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WO2000065022A1 - Instrument pour tester un echantillon et instrument pour epreuve de contamination par frottis - Google Patents

Instrument pour tester un echantillon et instrument pour epreuve de contamination par frottis Download PDF

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Publication number
WO2000065022A1
WO2000065022A1 PCT/JP2000/002478 JP0002478W WO0065022A1 WO 2000065022 A1 WO2000065022 A1 WO 2000065022A1 JP 0002478 W JP0002478 W JP 0002478W WO 0065022 A1 WO0065022 A1 WO 0065022A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
atp
extractant
wiping
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2000/002478
Other languages
English (en)
Japanese (ja)
Inventor
Yasuhiro Harada
Seiji Murakami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Priority to AU36801/00A priority Critical patent/AU3680100A/en
Publication of WO2000065022A1 publication Critical patent/WO2000065022A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence

Definitions

  • the present invention relates to a device for collecting biological cells in a liquid or on a solid surface, removing free adelicin triphosphate (hereinafter abbreviated as free ATP), and measuring the amount thereof by a luminescence reaction.
  • free ATP free adelicin triphosphate
  • Measurement of Escherichia coli, yeast, lactic acid bacteria, and other biological cells is very important in fields such as food hygiene, biotechnology, clinical testing, medicine, ultrapure water, and the environment.
  • biological cells are measured using a hemocytometer under a microscope (microscope method), turbidity measurement method, weighing method, packed 'volume (packed volume) ⁇ ] method, and colony counting method ( Hereinafter, the pour culture method) is known.
  • the microscopic method, the turbidity measuring method, the gravimetric method, the packed volume method, and the like have a problem that the sensitivity is low and it is impossible to distinguish dead cells from viable cells.
  • the mixed culture method requires the cultivation of biological cells, so measurement usually takes one day or more, and is not suitable for obtaining rapid results.
  • a method for measuring the concentration of microorganisms that satisfies the above requirements, there is known a method for measuring ATP, which is always present in living microorganisms, using a bioluminescence method.
  • This method A sample containing biological cells is contacted with an extractant containing tricloacetic acid (TCA), Tris buffer, ethanol, or a lytic enzyme to extract intracellular ATP outside the cell, and then extract The resulting ATP is contacted with a luminescent reagent containing luciferin, a substrate for firefly luminescence, and luciferase, an enzyme. Then, luciferin, luciferase, and ATP (bioluminescence is generated by an enzymatic reaction, and the amount of generated light is measured to measure intracellular ATP to measure biological cells.
  • TCA tricloacetic acid
  • Tris buffer Tris buffer
  • ethanol ethanol
  • a lytic enzyme to extract intracellular ATP outside the cell
  • resulting ATP is
  • ATP is originally contained in the cells of all living organisms, albeit in varying amounts, and exists as so-called somatic cells not only in microorganisms, but also in single-celled organisms, and in cells of animal and plant tissues. In addition, free (free) ATP exists around living cells.
  • the present invention relates to the use of adenosine phosphate deaminase alone or to the group consisting of avilase, allyl phosphatase, acid phosphatase, hexokinase, and adenosine triphosphatase together with the adenosine phosphate deaminase.
  • a first concept of the present invention (according to the above, a sample testing instrument for measuring the amount of biological cells by collecting biological cells from a sample and causing the cells to emit light, comprising: a free ATP scavenger; There is provided a sample testing instrument comprising: an extractant for extracting cell-derived ATP; and a luminescent reagent for causing the ATP to act on the extracted ATP to emit light.
  • the sample testing instrument has a test tube shape, a measurement container containing the luminescent reagent, a tube shape, and a sealing material at each of the upper and lower openings.
  • a breaker for breaking the sealing material is provided at a lower part inside the ATP erasing agent container and the extracting agent container. This breaker breaks through the sealing material by being bent downward from below the swab. In order to easily break the sealing material, for example, according to the embodiment, it is preferable to form a projection projecting downward on the lower surface of the tip of the breaker.
  • a breaking force for breaking a seal material located above the ATP erasing agent container is further provided at an upper end portion of the measurement container.
  • a wiping test instrument for measuring the amount of biological cells by collecting biological cells from a specimen and causing the cells to emit light.
