[go: up one dir, main page]

SG10201903910RA - Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same - Google Patents

Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same

Info

Publication number
SG10201903910RA
SG10201903910RA SG10201903910RA SG10201903910RA SG10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA SG 10201903910R A SG10201903910R A SG 10201903910RA
Authority
SG
Singapore
Prior art keywords
same
cell population
differentiation
induced cell
undifferentiated cells
Prior art date
Application number
SG10201903910RA
Inventor
Shigeo Masuda
Nagako Sougawa
Satsuki Fukushima
Shigeru Miyagawa
Yoshiki Sawa
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka filed Critical Univ Osaka
Publication of SG10201903910RA publication Critical patent/SG10201903910RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/25—Tumour necrosing factors [TNF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Transplantation (AREA)
  • Cardiology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

DIFFERENTIATION-INDUCED CELL POPULATION FROM WHICH UNDIFFERENTIATED CELLS HAVE BEEN REMOVED, USE OF SAME, AND METHOD FOR PRODUCING SAME 5 Provided is a cell population comprising differentiated cells obtainable by inducing differentiation of pluripotent stem cells, wherein the content ratio of undifferentiated pluripotent stem cells is 0.2% or less. 10 (No Suitable Figure)
SG10201903910RA 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same SG10201903910RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2014226682 2014-11-07

Publications (1)

Publication Number Publication Date
SG10201903910RA true SG10201903910RA (en) 2019-05-30

Family

ID=55909242

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201903910RA SG10201903910RA (en) 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
SG11201703544QA SG11201703544QA (en) 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201703544QA SG11201703544QA (en) 2014-11-07 2015-11-06 Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same

Country Status (9)

Country Link
US (2) US20170335284A1 (en)
EP (2) EP3567100A1 (en)
JP (1) JP6938154B2 (en)
KR (1) KR102639313B1 (en)
CN (1) CN107075473B (en)
AU (1) AU2015344125B2 (en)
CA (1) CA2966576C (en)
SG (2) SG10201903910RA (en)
WO (1) WO2016072519A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018014972A (en) * 2016-07-29 2018-02-01 国立大学法人大阪大学 Method for producing differentiation-inducing cell population from which undifferentiated cells are removed
JP7123809B2 (en) * 2016-12-20 2022-08-23 住友ファーマ株式会社 Removal agent for undifferentiated iPS cells
CN106854646A (en) * 2016-12-21 2017-06-16 南开大学 A kind of mouse embryo stem cell of the low expression Lin28 of maintenance mouse embryo stem cell Naive states
CN110506109B (en) * 2017-06-05 2024-03-12 泰尔茂株式会社 Cell culture manufacturing methods
JP7781521B2 (en) * 2018-09-27 2025-12-08 国立大学法人大阪大学 Method for producing a sheet of cells derived from pluripotent stem cells
WO2020067436A1 (en) * 2018-09-27 2020-04-02 国立大学法人大阪大学 Method for forming graft of pluripotent stem cell-derived cells
WO2020067438A1 (en) 2018-09-27 2020-04-02 国立大学法人大阪大学 Sheeting method for pluripotent stem cell-derived cells
WO2020102415A1 (en) * 2018-11-13 2020-05-22 Memorial Sloan-Kettering Cancer-Center Stem cell-based multiplex methods and compositions
WO2020166592A1 (en) 2019-02-13 2020-08-20 大日本住友製薬株式会社 Hemiasterlin derivative and antibody-drug conjugate thereof
US12312419B2 (en) 2019-02-13 2025-05-27 Sumitomo Pharma Co., Ltd. Hemiasterlin derivative having cysteine residue
US20220202948A1 (en) * 2019-02-13 2022-06-30 Sumitomo Dainippon Pharma Co., Ltd. Agent for Eliminating Pluripotent Stem Cells
KR102181316B1 (en) * 2019-05-31 2020-11-20 한국한의학연구원 Composition for inducing selective apoptosis undifferentiated human induced plurioptent stem cells comprising bee venom and the method for inducing selective apoptosis using the same
CN111979175A (en) * 2020-08-28 2020-11-24 安徽惠恩生物科技股份有限公司 Method for removing residual pluripotent stem cells in stem cell differentiation system
CN115074307B (en) * 2021-03-15 2024-01-09 广州暨南大学医药生物技术研究开发中心有限公司 Method for reprogramming fibroblast induced by small molecule compound into testis support cell and application thereof

