WO1997014815B1 - Procedes pour preparer des supports solides destines a l'hybridation et pour reduire un fond non specifique - Google Patents
Procedes pour preparer des supports solides destines a l'hybridation et pour reduire un fond non specifiqueInfo
- Publication number
- WO1997014815B1 WO1997014815B1 PCT/US1996/016777 US9616777W WO9714815B1 WO 1997014815 B1 WO1997014815 B1 WO 1997014815B1 US 9616777 W US9616777 W US 9616777W WO 9714815 B1 WO9714815 B1 WO 9714815B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hybridization
- solid supports
- solid support
- specific background
- methods
- Prior art date
Links
- 239000007787 solid Substances 0.000 title claims abstract 10
- 238000009396 hybridization Methods 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims abstract 5
- 239000003446 ligand Substances 0.000 claims abstract 5
- 150000001875 compounds Chemical class 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000009871 nonspecific binding Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
Abstract
L'invention concerne des procédés relatifs aux supports solides destinés à l'hybridation. Elle décrit des techniques de préparation des supports solides, ainsi que leur utilisation dans des analyses d'hybridation, de façon à réduire un fond non spécifique couvrant les supports solides. La réduction du fond non spécifique permet de détecter des taux faibles de fixations spécifiques, qui normalement seraient masquées par les fixations non spécifiques. Ces procédés peuvent être appliqués à divers ligands cibles et sondes, entre autres aux acides nucléiques tels que les oligonucléotides.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980708439A KR19990064358A (ko) | 1995-10-18 | 1996-10-17 | 하이브리드화용 고체 지지체의 제조 방법 및 비-특이적 배경의 감소 방법 |
| EP96937697A EP0862655B1 (fr) | 1995-10-18 | 1996-10-17 | Procedes pour preparer des supports solides destines a l'hybridation et pour reduire un fond non specifique |
| CA002235671A CA2235671A1 (fr) | 1995-10-18 | 1996-10-17 | Procedes pour preparer des supports solides destines a l'hybridation et pour reduire un fond non specifique |
| AT96937697T ATE230032T1 (de) | 1995-10-18 | 1996-10-17 | Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund |
| AU75174/96A AU723889B2 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
| DE69625510T DE69625510T2 (de) | 1995-10-18 | 1996-10-17 | Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund |
| JP9516062A JPH11514872A (ja) | 1995-10-18 | 1996-10-17 | ハイブリダイゼーション用の固体支持体を調製する方法および非特異的バックグラウンドを低減する方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US650195P | 1995-10-18 | 1995-10-18 | |
| US60/006,501 | 1995-10-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997014815A1 WO1997014815A1 (fr) | 1997-04-24 |
| WO1997014815B1 true WO1997014815B1 (fr) | 1997-06-05 |
Family
ID=21721191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/016777 WO1997014815A1 (fr) | 1995-10-18 | 1996-10-17 | Procedes pour preparer des supports solides destines a l'hybridation et pour reduire un fond non specifique |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5994065A (fr) |
| EP (1) | EP0862655B1 (fr) |
| JP (1) | JPH11514872A (fr) |
| KR (1) | KR19990064358A (fr) |
| AT (1) | ATE230032T1 (fr) |
| AU (1) | AU723889B2 (fr) |
| CA (1) | CA2235671A1 (fr) |
| DE (1) | DE69625510T2 (fr) |
| WO (1) | WO1997014815A1 (fr) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2274587A1 (fr) | 1996-12-10 | 1998-06-18 | Genetrace Systems Inc. | Molecules d'etiquetage massique, non volatiles et liberables |
| US7173011B2 (en) | 2000-11-20 | 2007-02-06 | University Of Southern California | Radiation therapy methods |
| WO1999054509A1 (fr) * | 1998-04-20 | 1999-10-28 | Affymetrix, Inc. | Reduction des liaisons non specifiques avec une matrice de sondes d'acides nucleiques |
