai
Peng Ling
Jin-Hao Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio S & T
Original Assignee
Bio S & T
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002332610A external-priority patent/CA2332610A1/en
Application filed by Bio S & T filed Critical Bio S & T
Priority to AU2002231489A priority Critical patent/AU2002231489A1/en
Publication of WO2002059353A2 publication Critical patent/WO2002059353A2/en
Anticipated expiration legal-status Critical
Publication of WO2002059353A3 publication Critical patent/WO2002059353A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A DNA amplification approach, enabling the amplification of DNA targets with unknown sequences, involves successive PCR steps. In a first reaction, linear amplification of the target nucleic acid is achieved using one or more specially designed primers. In the second reaction, additional primers are added and exponential amplification of a double stranded DNA is achieved. Combined with conventional DNA sequencing procedures, this approach can be used for the direct sequencing of genomic DNA, avoiding the need to sub-clone large fragments. With the inventive method, moreover, sequencing information can be gathered in a much less costly and labor-intensive manner than was possible previously.
PCT/CA2002/000057 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers Ceased WO2002059353A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002231489A AU2002231489A1 (en) 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,332,610 2001-01-26
CA002332610A CA2332610A1 (en) 2001-01-26 2001-01-26 Asymmetrical pcr applification
US09/825,873 2001-04-05
US09/825,873 US20020155448A1 (en) 2001-01-26 2001-04-05 Asymmetrical PCR amplification

Publications (2)

Publication Number Publication Date
WO2002059353A2 WO2002059353A2 (en) 2002-08-01
WO2002059353A3 true WO2002059353A3 (en) 2003-08-21

Family

ID=25682361

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/000057 Ceased WO2002059353A2 (en) 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers

Country Status (1)

Country Link
WO (1) WO2002059353A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10253337B4 (en) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Method for detecting a nucleic acid
WO2006094360A1 (en) * 2005-03-11 2006-09-14 Molecular Plant Breeding Nominees Ltd Method of amplifying nucleic acid
EP1947196A4 (en) * 2005-11-08 2009-09-09 Olympus Corp Method of amplifying multiple nucleic acid sequences for differentiation
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2008154467A (en) * 2006-12-21 2008-07-10 Olympus Corp Method for amplifying nucleic acid and method for analyzing nucleic acid using the same
CN102732609B (en) * 2011-04-08 2014-03-26 博奥生物有限公司 Method for detecting similarity of oligonucleotide and target genome
HU229967B1 (en) * 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Method for determining the sequence of fragmented nucleic acids
AU2013308647B2 (en) 2012-08-30 2018-10-18 Gen-Probe Incorporated Multiphase nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
WO2016058173A1 (en) * 2014-10-17 2016-04-21 深圳华大基因研究院 Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOOTH CLAIRE ET AL: "Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 21, 1 November 2001 (2001-11-01), pages 4414 - 4422, XP002242797, ISSN: 0305-1048 *
FRITZ J D ET AL: "A NOVEL 3' EXTENSION TECHNIQUE USING RANDOM PRIMERS IN RNA-PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 13, 11 July 1991 (1991-07-11), pages 3747, XP000371658, ISSN: 0305-1048 *
PODDAR SK: "Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 14, February 2000 (2000-02-01), pages 25 - 32, XP000953250, ISSN: 0890-8508 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 13, 1992, pages 718 - 725, XP000199116, ISSN: 0888-7543 *
WELSH J ET AL: "FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 24, 25 December 1990 (1990-12-25), pages 7213 - 7218, XP000310554, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2002059353A2 (en) 2002-08-01

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