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WO2001016375A3 - Sequençage de molecules d'acide nucleique a grande vitesseen parallele - Google Patents

Sequençage de molecules d'acide nucleique a grande vitesseen parallele Download PDF

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Publication number
WO2001016375A3
WO2001016375A3 PCT/US2000/023736 US0023736W WO0116375A3 WO 2001016375 A3 WO2001016375 A3 WO 2001016375A3 US 0023736 W US0023736 W US 0023736W WO 0116375 A3 WO0116375 A3 WO 0116375A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
polymerase
nucleotide
fluorophore
acceptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/023736
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English (en)
Other versions
WO2001016375A2 (fr
Inventor
Thomas D Schneider
Denise Rubens
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US Department of Health and Human Services
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US Department of Health and Human Services
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Filing date
Publication date
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Priority to US10/070,053 priority Critical patent/US6982146B1/en
Priority to AU70868/00A priority patent/AU7086800A/en
Publication of WO2001016375A2 publication Critical patent/WO2001016375A2/fr
Publication of WO2001016375A3 publication Critical patent/WO2001016375A3/fr
Anticipated expiration legal-status Critical
Priority to US11/204,367 priority patent/US20060292583A1/en
Priority to US12/196,139 priority patent/US20090061447A1/en
Priority to US12/886,686 priority patent/US8535881B2/en
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé et un dispositif de séquençage automatisé à grande vitesse de molécules d'acide nucléique. Une molécule d'acide nucléique à séquencer est exposée à une polymérase en présence de nucléotides devant être incorporés dans un brin d'acide nucléique complémentaire. La polymérase porte un fluorophore donneur, et chaque type de nucléotide (p. ex. A, T/U, C et G) porte une caractéristique reconnaissable de fluorophore accepteur correspondant au type particulier de nucléotide. Lorsque la polymérase incorpore des molécules individuelles d'acide nucléique dans un brin complémentaire, un laser irradie en continu le fluorophore donneur, à une longueur d'onde provoquant l'émission, par celui-ci, d'un signal (la longueur d'onde du laser ne stimule toutefois pas le fluorophore accepteur). Dans des modes de réalisation particuliers, un laser n'est pas nécessaire si le fluorophore donneur est une molécule luminescente ou s'il est stimulé par une telle molécule. Le signal d'émission provenant de la polymérase est capable de stimuler n'importe lequel des fluorophores donneurs (mais pas les fluorophores accepteurs), de sorte que lorsqu'un nucléotide est ajouté par la polymérase, le fluorophore accepteur émet un signal associé au type de nucléotide ajouté au brin complémentaire. La série de signaux d'émission provenant des fluorophores accepteurs est détectée, et corrélée à une séquence de nucléotides qui correspond à la séquence des signaux d'émission.
PCT/US2000/023736 1999-08-30 2000-08-29 Sequençage de molecules d'acide nucleique a grande vitesseen parallele Ceased WO2001016375A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/070,053 US6982146B1 (en) 1999-08-30 2000-08-29 High speed parallel molecular nucleic acid sequencing
AU70868/00A AU7086800A (en) 1999-08-30 2000-08-29 High speed parallel molecular nucleic acid sequencing
US11/204,367 US20060292583A1 (en) 1999-08-30 2005-08-12 High speed parallel molecular nucleic acid sequencing
US12/196,139 US20090061447A1 (en) 1999-08-30 2008-08-21 High speed parallel molecular nucleic acid sequencing
US12/886,686 US8535881B2 (en) 1999-08-30 2010-09-21 High speed parallel molecular nucleic acid sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15158099P 1999-08-30 1999-08-30
US60/151,580 1999-08-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10070053 A-371-Of-International 2000-08-29
US11/204,367 Division US20060292583A1 (en) 1999-08-30 2005-08-12 High speed parallel molecular nucleic acid sequencing

Publications (2)

Publication Number Publication Date
WO2001016375A2 WO2001016375A2 (fr) 2001-03-08
WO2001016375A3 true WO2001016375A3 (fr) 2001-10-04

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PCT/US2000/023736 Ceased WO2001016375A2 (fr) 1999-08-30 2000-08-29 Sequençage de molecules d'acide nucleique a grande vitesseen parallele

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AU (1) AU7086800A (fr)
WO (1) WO2001016375A2 (fr)

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US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US7462449B2 (en) 1999-06-28 2008-12-09 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
US8658434B2 (en) 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
US9097667B2 (en) 2007-12-14 2015-08-04 Biotium, Inc. Fluorescent compounds
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US9133511B2 (en) 2000-10-06 2015-09-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
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US11054576B2 (en) 2015-06-12 2021-07-06 Pacific Biosciences Of California, Inc. Integrated target waveguide devices and systems for optical coupling

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US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7462449B2 (en) 1999-06-28 2008-12-09 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
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US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US9410200B2 (en) 2001-12-04 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
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US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9410199B2 (en) 2002-08-23 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9388464B2 (en) 2002-08-23 2016-07-12 Illumina Cambridge Limited Modified nucleotides
US9121060B2 (en) 2002-08-23 2015-09-01 Illumina Cambridge Limited Modified nucleotides
US9127314B2 (en) 2002-08-23 2015-09-08 Illumina Cambridge Limited Labelled nucleotides
US7491498B2 (en) 2003-11-12 2009-02-17 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
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US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9528151B2 (en) 2006-12-01 2016-12-27 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US9175342B2 (en) 2007-10-19 2015-11-03 The Trustees Of Columbia University In The City Of New York Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis
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