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WO1984002920A1 - Plantes transformees genetiquement - Google Patents

Plantes transformees genetiquement Download PDF

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Publication number
WO1984002920A1
WO1984002920A1 PCT/US1984/000050 US8400050W WO8402920A1 WO 1984002920 A1 WO1984002920 A1 WO 1984002920A1 US 8400050 W US8400050 W US 8400050W WO 8402920 A1 WO8402920 A1 WO 8402920A1
Authority
WO
WIPO (PCT)
Prior art keywords
plasmid
plant cells
gene
cells
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1984/000050
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English (en)
Inventor
Robert Thomas Fraley
Robert Bruce Horsch
Stephen Gary Rogers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23820643&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1984002920(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Monsanto Co filed Critical Monsanto Co
Priority to AT84900687T priority Critical patent/ATE66491T1/de
Priority to AU24363/84A priority patent/AU559562B2/en
Priority to DE8484900687T priority patent/DE3484947D1/de
Publication of WO1984002920A1 publication Critical patent/WO1984002920A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Definitions

  • pMON120 carries a gene (including a promoter) which codes for the expression of an enzyme, nopaline synthase (NOS). Once introduced into a plant cell, the NOS enzyme catalyzes the production of nopaline, a type of opine. In most types of plants, opines are non-detrimental compounds which accumulate at low levels; the presence of nopaline can be readily detected in plant tissue (Otten and Schilperoort, 1978). Opine genes may serve as useful marker genes to confirm transformation, since opines do not normally exist in untransformed plant cells.
  • the NOS gene in pMON120 may be rendered non-functional by a variety of techniques known to those skilled in the art. For example, a BamHI cleavage site exists within the coding portion of the NOS gene; a stop codon or other appropriate oligonucleotide sequence could be inserted into this site to prevent the translation of NOS.
  • Plasmid pMON90 was digested with Bglll, and a 2.6 kb fragment which contains the LIH region but not the left border. was purified. The 2.6 kb fragment was treated with Klenow polymerase to convert the cohesive ends into blunt ends, and the fragment was digested with MalawiI to obtain a 1.6 kb fragment (the desired fragment) and a 1 kb fragment. Both fragments were mixed with a pBR322 plasmid which had been digested with PvuII and Hindlll. The mixture was ligated, and inserted into E. coli cells. The cells were selected for ampicillin resistance, and scored for the presence of a Smal site which exists on the 1.6 kb fragment but not the 1 kb fragment. A colony having the desired plasmid was identified, and the plasmid from this colony was designated as pMON113, as indicated by Figure 6.
  • any gene for use in this invention may be determined through routine experimentation by those skilled in the art. Such usage is not limited to chimeric genes; for example, this invention may be used to insert multiple copies of a natural gene into plant cells.
  • This invention is suitable for use with a wide variety of plants, as may be determined through routine experimentation by those skilled in the art.
  • this invention is likely to be useful to transform cells from any type of plant which can be infected by bacteria from the genus Agrobacterium. It is believed that virtually all dicotyledonous plants, and certain monocots, can be infected by one or more strains of Agrobacterium.
  • microorganisms of the genus Rhizobia are likely to be useful for carrying co-integrate plasmids of this invention, as may be determined by those skilled in the art. Such bacteria might be preferred for certain types of transformations or plant types.
  • co-cultivation be utilized to transfer the co-integrate plasmids of this invention into plant cells.
  • a variety of other methods are being used to insert DNA into cells. Such methods include encapsulation of DNA in liposomes, complexing the DNA with chemicals such as polycationic substances or calcium phosphate, fusion of bacterial spheroplasts with plant protoplasts, microinjection of DNA into a cell, and induction of DNA uptake by means of electric current.
  • the purified 4.8 kb fragment (0.5 ug) was mixed with 0.3 ug of a 740 bp EcoRI-PstI fragment obtained from M13mp8 M-4 RF DNA (described in Example 2) which was purified using NA-45 membrane. Following ligation (T4 DNA ligase, 2 units), transformation of E. coli GM42 dam- cells, and selection for spectinomycin resistant cells, twenty colonies were obtained. Plasmid mini-prep DNA's prepared from twelve of these clones were digested with PstI and EcoRI to demonstrate the presence of the 740 bp fragment. One plasmid carrying this fragment was designated pMON64. A quantity of this plasmid DNA was prepared as described in Example 1.
  • Plasmid mini-preps were prepared from twelve colonies and digested with Hindlll to determine the size of the recombinant plasmid and with Smal to determine that the correct fragment had been inserted.
  • One plasmid with the correct structure was designated pMON113, as shown in Figure 6. Plasmid DNA was prepared as described in Example 1.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Des cellules végétales non affectées par des tumeurs sont transformées génétiquement. Un plasmide Ti (d'induction de tumeur) modifié est créé et contient un bord gauche du T-ADN (ADN de transfert), un ou plusieurs gènes désirés, et un bord droit de T-ADN. Cette région ne contient pas de gènes tumorigènes ou altérant la phytohormone. Le plasmide Ti est introduit dans les cellules végétales, où la région T-ADN est transférée dans le génome végétal. Les cellules végétales transformées peuvent être régénérées en plantes morphologiquement normales qui transmettront le(s) gène(s) désiré(s) à leur descendant.
PCT/US1984/000050 1983-01-17 1984-01-16 Plantes transformees genetiquement Ceased WO1984002920A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT84900687T ATE66491T1 (de) 1983-01-17 1984-01-16 Genetisch transformierte pflanzen.
AU24363/84A AU559562B2 (en) 1983-01-17 1984-01-16 Genetically transformed plants
DE8484900687T DE3484947D1 (de) 1983-01-17 1984-01-16 Genetisch transformierte pflanzen.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45840283A 1983-01-17 1983-01-17

