RU2841770C1 - Method for production of synbiotic fodder additive for pigs - Google Patents

Abstract

FIELD: agriculture; biotechnology.

SUBSTANCE: method for production of synbiotic fodder additive for swine relates to biotechnology and agriculture. In a method comprising obtaining a biomass of lyophilized lactic acid bacteria based on lactic acid microorganisms and mixing them with a vegetable filler consisting of powdered maltodextrin and edible citrus fibre, according to invention microorganisms used are Lactobacillus amylovorus VKM B-3750D in amount of 0.5-1.0 %, wherein vegetal origin filler is added with powdered maltodextrin in amount of 75-84.5 % and food citrus fibre in amount of 10-15 %, in addition, apple pectin is further added in amount of 5-10 % of the resulting mass of the synbiotic feed additive to achieve the titre of the culture in the additive of at least 10⁹ cells/g.

EFFECT: increase of safety and meat productivity of pigs.

1 cl, 5 tbl, 3 ex

Description

The invention relates to biotechnology and agriculture, and specifically to a method for producing a synbiotic feed additive based on lactic acid bacteria and a filler of plant origin for feeding pigs in order to increase their survival and meat productivity.

In industrial pig production, early weaned piglets face social, environmental and dietary stresses caused by handling, transport, litter mixing, separation from the mother and the transition from a milk diet to solid feed. Dietary changes at weaning are associated with low feed and water intake, gastrointestinal changes and modifications of the piglet gut microbiota. Microbial communities inhabiting the gastrointestinal tract perform many functions, such as participating in the digestion of organic compounds and fermentation of carbohydrates to make metabolites available to the host, contributing to host defense against pathogenic bacteria, and any changes in the microbial background have already been associated with numerous diseases or infections. The transition to weaning is associated with diarrhea and enteric diseases, which are the main causes of mortality in piglets and may be a direct consequence of the microbial shifts observed during this critical period. In addition to increasing the economic burden on the pig industry, weaning-associated infections are a public health concern due to the widespread use of antimicrobials for therapeutic purposes.

In this regard, the question arises of using safe feed additives and preparations of microbial origin that allow normalizing the bacterial balance towards beneficial microflora in order to reduce the impact of unfavorable stress factors on the body of young pigs. Thus, the search for highly effective microbial compositions based on pigs' own (autochthonous) microflora is a relevant and promising direction.

There are ways to increase the meat productivity of pigs by introducing microelements, enzymes, vitamins, and probiotics into their diets. At the same time, beneficial microorganisms that exhibit probiotic properties, but are not the natural microflora of the gastrointestinal tract of pigs, are often used in the composition of microbial feed additives.

A method is known that involves adding pectin to the nutrient medium at a rate of 5-6 g/l (0.5-0.6%), which ensures the accumulation of microorganism biomass and imparts prebiotic properties to the fermented milk product (Patent of the Russian Federation No. 2023396, IPC A23C 9/12, A23C 9/127, 02.03.1993).

A known method involves the use of a probiotic supplement in pig diets, including three types of lactic acid bacteria: Lactobacillus curvatus , Lactobacillus intermedius and Lactobacillus salivarius , grown on a molasses-ammonium medium: ammonium chloride - 0.9 g / l; beet molasses - 15.0 g / l; corn extract - 10.0 ml / l; dry milk whey - 50.0 g / l (Shkredov V.V. Improving the productive qualities of piglets during the growing period using the new probiotic supplement Gallobact-F: diss. for co-authorship. student. degree. candidate of biological sciences / V.V. Shkredov. - Ekaterinburg, 2022. - 153 p.).

The disadvantage of this method is that the probiotic contains microorganisms that are not natural representatives of the microbiome of the gastrointestinal tract of this type of animal, and it is also presented in liquid form, which technologically complicates the process of introducing it into the diet of animals, requires the use of additional equipment (dispensers), which in combination will not ensure the maximum realization of the biological potential of pigs.

