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WO1998051785A1 - Co-culture de cellules dans une configuration micromodelisee - Google Patents

Co-culture de cellules dans une configuration micromodelisee Download PDF

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Publication number
WO1998051785A1
WO1998051785A1 PCT/US1997/008254 US9708254W WO9851785A1 WO 1998051785 A1 WO1998051785 A1 WO 1998051785A1 US 9708254 W US9708254 W US 9708254W WO 9851785 A1 WO9851785 A1 WO 9851785A1
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WO
WIPO (PCT)
Prior art keywords
cells
cell
cell type
culture
hepatocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/008254
Other languages
English (en)
Inventor
Sangeeta Bhatia
Martin Yarmush
Mehmet Toner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Massachusetts Institute of Technology
Original Assignee
General Hospital Corp
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp, Massachusetts Institute of Technology filed Critical General Hospital Corp
Priority to JP54917598A priority Critical patent/JP2002510969A/ja
Priority to PCT/US1997/008254 priority patent/WO1998051785A1/fr
Publication of WO1998051785A1 publication Critical patent/WO1998051785A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • External Artificial Organs (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés de production de co-cultures de cellules dont au moins deux types sont configurés dans un micromodèle sur un substrat de façon que la modulation de l'étendue des contacts cellules-cellules hétérotypiques puisse être utilisée pour moduler des fonctions métaboliques et/ou synthétiques des cellules. On peut mettre en oeuvre la co-culture en revêtant une protéine telle que le collagène sur un substrat de façon que la protéine définisse un micromodèle sur le substrat, en mettant en contact le substrat revêtu avec des cellules d'un premier type telles que les hépatocytes dans un milieu afin de lier les cellules avec la protéine dans le but de produire un substrat revêtu de cellules micromodélisées et en mettant en contact le substrat micromodélisé avec les cellules d'un second type telles que les fibroblastes dans un second milieu afin de lier les cellules au substrat. Les deux milieux sont pour l'un, un milieu de non fixation, tel qu'un milieu exempt de sérum, et pour l'autre, un milieu de fixation tel qu'un milieu renfermant du sérum. Le milieu de fixation peut contenir un facteur de fixation tel que la fibronectine ou un anticorps. Le micromodèle peut être formé d'îlots de cellules du premier ou second type entourés de cellules de l'autre type.
PCT/US1997/008254 1997-05-14 1997-05-14 Co-culture de cellules dans une configuration micromodelisee Ceased WO1998051785A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP54917598A JP2002510969A (ja) 1997-05-14 1997-05-14 マイクロパターン化形態における細胞の共培養
PCT/US1997/008254 WO1998051785A1 (fr) 1997-05-14 1997-05-14 Co-culture de cellules dans une configuration micromodelisee

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1997/008254 WO1998051785A1 (fr) 1997-05-14 1997-05-14 Co-culture de cellules dans une configuration micromodelisee

Publications (1)

Publication Number Publication Date
WO1998051785A1 true WO1998051785A1 (fr) 1998-11-19

Family

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Family Applications (1)

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PCT/US1997/008254 Ceased WO1998051785A1 (fr) 1997-05-14 1997-05-14 Co-culture de cellules dans une configuration micromodelisee

Country Status (2)

Country Link
JP (1) JP2002510969A (fr)
WO (1) WO1998051785A1 (fr)

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1428871A4 (fr) * 2001-07-26 2004-11-24 Kazunori Kataoka Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante
JP2007244875A (ja) * 1999-02-01 2007-09-27 Orthogen Ag 体液から治療上有効なタンパク質であるインターロイキン−１受容体拮抗薬を産生するための方法
EP1904625A4 (fr) * 2005-05-24 2009-12-23 Univ California Tissus in vitro élaborés, microstructurés et à micro-échelle
US7687251B2 (en) 2004-03-26 2010-03-30 Dai Nippon Printing Co., Ltd. Method for producing cell culture substrate and apparatus for producing cell culture substrate
US7919305B2 (en) 2004-02-19 2011-04-05 Dai Nippon Printing Co., Ltd. Method for manufacturing cell culture substrate
WO2012027218A2 (fr) 2010-08-27 2012-03-01 Corning Incorporated Surfaces modifiées par un peptide pour culture cellulaire
AU2012216645B2 (en) * 2005-05-24 2015-06-25 The Regents Of The University Of California Microscale micropatterned engineered in vitro tissue
US9249386B2 (en) 2005-06-06 2016-02-02 Dai Nippon Printing Co., Ltd. Substrate for cell transfer
US10266806B2 (en) * 2014-01-14 2019-04-23 Colorado State University Research Foundation Stem cell-derived hepatocytes in co-culture and uses thereof
US11407979B2 (en) 2017-09-01 2022-08-09 Colorado State University Research Foundation Intermittent starvation techniques to prolong functional lifetime of liver cells in culture
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US12043823B2 (en) 2021-03-23 2024-07-23 Terumo Bct, Inc. Cell capture and expansion
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
US12180507B2 (en) 2015-08-05 2024-12-31 Colorado State University Research Foundation Engineered platforms to stabilize both hepatocytes and endothelial cells in vitro
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6943021B2 (en) * 2002-06-07 2005-09-13 Mattek Corporation Three dimensional vaginal tissue model containing immune cells
JP4565916B2 (ja) * 2004-07-23 2010-10-20 三洋電機株式会社 光反応装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4614517A (en) * 1982-08-04 1986-09-30 La Jolla Cancer Research Foundation Tetrapeptide
US5602029A (en) * 1994-06-27 1997-02-11 Nec Corporation Method for fabricating substrate for cell culture and method for cell arrangements

