SU545336A1 - Method for predicting chronic liver diseases - Google Patents
Method for predicting chronic liver diseasesInfo
- Publication number
- SU545336A1 SU545336A1 SU2182126A SU2182126A SU545336A1 SU 545336 A1 SU545336 A1 SU 545336A1 SU 2182126 A SU2182126 A SU 2182126A SU 2182126 A SU2182126 A SU 2182126A SU 545336 A1 SU545336 A1 SU 545336A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- chronic liver
- liver diseases
- growth
- predicting chronic
- predicting
- Prior art date
Links
- 238000000034 method Methods 0.000 title description 5
- 208000019423 liver disease Diseases 0.000 title description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000004393 prognosis Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000005229 liver cell Anatomy 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 241000021559 Dicerandra Species 0.000 description 1
- 235000010654 Melissa officinalis Nutrition 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical group COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000865 liniment Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003562 morphometric effect Effects 0.000 description 1
- 238000013425 morphometry Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
1one
Изобретение относитс к медицине, оно может найти применение дл ирогнозирвани хронических заболеваний печени.The invention relates to medicine, it can be used for the irognostication of chronic liver diseases.
Известен способ прогнозировани хронических заболеваинй печени, заключающийс в том, что берут биоитат печени, зафиксированный в специальные жпдкостп, готов т срез и микроскопическим путем изучают состо ние клеток печепи 1J. Однако известный способ не обеспечивает точпости и достоверности прогноза .There is a method for predicting chronic liver diseases, which consists in taking liver bioitat, recorded in a special train, preparing a cut and microscopically examining the state of cells 1J. However, the known method does not provide the accuracy and accuracy of the forecast.
Цель изобретени - повышение точности ирогноза. Эта цель достигаетс тем, что эксплантаты иечени больного культивируют в двух идентичных средах, в одну из которых ввод т австралийский антиген, ио окончанию культивировани сравнивают площади зон роста оргапных культур: увеличение площади зоны роста экснлантата, нодвергнутого воздействию антигена, свидетельствует о благопри тном прогнозе, отсутствие - о неблагопри тном .The purpose of the invention is to improve the accuracy of the horn. This goal is achieved by cultivating the patient's explants in two identical media, in one of which the Australian antigen is inserted, and by the end of the cultivation the areas of growth of orgap cultures are compared: an increase in the area of growth of the transplant exposed to the antigen indicates a favorable prognosis the absence is about unfavorable.
Способ осуществл ют следующим образом.The method is carried out as follows.
Биопсироваииую ткаиь печени больного забирают в стерильиую чашку Петри на стерильную салфетку. После ироиолаокивани биоптата в стерильном бюксе и разрезки его на отдельные фрагменты эксплантаты дел т па две части и каждую из них размещают иа миллипоровых фильтрах, расиоложенных над Biopsirovaya tkai liver of the patient is taken in a sterile Petri dish on a sterile napkin. After iriolization of the biopsy in a sterile tube and cutting it into separate fragments, the explants divide two parts and each of them is placed on a millipore filter placed above
отверсти ми колец из оргстекла, помещенных в чашки Конве . Оба чашки Конве заполн ют питательной средой до верхнего кра отверстий кольца.holes of plexiglass rings placed in conwe cups. Both Conwe cups are filled with nutrient medium to the upper edge of the holes in the ring.
Питательиую среду готов т следующим образом .The nutrient medium is prepared as follows.
Берут 60-70% среды 199, затем 30-40% сыворотки здорового донора АВ группы, 0,4% аскорбиновой кислоты и ио 100 ед. пенициллина и стрептомицина на 1 мл среды. В одну из чашек в питательную среду ввод т сыворотку, отмечавшую резко положптельную реакцию на австралийский антиген, интенсивностью три креста. Чашки Коиве герметически закрывают стекл нной крышкой с помощью смазки, состо щей из воска и вазелина. Предварительно внутрь чашки нагнетают из газометра смесь кислорода (65%), углекислого газа (5%) и воздуха (35%). Эксилантаты культнвируют ири 37°С на границе раздела газ-иитательиа среда в услови х, прпблилчеиных и условп м организма.They take 60-70% of medium 199, then 30-40% of serum of a healthy donor of the AV group, 0.4% of ascorbic acid, and io 100 units. penicillin and streptomycin in 1 ml of medium. In one of the cups, a serum was added to the nutrient medium, which marked a sharply positivity reaction to the Australian antigen, with an intensity of three crosses. Koive's cups are sealed with a glass lid using a lubricant consisting of wax and vaseline. Preliminary, a mixture of oxygen (65%), carbon dioxide (5%) and air (35%) is injected into the cup from the gasometer. The exciltants cultured at 37 ° C at the interface of the gas-imitative medium under the conditions and conditions of the organism.
По окоичании иериода культивировани биоптатов - через 20-30 суток фильтры с эксплаитатами фиксируют в течение 30 мин 96%-ным этанолом, крас т гематоксилином и заливают в бальзам. С помощью светового мпкроскоиа пропзвод т морфометрпческую оценку характера роста ткани печеии. С помощью микрометрической окул риой линейкиAfter circulating the iriod of biopsy cultivation, after 20-30 days, filters with exploits are fixed for 30 minutes with 96% ethanol, dyed with hematoxylin and poured into balm. Using a light microscopic method, morphometric evaluation of the nature of the growth of pechea tissue was made. With the help of the micrometer ruler
в случае необходимости производ т замер зон роста. Наход т соотношение площади зоны роста к площади эксплантата. Коэффициент контрол и опыта сравнивают между собой. При наличии стимул ции роста клеток печени в культуре, в питательную среду которой до культивировани был внесен австралийский антиген, устанавливают прогноз хронического заболевани печени удовлетворительным, а при отсутствии стимул ции роста клеток печени - неблагопри тным.if necessary, growth zones are measured. The ratio of the area of growth zone to the area of the explant is found. The coefficient of control and experience are compared with each other. In the presence of stimulation of the growth of liver cells in culture, in the nutrient medium of which the Australian antigen was introduced before culture, the prognosis of chronic liver disease is satisfactory, and in the absence of stimulation of the growth of liver cells, the prognosis is poor.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2182126A SU545336A1 (en) | 1975-10-17 | 1975-10-17 | Method for predicting chronic liver diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2182126A SU545336A1 (en) | 1975-10-17 | 1975-10-17 | Method for predicting chronic liver diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU545336A1 true SU545336A1 (en) | 1977-02-05 |
Family
ID=20634917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2182126A SU545336A1 (en) | 1975-10-17 | 1975-10-17 | Method for predicting chronic liver diseases |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU545336A1 (en) |
-
1975
- 1975-10-17 SU SU2182126A patent/SU545336A1/en active
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