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WO2001060840A3 - Estimation des proteines/de la solubilite et du repliage par complementation structurelle - Google Patents

Estimation des proteines/de la solubilite et du repliage par complementation structurelle Download PDF

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Publication number
WO2001060840A3
WO2001060840A3 PCT/US2001/005097 US0105097W WO0160840A3 WO 2001060840 A3 WO2001060840 A3 WO 2001060840A3 US 0105097 W US0105097 W US 0105097W WO 0160840 A3 WO0160840 A3 WO 0160840A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
folding
improper
marker protein
protein solubility
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/005097
Other languages
English (en)
Other versions
WO2001060840A2 (fr
Inventor
Philip Jordan Thomas
John F Hunt
William Christian Wigley
Rhesa D Stidham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
University of Texas System
University of Texas at Austin
Original Assignee
Columbia University in the City of New York
University of Texas System
University of Texas at Austin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Columbia University in the City of New York, University of Texas System, University of Texas at Austin filed Critical Columbia University in the City of New York
Priority to AU3705501A priority Critical patent/AU3705501A/xx
Priority to DE60123755T priority patent/DE60123755T2/de
Priority to CA2398834A priority patent/CA2398834C/fr
Priority to DK01909281T priority patent/DK1257829T3/da
Priority to AU2001237055A priority patent/AU2001237055B2/en
Priority to EP01909281A priority patent/EP1257829B1/fr
Priority to JP2001560224A priority patent/JP4772247B2/ja
Publication of WO2001060840A2 publication Critical patent/WO2001060840A2/fr
Publication of WO2001060840A3 publication Critical patent/WO2001060840A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Plusieurs protéines, lorsqu'elles sont produites par recombinaison, souffrent d'un traitement et d'un repliage non appropriés et manquent de solubilité normale. Les protéines modifiées, y compris celles indiquant des états pathologiques peuvent également avoir ces défauts. La présente invention concerne des procédés pour identifier le repliage approprié ou non approprié, un traitement erroné et/ou l'insolubilité. Le procédé est fondé sur l'utilisation de deux composants: une protéine de fusion spécialisée et une complémentation structurelle. La protéine de fusion contient des séquences provenant de la protéine d'intérêt et une partie de la protéine de marquage qui elle-même n'est pas active. Une cellule hôte fournit le reste de la protéine de marquage qui sert à 'complémenter' la fonction de la protéine de marquage fusionnée de manière à ce que leur association rétablisse l'activité en permettant la détection.
PCT/US2001/005097 2000-02-14 2001-02-14 Estimation des proteines/de la solubilite et du repliage par complementation structurelle Ceased WO2001060840A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU3705501A AU3705501A (en) 2000-02-14 2001-02-14 Protein/solubility folding assessed by structural complementation
DE60123755T DE60123755T2 (de) 2000-02-14 2001-02-14 Komplementierungsassay für den nachweis von protein-löslichkeit und -faltung
CA2398834A CA2398834C (fr) 2000-02-14 2001-02-14 Repliement proteique/de solubilite evalue par complementation structurale
DK01909281T DK1257829T3 (da) 2000-02-14 2001-02-14 Komplementationsassay der vurderer proteinoplöselighed og -foldning
AU2001237055A AU2001237055B2 (en) 2000-02-14 2001-02-14 Complementation assay assessing protein solubility and folding
EP01909281A EP1257829B1 (fr) 2000-02-14 2001-02-14 Estimation de la solubilite et du repliage des proteines par complementation structurelle
JP2001560224A JP4772247B2 (ja) 2000-02-14 2001-02-14 タンパク質の溶解性及びフォールディングを評価する相補性アッセイ

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18228300P 2000-02-14 2000-02-14
US60/182,283 2000-02-14
US77505100A 2001-01-31 2001-01-31
US09/775,051 2001-01-31

Publications (2)

