RU2846783C1 - Feedstuff for laboratory animals, reducing hemic hypoxia - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention relates to veterinary science and physiology, in particular, to feedstuff with liquorice root extract for laboratory animals. Feedstuff for laboratory animals contains: fish flour, wheat flour, premix, sunflower oil, sunflower cake, saline, corn, oat flour, dry skim milk, bulgur groats, rice, liquorice root extract at certain ratio of initial components in wt. %.

EFFECT: invention usage will allow to enhance the quality of feedstuff for laboratory animals.

1 cl, 2 tbl

Description

The invention relates to veterinary science and physiology, in particular to compound feed with licorice root extract based on the naked licorice plant ( Glycyrrhiza glabra L.) for laboratory animals - non-linear white rats, which improves the oxygen transport function of the blood.

A compound feed is known (RU Patent No. 2769986, 2022), which includes fish meal, yeast, sunflower cake, soybean meal, wheat flour, premix, sunflower oil, vitamin B-4, crustacean meal, pumpkin flour, glasswort, with the following ratio of the original components, wt. %:

Fish meal 15.00 Yeast 5.00 Sunflower cake 25.00 Soybean meal 15.00 Wheat flour 10.00 Premix 0.20 Sunflower oil 5.00 Vitamin B-4 0.10 Crustacean flour 7.50 Pumpkin flour 17:00 Salicornia 0.20

The disadvantage of this feed is the absence of a component that improves the saturation of red blood cells with hemoglobin, which is necessary for the oxygen-transport function of the blood for laboratory animals.

The closest analogue (prototype), similar in purpose, is a production compound feed for laboratory animals, which has a hypotensive effect (see patent RU2801534, 2023) containing fish meal, wheat flour, premix, sunflower oil, sunflower cake, glasswort, additionally contains corn, oatmeal, dry skim milk, bulgur cereal, rice, with the following ratio of the original components, wt. %:

Fish meal 20.0 Wheat flour 25.0 Corn 10.0 Oat flour 10.0 Dry skim milk 5.0 Sunflower cake 10.0 Bulgur groats 6.5 Rice 11.0 Premix 1.5 Sunflower oil 0.7 Salicornia 0.3

A disadvantage of the well-known compound feed for laboratory animals is the absence of a component that would, under conditions of increased hemodynamics, reduce the degree of imbalance between the body's increased needs for effective blood supply and the capabilities of the heart and, as a result, eliminate disruptions in the functioning of the cardiovascular system of animals during rapid growth, as well as the occurrence of a number of various physiological deviations in the oxygen-transport function of the blood.

The technical result is an increase in the quality of compound feed for laboratory animals by introducing an extract of licorice root based on the plant naked licorice ( Glycyrrhiza glabra L.) into the diet of laboratory nonlinear white rats to improve the oxygen transport function of the blood.

The technical result is achieved in that the known compound feed containing fish meal, wheat flour, premix, sunflower oil, sunflower cake, glasswort, corn, oat flour, dry skim milk, bulgur cereal, rice, additionally contains licorice root extract in the following ratio of the original components, wt. %:

Fish meal 20.0 Wheat flour 24.0 Corn 10.0 Oat flour 10.0 Dry skim milk 5.0 Sunflower cake 10.0 Bulgur groats 6.5 Rice 11.0 Premium c1.5 Sunflower oil 0.7 Salicornia 0.3 Licorice extract root 1.0

It has been established that the extract of licorice root ( Glycyrrhiza glabra L.) was used as a phytomedicine with hemoglobinemic action and protective action against hemic hypoxia.

The novelty of the proposed invention is that it utilizes licorice root extract ( Glycyrrhiza glabra L.) at a mass fraction of 0.1% as a phytomedicine. The application of a dose of 1 mg per 1 kg of compound feed is consistent with our own accumulated experimental data.

According to the literature, licorice root contains a number of biologically active substances, such as triterpene saponins, which have a hypocholesterolemic effect, i.e., they lower cholesterol levels and prevent the formation of atherosclerotic plaques. Considering that it is the accumulation of such plaques in the coronary arteries that provokes chronic hemodynamic hypoxia and is the main cause of coronary heart disease, licorice can be considered as a prophylactic plant that prevents the development of this disease. Other biologically active substances contained in licorice root are flavonoids, coumarins, and phenols (see the article by Koshkina A.V., Fedotova Yu.O. Licorice. Phytochemical composition and biological effects // Orbital. 2018. No. 2 (3). pp. 30-51).