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004217699B2 (en) * 2003-03-07 2008-07-03 Gamida-Cell Ltd. Expansion of renewable stem cell populations using modulators of PI 3-kinase
EP1983042B1 (en) * 2006-01-31 2017-03-15 Daiichi Sankyo Company, Limited A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
EP1994144B1 (en) * 2006-03-06 2017-11-22 Agency for Science, Technology and Research Human embryonic stem cell methods and podxl expression
US20090298169A1 (en) * 2006-06-02 2009-12-03 The University Of Georgia Research Foundation Pancreatic and Liver Endoderm Cells and Tissue by Differentiation of Definitive Endoderm Cells Obtained from Human Embryonic Stems
KR100937456B1 (en) * 2007-08-01 2010-01-19 한국생명공학연구원 How to differentiate human embryonic stem cells into osteoblasts
US20100035327A1 (en) * 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium
SG160248A1 (en) * 2008-09-18 2010-04-29 Agency Science Tech & Res Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells
US8415155B2 (en) * 2009-10-19 2013-04-09 Cellular Dynamics International, Inc. Cardiomyocyte production
WO2011094538A1 (en) * 2010-01-29 2011-08-04 The Board Of Trustees Of The Leland Stanford Junior University Depletion of teratoma-forming pluripotent stem cells
JP5913292B2 (en) * 2010-05-14 2016-04-27 ダナ−ファーバー キャンサー インスティテュート， インコーポレイテッド Compositions and methods for modulating metabolism
DK2571503T3 (en) * 2010-05-14 2015-04-20 Dana Farber Cancer Inst Inc COMPOSITIONS AND THEIR USE IN THE TREATMENT OF NEOPLASIA, INFLAMMATORY DISEASE AND OTHER DISORDERS
JP6039551B2 (en) * 2010-06-18 2016-12-07 セルラー ダイナミクス インターナショナル， インコーポレイテッド Cardiomyocyte culture medium with dialyzed serum
JPWO2012060461A1 (en) * 2010-11-02 2014-05-12 日本電気株式会社 Cooling device and manufacturing method thereof
RU2618871C2 (en) * 2011-06-09 2017-05-11 Ф. Хоффманн-Ля Рош Аг Method for pluripotent stem cells differentiation into vascular bed cells
CN104220458B (en) * 2012-02-24 2018-08-14 阿特根公司 It is combined with the modification antibody of the motif containing cysteine residues, the modification antibody-drug conjugates containing the modification antibody and its manufacturing method
WO2014058274A1 (en) * 2012-10-12 2014-04-17 서강대학교 산학협력단 Method for suppressing teratoma formation via selective cell death induction in undifferentiated human-induced pluripotent stem cells
CN114558032B (en) * 2012-12-21 2025-07-29 安斯泰来再生医药协会 Methods for producing platelets from pluripotent stem cells and compositions thereof

Also Published As

Publication number Publication date
AU2015344125A1 (en) 2017-05-25
WO2016072519A1 (en) 2016-05-12
EP3567100A1 (en) 2019-11-13
AU2015344125B2 (en) 2022-03-24
CN107075473A (en) 2017-08-18
KR102639313B1 (en) 2024-02-20
EP3216859A1 (en) 2017-09-13
EP3216859B1 (en) 2020-03-11
US20210388320A1 (en) 2021-12-16
SG11201703544QA (en) 2017-06-29
CA2966576A1 (en) 2016-05-12
CA2966576C (en) 2021-07-20
CN107075473B (en) 2021-07-13
JP6938154B2 (en) 2021-09-22
EP3216859A4 (en) 2017-09-13
KR20170074996A (en) 2017-06-30
JPWO2016072519A1 (en) 2017-08-17
US20170335284A1 (en) 2017-11-23

Similar Documents

Publication Publication Date Title
SG10201903910RA (en) Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
MY191740A (en) Production method for nerve tissue
GB2548740A (en) Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
TW201612312A (en) Meristematic plant cells and method of isolating them
WO2016061071A8 (en) Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures
MY183058A (en) Production method for retinal tissue
MX2016005658A (en) Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.
EP3161127A4 (en) Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof
EP3145517A4 (en) Functional oligodendrocytes derived from pluripotent stem cells and methods of making and using the same
MX368780B (en) Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof.
EP3263697A4 (en) Culture medium for culturing mesenchymal stem cell, method for culturing mesenchymal stem cell, and mesenchymal stem cell
EP3369809A4 (en) Method for obtaining leydig cells by carrying out induced differentiation on human induced pluripotent stem cells, and use of leydig cells
EP3124602A4 (en) Culture medium for pluripotent stem cells
EP3587559A4 (en) Production method for artificial pluripotent stem cells
AU2015321438A8 (en) Method for transforming a plant cell or plant tissue using Agrobacterium, transgenic plant, transgenic cell or transgenic tissue, culture medium and use of a method for transforming a plant cell or tissue
EP3196297A4 (en) Method for producing cardiomyocyte population from pluripotent stem cells
EP3202911A4 (en) Method for determining undifferentiated state of pluripotent stem cells by culture medium analysis
PL3436568T3 (en) Culture medium for pluripotent stem cells
MY181935A (en) Method for rupture of algae
EP3597734A4 (en) Method for producing helper t cells from pluripotent stem cells
EP3321356A4 (en) Mesenchymal stem cell, clonogenic amplification method thereof, separation method thereof and uses thereof
KR101896803B9 (en) Method for increasing the rate of inducing differentiation of human pluripotent stem cells to mesenchymal stem cells and mesenchymal stem cells produced by thereof
EP3156481A4 (en) Method for manufacturing induced pluripotent stem cells from adipose-derived mesenchymal stem cells and induced pluripotent stem cells manufactured by same method
EP3467103A4 (en) Method for producing neural stem cells, medium, supplement, supplement set, medium kit, and cell culture device
SG11201900989QA (en) Method for inducing differentiation of pluripotent stem cells in vitro