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WO1997007220A3 - Linker insertion mutagenesis by means of the transposition system of insertion sequence is21 - Google Patents

Linker insertion mutagenesis by means of the transposition system of insertion sequence is21 Download PDF

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Publication number
WO1997007220A3
WO1997007220A3 PCT/EP1996/003625 EP9603625W WO9707220A3 WO 1997007220 A3 WO1997007220 A3 WO 1997007220A3 EP 9603625 W EP9603625 W EP 9603625W WO 9707220 A3 WO9707220 A3 WO 9707220A3
Authority
WO
WIPO (PCT)
Prior art keywords
linker
mutagenesis
transposition system
proteins
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1996/003625
Other languages
German (de)
French (fr)
Other versions
WO1997007220A2 (en
Inventor
Dieter Haas
Thomas Seitz
Bernard Berger
Sergio Schmid
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of WO1997007220A2 publication Critical patent/WO1997007220A2/en
Publication of WO1997007220A3 publication Critical patent/WO1997007220A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1018—Carboxy- and carbamoyl transferases (2.1.3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A genetic engineering process allows 4 to 11 codons to be inserted into any site of cloned genes. In genes that code for proteins, the reading pattern is not modified by an insertion. The insertions modify the protein structure, so that the proteins may be given a modified biologic activity. Transposition may be carried out according to this process both in vivo, for example in Escherichia coli, or in vitro. The process may also be used to insert epitopes.
PCT/EP1996/003625 1995-08-18 1996-08-16 Linker insertion mutagenesis by means of the transposition system of insertion sequence is21 Ceased WO1997007220A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH237395 1995-08-18
CH2373/95-3 1995-08-18

Publications (2)

Publication Number Publication Date
WO1997007220A2 WO1997007220A2 (en) 1997-02-27
WO1997007220A3 true WO1997007220A3 (en) 1997-03-20

Family

ID=4232259

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/003625 Ceased WO1997007220A2 (en) 1995-08-18 1996-08-16 Linker insertion mutagenesis by means of the transposition system of insertion sequence is21

Country Status (1)

Country Link
WO (1) WO1997007220A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2829986B1 (en) 2001-09-26 2003-12-26 Roulements Soc Nouvelle ELECTRIC POWER ASSISTED STEERING SYSTEM

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BACTERIAL CONJUGATION (CLEWELL, D., ed.), PLENUM PRESS, New York, 1993; Chapter 6; REIMMANN, C. & HAAS, D.:'Mobilization of chromosomes and nonconjugative plasmids bycointegrative mechanisms.' *
Diss. ETH Nr. 10197. ZÜRICH 1993. SCHMID, S.:'Die Insertionssequenz IS21: Entwicklung eines In-vitro Integrationssystems' *
HOEKSTRA, M. ET AL.: "A Tn3 derivative that can be used to make short in-frame insertions within genes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 88, June 1991 (1991-06-01), WASHINGTON US, pages 5457 - 5461, XP002024153 *

Also Published As

Publication number Publication date
WO1997007220A2 (en) 1997-02-27

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