Publication Number Publication Date
WO2001060840A2 WO2001060840A2 (fr) 2001-08-23
WO2001060840A3 true WO2001060840A3 (fr) 2002-04-25

Family

ID=22667801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/005097 Ceased WO2001060840A2 (fr) 2000-02-14 2001-02-14 Estimation des proteines/de la solubilite et du repliage par complementation structurelle

Country Status (1)

Country Link
WO (1) WO2001060840A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9511074B2 (en) 2009-08-03 2016-12-06 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of treating disorders associated with protein polymerization
US9844605B2 (en) * 2009-11-05 2017-12-19 The University of Pittsburgh—Of the Commonwealth System of Higher Education Transgenic Caenorhabditis elegans comprising a human protein with a tendency to aggregate fused to a fluorescent protein

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2883886B1 (fr) * 2005-04-05 2010-12-03 Biomethodes Methode d'obtention de variants solubles ou mieux exprimes d'une proteine d'interet
JP5303448B2 (ja) 2006-03-13 2013-10-02 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 親和性が減少した酵素の相補性レポーター系を用いた分子相互作用の検出
US8101373B2 (en) 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
US9072772B2 (en) 2009-11-05 2015-07-07 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of treating disorders associated with protein aggregation
US8569057B2 (en) 2011-06-23 2013-10-29 Discoverx Corporation Monitoring protein trafficking using beta-galactosidase reporter fragment complementation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034120A1 (fr) * 1997-01-31 1998-08-06 Universite De Montreal Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires
WO1998044350A1 (fr) * 1997-04-02 1998-10-08 The Board Of Trustees Of The Leland Stanford Junior University Detection d'interactions moleculaires par complementation de sous-unites de marquage

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ABBAS-TERKI TOUFIK ET AL: "alpha-complemented beta-galactosidase. An in vivo model substrate for the molecular chaperone heat-shock protein 90 in yeast.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 266, no. 2, December 1999 (1999-12-01), pages 517 - 523, XP002182770, ISSN: 0014-2956 *
CAMPBELL VALOIS F -X ET AL: "Utilisation of the PCA strategy to study the folding of the RBD of raf.", FASEB JOURNAL, vol. 13, no. 7, 23 April 1999 (1999-04-23), Annual Meeting of the American Societies for Experimental Biology on Biochemistry and Molecular Biology 99;San Francisco, California, USA; May 16-20, 1999, pages A1387, XP002182768, ISSN: 0892-6638 *
JOHNSSON N ET AL: "SPLIT UBIQUITIN AS A SENSOR OF PROTEIN INTERACTIONS INV VIVO", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 October 1994 (1994-10-01), pages 10340 - 10344, XP002064564, ISSN: 0027-8424 *
KO Y H ET AL: "THE CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR. OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF WILD TYPE ANDDELTAF508 MUTANT FORMS OF THE FIRST NUCLEOTIDE BINDING FOLD IN FUSION WITH THE MALTOSE-BINDING PROTEIN", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 268, no. 32, 15 November 1993 (1993-11-15), pages 24330 - 24338, XP002055212, ISSN: 0092-8674 *
NIXON ANDREW E ET AL: "Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme.", PROTEIN ENGINEERING, vol. 13, no. 5, May 2000 (2000-05-01), pages 323 - 327, XP002182771, ISSN: 0269-2139 *
WALDO GEOFFREY S ET AL: "Rapid protein-folding assay using green fluorescent protein", NATURE BIOTECHNOLOGY, vol. 17, July 1999 (1999-07-01), pages 691 - 695, XP002182769 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9511074B2 (en) 2009-08-03 2016-12-06 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of treating disorders associated with protein polymerization
US9844605B2 (en) * 2009-11-05 2017-12-19 The University of Pittsburgh—Of the Commonwealth System of Higher Education Transgenic Caenorhabditis elegans comprising a human protein with a tendency to aggregate fused to a fluorescent protein

Also Published As

Publication number Publication date
WO2001060840A2 (fr) 2001-08-23

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