Publications (1)

Publication Number Publication Date
WO1984002920A1 true WO1984002920A1 (fr) 1984-08-02

Family

ID=23820643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1984/000050 Ceased WO1984002920A1 (fr) 1983-01-17 1984-01-16 Plantes transformees genetiquement

Country Status (7)

Country Link
US (1) US8273954B1 (fr)
EP (1) EP0131620B1 (fr)
JP (1) JPS60500795A (fr)
AU (1) AU559562B2 (fr)
DE (1) DE3484947D1 (fr)
SU (1) SU1582990A3 (fr)
WO (1) WO1984002920A1 (fr)

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2159173A (en) * 1984-05-11 1985-11-27 Ciba Geigy Ag Transformation of hereditary material of plants
WO1986001225A1 (fr) * 1984-08-09 1986-02-27 Kabushiki Kaisha Advance Kaihatsu Kenkyujo Procede de traitement de cellules
EP0174166A1 (fr) * 1984-08-31 1986-03-12 Lubrizol Genetics Inc. T-ADN désarmé
EP0131624A4 (fr) * 1983-01-17 1986-08-21 Monsanto Co Plasmides de transformation de cellules vegetales.
EP0201904A1 (fr) * 1985-05-13 1986-11-20 Ciba-Geigy Ag Méthode de modification génétique de plantes
EP0200746A4 (fr) * 1984-10-01 1987-02-12 Gen Hospital Corp Cellules vegetales resistant a des inhibiteurs herbicides de la synthetase de glutamine.
WO1987005629A1 (fr) * 1986-03-11 1987-09-24 Plant Genetic Systems N.V. Cellules vegetales resistantes aux inhibiteurs de la glutamine-synthetase, obtenues par manipulation genetique
EP0239801A1 (fr) * 1986-02-27 1987-10-07 The General Hospital Corporation Cellules végétales résistantes aux inhibiteurs herbicides de la synthétase de glutamine
EP0233915A4 (fr) * 1985-07-29 1988-03-23 Calgene Inc Culture moleculaire.
EP0290455A4 (fr) * 1986-01-08 1989-01-18 Rhone Poulenc Agrochimie Gene decomposant l'haloarylnitrile, son utilisation et cellules le contenant.
GB2183660B (en) * 1985-11-22 1990-01-10 Ciba Geigy Ag Direct insertion of dna into plastids and mitochondria
EP0437320A1 (fr) * 1990-01-10 1991-07-17 Korea Institute Of Science And Technology ADN recombinant et son utilisation lors de la production d'insuline humaine
US5102796A (en) * 1983-04-15 1992-04-07 Lubrizol Genetics, Inc. Plant structural gene expression
US5231019A (en) * 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5317096A (en) * 1985-01-18 1994-05-31 Plant Genetic Systems, N.V. Transformation vectors allowing expression of foreign polypeptide endotoxins from Bacillus thuringiensis in plants
US5767376A (en) * 1995-06-07 1998-06-16 University Of Hawaii At Manoa Nucleic acids encoding a papaya ACC synthase gene
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) * 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
US6281410B1 (en) 1986-07-31 2001-08-28 Calgene Llc Methods and compositions for regulated transcription and expression of heterologous genes
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US6774283B1 (en) 1985-07-29 2004-08-10 Calgene Llc Molecular farming
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US7781648B2 (en) 2005-05-18 2010-08-24 Board Of Trustees Of Michigan State University Resistance to soybean aphid in early maturing soybean germplasm
US9133475B2 (en) 2008-11-26 2015-09-15 Board Of Trustees Of Michigan State University Aphid resistant soybean plants
US10253329B2 (en) 2013-01-04 2019-04-09 Board Of Trustees Of Michigan State University Sources of aphid resistance in soybean plants
CN110643566A (zh) * 2019-10-24 2020-01-03 福建省农业科学院水稻研究所 一种水稻原生质体分离与转化方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8300699A (nl) * 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NZ207765A (en) * 1983-04-15 1987-03-06 Lubrizol Genetics Inc Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp
NZ207766A (en) * 1983-04-15 1987-03-06 Lubrizol Genetics Inc Plant structural gene expression
NZ209338A (en) * 1983-09-14 1988-02-12 Lubrizol Genetics Inc Plasmid for the transformation of a plant cell
KR910007612B1 (ko) * 1990-01-10 1991-09-28 한국과학기술연구원 사람 인슈린 단백질을 생산하는 담배 및 그의 제조방법
ATE443437T1 (de) * 1995-10-13 2009-10-15 Dow Agrosciences Llc Modifiziertes bacillus thuringiensis gen zur kontrolle von lepidoptera in pflanzen
