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TR200200253T2 - Proteinlerin üretimi - Google Patents

Proteinlerin üretimi

Info

Publication number
TR200200253T2
TR200200253T2 TR2002/00253T TR200200253T TR200200253T2 TR 200200253 T2 TR200200253 T2 TR 200200253T2 TR 2002/00253 T TR2002/00253 T TR 2002/00253T TR 200200253 T TR200200253 T TR 200200253T TR 200200253 T2 TR200200253 T2 TR 200200253T2
Authority
TR
Turkey
Prior art keywords
heterologous polypeptide
npro
host cell
activity
coverage
Prior art date
Application number
TR2002/00253T
Other languages
English (en)
Inventor
Stempfer G�Nter
Windisch J�Rg
Knauseder Franz
Original Assignee
Bio-Chemie Gesellschaft M.B.H.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio-Chemie Gesellschaft M.B.H. filed Critical Bio-Chemie Gesellschaft M.B.H.
Publication of TR200200253T2 publication Critical patent/TR200200253T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Mevcut bulus bir bakteriyel konakçi hücrede açikça tanimli bir homojen N-terminusasahip arzu edilen bir heterolog polipeptidin rekombinan üretimi için bir proses ile ilgili olup, içerisindeki bir füzyon proteini ilkin, bir pestivirüsün bir toproteaz Npro'sunun otoproteolitik aktivitesine sahip bir peptit içermekte ve heterolog polipeptit, konakçi hücredeki sitoplazmik kapsama yapisi seklinde meydana getirilir ve kapsama yapilari izole edilir ve muameleye tabi tutulur ki arzu edilen heterolog polipeptit, füzyon proteininden Npro aktivitesi ile otoproteolitikal olarak klavlanir.
TR2002/00253T 1999-08-09 2000-08-07 Proteinlerin üretimi TR200200253T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT136799 1999-08-09

Publications (1)

Publication Number Publication Date
TR200200253T2 true TR200200253T2 (tr) 2002-05-21

Family

ID=3512357

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2002/00253T TR200200253T2 (tr) 1999-08-09 2000-08-07 Proteinlerin üretimi

Country Status (21)

Country Link
US (2) US6936455B1 (tr)
EP (1) EP1200604B1 (tr)
JP (1) JP4820512B2 (tr)
KR (1) KR20020026366A (tr)
CN (1) CN1230543C (tr)
AT (1) ATE335826T1 (tr)
AU (1) AU775662B2 (tr)
CA (1) CA2379571C (tr)
CZ (1) CZ302366B6 (tr)
DE (1) DE60029968T2 (tr)
ES (1) ES2269173T3 (tr)
HK (1) HK1047128B (tr)
HU (1) HU230231B1 (tr)
IL (1) IL147456A0 (tr)
MX (1) MXPA02001424A (tr)
NO (1) NO328339B1 (tr)
NZ (1) NZ516869A (tr)
PL (1) PL200225B1 (tr)
SK (1) SK287488B6 (tr)
TR (1) TR200200253T2 (tr)
WO (1) WO2001011057A1 (tr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL203708B1 (pl) * 1999-08-09 2009-11-30 Sandoz Ag Cząsteczka kwasu nukleinowego kodująca białko fuzyjne, wektor ekspresyjny zawierający tę cząsteczkę oraz komórka gospodarza bakteryjnego zawierająca ten wektor
EP2269589B1 (en) 2004-03-30 2013-12-25 Relypsa, Inc. Ion binding polymers and uses thereof
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
PT1874932E (pt) * 2005-04-26 2013-01-24 Sandoz Ag Produção de proteínas recombinantes por clivagem autoproteolítica de uma proteína de fusão
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
CN103069000A (zh) * 2010-08-31 2013-04-24 天野酶株式会社 使用了酶的蛋壳膜的溶解方法
EP2684951A1 (en) 2012-07-13 2014-01-15 Sandoz Ag Method for producing a recombinant protein of interest
EP2746391A1 (en) 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
EP2746390A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
ES2059482T3 (es) 1987-12-23 1994-11-16 Boehringer Ingelheim Int Expresion de la proteasa p2a de hrv2 codificada por virus.
JP4120972B2 (ja) * 1997-04-25 2008-07-16 セムバイオシス ジェネティックス インコーポレイテッド 融合蛋白質を開裂させる方法
EP1012307B1 (de) 1997-08-22 2005-05-04 Roche Diagnostics GmbH Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
WO1999010483A2 (de) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung

Also Published As

Publication number Publication date
AU775662B2 (en) 2004-08-12
EP1200604A1 (en) 2002-05-02
JP4820512B2 (ja) 2011-11-24
IL147456A0 (en) 2002-08-14
DE60029968D1 (de) 2006-09-21
US20050186564A1 (en) 2005-08-25
NO328339B1 (no) 2010-02-01
CN1230543C (zh) 2005-12-07
CA2379571C (en) 2011-03-29
HUP0202519A3 (en) 2004-07-28
EP1200604B1 (en) 2006-08-09
HU230231B1 (hu) 2015-10-28
CZ302366B6 (cs) 2011-04-13
PL353101A1 (en) 2003-10-20
AU6993300A (en) 2001-03-05
HK1047128A1 (en) 2003-02-07
NO20020633L (no) 2002-04-05
KR20020026366A (ko) 2002-04-09
CN1369014A (zh) 2002-09-11
WO2001011057A1 (en) 2001-02-15
DE60029968T2 (de) 2006-12-21
JP2003506093A (ja) 2003-02-18
NO20020633D0 (no) 2002-02-08
NZ516869A (en) 2004-01-30
HK1047128B (en) 2007-06-08
CZ2002481A3 (cs) 2002-05-15
HUP0202519A2 (en) 2002-10-28
MXPA02001424A (es) 2002-08-12
ATE335826T1 (de) 2006-09-15
US6936455B1 (en) 2005-08-30
CA2379571A1 (en) 2001-02-15
ES2269173T3 (es) 2007-04-01
SK287488B6 (sk) 2010-11-08
SK1852002A3 (en) 2002-07-02
PL200225B1 (pl) 2008-12-31

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