CA2261020A1 - Preparation of pichia methanolica auxotrophic mutants - Google Patents
Preparation of pichia methanolica auxotrophic mutantsInfo
- Publication number
- CA2261020A1 CA2261020A1 CA002261020A CA2261020A CA2261020A1 CA 2261020 A1 CA2261020 A1 CA 2261020A1 CA 002261020 A CA002261020 A CA 002261020A CA 2261020 A CA2261020 A CA 2261020A CA 2261020 A1 CA2261020 A1 CA 2261020A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- culturing
- culture medium
- methanolica
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001452677 Ogataea methanolica Species 0.000 title abstract 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 6
- 238000012258 culturing Methods 0.000 abstract 4
- 239000001963 growth medium Substances 0.000 abstract 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 2
- 230000001413 cellular effect Effects 0.000 abstract 1
- 230000007812 deficiency Effects 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 229960000988 nystatin Drugs 0.000 abstract 1
- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000007320 rich medium Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Methods for preparing Pichia methanolica cells having auxotrophic mutations are disclosed. The methods comprise the steps of:
(a) exposing P. methanolica cells to mutagenizing conditions; (b) culturing the cells from step (a) in a rich medium to allow mutations to become established and replicated in at least a portion of the cells; (c) culturing the cells from step (b) in a culture medium deficient in assimilable nitrogen to deplete cellular nitrogen stores; (d) culturing the cells from step (c) in a defined culture medium comprising an inorganic nitrogen source and an amount of nystatin sufficient to kill growing P. methanolica cells to select for cells having a deficiency in a nutritional gene; and (e) culturing the selected cells from step (d) in a rich culture medium.
(a) exposing P. methanolica cells to mutagenizing conditions; (b) culturing the cells from step (a) in a rich medium to allow mutations to become established and replicated in at least a portion of the cells; (c) culturing the cells from step (b) in a culture medium deficient in assimilable nitrogen to deplete cellular nitrogen stores; (d) culturing the cells from step (c) in a defined culture medium comprising an inorganic nitrogen source and an amount of nystatin sufficient to kill growing P. methanolica cells to select for cells having a deficiency in a nutritional gene; and (e) culturing the selected cells from step (d) in a rich culture medium.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68350096A | 1996-07-17 | 1996-07-17 | |
| US60/042,910 | 1996-07-17 | ||
| US08/703,808 | 1996-08-26 | ||
| US08/703,808 US5736383A (en) | 1996-08-26 | 1996-08-26 | Preparation of Pichia methanolica auxotrophic mutants |
| PCT/US1997/012582 WO1998002536A2 (en) | 1996-07-17 | 1997-07-14 | Preparation of pichia methanolica auxotrophic mutants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2261020A1 true CA2261020A1 (en) | 1998-01-22 |
| CA2261020C CA2261020C (en) | 2004-06-08 |
Family
ID=27103122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002261020A Expired - Fee Related CA2261020C (en) | 1996-07-17 | 1997-07-14 | Preparation of pichia methanolica auxotrophic mutants |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JP2002514049A (en) |
| CN (1) | CN1238806A (en) |
| AU (1) | AU708572B2 (en) |
| CA (1) | CA2261020C (en) |
| IL (1) | IL128072A0 (en) |
| WO (1) | WO1998002536A2 (en) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000017235A2 (en) | 1998-09-23 | 2000-03-30 | Zymogenetics, Inc. | Cytokine receptor zalpha11 |
| PL209535B1 (en) | 1999-01-07 | 2011-09-30 | Zymogenetics Inc | SOLUBLE RECEPTOR BR43x2 AND METHODS OF USING THEM FOR THERAPY |
| US7833529B1 (en) | 1999-01-07 | 2010-11-16 | Zymogenetics, Inc. | Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor |
| IL145288A0 (en) | 1999-03-09 | 2002-06-30 | Zymogenetics Inc | Novel cytokin zalpha11 ligand |
| DK1190056T3 (en) | 1999-07-07 | 2007-07-30 | Zymogenetics Inc | Human cytokine receptor |
