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WO1998002536A3 - Preparation of pichia methanolica auxotrophic mutants - Google Patents

Preparation of pichia methanolica auxotrophic mutants Download PDF

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Publication number
WO1998002536A3
WO1998002536A3 PCT/US1997/012582 US9712582W WO9802536A3 WO 1998002536 A3 WO1998002536 A3 WO 1998002536A3 US 9712582 W US9712582 W US 9712582W WO 9802536 A3 WO9802536 A3 WO 9802536A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
culturing
culture medium
methanolica
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/012582
Other languages
French (fr)
Other versions
WO1998002536A2 (en
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/703,808 external-priority patent/US5736383A/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to DE69735369T priority Critical patent/DE69735369T2/en
Priority to EP97936103A priority patent/EP0946734B1/en
Priority to JP50632398A priority patent/JP2002514049A/en
Priority to CA002261020A priority patent/CA2261020C/en
Priority to IL12807297A priority patent/IL128072A0/en
Priority to AU38856/97A priority patent/AU708572B2/en
Publication of WO1998002536A2 publication Critical patent/WO1998002536A2/en
Publication of WO1998002536A3 publication Critical patent/WO1998002536A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for preparing Pichia methanolica cells having auxotrophic mutations are disclosed. The methods comprise the steps of: (a) exposing P. methanolica cells to mutagenizing conditions; (b) culturing the cells from step (a) in a rich medium to allow mutations to become established and replicated in at least a portion of the cells; (c) culturing the cells from step (b) in a culture medium deficient in assimilable nitrogen to deplete cellular nitrogen stores; (d) culturing the cells from step (c) in a defined culture medium comprising an inorganic nitrogen source and an amount of nystatin sufficient to kill growing P. methanolica cells to select for cells having a deficiency in a nutritional gene; and (e) culturing the selected cells from step (d) in a rich culture medium.
PCT/US1997/012582 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants Ceased WO1998002536A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE69735369T DE69735369T2 (en) 1996-07-17 1997-07-14 PREPARATION OF AUXOTROPHIC MUTANES FROM PICHIA METHANOLICA
EP97936103A EP0946734B1 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
JP50632398A JP2002514049A (en) 1996-07-17 1997-07-14 Production of auxotrophic mutants of Pichia methanolica
CA002261020A CA2261020C (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
IL12807297A IL128072A0 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
AU38856/97A AU708572B2 (en) 1996-07-17 1997-07-14 Preparation of (pichia methanolica) auxotrophic mutants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US68350096A 1996-07-17 1996-07-17
US08/683,500 1996-07-17
US08/703,808 US5736383A (en) 1996-08-26 1996-08-26 Preparation of Pichia methanolica auxotrophic mutants
US08/703,808 1996-08-26

Publications (2)

Publication Number Publication Date
WO1998002536A2 WO1998002536A2 (en) 1998-01-22
WO1998002536A3 true WO1998002536A3 (en) 1998-02-26

Family

ID=27103122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/012582 Ceased WO1998002536A2 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants

Country Status (6)

Country Link
JP (1) JP2002514049A (en)
CN (1) CN1238806A (en)
AU (1) AU708572B2 (en)
CA (1) CA2261020C (en)
IL (1) IL128072A0 (en)
WO (1) WO1998002536A2 (en)

