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WO2001079422A2 - Tete de prelevement amelioree - Google Patents

Tete de prelevement amelioree Download PDF

Info

Publication number
WO2001079422A2
WO2001079422A2 PCT/EP2001/004387 EP0104387W WO0179422A2 WO 2001079422 A2 WO2001079422 A2 WO 2001079422A2 EP 0104387 W EP0104387 W EP 0104387W WO 0179422 A2 WO0179422 A2 WO 0179422A2
Authority
WO
WIPO (PCT)
Prior art keywords
needle
picker head
gel
spot picker
accordance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/004387
Other languages
English (en)
Other versions
WO2001079422A3 (fr
Inventor
Anna-Karin Brundin Storm
Jozef Szabo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Pharmacia Biotech AB
Amersham Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0001463A external-priority patent/SE0001463D0/xx
Application filed by Amersham Pharmacia Biotech AB, Amersham Bioscience AB filed Critical Amersham Pharmacia Biotech AB
Priority to AU2001262205A priority Critical patent/AU2001262205A1/en
Publication of WO2001079422A2 publication Critical patent/WO2001079422A2/fr
Publication of WO2001079422A3 publication Critical patent/WO2001079422A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0657—Pipetting powder
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • the present invention relates to devices and methods for picking up spots of gel from sheets of gel.
  • biochemical analytical methods produce results in the form of sheets of gel upon which the analysed substances are grouped in spots. Further analyse of the substances can be made by cutting out and picking up the cylindrical plugs of gel containing the substances of interest and transferring the plugs to other devices such as microtitre plates or test tubes for further analysis.
  • the plugs are extracted from the sheet of gel by lowering a cylindrical hollow needle through liquid covering the gel and the gel until it reaches the supporting base plate, applying a suction to the upper end of the needle in order to lift the plug off the base plate and into the needle where it is caught by a pieced bulkhead near the tip of the needle.
  • the plug can then be transported by the picker head to a position above a well in a micro-titre plate or a test tube and then ejected out of the needle by pressurised liquid applied to the upper part of the needle.
  • a device suitable for performing this method is known from JP 3240482.
  • a problem with this method is that some of the liquid covering the gel can often collect around the base of the needle where it is mounted in the picking head.
  • the ejecting liquid will follow the plug into the well. This liquid is ejected with a high velocity and may bounce back up the sides or bottom of the well, drawing the plug with it. If this ej ected liquid contacts the liquid already surrounding the base of the needle then capillary action will tend to draw the ejected liquid into the liquid around the base. This will also draw the plug with it, leading to the plug becoming attached to the outside of the needle instead of being in the well.
  • the purpose of the present invention is to overcome the problems of the prior art spot pickings devices. This is achieved by a device having the characterising features of claim 1.
  • the present invention will be described in more detail below by means of non-limiting examples of preferred embodiments and with reference to the following drawings.
  • Figure 1 shows a lateral view of a spot picker head in accordance with the prior art
  • Figure 2 shows a section through line I-I in figure 1 ;
  • Figure 4 shows a lateral view of a spot picker head in accordance with the present invention
  • Figure 5 shows a section through line IN-IN in figure 4.
  • Figure 1 shows a lateral view of a prior art picker head 1 for picking up plugs of gel from a sheet of gel 2.
  • the sheet of gel 2 is support on a plate 3 and is covered by a thin layer of fluid 4.
  • Picker head 1 has a tubular body 5.
  • the upper end of body 5 is adapted by means of complementing fastening means (which are well known in the prior art and therefore not shown) to be attached to a picker arm (not shown).
  • the lower end of body 5 has an opening 6 to which a hollow needle 7 is attached. Needle 7 is open at each end.
  • the upper end of body 5 can be connected to a supply of liquid at a pressure lower than atmospheric pressure in order to form a suction at the lower end of needle 7 and it can also be connected to a supply of pressurised liquid in order to form an overpressure at the lower end of needle 7.
  • Needle 7 contains a bulkhead 9 with a centre hole 11. When a gel plug 13 is sucked up from the plate 3, the bulkhead 9 prevents it from being sucked all the way up into the body 5.
  • Picker head 1 is shown in figure 1 in a position where it has been lowered through the fluid 4 and the sheet of gel 2 and is in contact with the plate 3.
  • the needle contains a plug 13 of gel.
  • the gel can be lifted of the plate 3 by connecting a supply of liquid at a pressure lower than atmospheric pressure to the upper end of body 5. This sucks the gel plug 13 off the plate 3 and draws it and some of the fluid 4 up into the needle 7 until the gel plug comes into contact with the bulkhead 9.
  • Figure is a cross-section through line I-I of figure 1.
  • Figures 4 and 5 shown embodiments of a picker head in accordance with the present invention.
  • a picker head 41 having a body 45 and a needle 47.
  • the needle In order to allow the needle to be made small but strong enough to withstand the forces applied when it is pressed though the gel 2 and against the underlying plate 3, it is preferable that it is made of a high-strength material such as a titanium alloy (e.g. titanium grade 5) or a ceramic material (e.g. aluminium oxide) or the like.
  • the needle 47 is provided with a hydrophobic coating 49 (not drawn to scale) which covers parts of the exterior parts of the body 45 and needle 47 which during use are not intended to be in contact with a fluid but which accidentally may collect fluid.
  • substantially the whole of the exterior surface of the needle 47 is provided with this hydrophobic coating 49 as well as the exterior of the bottom end of the body 45.
  • the hydrophobic layer 49 can preferably be made of a thin layer of Teflon TM (PTFE) which is hydrophobic and has the advantage that it can be applied in very thin layers, thereby keeping the diameter of the needle 47 small.
  • PTFE Teflon TM
  • Another suitable coating is varnish, which, while being cheap and easy to (re)apply, suffers from the disadvantage that it forms a relatively thick coating when compared to Teflon TM.
  • the exterior wall of body 45 is also at least partially covered with a hydrophobic coating 49'.
  • the needle is made of a material that is hydrophobic, e.g. Teflon TM or treated to become hydrophobic. It is conceivable in this embodiment to also make the body of the picker head out of a hydrophobic material.
  • part of the picker head e.g. the needle or body
  • the other part of the picker head e.g. the body or needle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Manipulator (AREA)
  • Container, Conveyance, Adherence, Positioning, Of Wafer (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne une tête de prélèvement d'échantillons comprenant un corps (45) et une aiguille creuse (47) dépassant de ladite tête, dans laquelle la quasi-totalité de la surface extérieure de l'aiguille (47) est pourvue d'un revêtement hydrophobe (49), tel que du Téflon (PTFE).
PCT/EP2001/004387 2000-04-18 2001-04-18 Tete de prelevement amelioree Ceased WO2001079422A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001262205A AU2001262205A1 (en) 2000-04-18 2001-04-18 Improved spot picker head

