RU2823288C1 - Method of improving amino acid composition of rabbit meat with simultaneous prevention of oxidative stress - Google Patents

Abstract

FIELD: animal breeding.

SUBSTANCE: invention relates to animal breeding and veterinary science and concerns a method for increasing biological value of rabbit meat with simultaneous prevention of microelementosis. Method involves feeding young stock twice with water containing Ferropeptide in dose of 0.5 ml/kg of animal weight daily for 2–3 months. That is combined with oral administration of Abiopeptide with fodder in dose of 1 ml/kg of body weight daily from 10-day to 60-day age.

EFFECT: invention makes it possible to increase content of a number of amino acids in rabbit meat, as well as to prevent development of microelementosis in rabbits.

1 cl, 2 tbl

Description

The invention relates to the field of animal husbandry and veterinary medicine and concerns a method for improving the amino acid composition of rabbit meat while simultaneously preventing oxidative stress in them.

Rabbit meat is a valuable product for agriculture and animal husbandry, as it has a number of advantages over the meat of other animal species.

From an agricultural perspective, rabbit meat is high in protein and low in fat and is easily digestible by the body. In addition, it is rich in vitamins and minerals such as iron, zinc, copper, cobalt, iodine, selenium, etc.

Rabbits can be raised indoors, allowing their nutrition and health to be monitored and the impact on the environment to be minimized.

From the point of view of animal husbandry, rabbits are characterized by high fertility, rapid growth and relatively low maintenance costs.

Rabbit meat can be used to produce various types of products, such as sausages, semi-finished products, canned food, etc. In addition, furs can be obtained from rabbit skins, which are used in the production of clothing, shoes and accessories.

Microelementoses in rabbits are a serious problem for agriculture and animal husbandry, as they can lead to a decrease in the productivity and quality of animal meat. Micronutrient deficiencies in rabbits can lead to various diseases and health problems, such as anemia, problems with growth and development, and decreased immunity. Excess trace elements can also cause negative consequences and lead to toxic effects in the animal's body.

Based on the foregoing, it can be argued that livestock farming is one of the most promising areas of agriculture in Russia, but requires the introduction of modern effective ways to increase production volumes.

There is a known method for improving the amino acid composition of rabbit meat while simultaneously preventing oxidative stress in them (RU 2800984 C1). The method involves oral administration of the feed additive to rabbits with food at a dose of 1 ml/kg of live body weight, as well as twice the administration of the feed additive "Nitamin" with water for drinking at a dose of 1.0 ml/kg of live body weight with an interval of 1 month at 30 -day and 60 days of age.

There is also a known method for increasing the productivity of farm animals and improving product quality (RU 2002111437 A). The proposed method includes in the diet a probiotic preparation containing lactic acid bacteria, but with the peculiarity that a recombinant strain of Lactobacillus sp. is used. 8RAZ (pLF-SL2), which promotes the formation of somatoliberin (growth hormone releasing factor) in the digestive tract.

The advantage of our method is that it allows us to increase the biological value of rabbit meat and prevent microelementosis by optimizing the diet with the introduction of feed additives containing amino acids and essential microelements, and regular monitoring of the health status of animals.

The objective of the invention is to develop a comprehensive strategy aimed at increasing the biological value of rabbit meat and preventing microelementosis in these animals.

The technical result of the claimed invention is to increase the biological value of rabbit meat, as well as to prevent the development of microelementosis.

To improve the biological value of rabbit meat while simultaneously preventing microelementosis in them, feed additives “Ferropeptide” and “Abiopeptid” (manufactured by Firm A-BIO LLC, Moscow region) were used.

1 liter of the food ingredient “Ferropeptide” contains as an active ingredient:

- Iron Fe(III) - 5-6 g;

- copper (Cu) - 0.5-0.6 g;

- cobalt (Co) - 0.03-0.4 g;

- selenium (Se) - 0.07-0.9 g;

- iodogorgonic acid (C9H9I2NO3) - 0.35-0.45 g;

- manganese (Mn) - 0.6-0.7 g;

- zinc (Zn) - 0.6-0.7 g;

- enzymatic hydrolyzate of soy protein (full complex of amino acids and peptides) - 100-150 g.

Excipients:

- Methyl hydroxybenzoate - 1.5 g;

- propyl hydroxybenzoate - 0.15 g;

- sodium citrate - 6 g;

- sucrose - 10 g;

- sorbitol - 14 g;

- drinking water - up to 1.0 l.

1 liter of feed additive "Abiopeptide" contains as an active ingredient: Enzymatic hydrolyzate of highly digestible vegetable protein - 250 g (25%).

As auxiliary components: potassium sorbate – 2.6 g (2.6%), water – up to 1 liter. The additive contains: total nitrogen – 3200 mg%, nitrogen of free amino groups of amino acids and peptides – 1500 mg%.

The specified technical result is achieved as follows. To improve the biological value of rabbit meat while simultaneously preventing microelementosis in them, young animals are fed twice with water containing Ferropeptide at a dose of 0.5 ml/kg of animal weight daily for 2-3 months. In parallel with this, “Abiopeptide” is administered orally with food at a dose of 1 ml/kg of live body weight daily from 10 days to 60 days of age.