  • a specimen wiping tool having a detached main body, a holding member detachably inserted into an upper end opening of the main body, a cotton swab held by the holding member, a free ATP eliminating agent, and a biological cell-derived ATP.
  • a luminous reagent containing a luminescent reagent for causing the ATP to act on the extracted ATP to emit light.
  • the present invention provides a wiping inspection instrument which is fitted in a lower end opening of a wiping inspection device.
  • a biological cell is collected from a specimen and luminescent.
  • a wiping test instrument for measuring the amount of biological cells by applying a breaker breaking member having a ⁇ -square shape at the lower end thereof, a tubular body having upper and lower openings, and an upper opening of the body.
  • a sample wiping tool having a holding member detachably inserted into the part, a cotton swab held by the holding member, an ATP erasing agent container and an extractant container provided at a lower portion in the main body, and a test tube
  • a measuring container having a shape and being vertically movably inserted into an opening at the lower end of the main body.
  • FIG. 1 is a sectional view of a wiping inspection instrument according to a first embodiment of the present invention.
  • FIG. 2 is an enlarged cross-sectional view of the sample testing instrument shown in FIG.
  • FIG. 3 is an enlarged cross-sectional view taken along line m-m in FIG.
  • FIG. 4 is an enlarged sectional view taken along line IV-IV in FIG.
  • FIG. 5 is a cross-sectional view showing another embodiment of the sample testing instrument.
  • FIG. 6 is a sectional view showing another embodiment of the wiping inspection instrument.
  • FIG. 7 is a cross-sectional view showing still another embodiment of the sample testing instrument. BEST MODE FOR CARRYING OUT THE INVENTION
  • free ATP is decomposed with a free ATP scavenger from a sample collected by wiping with a cotton swab or the like, and then ATP derived from a biological cell is extracted with an extractant. Then, a luminescent reagent is allowed to act on the extracted ATP derived from biological cells, and ATP, that is, biological cells are detected based on the amount of luminescence.
  • Examples of the free ATP scavenger include reagents obtained by using a single or a combination of avirase, alkaline phosphatase, acid phosphatase, hexokinase, adenosine triphosphatase, and adenosine phosphate deaminase.
  • ATP extraction reagent for example, a mixed solution of ethanol and ammonia, methanol, ethanol, a surfactant, trichloroacetic acid, perchloric acid and the like can be used.
  • Detergents have high ATP extraction efficiency, so ATP extraction Suitable as a reagent.
  • Surfactants include anionic surfactants (eg, sodium dodecyl sulfate (SDS), potassium lauryl sulfate, sodium monolauroyl phosphate, sodium alkyl benzene sulfonate), surfactants (For example, benzalkonium chloride (BAC), benzatonium chloride (BZC), cetylpyridinium chloride, cetyltrimethylammonium bromide, myristyl dimethyl benzyl benzylammonium), amphoteric 1) Surfactants (for example, Twittergent Detergent 3-08,3-10,3-12,3-14,3-16, Tego), nonionic surfactants (for example, Tween20, 60,80, Span 60,80, Triton X-45, X-100, poly (xylene ethylene ether), poly (xylene ethylene lauryl ether) can be used.
  • anionic surfactants eg, sodium dodecyl sulf
  • Examples of the luminescent reagent include a reagent containing luciferin which is a substrate of firefly luminescence and luciferase which is an enzyme.
  • a wiping inspection instrument 1 includes a main body 2 mainly formed in a tubular shape with upper and lower openings, and an attaching / detaching itself through an upper opening 3 of the main body 2.
  • the apparatus includes a sample wiping tool 4 to be inserted, and a biological cell sample test device 6 fitted into the lower opening 5 of the main body 2.
  • the specimen wiping tool 4 includes a swab 9 and a holding member 10 for holding the swab 9.
  • the cotton swab 9 has a rod-shaped cotton shaft 7 and a cotton portion 8 formed in an oval shape at the lower end of the cotton shaft 7.
  • the specimen 4 is removed from the main body 2 and the surface of the specimen is wiped with a cotton swab 9 to collect biological cells.
  • the holding member 10 includes a lower portion 11 having a lower diameter and a larger portion 12 having an upper portion.
  • the diameter of the small portion 11 is such that the holding member 10 stops at an arbitrary position of the main body 2 when the holding member 10 is inserted into the main body 2.
  • Tool 4 acts as a stop to prevent further lowering.
  • a plurality of linear projection members 15 extending in the axial longitudinal direction are provided on the upper inner wall surface of the main body 2.
  • the distal end 16 of these projection members 15 and the outer peripheral edge of the flange-shaped member 17 provided at the middle part of the holding member 10 are in sliding contact with each other, and the movement of the holding member 10 relative to the main body 2 is made smooth. I have.
  • FIG. 2 shows the sample test device 6.
