WO1997014815B1 - Methods for preparing solid supports for hybridization and reducing non-specific background - Google Patents
Methods for preparing solid supports for hybridization and reducing non-specific backgroundInfo
- Publication number
- WO1997014815B1 WO1997014815B1 PCT/US1996/016777 US9616777W WO9714815B1 WO 1997014815 B1 WO1997014815 B1 WO 1997014815B1 US 9616777 W US9616777 W US 9616777W WO 9714815 B1 WO9714815 B1 WO 9714815B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hybridization
- solid supports
- solid support
- specific background
- methods
- Prior art date
Links
- 239000007787 solid Substances 0.000 title claims abstract 10
- 238000009396 hybridization Methods 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims abstract 5
- 239000003446 ligand Substances 0.000 claims abstract 5
- 150000001875 compounds Chemical class 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000009871 nonspecific binding Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
Abstract
Method related to solid supports for hybridization are disclosed. The present invention provides procedures for preparing solid supports, and their use in hybridization assays, such that non-specific background on the solid supports is reduced. The reduction of non-specific background permits the detection of low levels of specific binding which normally would be masked by the non-specific binding. The methods are applicable to a variety of target ligands and probes, including nucleic acids such as oligonucleotides.
Claims
1. A method for preparing hybridization solid supports that reduces non¬ specific background, comprising the steps of:
(a) contacting a solid support for hybridization with a target ligand under conditions sufficient to immobilize said target ligand to said solid support; and
(b) reacting said solid support containing immobilized target ligand with a compound under conditions sufficient to block non-specific sites on said solid support, thereby producing a blocked solid support containing immobilized target ligand, said compound having the formula:
(I) (II)
(IH) (IV)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980708439A KR19990064358A (en) | 1995-10-18 | 1996-10-17 | Process for preparing solid support for hybridization and method for reducing non-specific background |
| EP96937697A EP0862655B1 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
| CA002235671A CA2235671A1 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
| AT96937697T ATE230032T1 (en) | 1995-10-18 | 1996-10-17 | METHOD FOR PRODUCING SOLID SUPPORTS FOR HYBRIDIZATION AND NON-SPECIFIC BACKGROUND REDUCTION |
| AU75174/96A AU723889B2 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
| DE69625510T DE69625510T2 (en) | 1995-10-18 | 1996-10-17 | METHOD FOR PRODUCING SOLID CARRIERS FOR HYBRIDIZING AND REDUCING NON-SPECIFIC BACKGROUND |
| JP9516062A JPH11514872A (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and methods for reducing non-specific background |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US650195P | 1995-10-18 | 1995-10-18 | |
| US60/006,501 | 1995-10-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997014815A1 WO1997014815A1 (en) | 1997-04-24 |
| WO1997014815B1 true WO1997014815B1 (en) | 1997-06-05 |
Family
ID=21721191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/016777 WO1997014815A1 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5994065A (en) |
| EP (1) | EP0862655B1 (en) |
| JP (1) | JPH11514872A (en) |
| KR (1) | KR19990064358A (en) |
| AT (1) | ATE230032T1 (en) |
| AU (1) | AU723889B2 (en) |
| CA (1) | CA2235671A1 (en) |
| DE (1) | DE69625510T2 (en) |
| WO (1) | WO1997014815A1 (en) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2274587A1 (en) | 1996-12-10 | 1998-06-18 | Genetrace Systems Inc. | Releasable nonvolatile mass-label molecules |
| US7173011B2 (en) | 2000-11-20 | 2007-02-06 | University Of Southern California | Radiation therapy methods |
| WO1999054509A1 (en) * | 1998-04-20 | 1999-10-28 | Affymetrix, Inc. | Methods for reducing non-specific binding to a nucleic acid probe array |
| JP2002528093A (en) * | 1998-10-23 | 2002-09-03 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | Methods and means for isolation and purification of nucleic acids on surfaces |
| AU6052001A (en) * | 2000-03-29 | 2001-10-08 | Ct For The Applic Of Molecular | Methods for genotyping by hybridization analysis |
| DE10027120A1 (en) * | 2000-05-23 | 2001-12-06 | Epigenomics Ag | Sample holder for mass spectrometer |
| WO2002040962A2 (en) * | 2000-11-15 | 2002-05-23 | Benchmark Science, Inc. | Methods and reagents to reduce backgrounds of heterogeneous assays |
| JP3523188B2 (en) * | 2000-12-13 | 2004-04-26 | 富士写真フイルム株式会社 | Aqueous treatment solution for detector with immobilized probe molecules |
| DE10130800B4 (en) | 2001-06-22 | 2005-06-23 | Epigenomics Ag | Method for the detection of cytosine methylation with high sensitivity |
| JP2003084002A (en) | 2001-06-27 | 2003-03-19 | Fuji Photo Film Co Ltd | Method for reducing background in fluorescence detection |
| US20030082969A1 (en) * | 2001-10-25 | 2003-05-01 | Trevor Arthurs | Breathable barrier extruded laminate |
| DE10154317B4 (en) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Method for the detection of cytosine methylations in immobilized DNA samples |
| GB2392977A (en) * | 2002-09-13 | 2004-03-17 | Suisse Electronique Microtech | A fluidic dielectrophoretic system and method for analysing biomolecules |
| AU2002952702A0 (en) * | 2002-11-18 | 2002-11-28 | Murdoch Childrens Research Institute | A diagnostic assay |
| US7368082B1 (en) * | 2002-12-12 | 2008-05-06 | Tung-Lian Huang | Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles |
| AU2006244394B2 (en) | 2005-05-06 | 2010-10-21 | Gen-Probe Incorporated | Methods of nucleic acid target capture |
| US20060257958A1 (en) * | 2005-05-13 | 2006-11-16 | Pronucleotein Biotechnologies, Llc | Magnetically-assisted test strip cartridge and method for using same |
| WO2009137137A2 (en) * | 2008-02-19 | 2009-11-12 | Intelligentmdx, Inc. | Optimized probes and primers and methods of using same for the detection and quantitation of bk virus |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| WO2009126517A2 (en) * | 2008-04-09 | 2009-10-15 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, quantification and grouping of hiv-1 |
| US20110200995A1 (en) | 2009-09-04 | 2011-08-18 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci |
| US8877909B2 (en) | 2011-04-04 | 2014-11-04 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus |
| US20140127674A1 (en) | 2012-10-25 | 2014-05-08 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the binding, detection, differentiation, isolation and sequencing of influenza a; influenza b and respiratory syncytial virus |
| US20140256582A1 (en) | 2013-03-05 | 2014-09-11 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
| DE69130396T2 (en) * | 1990-08-16 | 1999-06-24 | Genelabs Diagnostics Pte Ltd., Singapore | ENLARGED WESTERN BLOT FORMAT AND AN IMMUNOTESTING PROCEDURE FOR DETERMINING VIRAL ANTIBODIES |
| WO1994000600A1 (en) * | 1992-06-25 | 1994-01-06 | Microprobe Corporation | Solid supports for nucleic acid hybridization assays |
-
1996
- 1996-10-17 AU AU75174/96A patent/AU723889B2/en not_active Ceased
- 1996-10-17 US US08/733,671 patent/US5994065A/en not_active Expired - Lifetime
- 1996-10-17 DE DE69625510T patent/DE69625510T2/en not_active Expired - Fee Related
- 1996-10-17 AT AT96937697T patent/ATE230032T1/en not_active IP Right Cessation
- 1996-10-17 EP EP96937697A patent/EP0862655B1/en not_active Expired - Lifetime
- 1996-10-17 KR KR1019980708439A patent/KR19990064358A/en not_active Withdrawn
- 1996-10-17 CA CA002235671A patent/CA2235671A1/en not_active Abandoned
- 1996-10-17 JP JP9516062A patent/JPH11514872A/en active Pending
- 1996-10-17 WO PCT/US1996/016777 patent/WO1997014815A1/en active IP Right Grant
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