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0001463-9 2000-04-18
SE0001463A SE0001463D0 (sv) 2000-04-18 2000-04-18 Improved spot picker head
SE0002003A SE0002003D0 (sv) 2000-04-18 2000-05-26 Improved spot picker head
SE0002003-2 2000-05-26

Publications (2)

Publication Number Publication Date
WO2001079422A2 true WO2001079422A2 (fr) 2001-10-25
WO2001079422A3 WO2001079422A3 (fr) 2002-05-16

Family

ID=26655087

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/004387 Ceased WO2001079422A2 (fr) 2000-04-18 2001-04-18 Tete de prelevement amelioree

Country Status (3)

Country Link
AU (1) AU2001262205A1 (fr)
SE (1) SE0002003D0 (fr)
WO (1) WO2001079422A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1795264A1 (fr) 2006-07-06 2007-06-13 Agilent Technologies, Inc. aiguille hydrofuge

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE758245A (fr) * 1969-11-03 1971-04-30 Hoffmann La Roche Pipette de precision
FR2071052A5 (en) * 1969-12-16 1971-09-17 Automatisme Cie Gle Liquid sampling technique - to provide exact portions - without contamination of appts
DE2164363A1 (de) * 1971-12-23 1973-07-05 Allan Costa Injektionsnadel fuer injektionsspritzen
GB1394405A (en) * 1973-01-26 1975-05-14 Shandon Southern Instr Ltd Gel cutters
US4249541A (en) * 1979-04-26 1981-02-10 David S. Pratt Biopsy device
DE3218467C2 (de) * 1982-05-15 1986-01-09 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Vorrichtung zur Mikro-Probennahme und Verfahren zur Mikro-Probenaufnahme und -abgabe mit einer solchen Vorrichtung
FR2528315A1 (fr) * 1982-06-09 1983-12-16 Klotz Antoine Sonde de prelevement et d'injection
US5080655A (en) * 1988-05-26 1992-01-14 Haaga John R Medical biopsy needle
US6296702B1 (en) * 1999-03-15 2001-10-02 Pe Corporation (Ny) Apparatus and method for spotting a substrate

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1795264A1 (fr) 2006-07-06 2007-06-13 Agilent Technologies, Inc. aiguille hydrofuge

Also Published As

Publication number Publication date
SE0002003D0 (sv) 2000-05-26
WO2001079422A3 (fr) 2002-05-16
AU2001262205A1 (en) 2001-10-30

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