  • This sample test device 6 A measuring container 20 formed in the shape of a test tube, an ATP erasing agent container 22 fitted to the opening 21 of the measuring container 20 and positioned above, and a lower portion of the ATP erasing agent container 22 Extraction agent container 23.
  • the sample testing instrument 6 is pushed into the main body 2 until it comes into contact with a stepped portion 24 formed at the upper portion of the lower opening 5 of the main body 2.
  • the ATP erasing agent container 22 has a cylindrical shape, and has a sealed chamber 27 formed by sticking sealing materials 25 and 26 to upper and lower openings. In the closed chamber 27, a free ATP removing agent 28 for removing free ATP attached to the cotton portion 8 is stored.
  • the ATP erasing agent container 22 has a breaker 29 at the lower portion inside.
  • the breaker 29 is composed of, for example, six breaker pieces 31 each having an arrow shape extending from the inner wall surface of the ATP eraser container 22 toward the center as shown in FIG.
  • the tip of each breaker piece 31 has a projection 30 projecting downward.
  • a breaker 32 having a structure similar to that of the breaker 29 described above is provided near the step portion 24 of the main body 2.
  • the breaker 32 penetrates the seal material 25 provided at the upper opening of the ATP eraser container 22 by the downward movement of the swab 9.
  • the breaker 29 of the ATP removing agent container 22 removes the surface of the cotton portion 8 provided at the tip of the cotton swab 9 to remove free ATP attached to the cotton portion 8 to the free ATP removing agent. 2. Effectively migrate to 8.
  • the swab 9 receives a slight downward movement resistance due to the breaker 29, but this resistance keeps the contact time between the free ATP scavenger 28 and the cotton section 8 longer, and causes the cotton section 8 to adhere to the cotton section 8. Transfer of free ATP to free ATP scavenger 28 is effective. Further, in order to prolong the contact time between the free ATP eliminating agent 28 and the cotton portion 8, the downward movement of the cotton swab 9 may be artificially stopped.
  • the structure of the extractant container 23 is the same as that of the ATP scavenger container 22 described above. You That is, the extractant container 23 has a cylindrical shape, and its upper and lower openings are sealed with sealants 33 and 34 to have a closed chamber 35.
  • the closed chamber 35 contains an extractant for extracting ATP from biological cells collected with a cotton swab 9.
  • the extractant container 23 has a breaker 37 at its lower part.
  • the breaker 37 has the same structure as the shake force 29 provided in the ATP eraser container 22.
  • the lower movement of the swab 9 causes the breaker 37 to bend downward and break through the sealing material 34.
  • the breaker 37 removes the surface of the cotton portion 8.
  • the closed chamber 38 is provided in the measurement container 20. It is formed. A luminescent reagent 39 is stored in the closed chamber 38.
  • the upper end surface 41 of the ATP eliminator container 22 located above the extractant container 23 is measured. It is preferable that the upper end surface 42 of the container 20 be flush with the upper surface 42, because the size of the sample testing instrument 6 can be reduced. Furthermore, the top surface of the measuring container 20 is sealed with a sealing material 25.
  • the opening of the sample testing instrument 6 is completely sealed, and when the sample testing instrument 6 is distributed independently as described later, it is necessary to prevent invasion of various bacteria from outside. It is useful and becomes a measurement container containing three types of reagents.
  • breakers 29, 32, and 37 are not necessarily provided if the sealing material 25, 26, 33, or 34 is easily broken by the swab 9, and should be provided as necessary. do it.
  • the sample wiping device 4 is provided in a state where it is inserted partway into the main body 2.
  • Luminescent reagent 39 is enclosed in measurement container 20
  • free ATP quencher 28 is enclosed in ATP quencher container 22
  • extractant 36 is enclosed in extractant container 23. It is.
  • the specimen wiping tool 4 is pulled out from the main body 2, and the specimen surface is wiped with the cotton part 8 provided at the tip of the swab 9, and the biological cells are collected.
  • the specimen wiping tool 4 from which the biological cells have been collected is inserted into the main body 2 again. Then, the sample wiping tool 4, that is, the cotton swab 9 is pushed down, and the breaker 3 2 (the seal material 25 shown in FIG. 2 is broken, and the cotton portion 8 is immersed in the free ATP scavenger 28.
  • the cotton swab 9 receives the pushing-down resistance by the breaker 29, but detects this pushing-down resistance and removes the free ATP without breaking the sealing material 26 by the instruction of the indicator (not shown) provided in the main body 2.