JP2004533807A (ja) 2000-11-07 2004-11-11 ノース・キャロライナ・ステイト・ユニヴァーシティ プトレッシン−ｎ−メチルトランスフェラーゼプロモーター
CN101611138B (zh) * 2006-12-29 2013-08-14 陶氏益农公司 用于作为具有完整细胞壁的悬浮单细胞的植物细胞系的诱导和维持的体外方法及其转化
US20130227746A1 (en) 2010-11-11 2013-08-29 Purdue Research Foundation Methods and compositions to regulate plant transformation susceptibility
US11236347B2 (en) 2015-08-28 2022-02-01 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
DE2942780A1 (de) 1979-10-23 1981-05-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Eukaryotische zellen, eukaryotische protoplasten und vielzellige eukaryotische lebende organismen mit einem gehalt an durch lipidvesikel eingebrachter dna, verfahren zu ihrer herstellung von gen-produkten, zur immunisierung und zur behebung genetisch bedingter defekte
FR2500847B1 (fr) 1981-03-02 1985-09-13 Pasteur Institut Marqueurs genetiques selectifs pour cellules eucaryotes, procede de mise en oeuvre de tels marqueurs et application des cellules contenant un tel marqueur a la fabrication de proteines determinees apres leur transformation par un adn correspondant
US4407956A (en) 1981-03-13 1983-10-04 The Regents Of The University Of California Cloned cauliflower mosaic virus DNA as a plant vehicle
CA1192510A (fr) 1981-05-27 1985-08-27 Lawrence E. Pelcher Vecteur de arn virale de virus des plantes ou portion de ce vecteur, methode de construction, et methode de production d'un gene derive
EP0090033A1 (fr) 1981-10-01 1983-10-05 International Plant Research Institute Procede de modification genetique de cereales avec des vecteurs de transformation
NL8200523A (nl) 1982-02-11 1983-09-01 Univ Leiden Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna.
US4436475A (en) 1982-02-16 1984-03-13 Rolligon Corporation Log skidder with load distributing boom attachment
US4459355A (en) 1982-07-12 1984-07-10 International Paper Company Method for transforming plant cells
IL69382A (en) * 1982-08-05 1991-01-31 Carter William Alvin Method of protecting plants against viral pathogens by inserting into plant cells genes encoding polypeptides containing interferon domains
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
US4536475A (en) * 1982-10-05 1985-08-20 Phytogen Plant vector
DE3382838T2 (de) * 1983-01-13 2004-04-15 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Transgene dicotyledone Pflanzenzellen und Pflanzen
EP0320500B1 (fr) * 1983-01-13 2004-11-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Système de vecteur plasmidique Ti non oncogène et molécules d'ADN recombinant pour l'introduction de gènes exprimables dans les gènomes de cellules végétales
US6051757A (en) 1983-01-14 2000-04-18 Washington University Regeneration of plants containing genetically engineered T-DNA
EP0131624B1 (fr) * 1983-01-17 1992-09-16 Monsanto Company Plasmides de transformation de cellules vegetales
US5352605A (en) * 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US6174724B1 (en) 1983-01-17 2001-01-16 Monsanto Company Chimeric genes suitable for expression in plant cells
US5034322A (en) * 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
JPH0714349B2 (ja) 1983-01-17 1995-02-22 モンサント カンパニ− 植物細胞での発現に適したキメラ遺伝子
NZ207765A (en) 1983-04-15 1987-03-06 Lubrizol Genetics Inc Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp
BR8404834A (pt) 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
DK162399C (da) 1986-01-28 1992-03-23 Danisco Fremgangsmaade til ekspression af gener i baelgplanteceller, dna-fragment, rekombineret dna-fragment samt plasmid til brug ved udoevelsen af fremgangsmaaden
US5106739A (en) * 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEEMANS et al, J. of Mol. Appl. Genet. Vol 1 No.2 pages 149-164 (1981) *
MATZKE et al, J. of Mol. Appl. Genet. Vol. No. 1 pages 39-49 (1981) *
OTTEN et al, Mol. Gen. Genet. Vol 183 pages 209-213 (1981) *
SCHELL et al, Genetic Engineering to Biotechnology- the Critical Transition Edited by W.J. WHELAN and Sandra published by JOHN WILEY and Sons LTD. pages 41-52. *

