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WO1992001814A3 - Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees - Google Patents

Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees Download PDF

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Publication number
WO1992001814A3
WO1992001814A3 PCT/US1991/005210 US9105210W WO9201814A3 WO 1992001814 A3 WO1992001814 A3 WO 1992001814A3 US 9105210 W US9105210 W US 9105210W WO 9201814 A3 WO9201814 A3 WO 9201814A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
amplification
reduction
specific amplification
vitro
Prior art date
Application number
PCT/US1991/005210
Other languages
English (en)
Other versions
WO1992001814A2 (fr
Inventor
John J Sninsky
David H Gelfand
Shirley Y Kwok
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to DK91916353T priority Critical patent/DK0540693T3/da
Priority to EP91916353A priority patent/EP0540693B1/fr
Priority to JP51560491A priority patent/JP3392863B2/ja
Priority to CA002087724A priority patent/CA2087724C/fr
Priority to AU85327/91A priority patent/AU665338B2/en
Priority to US07/960,362 priority patent/US5418149A/en
Priority to DE69130800T priority patent/DE69130800T2/de
Publication of WO1992001814A2 publication Critical patent/WO1992001814A2/fr
Publication of WO1992001814A3 publication Critical patent/WO1992001814A3/fr
Priority to GR990401066T priority patent/GR3029987T3/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Des procédés améliorés d'amplification d'acides nucléiques peuvent réduire l'amplification non-spécifique et minimiser les effets de contamination d'analyses de réactions d'amplification d'acide nucléique dus à des produits amplifiés provenant des précédentes amplifications. Ces procédés consistent à introduire des bases de nucléotides non conventionnelles dans les produits de réaction d'amplification et à traiter les produits au moyen d'éléments enzymatiques (par exemple des glycosylases) et/ou physiques-chimiques afin que le produit soit incapable d'agir comme une matrice dans des amplifications subséquentes.
PCT/US1991/005210 1990-07-24 1991-07-23 Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees WO1992001814A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DK91916353T DK0540693T3 (da) 1990-07-24 1991-07-23 Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
EP91916353A EP0540693B1 (fr) 1990-07-24 1991-07-23 REDUCTION D'AMPLIFICATION NON-SPECIFIQUE AU COURS D'UNE AMPLIFICATION $i(IN VITRO) D'ACIDE NUCLEIQUE UTILISANT DES BASES D'ACIDE NUCLEIQUE MODIFIEES
JP51560491A JP3392863B2 (ja) 1990-07-24 1991-07-23 修飾核酸塩基を使用するインビトロ核酸増幅における非特異的増幅の低減
CA002087724A CA2087724C (fr) 1990-07-24 1991-07-23 Reduction de l'amplification non specifique lors de l'amplification de l'acide nucleique in vitro grace a des bases d'acide nucleique modifiees
AU85327/91A AU665338B2 (en) 1990-07-24 1991-07-23 The reduction of non-specific amplification during (in vitro) nucleic acid amplification using modified nucleic acid bases
US07/960,362 US5418149A (en) 1990-07-24 1991-07-23 Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
DE69130800T DE69130800T2 (de) 1990-07-24 1991-07-23 Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
GR990401066T GR3029987T3 (en) 1990-07-24 1999-04-19 THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING -i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55751790A 1990-07-24 1990-07-24
US557,517 1990-07-24
US60915790A 1990-11-02 1990-11-02
US609,157 1990-11-02

Publications (2)

Publication Number Publication Date
WO1992001814A2 WO1992001814A2 (fr) 1992-02-06
WO1992001814A3 true WO1992001814A3 (fr) 1992-05-14

Family

ID=27071456

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/005210 WO1992001814A2 (fr) 1990-07-24 1991-07-23 Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees

Country Status (11)

Country Link
US (1) US5418149A (fr)
EP (1) EP0540693B1 (fr)
JP (1) JP3392863B2 (fr)
AT (1) ATE176002T1 (fr)
AU (1) AU665338B2 (fr)
CA (1) CA2087724C (fr)
DE (1) DE69130800T2 (fr)
DK (1) DK0540693T3 (fr)
ES (1) ES2128323T3 (fr)
GR (1) GR3029987T3 (fr)
WO (1) WO1992001814A2 (fr)

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US5418149A (en) 1995-05-23
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CA2087724C (fr) 2003-09-16
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AU8532791A (en) 1992-02-18
JPH06501612A (ja) 1994-02-24
ES2128323T3 (es) 1999-05-16
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JP3392863B2 (ja) 2003-03-31
ATE176002T1 (de) 1999-02-15

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