A method for producing a probiotic supplement is known, which includes cultivating Lactobacillus parabuchneri B-13061 in a medium consisting of feed molasses 45.0 g, K ₂ HPO ₄ - 2.0 g and yeast extract - 0.02 g per 1 liter of water, and powdered apple pectin is added to the nutrient medium (RU Patent No. 2759703, IPC A23K 50/70, A23K 10/10, SPC A23K 50/70, A23K 10/10, 11/17/2021).

The closest method is the production of a synbiotic additive for agricultural animals and poultry, which includes a dry culture of lactic acid bacteria: Bifidobacterium lactis 2.5-3.0%, Lactobacillus acidophilus 2.5-3.0%, Streptococcus thermophiles 2.5-3.0%, Lactobacillus delbrueckii ssp. Bulgaricus 2.5-3.0%, and fillers of plant origin: dietary citrus fiber 14.0-15.0%, maltodextrin 76.0-84.0% (Boyko A.A. Efficiency of using the probiotic feed additive "SBT-Lacto" in growing broiler chickens: diss. for co-author's degree of candidate of biological sciences / A.A. Boyko. - Yekaterinburg, 2023. - 153 p.).

The disadvantage of the known technical solution is that the synbiotic contains microorganisms that are not natural representatives of the microbiocenosis of the gastrointestinal tract of animals and poultry, as well as insufficient diversity of the prebiotic substance for the filler during its manufacture, as a result of which, when using this feed additive in the diet of animals and poultry, the maximum survival and growth of beneficial microorganisms in the gastrointestinal tract will not occur.

The technical result is an increase in the preservation and indicators of meat productivity of pigs, due to ensuring the viability and growth of lactic acid cultures in the gastrointestinal tract of pigs, which are representatives of the natural microbiocenosis of the gastrointestinal tract of pigs, due to the use in the composition of the synbiotic, in addition to powdered maltodextrin and food citrus fiber, apple pectin is additionally introduced.

The technical result is achieved in that in the method for producing a synbiotic feed additive for pigs, which includes obtaining a biomass of lyophilized lactobacilli based on lactic acid microorganisms and mixing them with a filler of plant origin consisting of powdered maltodextrin and food citrus fiber, according to the invention, Lactobacillus amylovorus VKM B-3750D is used as microorganisms in an amount of 0.5-1.0%, while apple pectin is additionally introduced into the composition of the filler of plant origin in an amount of 5-10% of the obtained mass of the synbiotic feed additive.

The novelty of the claimed technical solution is due to the fact that the synbiotic feed additive contains lactic acid microorganisms Lactobacillus amylovorus VKM B-3750D, which are natural representatives of the microbiocenosis of the cecum of pigs (Study of the probiotic properties of lactobacilli isolated from the gastrointestinal tract of pigs / Yu.A. Lysenko, A.V. Luneva, A.Kh. Shantyz [et al.] // Veterinary Science and Feeding. - 2023. - No. 4. - P. 58-61. - DOI 10.30917 / ATT-VK-1814-9588-2023-4-17) and a filler of plant origin consisting of powdered maltodextrin in an amount of 75-85%, dietary citrus fiber in an amount of 10-15% and additionally apple pectin is introduced in an amount of 5-10% of the weight of the synbiotic additive to maintain the viability and growth of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs.

The features that distinguish the claimed technical solution from the prototype are aimed at achieving a technical result and have not been identified during the study of this and related fields of science and technology and, therefore, meet the criterion of “inventive step”.

These differences allow us to conclude that the declared technical solutions meet the “novelty” criterion.

The compliance of the claimed solution with the patentability criterion “industrial applicability” is due to the fact that the proposed technical solution is operational and can be used for feeding agricultural animals, in particular pigs.

The method of producing a synbiotic feed additive for pigs is as follows.

