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WO1998038285A3 - Carboxypeptidase modifie - Google Patents

Carboxypeptidase modifie Download PDF

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Publication number
WO1998038285A3
WO1998038285A3 PCT/IB1998/000517 IB9800517W WO9838285A3 WO 1998038285 A3 WO1998038285 A3 WO 1998038285A3 IB 9800517 W IB9800517 W IB 9800517W WO 9838285 A3 WO9838285 A3 WO 9838285A3
Authority
WO
WIPO (PCT)
Prior art keywords
carboxypeptidase
transamidation
methods
amino acid
subsite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB1998/000517
Other languages
English (en)
Other versions
WO1998038285A2 (fr
Inventor
Uffe Mortensen
Kjeld Olesen
Henning Stennicke
Steen B Soerensen
Klaus Breddam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carlsberg Laboratory
Original Assignee
Carlsberg Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlsberg Laboratory filed Critical Carlsberg Laboratory
Priority to JP53746298A priority Critical patent/JP2001513638A/ja
Priority to EP98910909A priority patent/EP0966526A2/fr
Priority to AU65133/98A priority patent/AU740742B2/en
Priority to CA002281879A priority patent/CA2281879A1/fr
Publication of WO1998038285A2 publication Critical patent/WO1998038285A2/fr
Publication of WO1998038285A3 publication Critical patent/WO1998038285A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé de transmission d'un substrat peptidique présentant un résidu d'acide aminé P1 au moyen d'une chaîne latérale à charge positive. Selon l'invention, le carboxypeptidase Y est modifié pour remplacer au moins un acide aminé présentant une chaîne latérale à charge négative dans un site secondaire S1. En outre, le carboxypeptidase Y modifié peut inclure des résidus d'acide aminé substitué dans un site secondaire S1', S2 et/ou S3 pour incorporer un substrat peptidique spécifique.
PCT/IB1998/000517 1997-02-28 1998-02-27 Carboxypeptidase modifie Ceased WO1998038285A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP53746298A JP2001513638A (ja) 1997-02-28 1998-02-27 修飾されたカルボキシペプチダーゼ
EP98910909A EP0966526A2 (fr) 1997-02-28 1998-02-27 Carboxypeptidase modifie
AU65133/98A AU740742B2 (en) 1997-02-28 1998-02-27 Modified carboxypeptidase
CA002281879A CA2281879A1 (fr) 1997-02-28 1998-02-27 Carboxypeptidase modifie

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/807,263 1997-02-28
US08/807,263 US5985627A (en) 1997-02-28 1997-02-28 Modified carboxypeptidase

Publications (2)

Publication Number Publication Date
WO1998038285A2 WO1998038285A2 (fr) 1998-09-03
WO1998038285A3 true WO1998038285A3 (fr) 1999-01-07

Family

ID=25195962

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1998/000517 Ceased WO1998038285A2 (fr) 1997-02-28 1998-02-27 Carboxypeptidase modifie

Country Status (7)

