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WO2001048181A3 - Vorrichtung zum transfer von molekülen in zellen - Google Patents

Vorrichtung zum transfer von molekülen in zellen Download PDF

Info

Publication number
WO2001048181A3
WO2001048181A3 PCT/DE2000/004631 DE0004631W WO0148181A3 WO 2001048181 A3 WO2001048181 A3 WO 2001048181A3 DE 0004631 W DE0004631 W DE 0004631W WO 0148181 A3 WO0148181 A3 WO 0148181A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
molecules
medium
transferring molecules
acoustic pulses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2000/004631
Other languages
English (en)
French (fr)
Other versions
WO2001048181A2 (de
Inventor
Friedrich Ueberle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dornier Medizintechnik GmbH
Original Assignee
Dornier Medizintechnik GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE1999162904 external-priority patent/DE19962904A1/de
Application filed by Dornier Medizintechnik GmbH filed Critical Dornier Medizintechnik GmbH
Priority to EP00990583A priority Critical patent/EP1244770A2/de
Priority to JP2001548694A priority patent/JP2003533974A/ja
Priority to CA002397271A priority patent/CA2397271A1/en
Publication of WO2001048181A2 publication Critical patent/WO2001048181A2/de
Publication of WO2001048181A3 publication Critical patent/WO2001048181A3/de
Priority to US10/177,823 priority patent/US20030017578A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Ein Medium, in welchem sich Zellen und in diese Zellen zu transferierende Moleküle befinden, wird innerhalb einer hohlzylindrischen Vorrichtung zum Erzeugen akustischer, Kavitation bewirkender Pulse relativ zu einem Bereich, in dem die akustischen Pulse fokusiert auftreten, bewegt.
PCT/DE2000/004631 1999-12-23 2000-12-23 Vorrichtung zum transfer von molekülen in zellen Ceased WO2001048181A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00990583A EP1244770A2 (de) 1999-12-23 2000-12-23 Vorrichtung zum transfer von molekülen in zellen
JP2001548694A JP2003533974A (ja) 1999-12-23 2000-12-23 細胞内に分子を導入する装置
CA002397271A CA2397271A1 (en) 1999-12-23 2000-12-23 Method for transferring molecules in cells
US10/177,823 US20030017578A1 (en) 1999-12-23 2002-06-21 Apparatus for transferring molecules into cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19962904.8 1999-12-23
DE1999162904 DE19962904A1 (de) 1999-12-23 1999-12-23 Verfahren zum Transfer von Molekülen in Zellen und Vorrichtung zur Durchführung des Verfahrens
DE10063942.9 2000-12-20
DE10063942 2000-12-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/177,823 Continuation US20030017578A1 (en) 1999-12-23 2002-06-21 Apparatus for transferring molecules into cells

Publications (2)

Publication Number Publication Date
WO2001048181A2 WO2001048181A2 (de) 2001-07-05
WO2001048181A3 true WO2001048181A3 (de) 2002-04-18

Family

ID=26008022

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/004631 Ceased WO2001048181A2 (de) 1999-12-23 2000-12-23 Vorrichtung zum transfer von molekülen in zellen

Country Status (5)