The cultivation of Lactobacillus amylovorus VKM B-3750D microorganisms is carried out in a medium consisting of feed molasses - 45.0 g, K ₂ HPO ₄ - 2.0 g, yeast extract - 0.02 g. Lactobacillus amylovorus VKM B-3750D microorganisms with a titer of at least 1.0×10 ⁵ CFU/ml in an amount of 100.0 g/l are added to the prepared nutrient medium and cultivated at a temperature of 37°C for 24 hours.

When using the seed culture Lactobacillus amylovorus VKM B-3750D in an amount of less than 100.0 g/l, the required culture titer will not be achieved.

When using the seed culture Lactobacillus amylovorus VKM B-3750D in an amount of more than 100.0 g/l, the same titer is achieved and there is no point in taking a larger amount.

After completion of cultivation in the fermenter, Lactobacillus amylovorus VKM B-3750D is concentrated using a flow centrifuge with a cylindrical rotor.

As the rotor fills, the centrifuge is stopped and the rotor is cleaned. The bacterial mass is separated by washing the supernatant from the rotor and subsequent resuspension until the cell titer reaches at least 10 ¹² cells/ml.

Lyophilization of the bacterial concentrate Lactobacillus amylovorus VKM B-3750D is carried out in sublimation drying by pouring into cassettes, after mixing with a cryoprotectant.

Obtaining the finished form of the synbiotic feed additive includes aseptic mixing of the lyophilized bacterial concentrate with a filler of plant origin. The amount of bacterial concentrate Lactobacillus amylovorus VKM B-3750D in the composition of the synbiotic is 0.5-1.0%, powdered maltodextrin in the amount of 75-85%, food citrus fiber in the amount of 10-15% and apple pectin in the amount of 5-10% of the weight of the synbiotic additive, which ensures the achievement of a culture titer in the additive equal to at least 10 ⁹ cells/g.

When using the bacterial concentrate Lactobacillus amylovorus VKM B-3750D in an amount of less than 0.5% of the mass of the synbiotic supplement, the required titer of the culture in the supplement will not be achieved.

When using the bacterial concentrate Lactobacillus amylovorus VKM B-3750D in an amount of more than 1.0% of the mass of the synbiotic supplement, the required titer of the culture in the supplement is achieved and there is no point in taking a larger amount.

The simultaneous use of three components as plant-based fillers (powdered maltodextrin, dietary citrus fiber, apple pectin) in a synbiotic supplement will ensure maximum support for the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs.

When using fillers of plant origin alone or in any other combination, it will not provide maximum support for the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs.

When using maltodextrin as a filler component in an amount of less than 75% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will not be maintained.

When using maltodextrin as a filler component in an amount greater than 85% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will be maintained and there is no point in taking a larger amount, as this makes the product more expensive.

When using dietary citrus fiber as a filler component in an amount of less than 10% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will not be maintained.

When using dietary citrus fiber as a filler component in an amount of more than 15% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will be maintained, and there is no point in taking a larger amount, as this makes the product more expensive.

When using powdered apple pectin as a filler component in an amount of less than 5% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will not be maintained.

When using powdered apple pectin as a filler component in an amount of more than 10% of the synbiotic additive weight, the growth and viability of Lactobacillus amylovorus VKM B-3750D in the gastrointestinal tract of pigs will be maintained and there is no point in taking a larger amount, as this makes the product more expensive.

The synbiotic feed additive developed by the proposed method can be used in pig breeding to increase the survival rate and meat productivity of animals.

A specific implementation of the method is presented in Example 1, and the effectiveness of using the synbiotic feed additive obtained according to the invention on pigs is demonstrated in Examples 2 and 3.

Example 1. For the production of a synbiotic feed additive for pigs, microorganisms Lactobacillus amylovorus VKM B-3750D and a filler of plant origin consisting of powdered maltodextrin, dietary citrus fiber and apple pectin are used.