Cited By (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0131624A4 (fr) * 1983-01-17 1986-08-21 Monsanto Co Plasmides de transformation de cellules vegetales.
US5102796A (en) * 1983-04-15 1992-04-07 Lubrizol Genetics, Inc. Plant structural gene expression
US5591605A (en) * 1983-04-15 1997-01-07 Mycogen Plant Science, Inc. Plant structural gene expression
US6603065B2 (en) 1984-05-11 2003-08-05 Syngenta Invest Corp Transformation of hereditary material of plants
GB2159173A (en) * 1984-05-11 1985-11-27 Ciba Geigy Ag Transformation of hereditary material of plants
US5231019A (en) * 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US6201169B1 (en) 1984-05-11 2001-03-13 Novartis Finance Corporation Transformation of hereditary material of Brassica plants and cells
WO1986001225A1 (fr) * 1984-08-09 1986-02-27 Kabushiki Kaisha Advance Kaihatsu Kenkyujo Procede de traitement de cellules
EP0174166A1 (fr) * 1984-08-31 1986-03-12 Lubrizol Genetics Inc. T-ADN désarmé
EP0200746A4 (fr) * 1984-10-01 1987-02-12 Gen Hospital Corp Cellules vegetales resistant a des inhibiteurs herbicides de la synthetase de glutamine.
US5760181A (en) * 1985-01-18 1998-06-02 Plant Genetic Systems, N.V. Endotoxins
US5843898A (en) * 1985-01-18 1998-12-01 Plant Genetic Systems, N.V. Transformation vectors allowing expression of foreign polypeptide endotoxins in plants
US5317096A (en) * 1985-01-18 1994-05-31 Plant Genetic Systems, N.V. Transformation vectors allowing expression of foreign polypeptide endotoxins from Bacillus thuringiensis in plants
US5545565A (en) * 1985-01-18 1996-08-13 Plant Genetic Systems, N.V. Transformation vectors allowing expression of foreign polypeptide endoxins from Bacillus thuringiensis in plants
US6107546A (en) * 1985-01-18 2000-08-22 Aventis Cropscience N.V. Transformation vectors allowing expression of truncated BT2 endotoxins in plants
US5767372A (en) * 1985-01-18 1998-06-16 Plant Genetic Systems, N.V. Transformation vectors allowing expression of foreign polypeptide endotoxins from Bacillus thuringiensis in plants
EP0201904A1 (fr) * 1985-05-13 1986-11-20 Ciba-Geigy Ag Méthode de modification génétique de plantes
US6774283B1 (en) 1985-07-29 2004-08-10 Calgene Llc Molecular farming
US6096547A (en) * 1985-07-29 2000-08-01 Calgene, Llc Method and transgenic plant for producing mammalian peptides
US5629175A (en) * 1985-07-29 1997-05-13 Calgene, Inc. Molecular farming
EP0233915A4 (fr) * 1985-07-29 1988-03-23 Calgene Inc Culture moleculaire.
GB2183660B (en) * 1985-11-22 1990-01-10 Ciba Geigy Ag Direct insertion of dna into plastids and mitochondria
EP0229042A3 (fr) * 1986-01-08 1989-04-19 Rhone-Poulenc Agrochimie Gène de dégradation des nitriles haloanyliques, son utilisation, et cellules le contenant
EP0290455A4 (fr) * 1986-01-08 1989-01-18 Rhone Poulenc Agrochimie Gene decomposant l'haloarylnitrile, son utilisation et cellules le contenant.
EP0239801A1 (fr) * 1986-02-27 1987-10-07 The General Hospital Corporation Cellules végétales résistantes aux inhibiteurs herbicides de la synthétase de glutamine