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4614517A (en) * 1982-08-04 1986-09-30 La Jolla Cancer Research Foundation Tetrapeptide
US5602029A (en) * 1994-06-27 1997-02-11 Nec Corporation Method for fabricating substrate for cell culture and method for cell arrangements

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BHATIA et al., "Controlling Cell Interactions by Micropatterning in Co-Cultures: Hepatocytes and 3T3 Fibroblasts", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, February 1997, Vol. 34, No. 2, pages 189-199. *
IRELAND et al., "Effect of Patterned Surfaces of Adhesive Islands on the Shape, Cytoskeleton, Adhesion and Behaviour of Swiss Mouse 3T3 Fibroblasts", JOURNAL OF CELL SCIENCE SUPPLEMENT, 1987, Vol. 8, pages 19-33. *

Cited By (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007244875A (ja) * 1999-02-01 2007-09-27 Orthogen Ag 体液から治療上有効なタンパク質であるインターロイキン−１受容体拮抗薬を産生するための方法
EP1428871A4 (fr) * 2001-07-26 2004-11-24 Kazunori Kataoka Construction cellulaire cultivee contenant des spheroides de cellules animales cultivees et utilisation correspondante
US7919305B2 (en) 2004-02-19 2011-04-05 Dai Nippon Printing Co., Ltd. Method for manufacturing cell culture substrate
US8497117B2 (en) 2004-02-19 2013-07-30 Dai Nippon Printing Co., Ltd. Method for manufacturing cell culture substrate
US7687251B2 (en) 2004-03-26 2010-03-30 Dai Nippon Printing Co., Ltd. Method for producing cell culture substrate and apparatus for producing cell culture substrate
EP1904625A4 (fr) * 2005-05-24 2009-12-23 Univ California Tissus in vitro élaborés, microstructurés et à micro-échelle
AU2006250079B2 (en) * 2005-05-24 2012-05-31 The Regents Of The University Of California Microscale micropatterned engineered in vitor tissue
EP2495310A3 (fr) * 2005-05-24 2013-03-27 The Regents Of the University of California Microfiltration à micromotifs créée dans des tissus in vitro
AU2012216645B2 (en) * 2005-05-24 2015-06-25 The Regents Of The University Of California Microscale micropatterned engineered in vitro tissue
US9249386B2 (en) 2005-06-06 2016-02-02 Dai Nippon Printing Co., Ltd. Substrate for cell transfer
WO2012027218A2 (fr) 2010-08-27 2012-03-01 Corning Incorporated Surfaces modifiées par un peptide pour culture cellulaire
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11773363B2 (en) 2010-10-08 2023-10-03 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11746319B2 (en) 2010-10-08 2023-09-05 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11708554B2 (en) 2013-11-16 2023-07-25 Terumo Bct, Inc. Expanding cells in a bioreactor
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US10266806B2 (en) * 2014-01-14 2019-04-23 Colorado State University Research Foundation Stem cell-derived hepatocytes in co-culture and uses thereof
US10717966B2 (en) 2014-01-14 2020-07-21 Colorado State University Research Foundation Stem cell-derived hepatocytes in co-culture and uses thereof
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US12065637B2 (en) 2014-09-26 2024-08-20 Terumo Bct, Inc. Scheduled feed
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US12180507B2 (en) 2015-08-05 2024-12-31 Colorado State University Research Foundation Engineered platforms to stabilize both hepatocytes and endothelial cells in vitro
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11999929B2 (en) 2016-06-07 2024-06-04 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US12077739B2 (en) 2016-06-07 2024-09-03 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11702634B2 (en) 2017-03-31 2023-07-18 Terumo Bct, Inc. Expanding cells in a bioreactor
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
US12359170B2 (en) 2017-03-31 2025-07-15 Terumo Bct, Inc. Expanding cells in a bioreactor
US11407979B2 (en) 2017-09-01 2022-08-09 Colorado State University Research Foundation Intermittent starvation techniques to prolong functional lifetime of liver cells in culture
US12043823B2 (en) 2021-03-23 2024-07-23 Terumo Bct, Inc. Cell capture and expansion
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
US12209689B2 (en) 2022-02-28 2025-01-28 Terumo Kabushiki Kaisha Multiple-tube pinch valve assembly
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device

Also Published As

Publication number Publication date
JP2002510969A (ja) 2002-04-09

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