The technological process for producing a synbiotic additive includes the following stages:

- obtaining pure cultures of Lactobacillus amylovorus VKM B-3750D;

- storage of pure cultures of Lactobacillus amylovorus VKM B-3750D;

- propagation of pure cultures of Lactobacillus amylovorus VKM B-3750D, in skim milk for 1-3 days at a temperature of 37°C in test tubes under laboratory conditions;

- preparation of mother culture (culture liquid) in flasks and three-liter jars based on Lactobacillus amylovorus VKM B-3750D;

- cultivation of Lactobacillus amylovorus VKM B-3750D in a fermenter;

- concentration of Lactobacillus amylovorus VKM B-3750D by centrifugation;

- mixing the bacterial mass Lactobacillus amylovorus VKM B-3750D with a cryoprotector;

- lyophilization (freeze-drying) of probiotic strains Lactobacillus amylovorus VKM B-3750D;

- mixing of lyophilisate of Lactobacillus amylovorus VKM B-3750D with a filler of plant origin;

- packaging of synbiotic feed additives;

- assessment of the quality of the dietary supplement;

- storage of finished products.

Lactobacillus cultures - Lactobacillus amylovorus VKM B-3750D - are non-pathogenic, are saprophytes, demanding of sterile conditions, by morphology are short rods with a size of 3-4 microns and a thickness of up to 0.5 microns.

Pure culture of Lactobacillus amylovorus VKM B-3750D is inoculated into test tubes with sterile skim milk (skim milk). Cultivated for 72 hours at 38°C.

The nutrient medium for the stock culture of the first generation _of bacteria is prepared on a glucose-peptone medium: _Na2HPO412 - _aqueous - 3.2 g/l; _KH2PO4 - 0.3 g/l; _MgSO4 - 0.5 g/l; NaCl - 0.5 g/l; peptone - 2.0 g/l; yeast extract - 0.05 g/l; glucose - 25.0 g/l.

The mother culture of the second generation of bacteria in three-liter jars is also cultivated on a glucose-peptone medium. The hydrogen index is 6.8-7.2. Sterilization of the GPS is 1 atm for 40 min. Glucose is sterilized separately from the nutrient medium at 0.5 atm for 20 min, followed by addition to the sterilized medium. Glucose is added directly to the flasks during the seeding of the mother culture.

The growth of the mother culture of the first and second generations lasts 5-7 days at a temperature of 37°C. After this, the purity of the cultures is checked. If the quality of the mother culture is good, it is sent for seeding into the fermenter.

Nutrient medium for growing Lactobacillus amylovorus VKM B-3750D in industrial conditions consists of feed molasses - 45.0 g, K ₂ HPO ₄ - 2.0 g, yeast extract - 0.02 g per 1 liter of water. The components of the nutrient medium are mixed with distilled water and sterilized. Microorganisms Lactobacillus amylovorus VKM B-3750D with a titer of at least 1.0×10 ⁵ CFU/ml in an amount of 100.0 g/l (10% of the nutrient medium weight) are added to the resulting nutrient medium and cultivated at a temperature of 37°C for 24 hours.

Mode of growing culture in a fermenter:

- cultivation temperature - 37°C;

- aeration - absent;

- stirrer speed - 5-10 rpm;

- pH - 6.8-7.2;

- titration - 5% KOH;

- foam suppression - adecanol;

- cultivation time - 24 hours;

- achieved cell titer - not less than 10 ⁹ cells/ml.

During fermentation, samples are periodically taken to monitor the ongoing process: the hydrogen index (pH) of the biopreparation, the purity of the cultivated strain, the presence of foreign microflora. At the end of the process, samples are taken to determine the titer, which for lactobacilli in the liquid form of the biopreparation should be at least 1.0 × 10 ⁹ active bacterial cells in 1 cm ³ .

The fermenter must be equipped with the necessary equipment to perform the following technological operations: cultivation of lactic acid microorganism strains; mixing; pH-stating; recording the pH value in continuous mode; sowing through an inoculation container using a peristaltic pump.

