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EP1290441A1 - Auto-patch monocellulaire - Google Patents

Auto-patch monocellulaire

Info

Publication number
EP1290441A1
EP1290441A1 EP01936652A EP01936652A EP1290441A1 EP 1290441 A1 EP1290441 A1 EP 1290441A1 EP 01936652 A EP01936652 A EP 01936652A EP 01936652 A EP01936652 A EP 01936652A EP 1290441 A1 EP1290441 A1 EP 1290441A1
Authority
EP
European Patent Office
Prior art keywords
microchannel
cell
patch
access port
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01936652A
Other languages
German (de)
English (en)
Inventor
David CeNes Limited OWEN
Andrew CeNeS Limited SILVERTHORNE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xention Discovery Ltd
Original Assignee
Xention Discovery Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xention Discovery Ltd filed Critical Xention Discovery Ltd
Publication of EP1290441A1 publication Critical patent/EP1290441A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Definitions

  • the system is based on delivery of cells via a system of microchannels to a patch clamp pipette.
  • Cells may be pre-sorted using a fluorescence activated cell-sorter (FACS) or other methods of sorting such as immunomagnetic selection.
  • FACS fluorescence activated cell-sorter
  • the system could also be used without pre-sorting for homogenous cell populations. Delivery of cells and events leading to and including a patch clamp recording are computer controlled as is subsequent drug delivery.
  • a patch-pipette accesses cells as they pass an access port in the microchannel.
  • High- resistance electrical seals between pipette and cell are achieved by applying suction to the pipette via a suction controller either on a continuous basis or triggered by the FACS detector which also diverts cells with an appropriate fluorescence signal or light scattering properties along the appropriate microchannel.
  • the minimal system would consist of a single microchannel with patch-clamp module. Cell suspensions are pumped (eg. using a peristaltic pump) from a cell incubator through the microchannel.
  • More sophisticated and also higher throughput devices would have a FACS with multi-wavelength capability to permit selection of several cell-types and also multiple patch-clamp modules to permit parallel recording from many cells which may be different or the same in respect to their fluorescence or cell-scattering 'signature'.
  • a perfusion flow controller switches the flow of solution through the microchannel from delivering cells to delivering drug solutions and the experiment is initiated.
  • Patch-clamp modules may be cascaded such that in the event more than one cell is detected by the FACS, multiple recordings may be made. Excess cells are simply recycled back to the cell incubator.
  • the FACS front end is not required although it would have the beneficial effect of eliminating debris from the system. In the case where cells come from a mixed background a FACS front end allows selection of even minor components of the overall cell suspension.
  • the system can be fully automated and because it also recycles cells and solutions, can run for extended periods of time without intervention.
  • a device for supplying conventional glass patch pipettes will be incorporated or alternatively a system for rejuvenating and hence re-using quartz glass pipettes will be used.
  • Data obtained can be automatically downloaded to a server for off-line analysis etc. without interrupting data acquisition.
  • FIG. 1 Flow Patch-Clamp System fluorescence-activated cell sorter (FACS)- optional cell incubator patch-clamp module control and data acquisition interface perfusion flow controller sorted cells: channel #1 sorted cells: channel #2 unsorted cells channel return channel to cell incubator
  • FACS Fluorescence-activated cell sorter

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un procédé permettant de réaliser à partir d'une seule cellule un enregistrement selon la technique patch-clamp. On commence par établir un micro-canal admettant un écoulement axial du liquide. Dans ce micro-canal, on ménage au moins un orifice permettant un accès radial depuis l'extérieur du micro-canal (air) vers l'intérieur du micro-canal (liquide), le liquide formant à l'intérieur du micro-canal au niveau de l'orifice un ménisque constituant une interface air-liquide. Avec une pipette pour procédé patch-clamp dont la pointe, capable de passer dans l'orifice d'accès, permet de former entre la pointe et la cellule une fermeture électrique à haute résistance, c'est-à-dire giga-ohmique, on laisse passer axialement dans la longueur du micro-canal le liquide portant la cellule, ce qui amène la cellule jusqu'à l'orifice d'accès. Après avoir déplacé la pointe de pipette radialement par rapport au micro-canal jusqu'à toucher l'interface air-liquide dans l'orifice d'accès, on applique au patch-clamp une force d'aspiration de façon à attirer sur la pointe la cellule pour former une fermeture. Il ne reste plus qu'à réaliser l'enregistrement en patch-clamp. L'invention concerne également un appareil pour mettre en oeuvre ce procédé.
EP01936652A 2000-06-06 2001-06-06 Auto-patch monocellulaire Withdrawn EP1290441A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0013584A GB0013584D0 (en) 2000-06-06 2000-06-06 Automated flow patch-clamp system
GB0013584 2000-06-06
PCT/GB2001/002490 WO2001094939A1 (fr) 2000-06-06 2001-06-06 Auto-patch monocellulaire

Publications (1)

Publication Number Publication Date
EP1290441A1 true EP1290441A1 (fr) 2003-03-12

Family

ID=9892963

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01936652A Withdrawn EP1290441A1 (fr) 2000-06-06 2001-06-06 Auto-patch monocellulaire

Country Status (5)

Country Link
EP (1) EP1290441A1 (fr)
AU (2) AU6252101A (fr)
CA (1) CA2411845A1 (fr)
GB (1) GB0013584D0 (fr)
WO (1) WO2001094939A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003046170A1 (fr) 2001-11-27 2003-06-05 Cellectricon Ab Procede d'administration parallele combinee d'agents et d'electroporation pour structures cellulaires et utilisation associee
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
KR100736059B1 (ko) 1998-12-05 2007-07-06 젠션 디스커버리 리미티드 인터페이스 패치 클램핑
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
EP2368636A3 (fr) * 2002-02-12 2012-03-07 Cellectricon Ab Systèmes et procédés de changement rapide de l'environnement de solution autour de capteurs
ES2208082B1 (es) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
CA2502238A1 (fr) * 2002-10-09 2004-04-22 Larry Hryshko Systeme de dosage a haut rendement
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
DK2710109T3 (en) 2011-05-19 2018-01-22 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Univ Tuebingen Method and use of a device for automated positioning of a microsystem for manipulating a spherical micro object

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE216080T1 (de) * 1994-10-28 2002-04-15 Sophion Bioscience As Patch-clamp-vorrichtung und -technik mit hohem durchfluss und niedrigen flüssigkeitsvolumenanforderungen
SE9702112D0 (sv) * 1997-06-04 1997-06-04 Holdingbolaget Vid Goeteborgs Method and apparatus for detection of a receptor antagonist
KR100736059B1 (ko) * 1998-12-05 2007-07-06 젠션 디스커버리 리미티드 인터페이스 패치 클램핑

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0194939A1 *

Also Published As

Publication number Publication date
WO2001094939A1 (fr) 2001-12-13
AU6252101A (en) 2001-12-17
GB0013584D0 (en) 2000-07-26
CA2411845A1 (fr) 2001-12-13
AU2001262521B2 (en) 2005-07-14

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