Country Link
US (1) US20030017578A1 (de)
EP (1) EP1244770A2 (de)
JP (1) JP2003533974A (de)
CA (1) CA2397271A1 (de)
WO (1) WO2001048181A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10108799A1 (de) * 2001-02-19 2002-09-05 Laser & Med Tech Gmbh Verfahren und Vorrichtung zur Ultraschallimpfung von biologischem Zellmaterial
DE10211886B4 (de) * 2002-03-18 2004-07-15 Dornier Medtech Gmbh Verfahren und Einrichtung zum Erzeugen bipolarer akustischer Impulse
DE10223196B4 (de) * 2002-05-24 2004-05-13 Dornier Medtech Systems Gmbh Verfahren und Einrichtung zum Transferieren von Molekülen in Zellen
IL149932A0 (en) 2002-05-30 2002-11-10 Nano Size Ltd High power ultrasonic reactor and process for ultrasonic treatment of a reaction material
AU2003290740A1 (en) * 2003-12-01 2005-08-12 Richard E. Walters Non-uniform electric field chamber for cell fusion
JP4804906B2 (ja) 2004-12-15 2011-11-02 ドルニエル メドテック システムズ ゲーエムベーハー 循環器および神経疾患の患者における細胞治療および組織再生の衝撃波による改良方法
FR2957532B1 (fr) * 2010-03-19 2012-09-28 Commissariat Energie Atomique Agitateur d'un echantillon liquide
US11046596B2 (en) 2012-10-25 2021-06-29 Hydrus Technology Pty. Ltd. Electrochemical liquid treatment apparatus
US11046595B2 (en) 2014-05-23 2021-06-29 Hydrus Technology Pty. Ltd. Electrochemical treatment methods
US10850994B2 (en) 2014-05-23 2020-12-01 Hydrus Technology Pty. Ltd. Electrochemical liquid treatment apparatus
CN107429262A (zh) * 2015-01-07 2017-12-01 英迪公司 一种用于机械和水动力微流体转染的方法以及用于其的设备
KR102232757B1 (ko) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 체외충격파를 이용한 표적물질 전달 장치

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989002464A1 (en) * 1987-09-07 1989-03-23 Amersham International Plc Modifying living cells
DE3821354A1 (de) * 1987-07-10 1989-07-06 Berlin Kosmetik Veb Verfahren und vorrichtung zur herstellung bioaktiver suspensionen
EP0326701A2 (de) * 1988-02-04 1989-08-09 Dornier Medizintechnik Gmbh Piezoelektrische Stosswellenquelle
WO1999058637A2 (de) * 1998-05-07 1999-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen
WO2000008195A1 (de) * 1998-07-31 2000-02-17 Dornier Medtech Holding International Gmbh Verfahren und vorrichtung zum transfer von oligonukleotiden in die zelle

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2578505A (en) * 1948-03-02 1951-12-11 Sperry Prod Inc Supersonic agitation
US3406302A (en) * 1966-03-15 1968-10-15 Westinghouse Electric Corp Cylindrical magnetostrictive electromechanical transducer
DE2346649A1 (de) * 1973-09-17 1975-03-27 Ngk Spark Plug Co Ultraschallgeber
US4369100A (en) * 1977-09-27 1983-01-18 Sawyer Harold T Method for enhancing chemical reactions
US5395592A (en) * 1993-10-04 1995-03-07 Bolleman; Brent Acoustic liquid processing device
CA2238951A1 (fr) * 1998-05-26 1999-11-26 Les Technologies Sonomax Inc. Reacteur a cavitation acoustique pour le traitement des materiaux

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3821354A1 (de) * 1987-07-10 1989-07-06 Berlin Kosmetik Veb Verfahren und vorrichtung zur herstellung bioaktiver suspensionen
WO1989002464A1 (en) * 1987-09-07 1989-03-23 Amersham International Plc Modifying living cells
EP0326701A2 (de) * 1988-02-04 1989-08-09 Dornier Medizintechnik Gmbh Piezoelektrische Stosswellenquelle
WO1999058637A2 (de) * 1998-05-07 1999-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen
WO2000008195A1 (de) * 1998-07-31 2000-02-17 Dornier Medtech Holding International Gmbh Verfahren und vorrichtung zum transfer von oligonukleotiden in die zelle

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HUBER PETER E ET AL: "A comparison of shock wave and sinusoidal-focused ultrasound-induced localized transfection of HeLa cells.", ULTRASOUND IN MEDICINE AND BIOLOGY, vol. 25, no. 9, November 1999 (1999-11-01), pages 1451 - 1457, XP001005203, ISSN: 0301-5629 *
MILLER DOUGLAS L ET AL: "Sonoporation of cultured cells in the rotating tube exposure system.", ULTRASOUND IN MEDICINE AND BIOLOGY, vol. 25, no. 1, January 1999 (1999-01-01), pages 143 - 149, XP001005257, ISSN: 0301-5629 *

Also Published As

Publication number Publication date
US20030017578A1 (en) 2003-01-23
JP2003533974A (ja) 2003-11-18
WO2001048181A2 (de) 2001-07-05
CA2397271A1 (en) 2001-07-05
EP1244770A2 (de) 2002-10-02

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