SU1511274A1 - Method of determining activity of antibiotic and antiseptic substances - Google Patents
Method of determining activity of antibiotic and antiseptic substances Download PDFInfo
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- SU1511274A1 SU1511274A1 SU874197825A SU4197825A SU1511274A1 SU 1511274 A1 SU1511274 A1 SU 1511274A1 SU 874197825 A SU874197825 A SU 874197825A SU 4197825 A SU4197825 A SU 4197825A SU 1511274 A1 SU1511274 A1 SU 1511274A1
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- SU
- USSR - Soviet Union
- Prior art keywords
- culture
- incubation
- antibiotic
- carried out
- test culture
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000002421 anti-septic effect Effects 0.000 title claims description 6
- 230000000694 effects Effects 0.000 title claims 2
- 230000003115 biocidal effect Effects 0.000 title description 4
- 239000000126 substance Substances 0.000 title 1
- 229920001817 Agar Polymers 0.000 claims abstract description 6
- 239000008272 agar Substances 0.000 claims abstract description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 3
- 238000011534 incubation Methods 0.000 claims abstract 4
- 238000009792 diffusion process Methods 0.000 claims abstract 2
- 244000005700 microbiome Species 0.000 claims abstract 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 4
- 229940064004 antiseptic throat preparations Drugs 0.000 claims description 4
- 229940088710 antibiotic agent Drugs 0.000 claims description 3
- 230000005764 inhibitory process Effects 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 3
- 239000011780 sodium chloride Substances 0.000 abstract description 3
- 230000001133 acceleration Effects 0.000 abstract description 2
- 230000002906 microbiologic effect Effects 0.000 abstract description 2
- 230000017066 negative regulation of growth Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 238000010561 standard procedure Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 108010001478 Bacitracin Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 229960003071 bacitracin Drugs 0.000 description 2
- 229930184125 bacitracin Natural products 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940124350 antibacterial drug Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical compound OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 description 1
- 229960002064 kanamycin sulfate Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Изобретение относитс к медицинской микробиологии и микробиологической промышленности. Цель изобретени - ускорение способа. В способе в качестве тест-культуры микроорганизма используют штамм BACILLUS THERMONONLIQUEYACIENS ЦМПМ NB-2945. Исследуемый образец и культуру развод т в физиологическом растворе. Определение провод т традиционным методом диффузии в агар. Инкубирование посевов осуществл ют при температуре 60-65°С и учитывают результаты по зонам угнетени роста тест-культуры через 4-6 ч инкубации. Чувствительность способа на уровне стандартных методов. 1 табл.This invention relates to the medical microbiology and microbiological industry. The purpose of the invention is the acceleration of the method. In the method as a test culture of the microorganism used strain BACILLUS THERMONONLIQUEYACIENS CMPM NB-2945. The test sample and culture are diluted in saline. The determination is carried out by the traditional agar diffusion method. Incubation of crops is carried out at a temperature of 60-65 ° C and take into account the results on the zones of inhibition of growth of the test culture after 4-6 hours of incubation. The sensitivity of the method at the level of standard methods. 1 tab.
Description
Изобретение относитс к медицинской микробиологии и микробиологической промышленности, в частности к производству антибактериальных препаратов.This invention relates to the medical microbiology and microbiological industry, in particular to the manufacture of antibacterial drugs.
Цель изобретени - ускорение способа .The purpose of the invention is the acceleration of the method.
Способ осуществл ют следующим образом .The method is carried out as follows.
Культуру штамма ЦМПМ К В-.2945, хран щуюс на кос ках с МПА под вазелиновым маслом, высеваю т на чашки с МПА с добавлением 0,5% мелассы либо в колбы с ПМБ и культивируют 18 ч при 55-67°С. Выращенную культуру можно хранить в течение двух недель приThe culture of strain CMPM K B-.2945, stored on braids with MPA under vaseline oil, is sown on plates with MPA with the addition of 0.5% molasses or in flasks with PMB and cultured for 18 h at 55-67 ° C. Grown culture can be stored for two weeks at
. Дл выполнени анализа используют смыв в физиологическом растворе со свежего (ночного) высева на МПА. При этом питательный агар на чашки Петри разливают в два сло . Первый слой (20 мл стерильного агара) подсушивают при комнатной температуре в течение 2 ч, а затем разливают второй слой (5 мл агара, содержащего 1 X 10 клеток тест-культуры) при температуре не выше 55 С.. To perform the analysis, a flush in physiological solution from fresh (night) seeding on MPA is used. When this nutrient agar on Petri dishes poured into two layers. The first layer (20 ml of sterile agar) is dried at room temperature for 2 hours, and then the second layer (5 ml of agar containing 1 X 10 cells of the test culture) is poured at a temperature not higher than 55 C.