Sterilization is carried out according to the following regime: heating and holding at a temperature of 121±1°C for 20 min to remove air from the fermenter; heating to a temperature of 121±1°C (1.0 atm) with holding at this temperature for 30 min and steaming all branches; cooling the nutrient medium to a temperature of 37°C, maintaining excess pressure with sterile air in the fermenter cavity of 0.04±0.01 MPa.

At the time of sterilization of the nutrient medium for strains of lactic acid microorganisms, the inoculation tank with the mother culture and the tank with the 25% aqueous ammonia solution must be installed and connected to the fermenter.

After the nutrient medium reaches a temperature of 37°C, the pH value must be set at 7.0. If necessary, the microbiologist informs the operator and aseptically performs a control sample collection for chemical analysis and sterility control.

Inoculation is carried out by aseptically transferring the liquid mother culture from the inoculation tank to the fermenter using a peristaltic pump. At the moment of transfer, excess pressure of 0.01-0.02 MPa should be maintained in the fermenter cavity.

After 10-12 and 20 hours of cultivation, a sample is taken through a sampler that has been pre-steamed for 15 minutes.

Based on the analysis of the control sample, a conclusion is made about the completion of the cultivation process.

After completion of cultivation in the fermenter, the bacterial culture Lactobacillus amylovorus VKM B-3750D is concentrated using a flow centrifuge with a cylindrical rotor. Centrifugation parameters:

- centrifugation temperature - 25-35°C;

- rotor speed - 8,000 rpm.

As the rotor fills, the centrifuge is stopped and the rotor is cleaned. The bacterial mass is separated by washing the supernatant from the rotor and then resuspending it in a ratio with the liquid phase of no more than 1:1. The achieved cell titer is no less than 10 ¹² cells/ml. The bacterial concentrate is stored for no more than 24 hours at a temperature of +2-4°C.

Lyophilization of the bacterial concentrate is carried out in sublimation drying by pouring into cassettes, after mixing with a cryoprotector in a ratio of 1:1, according to the manufacturer's requirements. Storage of the lyophilized bacterial concentrate is carried out in a refrigerator at a temperature of +4-6°C.

The production of the finished form of the synbiotic feed additive includes aseptic stepwise mixing of the lyophilized bacterial concentrate with a filler of plant origin. The amount of bacterial concentrate Lactobacillus amylovorus VKM B-3750D in the composition of the synbiotic is 0.5-1.0%, powdered maltodextrin in the amount of 75-85%, food citrus fiber in the amount of 10-15% and apple pectin in the amount of 5-10% of the weight of the synbiotic additive, until the culture titer in the additive is achieved, equal to at least 10 ⁹ cells/g.

The synbiotic feed additive is packaged in multilayer paper bags with a polyethylene liner with a capacity of 0.5; 1.0; 2.0; 25.0 kg and stored in a clean, dark room at a temperature of +2 to 10°C.

The quality indicators of the synbiotic feed additive are presented in Table 1. The quality of the finished synbiotic feed additive is assessed based on organoleptic, physicochemical and microbiological characteristics.

The finished form of the synbiotic feed additive is represented by viable strains of Lactobacillus amylovorus VKM B-3750D in a titer of at least 1.0 × 10 ⁹ CFU/g and is a powder, from white to beige in color, odorless, with a moisture content of no more than 10.0%.

Example 2. To increase pig productivity, a synbiotic feed additive consisting of lyophilized bacterial mass Lactobacillus amylovorus VKM B-3750D and a filler of plant origin (powdered maltodextrin, food citrus fiber and apple pectin) was used. To select the optimal dose for using the synbiotic additive, scientific experiments were conducted on suckling piglets.

