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WO1999033960A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999033960A3
WO1999033960A3 PCT/US1998/027040 US9827040W WO9933960A3 WO 1999033960 A3 WO1999033960 A3 WO 1999033960A3 US 9827040 W US9827040 W US 9827040W WO 9933960 A3 WO9933960 A3 WO 9933960A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Application number
PCT/US1998/027040
Other languages
French (fr)
Other versions
WO1999033960A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/554,999 priority Critical patent/US6465186B1/en
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to AU18327/99A priority patent/AU1832799A/en
Priority to EP98963276A priority patent/EP1042487A2/en
Publication of WO1999033960A2 publication Critical patent/WO1999033960A2/en
Publication of WO1999033960A3 publication Critical patent/WO1999033960A3/en
Priority to US10/188,586 priority patent/US6794179B2/en
Priority to US10/926,197 priority patent/US7070986B2/en
Priority to US10/925,708 priority patent/US7070990B2/en
Priority to US10/925,821 priority patent/US7078372B2/en
Priority to US10/925,652 priority patent/US7070819B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/027040 1997-12-30 1998-12-17 Proteases from gram positive organisms WO1999033960A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US09/554,999 US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
AU18327/99A AU1832799A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98963276A EP1042487A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US10/188,586 US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms
US10/926,197 US7070986B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/925,708 US7070990B2 (en) 1997-12-30 2004-08-24 Proteases from GRAM positive organisms
US10/925,821 US7078372B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/925,652 US7070819B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727471.6A GB9727471D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727471.6 1997-12-30

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/554,999 Continuation US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
US09554999 A-371-Of-International 1998-12-17
US10/188,586 Division US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999033960A2 WO1999033960A2 (en) 1999-07-08
WO1999033960A3 true WO1999033960A3 (en) 2000-03-02

Family

ID=10824314

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027040 WO1999033960A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042487A2 (en)
AU (1) AU1832799A (en)
GB (1) GB9727471D0 (en)
WO (1) WO1999033960A2 (en)

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US6592764B1 (en) * 1997-12-09 2003-07-15 The Regents Of The University Of California Block copolymer processing for mesostructured inorganic oxide materials
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US20060121565A1 (en) * 2002-09-20 2006-06-08 Cymip A/S Methods for increasing the production of a recombinant polypeptide from a host cell
DK3080262T3 (en) 2013-12-13 2019-05-06 Danisco Us Inc SERIN PROTEAS OF BACILLUS SPECIES
DK3080263T3 (en) 2013-12-13 2019-10-07 Danisco Us Inc SERIN PROTEASES OF BACILLUS GIBSONII-CLADE
EP3083704B1 (en) 2013-12-16 2022-08-17 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
KR102410391B1 (en) 2013-12-18 2022-06-16 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. Cationic poly alpha-1,3-glucan ethers
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
ES2734726T3 (en) 2014-03-11 2019-12-11 Du Pont Oxidized alpha-1,2-glucan poly as a detergent booster
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3919599A1 (en) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
WO2016061438A1 (en) 2014-10-17 2016-04-21 Danisco Us Inc. Serine proteases of bacillus species
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016069548A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
DK3212662T3 (en) 2014-10-27 2020-07-20 Danisco Us Inc serine proteases
EP3224357A1 (en) 2014-10-27 2017-10-04 Danisco US Inc. Serine proteases of bacillus species
EP3237631A1 (en) 2014-12-23 2017-11-01 E. I. du Pont de Nemours and Company Enzymatically produced cellulose
EP4219704A3 (en) 2015-05-13 2023-08-23 Danisco US Inc Aprl-clade protease variants and uses thereof
WO2016205755A1 (en) 2015-06-17 2016-12-22 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
US20180320158A1 (en) 2015-11-05 2018-11-08 Danisco Us Inc. Paenibacillus and bacillus spp. mannanases
US20190153417A1 (en) 2015-11-05 2019-05-23 Danisco Us Inc Paenibacillus sp. mannanases
WO2017083229A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
WO2017106676A1 (en) 2015-12-18 2017-06-22 Danisco Us Inc Polypeptides with endoglucanase activity and uses thereof
JP7609549B2 (en) 2016-05-31 2025-01-07 ダニスコ・ユーエス・インク Protease variants and uses thereof
JP7152319B2 (en) 2016-06-17 2022-10-12 ダニスコ・ユーエス・インク Protease variants and uses thereof
US20190264138A1 (en) 2016-11-07 2019-08-29 Danisco Us Inc. Laundry detergent composition
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
WO2018118917A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Protease variants and uses thereof
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
CN111373039A (en) 2017-11-29 2020-07-03 丹尼斯科美国公司 Subtilisin variants having improved stability
EP3810767A1 (en) 2018-06-19 2021-04-28 Danisco US Inc. Subtilisin variants
US20210363470A1 (en) 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
EP3856882A1 (en) 2018-09-27 2021-08-04 Danisco US Inc. Compositions for medical instrument cleaning
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
CN114174504A (en) 2019-05-24 2022-03-11 丹尼斯科美国公司 Subtilisin variants and methods of use
EP3980517A1 (en) 2019-06-06 2022-04-13 Danisco US Inc. Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
US20240117275A1 (en) 2021-01-29 2024-04-11 Danisco Us Inc. Compositions for cleaning and methods related thereto
CN117616120A (en) 2021-06-30 2024-02-27 丹尼斯科美国公司 Variant lipases and uses thereof
CN117916354A (en) 2021-09-03 2024-04-19 丹尼斯科美国公司 Laundry detergent compositions for cleaning
EP4448749A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
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US20250163396A1 (en) 2022-03-01 2025-05-22 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
EP4544016A1 (en) 2022-06-21 2025-04-30 Danisco US Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
EP4581138A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Subtilisin variants and methods related thereto
CN119855892A (en) 2022-09-02 2025-04-18 丹尼斯科美国公司 Detergent compositions and methods relating thereto
EP4581137A1 (en) 2022-09-02 2025-07-09 Danisco Us Inc Mannanase variants and methods of use
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024186819A1 (en) 2023-03-06 2024-09-12 Danisco Us Inc. Subtilisin variants and methods of use
WO2024191711A1 (en) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Citations (1)

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Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002115981, Database accession no. Z99112 *
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), XP002115982, Database accession no. G69885 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
GB9727471D0 (en) 1998-02-25
WO1999033960A2 (en) 1999-07-08
AU1832799A (en) 1999-07-19
EP1042487A2 (en) 2000-10-11

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