SU1616898A1 - Method of producing disinfecting agent - Google Patents

Abstract

This invention relates to substituted guanidines, in particular to the preparation of a disinfectant. The goal is to improve the disinfecting properties of the target product. Receiving funds includes mixing hexamethylenediamine and guanidine hydrochloride and heating them. Hexamethylenediamine melt is uniformly introduced into the guanidine hydrochloride melt in a ratio of 1: (0.85-0.95) for 2.5 hours, while the mixture is heated to a temperature of 180 ° C, and after the introduction of hexamethylenediamine is completed, the temperature is raised to 240 ° C and maintain it for 5 hours. The method provides an improvement in the disinfecting properties by increasing the bactericidal action of the target product by 25 times while simultaneously reducing its toxicity by 3 times. 4 tab.

Description

The invention relates to organic chemistry, specifically to a method for producing a disinfectant based on polyhexamethyleneguanidine (PP1G), which is used in medicine and veterinary medicine, in particular in wastewater treatment.

The aim of the method is to improve the disinfecting properties of the target product.

Example 1. Pre-receive a melt of guanidine hydrochloride (GHC) as follows,

Dicyandiamide (DPDA.) In an amount of 0.85 kg and ammonium chloride (XA) in an amount of 1.15 kg are thoroughly mixed and placed in a 2-liter conical heat-resistant glass flask. The flask with the mixture is immersed in

oil bath with a temperature of 180 ° С. After 2-2.5 hours, the mixture is completely melted to form the product of the reaction, the melt guanidine hydrochloride. in the amount of 2.0 kg

In a stainless steel reactor with a capacity of 10 liters, where the temperature is maintained at 180 ° C, 2 kg of the liquid gas mixture is mixed. Melt hexamethylenediamine (1 KD) with a temperature of about 50 ° C (Tpl, 42 C) in an amount that ensures its molar ratio to HHI I, 0-0,85, evenly with constant stirring is introduced into the HHG melt during 2.5 h. Simultaneously with the introduction of the HMD, the mixture is heated to 80 s. At the end of the feed, the mixture is heated at a temperature rise to 24 () C and maintained for

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5 hours. At the same time, the mixture is continued to stir continuously.

PRI me R 2, the Method is carried out according to example F. The GMI is taken in an amount to ensure its relation to the GTH of 1.0-0.95,,

Example 3. The method is carried out according to example I, the GPA is taken in an amount ensuring its ratio to the GGH 1.0-0.95.

The addition of HMD to HGH in a molar ratio greater than 1.0-0.95 co-: is produced by the formation of a gel that clogs the apparatus. In this case: the target product loses its ability; it is soluble in water and cannot be used as a water-soluble disinfectant. The ratio of SD and GGH less than 1.0 g; |: 0.85 leads to a deterioration of the disinfectant; | curing properties of the target product, G. Table 1 presents the comparative: Some data on the synthesis and properties of the disinfectant obtained by proposed and known methods.

Elemental analysis of the compounds obtained in: Examples 1–4 is given in Table 2.

The data of elemental analysis of high molecular weight PHMG show the absence of destructive processes; when it is obtained by the method. The structure of the obtained funds (PSG) is confirmed by the results of IR spectroscopy.

Toxicity (LD jj) for warm-blooded PHMG obtained by the proposed method is 1500 mg / kg (LDD PGMG obtained by a known method, 300 mg / kg).

Determination of the antibacterial activity of the obtained disinfectant based on a PHMG at various ratios of GMD and GGH was carried out by the method of serial dilutions of the studied compounds on liquid nutrient media using m septon broth (MPB). To do this, in a series of pomeo tubes (2.0 ml of No. F each, then into the first tube — the same amount of test compound of a certain concentration (2500 µg / ml) from the first tube, after thorough mixing, 2.0 ml is transferred to the second tube and so on, with a two-fold dilution step to the 10th pro

Q

0 5

Q

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from 0

five

tags, 11-tube containing 2.0 ml alB is a control for the growth of the microorganism under study (Ps. aeruginosa),

After the compound has been diluted, a test microbial suspension is prepared, constituting the microbial cell load / ml. After instillation of a culture of 0.1 ml in each tube, a row of tripods is incubated for incubation for 18-24 h in a thermostat with a temperature of 37 ° C. After that, accounting for the growth of culture Ps is made. aeruginosa in test tubes and determine the minimum growth inhibitory microorganism concentration of the test compound.

The results are presented in table 3.

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I

The data of Table 3 shows that

The proposed range of ratios of GMI and HGH (, 85-0.95) provides an increase in the bacterial activity of the disinfectant produced by a factor of 2.

To compare the known and proposed bactericidal agents, the bactericidal indices of K. coli in water and toxicity are determined. In addition, the ability to infect a disinfectant is determined by the action of the target products on green algae in water.

The data obtained are given in table 4.

Thus, from the data presented in Table 4, it follows that this method, as compared with the prototype, provides an improvement in the disinfecting properties due to an increase (the Tactic acidity of the target product is 25 times while reducing its toxicity by three times.

Claims (1)

Invention Formula The method of obtaining a disinfectant by reacting hexamethylenediamine and the guanidine hydrochloride melt with heating, so that the hexamethylenediamine is melted beforehand and the process is carried out to improve the disinfecting properties of the target product. at a molar ratio of SRI of hexamethylenediamine and guanidiH hydrochloride 1: (O, 85–0.95) with a uniform addition of the obtained melt of hexamethylene diamine to the melt of guanidine hydrochloride for 2.5 hours, followed by increasing the temperature to and heating at this temperature for 5 hours. .t and l and U and I Molar ratio P 1D: GGH The proposed method 1: 0,9578,13 1: 0.90 78.13 1: 0.85. 78.13 1: 0.99; 156.25 The known method 1: 1. 156.25 Minimum inhibitory concentration on PS. aeru- ginosa, mg / ml Prototype 3000.5202, .0..11p 1.50 1.5 Table 4 ripped and decayed Cell growth 1, 8