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SG11201903254PA - Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells - Google Patents

Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells

Info

Publication number
SG11201903254PA
SG11201903254PA SG11201903254PA SG11201903254PA SG11201903254PA SG 11201903254P A SG11201903254P A SG 11201903254PA SG 11201903254P A SG11201903254P A SG 11201903254PA SG 11201903254P A SG11201903254P A SG 11201903254PA SG 11201903254P A SG11201903254P A SG 11201903254PA
Authority
SG
Singapore
Prior art keywords
undifferentiated stem
cell
undifferentiated
removing agent
stem cell
Prior art date
Application number
SG11201903254PA
Inventor
Shugo Tohyama
Keiichi Fukuda
Jun Fujita
Sho Tanosaki
Original Assignee
Univ Keio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Keio filed Critical Univ Keio
Publication of SG11201903254PA publication Critical patent/SG11201903254PA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
    • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
    • A—HUMAN NECESSITIES
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/336—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having three-membered rings, e.g. oxirane, fumagillin
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    • A61K31/33—Heterocyclic compounds
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    • A61K31/365—Lactones
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4458—Non condensed piperidines, e.g. piperocaine only substituted in position 2, e.g. methylphenidate
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    • A61K31/7004—Monosaccharides having only carbon, hydrogen and oxygen atoms
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    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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Abstract

An undifferentiated stem cell-removing agent which contains at least one kind selected from the group consisting of a fatty acid synthesis inhibitor, a fatty acid 5 utilization inhibitor, and a cholesterol synthesis inhibitor; a method for removing undifferentiated stem cells which includes culturing a cell mixture that contains an undifferentiated stem cell and a differentiated cell in the presence of the undifferentiated stern cell-removing agent; and a production method of cells for transplantation which includes the following steps (i) and (ii): a step (i) of inducing a desired differentiated cell 10 from an undifferentiated stern cell, and a step (ii) of culturing the cell mixture obtained in the step (i) in the presence of the undifferentiated stem cell-removing agent are provided.
SG11201903254PA 2016-10-17 2017-10-17 Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells SG11201903254PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016203839 2016-10-17
PCT/JP2017/037495 WO2018074457A1 (en) 2016-10-17 2017-10-17 Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells

Publications (1)

Publication Number Publication Date
SG11201903254PA true SG11201903254PA (en) 2019-05-30

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Country Link
US (1) US20190300857A1 (en)
EP (1) EP3527657A4 (en)
JP (1) JP6811489B2 (en)
KR (1) KR102218042B1 (en)
CN (1) CN110050060A (en)
AU (1) AU2017346898B2 (en)
CA (1) CA3040637C (en)
IL (1) IL265980A (en)
RU (1) RU2735247C1 (en)
SG (1) SG11201903254PA (en)
WO (1) WO2018074457A1 (en)

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WO2018074457A1 (en) * 2016-10-17 2018-04-26 学校法人慶應義塾 Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells
CN111117951A (en) * 2018-10-30 2020-05-08 澳门大学 Method for regulating hPSC differentiation direction through IGF/insulin pathway and application
WO2020139914A1 (en) * 2018-12-26 2020-07-02 Academia Sinica Methods for regulating potency of pluripotent stem cells and applications thereof
KR102262557B1 (en) * 2020-01-10 2021-06-09 주식회사 히에라바이오 Method for improving functionality of cardiac stem cells
CN111979175A (en) * 2020-08-28 2020-11-24 安徽惠恩生物科技股份有限公司 Method for removing residual pluripotent stem cells in stem cell differentiation system
CN115927165B (en) * 2022-11-07 2025-06-27 武汉大学 Application of fatty acid anabolic pathway inhibitor in definitive endoderm cell differentiation
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