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WO2000017235A2 (en) 1998-09-23 2000-03-30 Zymogenetics, Inc. Cytokine receptor zalpha11
US7833529B1 (en) 1999-01-07 2010-11-16 Zymogenetics, Inc. Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor
PT1642972E (en) 1999-01-07 2010-04-07 Zymogenetics Inc Therapeutic uses of br43x2 soluble receptors
NZ514708A (en) 1999-03-09 2005-01-28 Zymogenetics Inc Novel cytokine zalpha11 ligand
WO2001004304A1 (en) 1999-07-07 2001-01-18 Zymogenetics, Inc. Human cytokine receptor
EP2194130A1 (en) 1999-12-23 2010-06-09 ZymoGenetics, L.L.C. Cytokine ZCYTO18
PT1325115T (en) 2000-06-26 2016-09-28 Zymogenetics Inc Cytokine receptor zcytor17
CA2438682A1 (en) 2001-02-20 2002-08-29 Zymogenetics, Inc. Antibodies that bind both bcma and taci
PT1436003E (en) 2001-05-24 2010-03-12 Zymogenetics Inc Taci-immunoglobulin fusion proteins
EP2305313B1 (en) 2001-10-10 2014-03-26 ratiopharm GmbH Remodelling and glycoconjugation of interferon-alpha (IFNa)
DK1451322T3 (en) 2001-11-05 2010-02-01 Zymogenetics Inc IL-21 antagonists
CN101843895A (en) 2002-01-18 2010-09-29 津莫吉尼蒂克斯公司 Cytokine receptor ZCYTOR17 multimers
AU2003280410B8 (en) 2002-01-18 2009-04-23 Zymogenetics, Inc. Cytokine receptor zcytor17 multimers
EP2295455A1 (en) 2002-04-19 2011-03-16 ZymoGenetics, L.L.C. Cytokine receptor
CA2522345A1 (en) 2003-04-09 2004-11-18 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
EP2163635B1 (en) 2004-08-02 2013-05-22 BASF Plant Science GmbH Method for isolation of transcription termination sequences
CN100347287C (en) * 2004-09-30 2007-11-07 汪和睦 Recombinated multi shape ttansenula yeast, its structural method and application
US20060177447A1 (en) 2005-02-08 2006-08-10 Wenfeng Xu Anti-IL-20, anti-IL-22 and anti-IL-22RA antibodies and binding partners and methods of using in inflammation
AU2006247591B2 (en) 2005-05-12 2011-12-15 Zymogenetics, Inc. Compositions and methods for modulating immune responses
UA93516C2 (en) 2005-08-09 2011-02-25 Займоджинетикс, Инк. Methods for treating b-cell malignancies using taci-ig fusion molecule
AU2006278227B2 (en) 2005-08-09 2011-10-20 Ares Trading S.A. Methods for the treatment and prevention of abnormal cell proliferation using TACI-fusion molecules
WO2007038703A2 (en) 2005-09-28 2007-04-05 Zymogenetics, Inc Il-17a and il-17f antagonists and methods of using the same
CA2651791A1 (en) 2006-05-15 2007-11-22 Ares-Trading S.A. Methods for treating autoimmune diseases using a taci-ig fusion molecule
AU2008328371B2 (en) 2007-11-21 2011-08-11 Roskilde Universitet Polypeptides comprising an ice-binding activity
AU2009207645B2 (en) 2008-01-25 2014-11-13 Aarhus Universitet Selective exosite inhibition of PAPP-A activity against IGFBP-4
EA201100943A1 (en) 2008-12-16 2012-05-30 Новартис Аг YEAST DISPLAY SYSTEMS
CA3156904A1 (en) 2012-02-01 2013-08-08 Synthetic Genomics, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides
CN111157680B (en) * 2019-12-31 2021-10-26 北京辰安科技股份有限公司 Indoor volatile substance leakage tracing method and device
CN116064597B (en) * 2023-01-17 2024-04-26 北京大学 Directed evolution and darwinian adaptation in mammalian cells by autonomous replication of RNA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica
WO1997017451A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BIOLOGICAL ABSTRACTS, vol. BA64, Philadelphia, PA, US; abstract no. 50226, TOLSTORUKOV I I ET AL: "HYBRIDIZATION AND GENETIC ANALYSIS OF THE METHANOL UTILIZING YEAST PICHIA-PINUS." XP002051202 *
BIOLOGICAL ABSTRACTS, vol. BA85, Philadelphia, PA, US; abstract no. 37426, TOLSTORUKOV I I ET AL: "GENETIC CONTROL OF PURINE BIOSYNTHESIS IN THE YEAST PICHIA PINUS I. GENUS CONTROLLING THE VI AND VII STEPS." XP002051203 *
GENETIKA 13 (2). 1977 322-329. CODEN: GNKAA5 ISSN: 0016-6758 *
GENETIKA 23 (8). 1987. 1399-1406. CODEN: GNKAA5 ISSN: 0016-6758 *
LAWRENCE C.W.: "Classical mutagenesis techniques", METHODS IN ENZYMOLOGY, vol. 194, 1991, pages 273 - 281, XP002050288 *
SNOW R.: "An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'", NATURE, vol. 211, 9 July 1966 (1966-07-09), pages 206 - 207, XP002050287 *
TOLSTORUKOV I I ET AL: "MUTANTS OF THE METHYLOTROPHIC YEAST PICHIA PINUS DEFECTIVE IN C-2 METABOLISM.", YEAST 5 (3). 1989. 179-186. CODEN: YESTE3 ISSN: 0749-503X, XP002051201 *

Also Published As

Publication number Publication date
WO1998002536A2 (en) 1998-01-22
CA2261020A1 (en) 1998-01-22
JP2002514049A (en) 2002-05-14
AU3885697A (en) 1998-02-09
AU708572B2 (en) 1999-08-05
IL128072A0 (en) 1999-11-30
CA2261020C (en) 2004-06-08
CN1238806A (en) 1999-12-15

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