As a result of the experiments, the high efficiency of the developed method was proven. For this purpose, 2 groups of Soviet chinchilla rabbits of 10 heads each were formed. The first group was the control group, no additional administration of drugs and feed additives was carried out, the animals of the second group (experimental) were given water containing Ferropeptide to the young animals at a dose of 0.5 ml/kg of animal weight daily for 2-3 months. In parallel with this, “Abiopeptide” is administered orally with food at a dose of 1 ml/kg of live body weight daily from 10 days to 60 days of age. All animals were kept under the same optimal conditions in standard cages. On the 85th day of the experiment, a blood test was performed to determine the concentration of iron, copper, cobalt, selenium, manganese, and zinc. Rabbits were slaughtered at the age of 90 days. The amino acid composition of rabbit meat was determined according to GOST 34132-2017 Meat and meat products. Method for determining the amino acid composition of animal protein.

The results of the study are presented in tables 1-2.

Table 1
The content of microelements in the blood of rabbits of the Soviet chinchilla breed Test group Indicator name Iron, µmol/l Copper, µmol/l Cobalt, nmol/l Manganese, µmol/l Zinc, µmol/l Control 30.1±2.1 28.6±1.76 509±0.09 88.42±0.02 204.25±0.002 Experience 19.6±2.45* 16.0±2.01 400±0.08* 85.9±0.06 190±0.02 *p < 0.05

According to Table 1, it can be seen that the studied indicators indicating microelementosis are higher in rabbits of the control group, which were not given feed additives. Thus, in the experimental group, the blood levels of iron were significantly normal at 10.5 µmol/l and copper at 12.6 µmol/l, which indicates the absence of microelementosis.

table 2
Amino acid composition of Soviet chinchilla rabbit meat No. Indicator name Test sample: meat Control Experience Alanine, % 1.2±0.3 1.8±0.3* Arginine, % 1.2±0.5 1.6±0.4 Valine, % 0.8±0.3 0.9±0.3 Histidine, % 0.5±0.2 0.9±0.2* Glycine, % 0.8±0.3 0.9±0.3 Lysine, % 1.5±0.5 1.8±0.5 Aspartic acid,% 2.0±0.8 2.5±0.8 Glutamic acid,% 2.8±1.3 3.3±1.2 Mass fraction of leucine and isoleucine (total) 2.0±0.5 2.4±0.5 Methionine, % 0.4±0.2 0.7±0.2 Proline, % 0.5±0.2 0.7±0.2 Serine, % 0.4±0.2 0.9±0.2* Tyrosine, % 0.6±0.2 0.8±0.2 Threonine, % 0.6±0.3 1.1±0.3 Phenylalanine, % 0.7±0.3 0.9±0.3 Cystine, % 0.3±0.1 0.7±0.1* Total amino acids: 17.4 22.7 *p < 0.05

According to Table 2, it was found that the complex introduction of feed additives “Abiopeptide” and “Ferropeptide” had a positive effect on the amino acid composition of rabbit meat. Thus, the amount of amino acids in the experimental group is 5.3% higher compared to the control. Including a significant increase in the content of alanine (by 0.6%), histidine (by 0.4%), serine (by 0.5%) and cystine (by 0.4%) in meat.

Analysis of the results of the experiments led to the conclusion that oral administration of “Abiopeptide” with food to Soviet chinchilla rabbits at a dose of 1 ml/kg of live body weight daily from 10 days to 60 days of age, as well as two-time administration with water for watering "Ferropeptide" at a dose of 0.5 ml/kg of animal weight daily for 2-3 months helped to increase the content of a number of amino acids in rabbit meat, as well as to prevent the development of microelementosis in them.

Literature

1. The influence of a new complex feed additive on the biological characteristics of rabbits / V. M. Bachinskaya, A. A. Deltsov, D. V. Gonchar, A. T. Mysik // Zootechniya. – 2023. – No. 12. – P. 19-22.

2. Parasyuk, L. P. Study of the stability of a multicomponent preparation based on vitamins, amino acids and microelements “Abiotonic” during storage / L. P. Parasyuk, A. A. Deltsov, V. M. Bachinskaya // Current problems of veterinary medicine, zootechnics , biotechnology and examination of raw materials and products of animal origin: Collection of proceedings of the scientific and practical conference, Moscow, November 08, 2022 / Under the general editorship of S.V. Pozyabina, L.A. Gnezdilova. – Moscow: Agricultural Technologies, 2022. – P. 285-286.

3. Mikulets, Yu. I. Compatibility of vitamins and bioelements in feeding rabbits / Yu. I. Mikulets, K. V. Kharlamov // Veterinary and feeding. – 2019. – No. 1. – P. 40-43.

4. Radzhabov A.I., Iskandarova Sh.I., Nomozova D.I. The influence of macro- and microelements on some physiological parameters of rabbits // Bulletin of Science. 2020. No. 9 (30).

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Claims (1)

A method for improving the amino acid composition of rabbit meat while simultaneously preventing oxidative stress in them, including oral administration of the drug Abiopeptide with food for rabbits at a dose of 1 ml/kg of live body weight daily from 10 days to 60 days of age and twice the administration of the drug Ferropeptide with water for drinking. at a dose of 0.5 ml/kg animal weight daily for 2-3 months.