The qualitative composition of naked licorice root is represented by 16 amino acids. Of these, 7 are essential (monoaminomonocarboxylic: threonine, valine, methionine, isoleucine, leucine, phenylalanine; diaminomonocarboxylic: lysine) and 9 are replaceable (monoaminomonocarboxylic: alanine, glycine, tyrosine, serine; monoaminodicarboxylic: aspartic and glutamic acids; diaminomonocarboxylic: arginine; heterocyclic: histidine and proline) (see the article by Nedilko O.V., Yanitskaya A.V. Study of the amino acid composition of the aboveground and underground parts of naked licorice // Chemistry of plant raw materials. 2020. No. 1. pp. 251-256).

Licorice root extract is a hot-water extraction of the root followed by evaporation and concentration of the juice in vacuum apparatuses. The dry matter content is 62-68% (see GOST 22840-77, Licorice Root Extract). Table 1 shows the characteristics of licorice root extract.

The proposed compound feed was produced using a known wet-pressing method. The components, according to the proposed recipe, were thoroughly mixed to obtain a homogeneous mass (see the book by Ponomarev SV et al., "Technologies for Growing and Feeding Aquaculture Objects in Southern Russia." - Astrakhan: Nova Plus, 2002. - pp. 212-222). The components in the specified percentage ratio preserved the nutritional value of the compound feed without disturbing the protein balance, in accordance with GOST 34566-2019. Interstate standard. Complete compound feed for laboratory animals. Specifications.

Sexually mature males (6-8 months, n=30) with an average weight of 190.0 g were taken for the experiment. First, nonlinear white rats were selected into salt-sensitive and salt-resistant ones, for which purpose table salt was administered per os at a dose of 1 g/kg of the animal’s body weight for 14 days. If during this period the pressure rose and was maintained by 10%, then these rats were considered salt-sensitive (M.V. Galkina, L.B. Snopova, N.N. Prodanets, R.D. Lapshin, I.I. Belousova, D.A. Abrosimov, M.L. Bugrova. Atrial and brain natriuretic peptides of secretory cardiomyocytes under conditions of salt load in an experiment. Modern technologies in medicine. 2016. Vol. 8, No. 3, p. 51). Two groups of salt-sensitive rats were formed. The first group included 10 control rats fed a standard diet (0.3% NaCl) for 2 months (control). The second group (10 rats) were fed the prototype diet, and the third contained licorice root extract at a concentration of 0.1% per 1 kg of feed.

The diets were identical in protein, fat, and carbohydrate content. Water was available ad libitum. Statistical analysis was performed using the Excel statistical package and Statistica software, v. 12.0, taking into account normal distribution (Kolmogorov-Smirnov test). The critical level of statistical significance for paired groups was p < 0.05.

Table 2 shows the physiological parameters of white nonlinear rats when kept on the proposed compound feed, the prototype feed, and the feed for the control group.

Table 2 shows an improvement in the physiological condition of animals on the proposed compound feed.

Compared to the prototype, the results of the study showed that hemoglobin levels in the group of animals fed a diet containing licorice root extract increased to control group levels. In the group fed the prototype feed, hemoglobin levels were 12.3% lower than in the group fed the proposed compound feed (the differences are statistically significant at p < 0.05).

Systolic pressure in the group using the prototype feed and in the group on the proposed compound feed were in comparable ranges of 91.02±3.06 mmHg and 91.03±3.04 mmHg, which amounted to a decrease of 18% compared to the control. Diastolic pressure in the group using the prototype feed and the proposed compound feed was in comparable ranges of 67.9±93.01 mmHg and 68.96±3.03 mmHg, which amounted to a decrease of 21% and 19.9% compared to the control. Heart rate in the group on the prototype feed and the proposed compound feed was lower by 16% and 15%, respectively, compared to the control.

Positive effect: the proposed compound feed prevents the development of hemic hypoxia by optimizing the oxygen transport function of the blood in laboratory animals.

Claims (2)

Compound feed for laboratory animals that reduces hemic hypoxia, including fish meal, wheat flour, corn, oat flour, dry skim milk, sunflower cake, bulgar groats, rice, premix, sunflower oil, glasswort, licorice root extract in the following ratio of the original components, wt.%: Fish meal 20.0 Wheat flour 24.0 Corn 10.0 Oat flour 10.0 Dry skim milk 5.0 Sunflower cake 10.0 Bulgur groats 6.5 Rice 11.0 Premix 1.5 Sunflower oil 0.7 Salicornia 0.3 Licorice extract root 1.0