  • the cotton portion 8 is kept soaked in the free ATP scavenger 28 until the breaker 32 is opened by the cotton swab 9 into a petal shape. Since there is a step of removing with the tip of 32, the free ATP attached to the surface of the cotton portion 8 is efficiently transferred to the free ATP scavenger 28.
  • the sample wiping tool 14 is pushed down until the step 13 of the holding member 10 shown in FIG. 1 and the upper end 14 of the main body 2 come into contact with each other.
  • the sealing material 34 is broken by the breaking force 37, and the extractant 36 containing biological cells falls into the measuring container 20.
  • the luminescent reagent 39 and the extracting agent 36 come into contact, and the action of the luminescent reagent 39 causes a luminescent reaction to detect biological cells. .
  • the wiping test device 1 As described above, the wiping test device 1 according to the first embodiment has been described.
  • the sample test device 6 alone can be used as a test device. That is, the swab may be a commercially available swab, and after wiping the sample with the swab, piercing from the upper end of the sample test device 6 and tearing off the sealing materials 25, 26, 33, and 34 sequentially, as described above. If the procedure is performed as described above, the living cells of the specimen can be inspected.
  • the sample testing instrument 6 shown in FIG. 2 may be used, and as shown in FIG. The sample testing instrument 6 provided with 2 may be used.
  • the wiping inspection instrument 1a according to the second embodiment is configured such that the measurement container 20a is vertically movable with respect to the main body 2a, and the ATP eraser container 22 and the extractant container 23 are connected to the main body 2a. This is an example of insertion on the step portion 24 of FIG.
  • the opening of the measuring container 20a is sealed with a sealing material 43, and the luminescent reagent 39 is stored inside the measuring container 20a.
  • the main body 2 integrally has a pipe-shaped seal breaking member 44 whose tip is formed in a rectangular shape at the lower part of the step portion 24. The seal breaking member 44 pushes up the measuring container 20a to break the sealing material 43 of the measuring container 20a and open the measuring container 20a.
  • a breaker 32a, an ATP eliminator container 22 sealed with a free ATP eliminator 28, and an extractant container 23 sealed with an extractant 36 are sequentially provided from above. ing.
  • the free ATP is erased by moving down the swab 9, and then the ATP extraction and the elimination reaction are stopped by the living cells, and finally the sealing material 3 of the extractant container 23 4 is broken.
  • the extractant 36 containing ATP derived from biological cells falls and flows into the measurement container 20a through the communication hole 45 of the seal breaking member 44. Then, the extractant 36 containing ATP comes into contact with the luminescent reagent 39, and the biological cells are detected by the luminescent reaction.
  • FIG. 7 shows another embodiment of the sample testing instrument 6. This embodiment shows an example in which the opening 21 is sealed with a sealing material 25a larger than the outer diameter of the container 20 at the opening 21 of the measuring container 20 of the specimen inspection instrument 6. ing.
  • the portion 25 b protruding from the outer diameter of the measuring container 20 is picked up with a finger, and the sealing material 25 a is peeled off. Can be omitted. Furthermore, a durable material can be used as the sealing material 25a, and the quality is improved as a single product. Industrial applicability
  • free ATP can be eliminated and only the target biological cells can be measured. Useful as a test tool.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un instrument utilisé pour tester un échantillon. Des cellules vitales sont prélevées d'un échantillon donné, l'ATP provenant de ces cellules étant mesuré avec grande précision. Cet instrument comporte un contenant de mesure en forme de tube de test, un contenant tubulaire destiné à contenir un éliminateur d'ATP fixé sur la partie supérieure du contenant de mesure, au niveau de son ouverture supérieure, et un contenant tubulaire destiné à contenir un agent d'extraction fixé sous le contenant de l'éliminateur d'ATP, situé dans l'ouverture du contenant de mesure. Un réactif phosphorescent est introduit dans le contenant de mesure. Le contenant de l'éliminateur d'ATP comporte un matériau d'étanchéité collé à la fois à l'ouverture supérieure et inférieure, et contient un éliminateur d'ATP libre. Le conteneur de l'agent d'extraction comporte un matériau d'étanchéité collé à la fois à l'ouverture supérieure et inférieure, et contient un agent d'extraction permettant d'extraire l'ATP décrit plus haut.
PCT/JP2000/002478 1999-04-22 2000-04-17 Instrument pour tester un echantillon et instrument pour epreuve de contamination par frottis Ceased WO2000065022A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU36801/00A AU3680100A (en) 1999-04-22 2000-04-17 Instrument for testing specimen and instrument for wipe test

