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WO1999063054A3 - Mutant forms of the al2 gene of geminiviruses - Google Patents

Mutant forms of the al2 gene of geminiviruses Download PDF

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Publication number
WO1999063054A3
WO1999063054A3 PCT/US1999/012680 US9912680W WO9963054A3 WO 1999063054 A3 WO1999063054 A3 WO 1999063054A3 US 9912680 W US9912680 W US 9912680W WO 9963054 A3 WO9963054 A3 WO 9963054A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
amino acid
region
domain mutant
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/012680
Other languages
French (fr)
Other versions
WO1999063054A2 (en
Inventor
David Bisaro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ohio State University Research Foundation
Original Assignee
Ohio State University Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio State University Research Foundation filed Critical Ohio State University Research Foundation
Priority to AU43356/99A priority Critical patent/AU4335699A/en
Publication of WO1999063054A2 publication Critical patent/WO1999063054A2/en
Publication of WO1999063054A3 publication Critical patent/WO1999063054A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Isolated double domain mutant AL2 genes of a geminivirus are provided. The double domain AL2 mutant gene comprises at least one mutation in the 3' region of the AL2 gene, i.e., the region which encodes about 30 amino acids extending from about amino acid 100 to about amino acid 129 of the wild-type AL2 gene product and at least one mutation in the central region of the AL2 gene, i.e., the region which encodes about 20 amino acids extending from about amino acid 23 to about amino acid 43 of the wild-type AL2 gene product. Isolated single domain mutant AL2 genes of a geminivirus are also provided. The single domain mutant AL2 gene comprises at least one mutation in the 3' region of the AL2 gene, the region which encodes about 30 amino acids extending from about amino acid 100 to about amino acid 129 of the wild-type AL2 gene product. The present invention also relates to vectors comprising a double domain mutant AL2 gene and to vectors comprising a single domain mutant AL2 gene. The present invention also relates to transgenic plants comprising a double domain mutant AL2 gene and to transgenic plants comprising a single domain mutant AL2 gene.
PCT/US1999/012680 1998-06-05 1999-06-04 Mutant forms of the al2 gene of geminiviruses Ceased WO1999063054A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU43356/99A AU4335699A (en) 1998-06-05 1999-06-04 Mutant forms of the al2 gene of geminiviruses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9270598A 1998-06-05 1998-06-05
US09/092,705 1998-06-05

Publications (2)

Publication Number Publication Date
WO1999063054A2 WO1999063054A2 (en) 1999-12-09
WO1999063054A3 true WO1999063054A3 (en) 2000-06-15

Family

ID=22234668

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/012680 Ceased WO1999063054A2 (en) 1998-06-05 1999-06-04 Mutant forms of the al2 gene of geminiviruses

Country Status (2)

Country Link
AU (1) AU4335699A (en)
WO (1) WO1999063054A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2806095A1 (en) * 2000-03-10 2001-09-14 Gentech New polynucleotides for producing transgenic plants resistant to geminivirus infection comprising polynucleotides encoding proteins which interact with at least one of the products of the geminivirus genome
CN102640642B (en) * 2012-04-25 2013-11-06 浙江大学 Method for pierce-inoculating geminivirus infectious clone-containing solid colony to plant, and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GILLETTE ET AL.: "Genetic Determinants of Host-Specificity in Bipartite Geminivirus DNA A Components", VIROLOGY, vol. 251, 1998, pages 361 - 369, XP002927219 *
HARTITZ ET AL.: "The Tomato Golden Mosaic Virus Transactivator (TrAP) is a Single-Stranded DNA and Zinc-Binding Phosphoprotein with an Acidic Activation Domain", VIROLOGY, vol. 263, 1999, pages 1 - 14, XP002927222 *
SUNG ET AL.: "Mutational Analysis of Potato Yellow Mosaic Geminivirus", J. GEN. VIROL., vol. 76, 1995, pages 1773 - 1780, XP002927220 *
SUNTER ET AL.: "Regulation of a Geminivirus Coat Protein Promoter by AL2 Protein (TraP): Evidence for Activation and Derepression Mechanisms", VIROLOGY, vol. 232, 1997, pages 269 - 280, XP002927221 *

Also Published As

Publication number Publication date
AU4335699A (en) 1999-12-20
WO1999063054A2 (en) 1999-12-09

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