| AU2292601A (en) | 1999-12-23 | 2001-07-03 | Zymogenetics Inc. | Novel cytokine zcyto18 |
| AU2001273032A1 (en) | 2000-06-26 | 2002-01-08 | Zymogenetics Inc. | Cytokine receptor zcytor17 |
| AU2002238052A1 (en) | 2001-02-20 | 2002-09-04 | Zymogenetics, Inc. | Antibodies that bind both bcma and taci |
| DE60234202D1 (en) | 2001-05-24 | 2009-12-10 | Zymogenetics Inc | TACI-IMMUNOGLOBULIN FUSION PROTEINS |
| ES2411007T3 (en) | 2001-10-10 | 2013-07-04 | Novo Nordisk A/S | Remodeling and glycoconjugation of peptides |
| EP1451322B1 (en) | 2001-11-05 | 2009-09-30 | ZymoGenetics, Inc. | Il-21 antagonists |
| EP2338910B1 (en) | 2002-01-18 | 2015-07-08 | ZymoGenetics, Inc. | Cytokine receptor Zcytor17 multimers |
| DK1476541T3 (en) | 2002-01-18 | 2008-11-03 | Zymogenetics Inc | Cytokine (zcytor17 ligand) |
| EP1497415B1 (en) | 2002-04-19 | 2010-12-29 | ZymoGenetics, L.L.C. | Methods for detection or modulation of the interaction of a cytokine receptor with its ligand |
| PL1615945T3 (en) | 2003-04-09 | 2012-03-30 | Ratiopharm Gmbh | Glycopegylation methods and proteins/peptides produced by the methods |
| CA2834275A1 (en) | 2004-08-02 | 2006-02-09 | Basf Plant Science Gmbh | Method for isolation of transcription termination sequences |
| CN100347287C (en) * | 2004-09-30 | 2007-11-07 | 汪和睦 | Recombinated multi shape ttansenula yeast, its structural method and application |
| AU2006212807A1 (en) | 2005-02-08 | 2006-08-17 | Zymogenetics, Inc. | Anti-IL-20, anti-IL-22 and anti-IL-22RA antibodies and binding partners and methods of using in inflammation |
| EP3214095B1 (en) | 2005-05-12 | 2019-12-11 | ZymoGenetics, Inc. | Compositions and methods for modulating immune responses |
| MX2008001661A (en) | 2005-08-09 | 2008-04-07 | Ares Trading Sa | Methods for treating b-cell malignancies using taci-ig fusion molecule. |
| AU2006278227B2 (en) | 2005-08-09 | 2011-10-20 | Ares Trading S.A. | Methods for the treatment and prevention of abnormal cell proliferation using TACI-fusion molecules |
| WO2007038703A2 (en) | 2005-09-28 | 2007-04-05 | Zymogenetics, Inc | Il-17a and il-17f antagonists and methods of using the same |
| US8784812B2 (en) | 2006-05-15 | 2014-07-22 | Zymogenetics, Inc. | Methods for treating autoimmune diseases using a TACI-Ig fusion molecule |
| DK2602263T3 (en) | 2007-11-21 | 2019-10-21 | Roskilde Univ | Polypeptides comprising an ice-binding activity |
| US8653020B2 (en) | 2008-01-25 | 2014-02-18 | Aarhus Universitet | Selective exosite inhibition of PAPP-A activity against IGFBP-4 |
| CA2746840A1 (en) | 2008-12-16 | 2010-06-24 | Novartis Ag | Yeast display systems |
| US9771576B2 (en) | 2012-02-01 | 2017-09-26 | Synthetic Genomics, Inc. | Materials and methods for the synthesis of error-minimized nucleic acid molecules |
| WO2014202089A2 (en) | 2013-06-18 | 2014-12-24 | Roskilde Universitet | Variants of anti-freeze polypeptides |
| CN111157680B (en) * | 2019-12-31 | 2021-10-26 | 北京辰安科技股份有限公司 | Indoor volatile substance leakage tracing method and device |
| CN116064597B (en) * | 2023-01-17 | 2024-04-26 | 北京大学 | Directed evolution and darwinian adaptation in mammalian cells by autonomous replication of RNA |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2237039A1 (en) * | 1995-11-09 | 1997-05-15 | Zymogenetics, Inc. | Production of gad65 in methylotrophic yeast |
-
1997
- 1997-07-14 IL IL12807297A patent/IL128072A0/en unknown
- 1997-07-14 AU AU38856/97A patent/AU708572B2/en not_active Ceased
- 1997-07-14 JP JP50632398A patent/JP2002514049A/en not_active Ceased
- 1997-07-14 CA CA002261020A patent/CA2261020C/en not_active Expired - Fee Related
- 1997-07-14 WO PCT/US1997/012582 patent/WO1998002536A2/en not_active Ceased
- 1997-07-14 CN CN97197503.5A patent/CN1238806A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002514049A (en) | 2002-05-14 |
| IL128072A0 (en) | 1999-11-30 |
| CN1238806A (en) | 1999-12-15 |
| CA2261020C (en) | 2004-06-08 |
| AU708572B2 (en) | 1999-08-05 |
| AU3885697A (en) | 1998-02-09 |
| WO1998002536A2 (en) | 1998-01-22 |
| WO1998002536A3 (en) | 1998-02-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |