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WO1997008327A1 - Gene de l'acetolactate synthase resistant aux herbicides - Google Patents

Gene de l'acetolactate synthase resistant aux herbicides Download PDF

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Publication number
WO1997008327A1
WO1997008327A1 PCT/JP1996/002448 JP9602448W WO9708327A1 WO 1997008327 A1 WO1997008327 A1 WO 1997008327A1 JP 9602448 W JP9602448 W JP 9602448W WO 9708327 A1 WO9708327 A1 WO 9708327A1
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Prior art keywords
nucleic acid
acid fragment
herbicide
amino acid
plant
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PCT/JP1996/002448
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English (en)
Japanese (ja)
Inventor
Takaomi Fushimi
Kunimitsu Nakahira
Michito Tagawa
Tsutomu Nawamaki
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Nissan Chemical Corp
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Nissan Chemical Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Definitions

  • the present invention relates to a mutated nucleic acid fragment exhibiting resistance to a herbicide such as sulfonylprea that inhibits plant acetolactate synthase, by introducing the above-mentioned gene into a herbicide-sensitive plant.
  • the present invention relates to a nucleic acid fragment intended to impart resistance to a herbicide and to be used as a selection marker when introducing a gene into a plant.
  • weed control mainly involves chemical agents, but selective herbicides that do not cause harm to target crops are used for such chemical agents.
  • selective herbicides that do not cause harm to target crops are used for such chemical agents.
  • a large number of herbicide-resistant genes that exhibit resistance to non-selective herbicides have been isolated by genetic engineering techniques. The plants shown can be produced.
  • a promoter and an enhancer are used upstream of the gene of interest, and a recombinant DNA with a transcription termination signal added downstream is generally used.
  • a method using a patterm is used, but a particle gun method, an electroporation method, a polyethylene glycol method, and the like are also used.
  • a gene showing resistance to phosphinothricin, a non-selective herbicide is introduced into a plant such as tobacco using agrobacterium, and expressed in plant cells.
  • phosphinothricin a non-selective herbicide
  • a full-length gene has been isolated from a puffer tree as one of the resistance genes to sulfonylurea herbicides, and by analyzing its structure, mutations in amino acids involved in resistance have been elucidated. If a gene can be introduced into a plant, the resistance to herbicides can be easily imparted to the plant, and it should be used as a selection tool when introducing the gene into the plant. Becomes possible.
  • the present inventors focused on the above-mentioned report that pufferfish is resistant to a sulfonylurea herbicide, and used the known gene sequence of the main A region of the acetolacto-synthetase gene to develop resistance to the herbicide.
  • the full-length gene was isolated from the cDNA of Butterfly, which showed the following expression.
  • the new amino acid mutation can be made resistant to The present invention.
  • the present invention relates to a nucleic acid fragment encoding plant acetolactate synthase, wherein the amino acid sequence translated from the nucleotide sequence of the nucleic acid fragment includes ⁇ in SEQ ID NO: 1 in the sequence listing. , ⁇ , 7, 5 comprising at least one nucleotide fragment encoding one amino acid of the substantially conserved amino acid sequence, wherein said nucleotide fragment is
  • a has a sequence encoding an amino acid other than aspartic acid and glutamic acid;
  • b) ⁇ has a sequence encoding an amino acid other than valine
  • c) has a sequence encoding an amino acid other than tryptophan
  • d) ⁇ has a sequence encoding an amino acid other than asparagine
  • nucleic acid fragment satisfies at least one condition of The above ⁇ is alanine, arginine, asparagine, cystine, gel Cow
  • glycine histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine are preferable, and glycine is preferable.
  • the above include: alanine, arginine, asparagine, aspartic acid, cystine, glutamic acid, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threbonin And tyrosine, and preferably glumic acid.
  • the above 7 includes: alanine, arginine, asparagine, aspartic acid, cystine, glutamic acid, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine. , Tyrosine and balin, and preferably arginine.
  • the above S includes alanine, arginine, aspartic acid, cisdin, dalsiminic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tributanphan , Tyrosine and palin, and preferably serine.
  • glycine is glutamic acid
  • y is arginine
  • ⁇ is serine
  • the present invention also relates to a protein translated from the nucleic acid fragment, which is characterized by having resistance to a sulfonylurea herbicide.
  • the present invention relates to a method for imparting resistance to a sulfonylurea herbicide to a plant by expressing all or part of the gene in a plant sensitive to the sulfonylurea herbicide. Related.
  • a method for selecting a plant cell transformed with the nucleic acid fragment comprising transgene-introducing all or a part of the nucleic acid fragment into a plant cell sensitive to a sulfonylprea herbicide, And selecting transformed cells with a selective concentration of the herbicide that inhibits the growth of untransformed cells.
  • the nucleic acid fragment encoding acetacetate synthase conferring herbicide resistance is a nucleic acid fragment bound to the second nucleic acid fragment conferring the second property, and is used for transforming a plant.
  • the present invention also relates to the binding nucleic acid fragment, wherein the expression of herbicide resistance by the plant is used to detect the presence of the second nucleic acid fragment when a sulfonylprea herbicide is applied. .
  • Figure 1 shows the deduced amino acid sequence of the sulfonylurea herbicide-resistant asteracetate synthase shown in SEQ ID NO: 1 in the sequence listing, which was previously reported from an acetate from a sulfonylurea-sensitive plant. The result compared with the amino acid sequence of lactate synthase is shown.
  • A shows the amino acid sequence of a sulfonylurea herbicide-resistant peas perilla.
  • a and A indicate the amino acid sequences of the radish
  • B the tobacco
  • C the Arabidopsis
  • D rapeseed
  • E the corn.
  • An asterisk (*) is shown below the conserved part in each amino acid sequence. Amino acids are indicated by the following one-letter codes.
  • FIG. 2 shows a continuation of FIG.
  • FIG. 3 shows a continuation of FIG.
  • FIG. 4 shows a continuation of FIG.
  • 5 to 7 are schemes showing a method for constructing an expression vector in the present invention. Detailed description of the invention
  • the DNA sequence encoding the acetate reductase synthase exhibiting sulfonylprea herbicide resistance of the present invention specifically includes a single open reading frame as shown in SEQ ID NO: 1 in the sequence listing. It consists of going out.
  • the open reading frame has a translation start at the 5 'end from ATG having a base sequence of 54 to 56, an untranslated region of 53 bases upstream of the ATG, and a TGA having a base sequence of 2052 to 2054 at the 3' end.
  • the sequence is completed, and the sequences AA AT AA and TA AT AA, which are presumed to be poly (A) -added signals, are present downstream of the sequence.
  • the nucleotide sequence of this cDNA includes the nucleotide sequence of the domain A region that has already been reported.
  • FIGS. 1 to 4 show the results of comparison of the already isolated acetotractate synthase gene of tobacco, Arabidopsis, rapeseed, corn and the like with the amino acid sequence deduced from the nucleotide sequence of the gene of the present invention. Because of high homology with these, it can be said that the gene of the present invention contains the entire nucleotide sequence encoding acetotractate synthase.
  • the amino acid sequence deduced from this nucleotide sequence is simultaneously described in SEQ ID NO: 1 in the sequence listing.
  • the protein represented by the deduced amino acid sequence consists of 666 amino acid residues.
  • valine is present in the sensitive strain, while dalminic acid is present in the resistant strain.
  • Figures 1 to 4 show a comparison with the amino acids of herbicide-sensitive acetolactocinase, which has been identified in other plants.
  • Aspartic acid or daltamic acid is present at position ⁇ , and glycine is present in the resistant strain.
  • amino acid substitutions in the four amino acid-inserted regions are only in pests, where susceptible strains have palin and resistant strains have glutamic acid.
  • Glutamin is conserved at position ⁇ 5, but serine is present in resistant strains.
  • proline corresponding to amino acid SEQ ID NO: 189 shown in SEQ ID NO: 1 in the sequence listing was replaced with serine. (1992), vol. 100, 662—668), and the amino acid sequence shown in SEQ ID NO: 1 in the sequence listing
  • An example in which proline equivalent to 649 was substituted with asparagine to acquire resistance Jiro Hattori a 1. Mo 1. G En. Genet. (1992), vol. 232.
  • the amino acid to be substituted is not limited to one type of amino acid, but also to various amino acids other than the conserved amino acids. It has been shown to exhibit resistance (JP-A-63-71184, European Patent Publication No. 257993).
  • nagas are amino acids other than aspartic acid and glutamic acid, and preferably acquire resistance by being substituted with glycine.
  • Is an amino acid other than valine, and preferably obtains resistance by being replaced with glutamic acid.
  • a is an amino acid other than tributophan, and preferably acquires resistance by being substituted with arginine.
  • ⁇ 5 is an amino acid other than asparagine, and preferably obtains resistance by being replaced with serine.
  • the acetratractate synthase protein in which the amino acid of / 3, rS has been substituted with at least one other amino acid has resistance to a sulfonylprea herbicide.
  • the DNA sequence of the present invention can be identified based on the primary sequence of amino acid of acetotractate synthase and its functional characteristics. Furthermore, SEQ ID NO: in the sequence listing showing resistance to sulfonylrea herbicides A nucleic acid fragment encoding an acetate synthase consisting of 2300 bases represented by 1 can be provided.
  • the gene for acetolactate synthase resistant to a sulfonylprea herbicide of the present invention is prepared, for example, from mRNA of syrup which is resistant to a sulfonylprea herbicide, and then synthesizes cDNA. From the identified partial base sequence, the desired full-length gene can be isolated by PCR (Polymerase chain reaction) amplification or by screening a cDNA library. ?
  • PCR amplification of the acetate synthase gene involves extracting mRNA from plant tissues resistant to sulfonylrea, synthesizing cDNA using reverse transcriptase, and converting it into a double strand by the polymerase reaction.
  • the cDNA is linked to a synthetic DNA that functions as a primer used in the PCR method at both ends as an adapter.
  • the cDNA is type II, and the nucleotide sequence in the domain A region that has already been identified (for example, the sequence in the sequence listing).
  • PCR should be performed using, as a primer, a synthetic DNA containing the base sequence downstream of the domain A region (for example, the sequence shown in SEQ ID NO: 3) and the base sequence of the adapter. Can be amplified. Furthermore, the full-length gene can be isolated by cleaving the both with the restriction enzyme EcoRI between base sequence numbers 974 and 975 represented by SEQ ID NO: 1 in the sequence listing and ligating them.
  • a probe For screening of the cDNA library of the acetate reductase gene, for example, a probe may be prepared and, for example, plaque hybridization may be performed.
  • probes used for screening oligo DNA synthesized based on a partial sequence of the domain A region that has already been clarified, and the domain A region amplified by the PCR method and the DN of the target region A fragment can be used.
  • cDNA is synthesized using reverse transcriptase, and double-stranded by polymerase reaction.
  • a cDNA library can be prepared by cloning into one or plasmid vectors and transforming into E. coli or the like.
  • Various cDNA synthesis kits are commercially available and may be used.
  • a large number of vectors are commercially available for use in the production of libraries, and these can be used.
  • a primer was synthesized from the previously reported partial nucleotide sequence of domain A, and a plaque hybridization was carried out using a partial DNA fragment obtained by PCR amplification of a crude extract of the DNA of Bentaria as a probe.
  • a cDNA library is screened using a method such as a selection method, a clone containing DNA having complementarity with the probe is selected, DNA is prepared from the clone, and the clone is analyzed in detail to obtain an acetate synthase.
  • a cDNA clone containing the full-length gene can be obtained.
  • nucleotide sequence can be determined by the dideoxy method or the like, and a commercially available kit or a fluorescent sequencer can also be used.
  • a commercially available kit or a fluorescent sequencer can also be used.
  • the gene encoding the sulfonylurea-resistant acetolactate synthase of the present invention includes the full length, it is linked to, for example, the 35S promoter derived from the reflex mosaic virus or the ubiquitin gene promoter derived from maize.
  • a vector constructed so that the present gene is expressed in a plant it is possible to construct an expression system in the plant.
  • Figures 5 to 7 show an example of a method for constructing an expression vector.
  • Transformation of a plant susceptible to a sulfonylprea herbicide by using all or a part of the acetolactate synthase gene of the present invention including a protein translation region enables the resistance to a sulfonylprea herbicide. Can be provided.
  • all or a part of the acetate reductase synthase gene nucleic acid fragment of the present invention is sensitive to the sulfonylprea herbicide.
  • the resulting transformed cells are resistant to herbicides. Therefore, treatment with a selective concentration of a herbicide that suppresses the growth of untransformed cells is performed.
  • transformed cells can be selected from non-transformed cells.
  • a transformed plant cell susceptible to the drug shows resistance and allows the growth of untransformed cells to grow.
  • Transformed cells can be selected at a suppressed selective concentration, and they are used as a gene for selecting plant cells into which a target gene has been introduced.
  • the selection gene This is generally called the selection gene, but the sulfonylprea herbicide resistance gene can also be used as a selection marker when introducing the gene into plants (Zh ijian Liet. A 1. Plant Ph ysio 1. (1992), vol. 100, 662-668).
  • nucleic acid fragment obtained by combining the above-described nucleic acid fragment encoding an acettractate synthase that imparts herbicide resistance with a second nucleic acid fragment that imparts a second property, wherein the nucleic acid fragment transforms a plant.
  • the expression of herbicide resistance by the plant can be used as a marker for detecting the presence of the second nucleic acid fragment.
  • Tris-HCl (pH 7.5) was dissolved in 4 ml of an RNA solution of 1 mM EDTA, added to an oligo (dT) cellulose span column (Pharmacia), and adsorbed by shaking for 10 minutes. Wash 3 times with 3 ml of 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.5 M NaCl solution, 3 ml l OmM Tris-HC1 (pH 7.5) , LmM EDTA, ' / 3
  • c DNA synthesis was performed by reverse transcription using a Firrst-Strandc DNA Synthesis Kit (Pharmacia) and a Not I-d (T) 18 primer to obtain a 1 st strand c DN A was synthesized.
  • the I st strand c DNA solution was prepared using the Not I — d (T) 18 primer and the sequence shown in SEQ ID NO: 2 in the sequence listing (the sequence shown in base sequence numbers 553 to 582 shown in SEQ ID NO: 1) (5 'TCGTGC C CGGGTTTG Using a PA4 primer of CTGATGCTTTG CTCG 3 '), PCR amplification was performed with Tth DNA polymerase (manufactured by Toyobo) to amplify a fragment of about 1750 base. The obtained amplification fragment was digested with restriction enzymes SmaI and N0tI, and then subcloned into pBluescript SK (I) plasmid vector. A clone having the target insert of about 1750 base was screened to obtain a clone having a region 3 ′ downstream from nucleotide sequence number 561 shown in SEQ ID NO: 1.
  • a clone having a target insert of about 1000 bases was screened to obtain a clone having a region 5 'upstream from nucleotide sequence No. 979 shown in SEQ ID No. 1.
  • the cloned cDNA clone was used to obtain clones containing DNA fragments of various sizes by using KiLe-Sequence Deletion Kit (manufactured by Takara Shuzo).
  • the obtained clone was sequenced using Sequencing High-Cycle-Kit (manufactured by Toyobo Co., Ltd.) using the M13 Forward HT primer and T3 HT primer (manufactured by Toyobo Co., Ltd.), and the base was obtained. The arrangement was determined.
  • the obtained base sequence is shown as SEQ ID NO: 1 in the sequence listing.
  • FIGS. 1 to 4 show comparisons with known acetolactate synthases isolated from various plants, and the homology was recognized.
  • mRNA was extracted in the same manner as in Example 1, and 1st strand cDNA was synthesized.
  • a fragment of about 350 bp was amplified by PCR amplification with ⁇ th DNA polymerase.
  • the obtained amplification fragment was digested with restriction enzymes BamHI and XhoI, and then subcloned into pB1uescript SK (-) Plasmid.
  • a clone having the desired insert of about 350 bp was screened to obtain a clone having the 5 ′ terminal region of the tobacco ALS gene having the sequence shown in SEQ ID NO: 7 in the sequence listing.
  • a clone having a region from 553 to 3 ′ downstream of the nucleotide sequence shown in SEQ ID NO: 1 in the sequence listing of the susceptible acetate reductase gene A fragment obtained by digestion with Not I and restriction enzymes Mun I and N of a clone having a region 5 'upstream from 984 of the nucleotide sequence shown in SEQ ID NO: 1 of the acetate reductase gene
  • a full-length acetolactosynthase gene of a susceptible clover was constructed (Fig. 5). ).
  • a fragment obtained by digestion with the restriction enzymes EcoRI and NotI of a clone obtained by point-mutating the amino acid 570 of the amino acid SEQ ID NO: 1 shown in SEQ ID NO: 1 of the acetate reductase gene, and acetractate synthase The fragment obtained by digesting with a restriction enzyme EcoRI and NotI of a clone having a region 5 'upstream of 984 of the nucleotide sequence number shown in SEQ ID NO: 1 of p-Bluescript SK (-) plasmid