Country Link
US (2) US5985627A (fr)
EP (1) EP0966526A2 (fr)
JP (1) JP2001513638A (fr)
CN (1) CN1252836A (fr)
AU (1) AU740742B2 (fr)
CA (1) CA2281879A1 (fr)
WO (1) WO1998038285A2 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9323429D0 (en) * 1993-11-12 1994-01-05 Wellcome Found Therapy
IL123271A0 (en) * 1995-08-16 1998-09-24 Zeneca Ltd Chemical compounds
DE10047857B4 (de) * 2000-09-27 2004-11-18 Universität Leipzig Verfahren zur selektiven biokatalytischen Modifizierung von Peptiden und Proteinen
AU2003240796A1 (en) * 2002-05-24 2003-12-12 Restoragen Inc. Peptide derivatization process
US7524813B2 (en) 2003-10-10 2009-04-28 Novo Nordisk Health Care Ag Selectively conjugated peptides and methods of making the same
WO2006015879A1 (fr) 2004-08-13 2006-02-16 Roche Diagniostics Gmbh Modification du c-terminal de polypeptides
WO2006035008A1 (fr) * 2004-09-27 2006-04-06 Merck Biosciences Ag Carboxypeptidase b recombinante
US20090105134A1 (en) 2005-02-10 2009-04-23 Novo Nordisk A/S C-Terminally Pegylated Growth Hormones
WO2009068617A1 (fr) 2007-11-30 2009-06-04 Boehringer Ingelheim International Gmbh Dérivés de 1,5-dihydro-pyrazolo(3,4-d)pyrimidin-4-one et leur utilisation en tant que modulateurs de pde9a pour le traitement de troubles du snc
UA105362C2 (en) 2008-04-02 2014-05-12 Бьорингер Ингельхайм Интернациональ Гмбх 1-heterocyclyl-1, 5-dihydro-pyrazolo [3, 4-d] pyrimidin-4-one derivatives and their use as pde9a modulators
MX2011000847A (es) 2008-08-06 2011-02-25 Novo Nordisk Healthcare Ag Proteinas conjugadas con eficacia prolongada in vivo.
JP5453431B2 (ja) 2008-09-08 2014-03-26 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング ピラゾロピリミジン及びｃｎｓ障害の治療のためのそれらの使用
RU2539797C2 (ru) 2009-01-22 2015-01-27 Ново Нордиск Хелс Кеа Аг Производное гормона роста человека с повышенной стабильностью к протеолитическому разрушению, способ получения такого производного, его применение, способ лечения и фармацевтическая композиция
BRPI1011533A2 (pt) 2009-03-31 2016-03-29 Boehringer Ingelheim Int derivados de 1-heterocicil-1 5- diidro-pirazolo [3,4--d] pirimidin-4-ona e seu uso como moduladores de pde9a.
CN102612376A (zh) 2009-08-06 2012-07-25 诺沃-诺迪斯克保健股份有限公司 具有延长的体内功效的生长激素
CA2787890C (fr) 2010-01-22 2020-01-14 Novo Nordisk Health Care Ag Hormones de croissance presentant une efficacite in vivo prolongee
AU2011208620B2 (en) 2010-01-22 2015-04-16 Novo Nordisk Health Care Ag Stable growth hormone compounds
JP5756176B2 (ja) 2010-08-12 2015-07-29 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング ６−シクロアルキル−１，５−ジヒドロ−ピラゾロ（３，４−ｄ）ピリミジ−４−オン誘導体及びｐｄｅ９ａ阻害剤としてのその使用
US8809345B2 (en) 2011-02-15 2014-08-19 Boehringer Ingelheim International Gmbh 6-cycloalkyl-pyrazolopyrimidinones for the treatment of CNS disorders
ES2841123T3 (es) 2013-04-05 2021-07-07 Formulacion Del Compuesto De La Hormona De Crecimiento Formulación del compuesto de la hormona de crecimiento

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020039A2 (fr) * 1993-10-28 1995-07-27 Calsberg A/S Proteases particularisees a activite de transacylation modifiee

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA801929B (en) * 1979-04-06 1981-11-25 Forenede Bryggerier As A process for enzymatic production of peptides
DK149824C (da) * 1982-01-22 1987-03-16 Carlsberg Biotechnology Ltd Fremgangsmaade til enzymatisk udskiftning af b-30 aminosyrer i insuliner
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
JPH07121222B2 (ja) * 1987-03-24 1995-12-25 寶酒造株式会社 化学修飾セリンプロテア−ゼ含有組成物及びその製造方法並びに用途
JP3362847B2 (ja) * 1990-08-09 2003-01-07 ジェネンテック インコーポレイテッド ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020039A2 (fr) * 1993-10-28 1995-07-27 Calsberg A/S Proteases particularisees a activite de transacylation modifiee

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OLESEN, K. ET AL.: "The activity of carboxypeptidase Y toward substrates with basic P1 amino acid residues ...", BIOCHEMISTRY, vol. 33, 1994, pages 11121 - 11126, XP002074788 *

Also Published As

Publication number Publication date
US20010010923A1 (en) 2001-08-02
JP2001513638A (ja) 2001-09-04
AU6513398A (en) 1998-09-18
EP0966526A2 (fr) 1999-12-29
AU740742B2 (en) 2001-11-15
CN1252836A (zh) 2000-05-10
CA2281879A1 (fr) 1998-09-03
US5985627A (en) 1999-11-16
WO1998038285A2 (fr) 1998-09-03

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