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SG11201809400UA - Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor - Google Patents

Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor

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Publication number
SG11201809400UA
SG11201809400UA SG11201809400UA SG11201809400UA SG11201809400UA SG 11201809400U A SG11201809400U A SG 11201809400UA SG 11201809400U A SG11201809400U A SG 11201809400UA SG 11201809400U A SG11201809400U A SG 11201809400UA SG 11201809400U A SG11201809400U A SG 11201809400UA
Authority
SG
Singapore
Prior art keywords
pluripotent stem
stem cells
pancreatic progenitor
cells derived
cells
Prior art date
Application number
SG11201809400UA
Inventor
Hiroo Iwata
Shuhei Konagaya
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of SG11201809400UA publication Critical patent/SG11201809400UA/en

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURIFICATION METHOD FOR PANCREATIC PRECURSOR CELLS DERIVED FROM PLURIPOTENT STEM CELLS AND AMPLIFICATION METHOD THEREFOR Disclosed are a method for culturing pancreatic progenitor cells derived from pluripotent stem cells, the method comprising step (A) of three-dimensionally culturing pancreatic progenitor cells derived from pluripotent stem cells in a medium containing a factor belonging to the epidermal growth factor (EGF) family and/or a factor belonging to the fibroblast growth factor (FGF) family, and (2) a Wnt agonist; a method for producing islet cells from pancreatic progenitor cells derived from pluripotent stem cells, the method comprising step (E) of inducing the differentiation of pancreatic progenitor cells cultured by the above method into islet cells; and a method for cryopreserving pancreatic progenitor cells derived from pluripotent stem cells, the method comprising step (F) of freezing pancreatic progenitor cells cultured by the above method. (No drawing to be published)
SG11201809400UA 2016-04-28 2017-04-27 Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor SG11201809400UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016091116 2016-04-28
PCT/JP2017/016728 WO2017188378A1 (en) 2016-04-28 2017-04-27 Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor

Publications (1)

Publication Number Publication Date
SG11201809400UA true SG11201809400UA (en) 2018-11-29

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ID=60159764

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SG11201809400UA SG11201809400UA (en) 2016-04-28 2017-04-27 Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor

Country Status (13)

Country Link
US (1) US20190136197A1 (en)
EP (1) EP3450541A4 (en)
JP (1) JP7055740B2 (en)
KR (1) KR102257868B1 (en)
CN (1) CN109415689A (en)
AU (1) AU2017255099B2 (en)
CA (1) CA3022365A1 (en)
EA (1) EA201892447A1 (en)
IL (1) IL262554A (en)
MX (1) MX2018013175A (en)
SG (1) SG11201809400UA (en)
TW (1) TWI710636B (en)
WO (1) WO2017188378A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111246864A (en) * 2017-07-21 2020-06-05 森玛治疗公司 Repopulation of stem cell-derived pancreatic beta cells
CA3108275A1 (en) 2018-08-10 2020-02-13 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
WO2020080550A1 (en) * 2018-10-15 2020-04-23 Cynity株式会社 Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ
CN109749986B (en) * 2019-03-13 2021-04-02 武汉大学 Method for obtaining pancreatic precursor cells and pancreatic beta cells by differentiation of human pluripotent stem cells
JP2022528737A (en) * 2019-04-01 2022-06-15 ステムサイト オイ How to Obtain or Maintain ABCG2-Positive Corneal Ring Stem Cells
CA3167649A1 (en) 2020-01-21 2021-07-29 Takeda Pharmaceutical Company Limited Compositions and methods of cryopreserving cells
CN112980774B (en) * 2021-03-19 2022-04-01 上海爱萨尔生物科技有限公司 Culture method for preparing islet beta cells by inducing pluripotent stem cells to differentiate directionally
CN112961823B (en) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 A culture medium for preparing pancreatic islet beta cells through directed differentiation of induced pluripotent stem cells
CN113046299B (en) * 2021-03-19 2024-06-28 上海爱萨尔生物科技有限公司 An additive for preparing pancreatic β cells by induced pluripotent stem cell directed differentiation
CN113234664B (en) * 2021-05-11 2024-05-10 澳门大学 Preparation method and application of pancreatic progenitor cells
CN115011544B (en) * 2022-05-30 2023-11-17 广州国家实验室 Method for obtaining islet delta cells by in vitro induction and application thereof
CN120330136B (en) * 2025-06-13 2025-10-03 南京兰伯艾克斯生物科技有限公司 A stem cell cloning culture method and serum-free culture medium

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JPS5579281A (en) 1978-11-28 1980-06-14 Nippon Oil Co Ltd Rainnproof construction of floatinggroof tank
EP2421957B1 (en) 2009-04-22 2020-11-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
EP2857500B1 (en) * 2012-05-25 2018-01-31 Saitama Medical University Method for producing pancreatic hormone-producing cell, pancreatic hormone-producing cell, and differentiation/induction promoter
JP2014161257A (en) * 2013-02-22 2014-09-08 Univ Of Tokyo Method for preparing pancreatic langerhans islet from pluripotent stem cell
WO2014165663A1 (en) * 2013-04-03 2014-10-09 Cellular Dynamics International, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
WO2015013653A1 (en) 2013-07-26 2015-01-29 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Generating definitive endoderm and pancreatic progenitor cells
SG11201700874SA (en) * 2014-08-04 2017-03-30 Takeda Pharmaceuticals Co Method for proliferation of pancreatic progenitor cells

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Publication number Publication date
JPWO2017188378A1 (en) 2019-04-04
MX2018013175A (en) 2019-02-21
EP3450541A1 (en) 2019-03-06
EA201892447A1 (en) 2019-04-30
KR102257868B1 (en) 2021-05-27
US20190136197A1 (en) 2019-05-09
JP7055740B2 (en) 2022-04-18
CN109415689A (en) 2019-03-01
KR20180136544A (en) 2018-12-24
AU2017255099B2 (en) 2021-07-15
TWI710636B (en) 2020-11-21
WO2017188378A1 (en) 2017-11-02
EP3450541A4 (en) 2019-11-13
CA3022365A1 (en) 2017-11-02
TW201800576A (en) 2018-01-01
AU2017255099A1 (en) 2018-11-22
IL262554A (en) 2018-12-31

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