The studies were conducted at the Pyatachok training and production complex (Krasnodar). Four groups of suckling piglets were selected based on the analog group principle: Group 1 (control) was kept in the same conditions as the other (experimental) groups and received the standard feeding ration adopted at the enterprise (SR); Group 2 received the same ration with the addition of 0.5% synbiotic feed additive (SR+KD 0.5%); Group 3, similar to Group 2, received the synbiotic feed additive at a dose of 0.7% of the feed weight (SR+KD 0.7%); Group 4, under the same conditions as the other groups, received the synbiotic at a dose of 1.0% (SR+KD 1.0%). During the experiment, all piglets had unlimited access to drinking water from nipple drinkers. The scheme of the experiment for selecting the optimal dose of synbiotic feed additive for introduction into the diet of suckling piglets is presented in Table 2.

The productivity, safety and conversion rates of the feed of suckling piglets in the experimental and control groups when a synbiotic feed additive was introduced into the diet are presented in Table 3.

During the observation of piglets of the 3rd and 4th experimental groups, the clinical picture and the situation in the places of stay of animals were characterized, during the entire stage of the experiment, by a consistently positive clinical status, namely, the body temperature of animals corresponded to the norms for this age group of animals (on average, 39.5 ° C), the piglets had a strong constitution, good nutrition (rounded contour lines of the body) and a tender-dense constitution, a lively temperament expressed in active behavior. In the places of stay of animals, no signs of intestinal disorders were observed, feces were well formed, without mucus, had a color characteristic of the type of animals. There was no mortality of animals. During the monitoring of the condition of piglets after the planned technological operations - tail docking, castration of boars and the use of iron-containing drugs, clinical manifestations of gastrointestinal disorders were not registered.

The control and 2nd experimental groups had similar health indicators from the 3rd and 4th to 5-8 days after birth. After the planned technological operations - tail docking, castration of boars and the use of iron-containing drugs, the clinical picture and the situation in the places of stay of the animals were characterized, during the entire stage of the experiment, by an imbalance in the general condition of the animals of the examined groups. It was expressed in the periodic manifestation of signs of diarrhea in individual animals, the consequence of which was a decrease in activity in the groups, the presence of individuals with signs of intoxication and contamination of the places of stay of piglets with excrement of watery consistency. The mortality of animals from among the control animals was 3.33% (2 heads), in the 2nd experimental group - 1.66% (1 head), while in the 3rd and 4th experimental groups all animals survived.

The results of Table 3 show the prevalence of the final weight of piglets in the experimental groups and their average gain in comparison with the control group. The first indicator in the experimental groups statistically reliably (at p≤0.05) exceeded the control by 5.76% (470 g), 11.29% (920 g) and 14.84% (1210 g), respectively, in the 2nd, 3rd and 4th experimental groups. The average gain in live weight of a piglet in the 2nd, 3rd and 4th experimental groups exceeded the indicators of the control animals by 6.66% (470 g); 12.90% (910 g) and 17.44% (1230 g), respectively. The cost of compound feed per unit of production in the 2nd experimental group corresponded to the indicators of the control group, the data obtained for the 3rd experimental group reflect a decrease in the indicator by 3.07% and by 4.61% in the 4th experimental group of piglets.

In general, during the technological period of growing suckling piglets, the maximum survival rate (100%) was in the 3rd and 4th experimental groups, while in the control and 2nd experimental groups, taking into account mortality and culling, it was 95.0 and 96.6%.

Technological operations during this period of growth of young pigs allow obtaining the required economic indicators, but are the cause of stress for animals and at the same time have a negative impact on their body, which was revealed to a greater extent in the control, as well as in the 2nd experimental groups.

Example 3. To increase pig productivity, a synbiotic feed additive consisting of lyophilized bacterial mass Lactobacillus amylovorus VKM B-3750D and a filler of plant origin (powdered maltodextrin, food citrus fiber and apple pectin) was used. To select the optimal dose for using the synbiotic additive, scientific experiments were conducted on piglets in the growing group.