В застывшем агаре делают лун ки диаметром 8 мм и в них закапывают по 0,1 мл растворов стандартных препаратов и испытуемого образца. ЧашкиIn frozen agar, wells are made with a diameter of 8 mm and 0.1 ml of solutions of standard preparations and the test specimen are instilled into them. Cups
UU
31513151
инкубируют при 60-65°С в течение t- 6 м, после чего определ ют диаметр зон угнетени роста. Расчет провод т по предварительно построенной калибровочной кривой,incubated at 60-65 ° C for t-6 m, after which the diameter of growth inhibition zones is determined. The calculation is carried out according to a previously constructed calibration curve,
Пример 1. Определение баци- трацина в ветеринарном препарате бациллихин 30. Исходный препарат раствор ют в физиологическом растворе и готов т серию разведений.Example 1. Determination of bacitracin in the veterinary preparation bacilliquin 30. The initial preparation is dissolved in saline and a series of dilutions are prepared.
Полученные растворы закапывают в лунки питательного агара, зараженного Bacillus therraononliquefaciens ЦМПМ . Чашки инкубируют 6 ч при 65°Г,, затем определ ют диаметр зон задержки роста и рассчитывают концентрацию препарата по калибровочной кривой, построенной при использовании стандартных растворов с концентрацией 5-100 мкг/мл,The resulting solutions are instilled into the wells of nutrient agar infected with Bacillus therraononliquefaciens CMPM. The plates are incubated for 6 hours at 65 ° D, then the diameter of the growth inhibition zones is determined and the concentration of the preparation is calculated from a calibration curve constructed using standard solutions with a concentration of 5-100 µg / ml,
П р и м е р 2, Определение баци- трацина в культуральной жидкости.PRI me R 2, Determination of bacitracin in the culture fluid.
Ку ьтуральную жидкость центрифугируют дл отделени клеток продуцента (B.licbeni formis). Центрифугат развод т физиологическим раствором и затем провод т определение по примеру 1The cultural liquid is centrifuged to separate the producer cells (B.licbeni formis). Centrifugal diluted with saline and then determined as in Example 1
Биологическа активность культуральной жидкости составл ет 5200 мкг/ /мл (ю известному способу определено 5250 мкг/мл),The biological activity of the culture fluid is 5,200 µg / ml (5250 µg / ml is determined by a known method)
Пример ы 3 подгоговлен- ные чашки закапываю г растворы, Содержащие различные концентрации антибиотиков (канамицина сульфат, тетрацикExample 3 preconditioned cups. Instilled with solutions containing various concentrations of antibiotics (kanamycin sulfate, tetracyc
27s 27s
лин) или антисептиков (мертиолат, перекись водорода), по 0,1 мл на лунку. После ин кубации чашек при бО С вlin) or antiseptics (merthiolate, hydrogen peroxide), at 0.1 ml per well. After in the cubation of cups at C of C
течение 6 ч или при 65°С в течение U измер ют диаметры иигибировани зон роста бактерий, после чего с помощью калибровочных кривых определ ют концентрацию антибиотика или анти10 септика. Параллельно определ ют концентрацию антибиотикоЕ и антисептиков известным способом о Результаты представлены в таблице. for 6 hours or at 65 ° C for U, diameters and inhibition of the growth zones of bacteria are measured, after which the concentration of the antibiotic or anti-10 septic tank is determined using calibration curves. In parallel, the concentration of antibiotic and antiseptics is determined in a known manner. The results are presented in the table.
Таким образом, предлагаемый Спо15 соб позвол ет уменьшить врем , необходимое дл определени биологической активности или концентрации антибиоти- ка или антисептика, в 3- раза, без ухудшени точности определени по сравнению с известным способом.Thus, the proposed Method allows the time required to determine the biological activity or concentration of an antibiotic or antiseptic to be reduced by a factor of 3, without compromising the accuracy of the determination as compared with the known method.
2020
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU874197825A SU1511274A1 (en) | 1987-02-23 | 1987-02-23 | Method of determining activity of antibiotic and antiseptic substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU874197825A SU1511274A1 (en) | 1987-02-23 | 1987-02-23 | Method of determining activity of antibiotic and antiseptic substances |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1511274A1 true SU1511274A1 (en) | 1989-09-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU874197825A SU1511274A1 (en) | 1987-02-23 | 1987-02-23 | Method of determining activity of antibiotic and antiseptic substances |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1511274A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2424523C2 (en) * | 2009-06-09 | 2011-07-20 | ФГУ "Московский НИИ педиатрии и детской хирургии Росмедтехнологий" | Method of determining vancomycin concentration in biological fluids in newborns |
-
1987
- 1987-02-23 SU SU874197825A patent/SU1511274A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Андреев О.А. и др. Характеристика термофильных бактерий, выделенных из термальных источников Камчатки.. - Микробиологи , Т 52, 19бЗ, ti° 3, с.496-504. Егоров Н.С. Микробы-антагонисты и биологические методы определени антибиотической активности. М. Высша школа, 19б5, с.120-130. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2424523C2 (en) * | 2009-06-09 | 2011-07-20 | ФГУ "Московский НИИ педиатрии и детской хирургии Росмедтехнологий" | Method of determining vancomycin concentration in biological fluids in newborns |
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