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11507799 1999-04-22
JP11/115077 1999-04-22

Publications (1)

Publication Number Publication Date
WO2000065022A1 true WO2000065022A1 (fr) 2000-11-02

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PCT/JP2000/002478 Ceased WO2000065022A1 (fr) 1999-04-22 2000-04-17 Instrument pour tester un echantillon et instrument pour epreuve de contamination par frottis

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WO (1) WO2000065022A1 (fr)

Cited By (21)

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WO2002085240A3 (fr) * 2001-04-20 2004-03-11 3M Innovative Properties Co Recipient pour compositions a plusieurs composants
WO2007112981A1 (fr) * 2006-03-30 2007-10-11 S & C Polymer Silicon- Und Composite Spezialitäten Gmbh Système d'emballage à usage unique permettant le stockage et la distribution de matières à plusieurs composants
WO2010129726A1 (fr) * 2009-05-06 2010-11-11 3M Innovative Properties Company Articles à matrice comprenant un agent d'extraction cellulaire et procédés de biodétection associés
WO2010129727A1 (fr) * 2009-05-06 2010-11-11 3M Innovative Properties Company Substrats enduits comprenant un agent extracteur cellulaire et procédés de biodétection associés
WO2010129728A3 (fr) * 2009-05-06 2011-04-28 3M Innovative Properties Company Articles avec structures en couches contenant un agent d'extraction cellulaire et méthodes de biodétection associées
WO2012118392A1 (fr) * 2011-03-01 2012-09-07 Infogene Lda. Dispositif portatif pour le stockage, le transport et la récupération d'une matière biologique
DE102013000922A1 (de) * 2013-01-19 2014-07-24 Dräger Safety AG & Co. KGaA Vorrichtung zur schnellen Aufnahme und Abgabe von Proben, System mit einem Probenehmer und dessen Verwendung
CN105527257A (zh) * 2014-10-22 2016-04-27 肖特玻璃科技(苏州)有限公司 测定玻璃抗菌性的方法和装置及其用途
EP3209366A4 (fr) * 2014-10-22 2018-07-18 Ibis Biosciences, Inc. Appareils pour distribuer un fluide de manière stérile
WO2019181400A1 (fr) * 2018-03-22 2019-09-26 富士フイルム株式会社 Méthode de traitement d'échantillon et récipient pour traitement d'échantillon
US20200278368A1 (en) * 2017-09-27 2020-09-03 Axxin Pty Ltd Diagnostic test system and method
CN112119153A (zh) * 2018-05-29 2020-12-22 株式会社日立高新技术 细胞检测装置和细胞检测方法
CN113189075A (zh) * 2021-05-12 2021-07-30 浙江省疾病预防控制中心 一种基于atp的冷链食品外包装清洁度检测方法
CN113348238A (zh) * 2019-02-15 2021-09-03 株式会社日立高新技术 细胞检测装置以及细胞检测方法
WO2021198476A1 (fr) * 2020-04-02 2021-10-07 Diascreen Gmbh Diagnostics de point d'intervention contrôlés par pression
WO2021204900A1 (fr) * 2020-04-07 2021-10-14 Midge Medical Gmbh Système et dispositif pour analyser un échantillon
WO2021204901A1 (fr) * 2020-04-07 2021-10-14 Midge Medical Gmbh Ensemble de chambres contenant des réactifs
EP3995208A1 (fr) * 2020-11-09 2022-05-11 midge medical GmbH Ensemble de chambres contenant des réactifs
EP4015080A1 (fr) * 2020-12-17 2022-06-22 PHILMEDI Co., Ltd. Kit tout-en-un pour diagnostic moléculaire sur site et procédé de diagnostic moléculaire l'utilisant
US11662345B2 (en) 2018-01-24 2023-05-30 Homedicus Gmbh Testing assembly and testing device for lateral flow assay
US20250064339A1 (en) * 2018-03-15 2025-02-27 Biolum Sciences, Llc Sensor devices and systems for monitoring markers in breath

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JPH0759555A (ja) * 1993-08-26 1995-03-07 Nippon Health Sci Kk 包装体
US5891702A (en) * 1995-12-28 1999-04-06 Kikkoman Corporation ATP eliminator and the process for determining biological cells

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Publication number Priority date Publication date Assignee Title
JPH0759555A (ja) * 1993-08-26 1995-03-07 Nippon Health Sci Kk 包装体
US5891702A (en) * 1995-12-28 1999-04-06 Kikkoman Corporation ATP eliminator and the process for determining biological cells

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085240A3 (fr) * 2001-04-20 2004-03-11 3M Innovative Properties Co Recipient pour compositions a plusieurs composants
WO2007112981A1 (fr) * 2006-03-30 2007-10-11 S & C Polymer Silicon- Und Composite Spezialitäten Gmbh Système d'emballage à usage unique permettant le stockage et la distribution de matières à plusieurs composants
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WO2010129727A1 (fr) * 2009-05-06 2010-11-11 3M Innovative Properties Company Substrats enduits comprenant un agent extracteur cellulaire et procédés de biodétection associés
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