The studies were conducted at the Pyatachok training and production complex (Krasnodar). Four groups of rearing piglets were selected based on the analog group principle: Group 1 (control) was kept in the same conditions as the other (experimental) groups and received the standard feeding ration adopted at the enterprise (SR); Group 2 received the same ration with the addition of 0.5% synbiotic feed additive (SR+KD 0.5%); Group 3, similar to Group 2, received the synbiotic feed additive at a dose of 0.7% of the feed weight (SR+KD 0.7%); Group 4, under the same conditions as the other groups, received the synbiotic at a dose of 1.0% (SR+KD 1.0%). During the experiment, all piglets had unlimited access to drinking water from nipple drinkers. The scheme of the experiment for selecting the optimal dose of synbiotic feed additive for introduction into the diet of piglets of the rearing group is presented in Table 4.

During the first 10-14 days of the rearing period, clinical examination of piglets revealed unstable feed consumption in all experimental groups, which was largely influenced by the increased excitability of animals in a new place, manifested by synchronous running of groups of animals within the section and an acute reaction to sound stimuli. During the acclimatization period of piglets in the control group, signs of defecation were recorded in the rest and feeding areas, which is the reason for the change in habitat and diet, while in the experimental groups these stress phenomena were expressed to a lesser extent. Body temperature, heart rate and respiratory movements in all groups were slightly increased during the transition period, which gradually returned to normal.

The results of productivity, safety and conversion of feed for piglets in the growing experimental and control groups are presented in Table 5.

As a result of 56 days of growing experimental piglets at the growing stage, the 2nd and 3rd experimental groups of animals exceeded the weight of animals at the end of the control group stage by 2.30% and 10.18%. The 4th experimental group reliably exceeded the analyzed indicator compared to the control by 16.31% (p≤0.05). The average increase in the weight gain of one head (gain over the technological period) of the 2nd, 3rd and 4th groups corresponds to the indicators of 1.36%, 9.60% and 17.31% relative to the control data. The feed conversion indicators of growing piglets reflect a decrease in their costs per 1 kg of gain in the experimental groups by 0.43, 6.55, and 10.48%, respectively, in the 2nd, 3rd and 4th experimental groups relative to the control.

The survival rate of the livestock in the period under review was 92% in the control group with an equal ratio of mortality and culling of 4% (2 heads each). The yield of commercially valuable livestock in the 2nd experimental group was 96% with equal proportions of mortality and culling - 2% (1 head each), which exceeded the control indicator by 4%. In the 3rd experimental group, there was 98% survival and 2% (1 head) of culling. There was no loss of livestock in the 4th experimental group throughout the experiment, the survival rate was 100%.

Thus, the use of the studied synbiotic feed additive in young pigs at an early age contributed to a decrease in the negative consequences of mandatory planned technological operations carried out on the farm; an increase in survival and productivity indicators was observed due to higher gains, while simultaneously reducing feed costs per unit of production, which was especially pronounced in the group where 1.0% of the synbiotic feed additive from the feed weight was used (4th experimental group).

In general, the increased dynamics of live weight of young pigs in the experimental groups can be explained by the positive effect of the synbiotic feed additive consisting of lyophilized bacterial mass of Lactobacillus amylovorus VKM B-3750D and a filler of plant origin (powdered maltodextrin, food citrus fiber and apple pectin), which contribute to better absorption of energy and nutrients of the compound feed by pigs, which affected their meat productivity (increased rates of live weight gain).

Claims (1)

A method for producing a synbiotic feed additive for pigs, comprising obtaining a biomass of lyophilized lactobacilli based on lactic acid microorganisms and mixing them with a filler of plant origin consisting of powdered maltodextrin and food citrus fiber, characterized in that Lactobacillus amylovorus BKM B-3750D is used as microorganisms in an amount of 0.5-1.0%, while powdered maltodextrin in an amount of 75-84.5% and food citrus fiber in an amount of 10-15% are introduced into the composition of the filler of plant origin, in addition, apple pectin is additionally introduced in an amount of 5-10% of the obtained mass of the synbiotic feed additive until the titer of the culture in the additive is achieved, equal to at least 10 ⁹ cells/g.