US5648477A (en) * 1986-03-11 1997-07-15 Plant Genetic Systems, N.V. Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
WO1987005629A1 (fr) * 1986-03-11 1987-09-24 Plant Genetic Systems N.V. Cellules vegetales resistantes aux inhibiteurs de la glutamine-synthetase, obtenues par manipulation genetique
US5646024A (en) * 1986-03-11 1997-07-08 Plant Genetic Systems, N.V. Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
USRE44962E1 (en) 1986-03-11 2014-06-24 Bayer Cropscience N.V. Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
US5561236A (en) * 1986-03-11 1996-10-01 Plant Genetic Systems Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
US7112665B1 (en) 1986-03-11 2006-09-26 Bayer Bioscience N.V. Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
EP0242246A1 (fr) * 1986-03-11 1987-10-21 Plant Genetic Systems N.V. Cellules végétales résistantes aux inhibiteurs de la synthétase de glutamine, produites par génie génétique
EP0242236A1 (fr) * 1986-03-11 1987-10-21 Plant Genetic Systems N.V. Cellules végétales résistantes aux inhibiteurs de la synthétase de glutamine, produites par génie génétique
US6281410B1 (en) 1986-07-31 2001-08-28 Calgene Llc Methods and compositions for regulated transcription and expression of heterologous genes
EP0437320A1 (fr) * 1990-01-10 1991-07-17 Korea Institute Of Science And Technology ADN recombinant et son utilisation lors de la production d'insuline humaine
US5767376A (en) * 1995-06-07 1998-06-16 University Of Hawaii At Manoa Nucleic acids encoding a papaya ACC synthase gene
US7425670B2 (en) 1997-06-12 2008-09-16 North Carolina State University Methods and compositions for protein production in tobacco plants with reduced nicotine
US7605308B2 (en) 1997-06-12 2009-10-20 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) * 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
US6355860B1 (en) 1998-06-15 2002-03-12 Rutgers, The State University Of New Jersey Materials and methods for amplifying and enhanced transcribing of polynucleotides in plants and portions thereof
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
US7781648B2 (en) 2005-05-18 2010-08-24 Board Of Trustees Of Michigan State University Resistance to soybean aphid in early maturing soybean germplasm
US9133475B2 (en) 2008-11-26 2015-09-15 Board Of Trustees Of Michigan State University Aphid resistant soybean plants
US10253329B2 (en) 2013-01-04 2019-04-09 Board Of Trustees Of Michigan State University Sources of aphid resistance in soybean plants
CN110643566A (zh) * 2019-10-24 2020-01-03 福建省农业科学院水稻研究所 一种水稻原生质体分离与转化方法
CN110643566B (zh) * 2019-10-24 2022-09-02 福建省农业科学院水稻研究所 一种水稻原生质体分离与转化方法

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EP0131620A4 (fr) 1987-09-16
EP0131620A1 (fr) 1985-01-23
US8273954B1 (en) 2012-09-25
AU2436384A (en) 1984-08-15
SU1582990A3 (ru) 1990-07-30
DE3484947D1 (de) 1991-09-26
AU559562B2 (en) 1987-03-12
JPS60500795A (ja) 1985-05-30
EP0131620B1 (fr) 1991-08-21

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