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WO2001094939A1 - Auto-patch monocellulaire - Google Patents

Auto-patch monocellulaire Download PDF

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Publication number
WO2001094939A1
WO2001094939A1 PCT/GB2001/002490 GB0102490W WO0194939A1 WO 2001094939 A1 WO2001094939 A1 WO 2001094939A1 GB 0102490 W GB0102490 W GB 0102490W WO 0194939 A1 WO0194939 A1 WO 0194939A1
Authority
WO
WIPO (PCT)
Prior art keywords
microchannel
cell
patch
access port
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/002490
Other languages
English (en)
Inventor
David Owen
Andrew Silverthorne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xention Discovery Ltd
Paion UK Ltd
Original Assignee
Cenes Ltd
Xention Discovery Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cenes Ltd, Xention Discovery Ltd filed Critical Cenes Ltd
Priority to EP01936652A priority Critical patent/EP1290441A1/fr
Priority to CA002411845A priority patent/CA2411845A1/fr
Priority to AU6252101A priority patent/AU6252101A/xx
Priority to AU2001262521A priority patent/AU2001262521B2/en
Publication of WO2001094939A1 publication Critical patent/WO2001094939A1/fr
Priority to US10/313,060 priority patent/US20030129581A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Definitions

  • the system is based on delivery of cells via a system of microchannels to a patch clamp pipette.
  • Cells may be pre-sorted using a fluorescence activated cell-sorter (FACS) or other methods of sorting such as immunomagnetic selection.
  • FACS fluorescence activated cell-sorter
  • the system could also be used without pre-sorting for homogenous cell populations. Delivery of cells and events leading to and including a patch clamp recording are computer controlled as is subsequent drug delivery.
  • a patch-pipette accesses cells as they pass an access port in the microchannel.
  • High- resistance electrical seals between pipette and cell are achieved by applying suction to the pipette via a suction controller either on a continuous basis or triggered by the FACS detector which also diverts cells with an appropriate fluorescence signal or light scattering properties along the appropriate microchannel.
  • the minimal system would consist of a single microchannel with patch-clamp module. Cell suspensions are pumped (eg. using a peristaltic pump) from a cell incubator through the microchannel.
  • More sophisticated and also higher throughput devices would have a FACS with multi-wavelength capability to permit selection of several cell-types and also multiple patch-clamp modules to permit parallel recording from many cells which may be different or the same in respect to their fluorescence or cell-scattering 'signature'.
  • a perfusion flow controller switches the flow of solution through the microchannel from delivering cells to delivering drug solutions and the experiment is initiated.
  • Patch-clamp modules may be cascaded such that in the event more than one cell is detected by the FACS, multiple recordings may be made. Excess cells are simply recycled back to the cell incubator.
  • the FACS front end is not required although it would have the beneficial effect of eliminating debris from the system. In the case where cells come from a mixed background a FACS front end allows selection of even minor components of the overall cell suspension.
  • the system can be fully automated and because it also recycles cells and solutions, can run for extended periods of time without intervention.
  • a device for supplying conventional glass patch pipettes will be incorporated or alternatively a system for rejuvenating and hence re-using quartz glass pipettes will be used.
  • Data obtained can be automatically downloaded to a server for off-line analysis etc. without interrupting data acquisition.
  • FIG. 1 Flow Patch-Clamp System fluorescence-activated cell sorter (FACS)- optional cell incubator patch-clamp module control and data acquisition interface perfusion flow controller sorted cells: channel #1 sorted cells: channel #2 unsorted cells channel return channel to cell incubator
  • FACS Fluorescence-activated cell sorter

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un procédé permettant de réaliser à partir d'une seule cellule un enregistrement selon la technique patch-clamp. On commence par établir un micro-canal admettant un écoulement axial du liquide. Dans ce micro-canal, on ménage au moins un orifice permettant un accès radial depuis l'extérieur du micro-canal (air) vers l'intérieur du micro-canal (liquide), le liquide formant à l'intérieur du micro-canal au niveau de l'orifice un ménisque constituant une interface air-liquide. Avec une pipette pour procédé patch-clamp dont la pointe, capable de passer dans l'orifice d'accès, permet de former entre la pointe et la cellule une fermeture électrique à haute résistance, c'est-à-dire giga-ohmique, on laisse passer axialement dans la longueur du micro-canal le liquide portant la cellule, ce qui amène la cellule jusqu'à l'orifice d'accès. Après avoir déplacé la pointe de pipette radialement par rapport au micro-canal jusqu'à toucher l'interface air-liquide dans l'orifice d'accès, on applique au patch-clamp une force d'aspiration de façon à attirer sur la pointe la cellule pour former une fermeture. Il ne reste plus qu'à réaliser l'enregistrement en patch-clamp. L'invention concerne également un appareil pour mettre en oeuvre ce procédé.
PCT/GB2001/002490 2000-06-06 2001-06-06 Auto-patch monocellulaire Ceased WO2001094939A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP01936652A EP1290441A1 (fr) 2000-06-06 2001-06-06 Auto-patch monocellulaire
CA002411845A CA2411845A1 (fr) 2000-06-06 2001-06-06 Auto-patch monocellulaire
AU6252101A AU6252101A (en) 2000-06-06 2001-06-06 Single cell auto patch
AU2001262521A AU2001262521B2 (en) 2000-06-06 2001-06-06 Single cell auto patch
US10/313,060 US20030129581A1 (en) 2000-06-06 2002-12-06 Patch-clamping method and apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0013584A GB0013584D0 (en) 2000-06-06 2000-06-06 Automated flow patch-clamp system
GB0013584.8 2000-06-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/313,060 Continuation-In-Part US20030129581A1 (en) 2000-06-06 2002-12-06 Patch-clamping method and apparatus

