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SG11201908777PA - Method for rapidly constructing amplicon library through one-step process - Google Patents

Method for rapidly constructing amplicon library through one-step process

Info

Publication number
SG11201908777PA
SG11201908777PA SG11201908777PA SG11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA
Authority
SG
Singapore
Prior art keywords
step process
amplicon library
rapidly constructing
constructing
rapidly
Prior art date
Application number
Other languages
English (en)
Inventor
Hai Yan
Sizhen Wang
Yuchen Jiao
Dayong Xu
qiaosong Zheng
Xiao Shi
Original Assignee
Genetron Health Beijing Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetron Health Beijing Co Ltd filed Critical Genetron Health Beijing Co Ltd
Publication of SG11201908777PA publication Critical patent/SG11201908777PA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201908777P 2017-04-05 2018-03-28 Method for rapidly constructing amplicon library through one-step process SG11201908777PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201710218529.4A CN106835292B (zh) 2017-04-05 2017-04-05 一步法快速构建扩增子文库的方法
PCT/CN2018/080864 WO2018184495A1 (zh) 2017-04-05 2018-03-28 一步法构建扩增子文库的方法

Publications (1)

Publication Number Publication Date
SG11201908777PA true SG11201908777PA (en) 2019-10-30

Family

ID=59142887

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201908777P SG11201908777PA (en) 2017-04-05 2018-03-28 Method for rapidly constructing amplicon library through one-step process

Country Status (7)

Country Link
US (1) US11155862B2 (zh)
EP (1) EP3608452A4 (zh)
JP (1) JP2020512405A (zh)
KR (1) KR102204274B1 (zh)
CN (1) CN106835292B (zh)
SG (1) SG11201908777PA (zh)
WO (1) WO2018184495A1 (zh)

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CN106835292B (zh) 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
CN107604045A (zh) * 2017-10-19 2018-01-19 北京泛生子基因科技有限公司 一种用于检测目的基因低频突变的扩增子文库的构建方法
CN112301430B (zh) * 2019-07-30 2022-05-17 北京泛生子基因科技有限公司 一种建库方法及应用
CN112322704B (zh) * 2020-12-02 2021-07-20 武汉艾迪晶生物科技有限公司 一种批量快速检测dna序列突变的方法
CN115233316A (zh) * 2021-04-25 2022-10-25 中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所 一种测序文库的制备方法及其应用
CN115233317A (zh) * 2021-04-25 2022-10-25 中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所 一种多重扩增子测序文库的制备方法及其应用
CN115247281A (zh) * 2021-04-25 2022-10-28 中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所 一种扩增子文库的制备方法及其应用
CN113308526B (zh) * 2021-07-13 2022-04-08 北京爱普益生物科技有限公司 融合引物直扩法人类线粒体全基因组高通量测序试剂盒
CN117661123A (zh) * 2023-12-02 2024-03-08 云准医药科技(广州)有限公司 一种多样品混合的单细胞建库方法
WO2025140356A1 (zh) * 2023-12-28 2025-07-03 南京诺唯赞生物科技股份有限公司 一种寡核苷酸集合以及多重扩增子建库方法

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SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
EP2559774A1 (en) * 2011-08-17 2013-02-20 Roche Diagniostics GmbH Improved method for amplification of target nucleic acids using a multi-primer approach
WO2014106076A2 (en) * 2012-12-28 2014-07-03 Quest Diagnostics Investments Incorporated Universal sanger sequencing from next-gen sequencing amplicons
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CN106319064A (zh) * 2016-09-13 2017-01-11 北京天科雅生物科技有限公司 一种基于高通量测序构建人tcra文库的多重pcr引物及方法
CN106834286B (zh) * 2017-04-05 2020-02-21 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的引物组合
CN106906210A (zh) * 2017-04-05 2017-06-30 北京泛生子医学检验实验室有限公司 一种快速构建扩增子文库的融合引物组合
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
CN107604045A (zh) * 2017-10-19 2018-01-19 北京泛生子基因科技有限公司 一种用于检测目的基因低频突变的扩增子文库的构建方法

Also Published As

Publication number Publication date
JP2020512405A (ja) 2020-04-23
KR102204274B1 (ko) 2021-01-15
EP3608452A4 (en) 2021-01-13
EP3608452A1 (en) 2020-02-12
WO2018184495A1 (zh) 2018-10-11
US20190352711A1 (en) 2019-11-21
CN106835292A (zh) 2017-06-13
KR20190104611A (ko) 2019-09-10
US11155862B2 (en) 2021-10-26
CN106835292B (zh) 2019-04-09

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