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MXPA02001424A - Produccion de proteinas. - Google Patents

Produccion de proteinas.

Info

Publication number
MXPA02001424A
MXPA02001424A MXPA02001424A MXPA02001424A MXPA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A MX PA02001424 A MXPA02001424 A MX PA02001424A
Authority
MX
Mexico
Prior art keywords
heterologous polypeptide
host cell
fusion protein
inclusion bodies
npro
Prior art date
Application number
MXPA02001424A
Other languages
English (en)
Inventor
Gunter Stempfer
Original Assignee
Biochemie Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochemie Gmbh filed Critical Biochemie Gmbh
Publication of MXPA02001424A publication Critical patent/MXPA02001424A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invencion se refiere a un proceso para la produccion recombinante de un polipeptido heterologo deseado con un termino N homogeneo claramente definido en una celula bacteriana hospedera, en donde inicialmente se forma una proteina de fusion que comprende un peptido con la actividad autoproteolitica de una autoproteasa Npro de un pestivirus y el polipeptido heterologo en la forma de cuerpos de inclusion citoplasmicos en la celula hospedera, y luego se aislan los cuerpos de inclusion y se tratan de tal manera que el polipeptido heterologo deseado se disocie autoproteoliticamente mediante la actividad de Npro, a partir de la proteina de fusion.
MXPA02001424A 1999-08-09 2000-08-07 Produccion de proteinas. MXPA02001424A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT136799 1999-08-09
PCT/EP2000/007643 WO2001011057A1 (en) 1999-08-09 2000-08-07 Production of proteins

Publications (1)

Publication Number Publication Date
MXPA02001424A true MXPA02001424A (es) 2002-08-12

Family

ID=3512357

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02001424A MXPA02001424A (es) 1999-08-09 2000-08-07 Produccion de proteinas.

Country Status (21)

Country Link
US (2) US6936455B1 (es)
EP (1) EP1200604B1 (es)
JP (1) JP4820512B2 (es)
KR (1) KR20020026366A (es)
CN (1) CN1230543C (es)
AT (1) ATE335826T1 (es)
AU (1) AU775662B2 (es)
CA (1) CA2379571C (es)
CZ (1) CZ302366B6 (es)
DE (1) DE60029968T2 (es)
ES (1) ES2269173T3 (es)
HK (1) HK1047128B (es)
HU (1) HU230231B1 (es)
IL (1) IL147456A0 (es)
MX (1) MXPA02001424A (es)
NO (1) NO328339B1 (es)
NZ (1) NZ516869A (es)
PL (1) PL200225B1 (es)
SK (1) SK287488B6 (es)
TR (1) TR200200253T2 (es)
WO (1) WO2001011057A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SK288290B6 (sk) * 1999-08-09 2015-07-01 Sandoz Ag Vektor na expresiu heterológnych proteínov v bakteriálnej hostiteľskej bunke, bakteriálna hostiteľská bunka a spôsob produkcie požadovaného heterológneho polypeptidu
KR101223335B1 (ko) 2004-03-30 2013-01-17 리립사, 인크. 이온 결합 조성물
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
PT1874825E (pt) 2005-04-26 2010-06-22 Sandoz Ag Ligandos de afinidade
CN105671018A (zh) * 2010-08-31 2016-06-15 天野酶制品株式会社 使用了酶的蛋壳膜的溶解方法
EP2684951A1 (en) 2012-07-13 2014-01-15 Sandoz Ag Method for producing a recombinant protein of interest
EP2746391A1 (en) 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
EP2746390A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
DE3885431D1 (de) 1987-12-23 1993-12-09 Boehringer Ingelheim Int Expression der viral kodierten Protease P2A des HRV2.
MX219629B (es) * 1997-04-25 2004-03-30 Sembiosys Genetics Inc Metodo para separar proteinas de fusion
ATE294869T1 (de) 1997-08-22 2005-05-15 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
AU9341398A (en) * 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Protease precursors that can be autocatalytically activated and their use

Also Published As

Publication number Publication date
US20050186564A1 (en) 2005-08-25
ATE335826T1 (de) 2006-09-15
CN1230543C (zh) 2005-12-07
ES2269173T3 (es) 2007-04-01
HUP0202519A2 (en) 2002-10-28
NO20020633L (no) 2002-04-05
DE60029968D1 (de) 2006-09-21
AU775662B2 (en) 2004-08-12
IL147456A0 (en) 2002-08-14
CA2379571A1 (en) 2001-02-15
EP1200604A1 (en) 2002-05-02
HK1047128B (en) 2007-06-08
CZ2002481A3 (cs) 2002-05-15
EP1200604B1 (en) 2006-08-09
NO328339B1 (no) 2010-02-01
NO20020633D0 (no) 2002-02-08
KR20020026366A (ko) 2002-04-09
DE60029968T2 (de) 2006-12-21
CN1369014A (zh) 2002-09-11
PL200225B1 (pl) 2008-12-31
AU6993300A (en) 2001-03-05
US6936455B1 (en) 2005-08-30
SK1852002A3 (en) 2002-07-02
WO2001011057A1 (en) 2001-02-15
SK287488B6 (sk) 2010-11-08
HUP0202519A3 (en) 2004-07-28
CZ302366B6 (cs) 2011-04-13
JP4820512B2 (ja) 2011-11-24
PL353101A1 (en) 2003-10-20
JP2003506093A (ja) 2003-02-18
NZ516869A (en) 2004-01-30
TR200200253T2 (tr) 2002-05-21
CA2379571C (en) 2011-03-29
HK1047128A1 (en) 2003-02-07
HU230231B1 (hu) 2015-10-28

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