Publications (1)

Publication Number Publication Date
WO2001094939A1 true WO2001094939A1 (fr) 2001-12-13

Family

ID=9892963

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002490 Ceased WO2001094939A1 (fr) 2000-06-06 2001-06-06 Auto-patch monocellulaire

Country Status (5)

Country Link
EP (1) EP1290441A1 (fr)
AU (2) AU2001262521B2 (fr)
CA (1) CA2411845A1 (fr)
GB (1) GB0013584D0 (fr)
WO (1) WO2001094939A1 (fr)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004034052A1 (fr) * 2002-10-09 2004-04-22 St. Boniface General Hospital Systeme de dosage a haut rendement
ES2208082A1 (es) * 2002-05-29 2004-06-01 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
US6936462B1 (en) 1998-06-12 2005-08-30 Xention Discovery Limited High throughput screen
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
US7201836B2 (en) 1997-12-17 2007-04-10 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US7384733B1 (en) 1998-12-05 2008-06-10 Xention Discovery Limited Interface patch clamping
US7563614B2 (en) * 2002-02-12 2009-07-21 Cellectricon Ab Systems and methods for rapidly changing the solution environment around sensors
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
WO2012155973A1 (fr) 2011-05-19 2012-11-22 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Procédé et dispositif pour la détermination automatique de la position d'un microsystème pour la manipulation d'un micro-objet sphérique
US8338150B2 (en) 1997-11-06 2012-12-25 Cellectricon Ab Method for combined parallel agent delivery and electroporation for cell structures an use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998055870A1 (fr) * 1997-06-04 1998-12-10 A+ Science Invest Ab Detection de molecules biologiquement actives a l'aide de capteurs biologiques pre-actives a base de cellule dans des systemes de separation a base de liquide
US6063260A (en) * 1994-10-28 2000-05-16 Neurosearch A/S Patch clamp apparatus and technique having high throughput and low fluid volume requirements
WO2000034776A1 (fr) * 1998-12-05 2000-06-15 Cenes Limited Interface patch clamp

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063260A (en) * 1994-10-28 2000-05-16 Neurosearch A/S Patch clamp apparatus and technique having high throughput and low fluid volume requirements
WO1998055870A1 (fr) * 1997-06-04 1998-12-10 A+ Science Invest Ab Detection de molecules biologiquement actives a l'aide de capteurs biologiques pre-actives a base de cellule dans des systemes de separation a base de liquide
WO2000034776A1 (fr) * 1998-12-05 2000-06-15 Cenes Limited Interface patch clamp

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAMILL O P ET AL: "IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES", PFLUEGERS ARCHIV, SPRINGER VERLAG, BERLIN, DE, vol. 391, 1981, pages 85 - 100, XP000196663, ISSN: 0031-6768 *

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8338150B2 (en) 1997-11-06 2012-12-25 Cellectricon Ab Method for combined parallel agent delivery and electroporation for cell structures an use thereof
US7201836B2 (en) 1997-12-17 2007-04-10 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US7387715B2 (en) 1997-12-17 2008-06-17 Molecular Devices Corporation Sample positioning and analysis system
US10006902B2 (en) 1998-06-12 2018-06-26 Sophion Bioscience A/S High throughput screen
US6936462B1 (en) 1998-06-12 2005-08-30 Xention Discovery Limited High throughput screen
US7384733B1 (en) 1998-12-05 2008-06-10 Xention Discovery Limited Interface patch clamping
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
US7563614B2 (en) * 2002-02-12 2009-07-21 Cellectricon Ab Systems and methods for rapidly changing the solution environment around sensors
ES2208082A1 (es) * 2002-05-29 2004-06-01 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
WO2004034052A1 (fr) * 2002-10-09 2004-04-22 St. Boniface General Hospital Systeme de dosage a haut rendement
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
WO2012155973A1 (fr) 2011-05-19 2012-11-22 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Procédé et dispositif pour la détermination automatique de la position d'un microsystème pour la manipulation d'un micro-objet sphérique
US11054409B2 (en) 2011-05-19 2021-07-06 Multichannel Systems Mcs Gmbh Method and device for automatically determining the position of a microsystem for manipulating a spherical microobject

Also Published As

Publication number Publication date
EP1290441A1 (fr) 2003-03-12
CA2411845A1 (fr) 2001-12-13
GB0013584D0 (en) 2000-07-26
AU2001262521B2 (en) 2005-07-14
AU6252101A (en) 2001-12-17

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