  • a full-length acetotractate synthase gene of the Japanese cypress having only four new mutations ( ⁇ , 7, and ⁇ ) was constructed.
  • Fragment obtained by digesting a clone having a region from 553 to 3 'downstream of the nucleotide sequence number shown in SEQ ID NO: 1 of the resistant acetactate synthase gene with restriction enzymes Ec ⁇ 22I and SacI The restriction enzymes EcoT22I and Sac of the clone containing the full-length acetate reductase synthase gene obtained by point mutation of 570 amino acids of the amino acid SEQ ID NO: 1 shown in SEQ ID NO: 1 of the acetate reductase gene.
  • the gene was inserted into a site cleaved with I to construct a full-length acetolactate synthase gene of resistant broomwood (Fig. 6).
  • the obtained clone was digested with restriction enzymes BamH I and SacI, and the fragment containing the full-length acetotractate synthase gene was transferred to a binary vector — PBI121 restriction enzymes BamHI and SacI. Inserted.
  • fragments obtained by digesting the clone having the 5 'terminal region of the tobacco ALS gene with the restriction enzymes BamHI and XhoI were used to express the above three types of full-length acetolactocin synthase genes in plants. Insertion into a site cleaved by the restriction enzymes BamHI and h011 allows the expression of three kinds of Asteracetate synthase genes in three pearl oysters having a region considered to be a tobacco chloroplast transport signal.
  • Agrobacterium LBA 4404 strain cells cultured in a ⁇ ⁇ medium were resuspended in a 2 OmM calcium chloride solution and used as a competent cell frozen and stored at -80 ° C.
  • Agrobacterium competent cell 100 jtz 1 was dissolved at room temperature, about 1 ⁇ g of the constructed expression vector was added, the mixture was incubated at 37 ° C for 5 minutes, and lml of LB medium was added. Later it was kept at 28 for 2 hours. The cells were collected as a pellet by centrifugation, resuspended in 100 ⁇ 1 LB medium, and used as an antibiotic (25 # g / ml kanamycin, 300 g / ml streptomycin).
  • An agrobacterium strain having an expression vector was transformed into an LB medium containing antibiotics (25 g / m1 kanamycin, 300 gZm1 streptomycin, 100 gm1 rifampicin) on an LB medium containing 28, Cultured for 24 hours. 5 mm square leaf sections of tobacco grown under aseptic conditions were immersed in the culture medium. After 5 minutes, the leaf sections were placed on sterile paper, excess medium was removed, and placed on MS-NB agar, 25. C, cultured under 3000 lux light. Three days later, leaf sections were transferred onto MS-NB agar medium containing 500 ⁇ g / ml claforan.
  • leaf sections were transferred to MS-NB agar medium supplemented with 100 # g / m 1 kanamycin and 500 / 3 ⁇ 4 gZml claforan, and transformants were selected.
  • the differentiated foliage of kanamycin resistance was transplanted to an MS medium containing 100 gZm1 of kanamycin.
  • the rooted plants were transplanted into pits and cultivated.
  • the transformant obtained from pBI-SH was designated as SH strain, the one derived from pBI-RH was designated as RH strain, and the one derived from pBI-RMH was designated as RMH strain.
  • Leaf slices (approximately 0.2 g) cut at intervals were mixed with 2 ml phosphate buffer (pH 6.0 10 mM potassium phosphate buffer, 0.0125% Triton X—100 50 ppm HOE—704, (In the presence or absence of chlorsulfuron) for 12 hours 24 hours under bright light conditions, and then ground with a Polytron homogenizer. This was centrifuged at 12,000 g for 10 minutes, the supernatant was removed, 0.1 ⁇ l of 6 ⁇ sulfuric acid was added, and the mixture was incubated at 60 ° C for 15 minutes.c Further, 5% of 1-naphthol was added.
  • 2 ml phosphate buffer pH 6.0 10 mM potassium phosphate buffer, 0.0125% Triton X—100 50 ppm HOE—704, (In the presence or absence of chlorsulfuron) for 12 hours 24 hours under bright light conditions, and then ground with a Polytron homogenizer. This was centrifuged at 1
  • Hoe 704 is a potent inhibitor of the enzyme ace t o 1 ac t a t e reduct i soierase.
  • R H 22 shares 100% 51% 34%
  • Acetractrates resistant to sulfonylprea herbicides according to the invention
  • a DNA sequence encoding a tosynthase is provided. This makes it possible to transform a plant, to impart resistance to a sulfonylurea herbicide to a plant, and to use it as a selection marker when introducing another gene. It becomes possible.
  • Sequence type nucleic acid
  • CTCCACACTC CTCTCTTTCA TTTTCTCTCT GATCATACCT TCAACCTTCA ACA ATG 56
  • Sequence type nucleic acid
  • Sequence type nucleic acid
  • Sequence type nucleic acid
  • Sequence type nucleic acid
  • Sequence type nucleic acid
  • Sequence type nucleic acid ⁇ Number of chains: double strand
  • Organism name tobacco (Ni cot i ana tabacum;

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Abstract

On décrit la séquence d'ADN d'un gène de l'acétolactate synthase résistant aux herbicides. La séquence d'ADN codant ce gène est décrite dans l'identification de séquence no. 1 de la liste des séquences. On peut par exemple obtenir ce gène à partir de l'ADN complémentaire de Kochia scoparia qui résiste aux herbicides à base de sulfonylurée.
PCT/JP1996/002448 1995-08-30 1996-08-30 Gene de l'acetolactate synthase resistant aux herbicides Ceased WO1997008327A1 (fr)

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JP7/222095 1995-08-30
JP22209595 1995-08-30

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WO1997008327A1 true WO1997008327A1 (fr) 1997-03-06

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017400B2 (en) 2005-05-09 2011-09-13 Kumiai Chemical Industry Co., Ltd. Method for transformation using mutant acetolactate synthase gene
JP2013542725A (ja) * 2010-10-15 2013-11-28 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー Ａｌｓインヒビター除草剤耐性ベータ・ブルガリス突然変異体

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6371184A (ja) * 1986-08-26 1988-03-31 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 除草剤抵抗性植物のアセトラクテ−トシンタ−ゼを暗号化する核酸断片

Patent Citations (1)

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JPS6371184A (ja) * 1986-08-26 1988-03-31 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 除草剤抵抗性植物のアセトラクテ−トシンタ−ゼを暗号化する核酸断片

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
MOL. GEN. GENET., 232(2), (1992), HATTORI JIRO et al., "Multiple Resistance to Sulfonylureas and Imidazolinones Cenferred by an Acetohydroxyacid Synthase Gene with S Mutation for Selective Resistance", p. 167-173. *
PLANT PHYSIOL., 100(2), (1992), LI ZHIJIAN et al., "A Sulfonylurea Herbicide Resistance Gene from Arabidopsis Thaliana as a New Selectable Marker for Production of Fertile Transgenic Rice Plants", p. 662-668. *
WEED SCI., 40(4), (1995), GUTTIERI MARY J. et al., "Diverse Mutations in the Acetolactate Synthase Gene Confer Chlorsulfuron Resistance in Kochia Biotypes", p. 175-178. *
WEED SCI., 41(2), (1993), SIVAKUMARAN K. et al., "Differential Herbicide Response Among Sulfonylurea-Resistant Kochia Scoparia L. Accessions", p. 159-165. *
WEED. SCI., 40(4), (1992), GUTTIERI MARY J. et al., "DNA Sequence Variation in Domain A of the Acetolactate Synthase Genes of Herbicide-Resistant and -Susceptible Weed Biotypes", p. 670-677. *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017400B2 (en) 2005-05-09 2011-09-13 Kumiai Chemical Industry Co., Ltd. Method for transformation using mutant acetolactate synthase gene
JP2013542725A (ja) * 2010-10-15 2013-11-28 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー Ａｌｓインヒビター除草剤耐性ベータ・ブルガリス突然変異体
JP2017108750A (ja) * 2010-10-15 2017-06-22 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ Ａｌｓインヒビター除草剤耐性ベータ・ブルガリス突然変異体
JP2018183168A (ja) * 2010-10-15 2018-11-22 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ Ａｌｓインヒビター除草剤耐性ベータ・ブルガリス突然変異体

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