| JP2002528093A (ja) * | 1998-10-23 | 2002-09-03 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 表面における核酸の単離及び精製のための方法並びに手段 |
| AU6052001A (en) * | 2000-03-29 | 2001-10-08 | Ct For The Applic Of Molecular | Methods for genotyping by hybridization analysis |
| DE10027120A1 (de) * | 2000-05-23 | 2001-12-06 | Epigenomics Ag | Probenträger für Massenspektrometer |
| WO2002040962A2 (fr) * | 2000-11-15 | 2002-05-23 | Benchmark Science, Inc. | Procedes et reactifs permettant de reduire les activites de fond de dosages biologiques heterogenes |
| JP3523188B2 (ja) * | 2000-12-13 | 2004-04-26 | 富士写真フイルム株式会社 | プローブ分子が固定された検出具の水性処理液 |
| DE10130800B4 (de) | 2001-06-22 | 2005-06-23 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität |
| JP2003084002A (ja) | 2001-06-27 | 2003-03-19 | Fuji Photo Film Co Ltd | 蛍光検出におけるバックグラウンドの低減方法 |
| US20030082969A1 (en) * | 2001-10-25 | 2003-05-01 | Trevor Arthurs | Breathable barrier extruded laminate |
| DE10154317B4 (de) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| GB2392977A (en) * | 2002-09-13 | 2004-03-17 | Suisse Electronique Microtech | A fluidic dielectrophoretic system and method for analysing biomolecules |
| AU2002952702A0 (en) * | 2002-11-18 | 2002-11-28 | Murdoch Childrens Research Institute | A diagnostic assay |
| US7368082B1 (en) * | 2002-12-12 | 2008-05-06 | Tung-Lian Huang | Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles |
| AU2006244394B2 (en) | 2005-05-06 | 2010-10-21 | Gen-Probe Incorporated | Methods of nucleic acid target capture |
| US20060257958A1 (en) * | 2005-05-13 | 2006-11-16 | Pronucleotein Biotechnologies, Llc | Magnetically-assisted test strip cartridge and method for using same |
| WO2009137137A2 (fr) * | 2008-02-19 | 2009-11-12 | Intelligentmdx, Inc. | Sondes et amorces optimisées et procédés pour les utiliser pour la détection et la quantification de virus bk |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| WO2009126517A2 (fr) * | 2008-04-09 | 2009-10-15 | Intelligent Medical Devices, Inc. | Sondes et amorces optimisées, et procédés d’utilisation de celles-ci dans la détection, la quantification et le typage du vih-1 |
| US20110200995A1 (en) | 2009-09-04 | 2011-08-18 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci |
| US8877909B2 (en) | 2011-04-04 | 2014-11-04 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus |
| US20140127674A1 (en) | 2012-10-25 | 2014-05-08 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the binding, detection, differentiation, isolation and sequencing of influenza a; influenza b and respiratory syncytial virus |
| US20140256582A1 (en) | 2013-03-05 | 2014-09-11 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
| DE69130396T2 (de) * | 1990-08-16 | 1999-06-24 | Genelabs Diagnostics Pte Ltd., Singapore | Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern |
| WO1994000600A1 (fr) * | 1992-06-25 | 1994-01-06 | Microprobe Corporation | Supports solides pour dosages d'hybridation de l'acide nucleique |
-
1996
- 1996-10-17 AU AU75174/96A patent/AU723889B2/en not_active Ceased
- 1996-10-17 US US08/733,671 patent/US5994065A/en not_active Expired - Lifetime
- 1996-10-17 DE DE69625510T patent/DE69625510T2/de not_active Expired - Fee Related
- 1996-10-17 AT AT96937697T patent/ATE230032T1/de not_active IP Right Cessation
- 1996-10-17 EP EP96937697A patent/EP0862655B1/fr not_active Expired - Lifetime
- 1996-10-17 KR KR1019980708439A patent/KR19990064358A/ko not_active Withdrawn
- 1996-10-17 CA CA002235671A patent/CA2235671A1/fr not_active Abandoned
- 1996-10-17 JP JP9516062A patent/JPH11514872A/ja active Pending
- 1996-10-17 WO PCT/US1996/016777 patent/WO1997014815A1/fr active IP Right Grant
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