RU2764138C1 - Method for increasing the fertilisation rate of mares - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention constitutes a method for increasing the fertilisation rate of mares, consisting in the use of a medicinal product based on an alcoholic extract of reindeer antlers and arabinogalactan, administered to mares five days prior to the intended mating, three times, at an interval of one day, at a dose of 5 ml.

EFFECT: developed method for increasing the fertilisation rate of mares using a medicinal product of natural origin provides a possibility of increasing the fertilisation rate of mares and yield of foals, continuing the use of horses upon completion of the sports career thereof.

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Description

The invention relates to veterinary medicine, in particular to the field of reproduction, in particular the regulation of the reproductive function of horses. The method can also be used to increase the reproduction of other types of productive animals.

Increasing the yield and safety of foals remains an urgent task for horse breeding, especially for large stud farms. The problem consists of a low pregnancy rate in mares, often low resistance and frequent morbidity in newborn foals, who sometimes have several infections before weaning. This inevitably affects their further development and subsequent use.

In modern conditions of the development of horse breeding, the methods of stimulating the reproductive function in animals are subject to requirements that ensure high efficiency, safety and availability. These requirements are met by physical methods, both traditional (massage, thermal exposure, exercise, acupuncture, etc.), and new hardware ones (electric currents of various characteristics, laser radiation, ultrasound, vibration massage, electroacupuncture of biologically active points, etc.).

Known methods of stimulating the reproductive function of cows after calving, based on the use of hormonal drugs: analogues of releasing hormone (Gn-RG), prostaglandins (PGF2), progestogens, gonadotropins (FSH and GSFA, HCG).

A known method of stimulation using PMSG for 8-9 days after calving at a dose of 7 ME per kg of body weight or at a dose of 4 ME per kg of body weight with the simultaneous administration of 2.0 cm3 of a 0.1% aqueous solution of carbo-choline (Gorev E.L. The use of hormonal drugs on industrial-type dairy farms // Dairy and beef cattle breeding. - 1980. - No. 6. - p. 32-33).

The method was used on animals with productivity from 2500 to 3000 kg of milk per lactation. At the same time, accelerated recovery of ovarian and uterine function was achieved during the first month after calving. However, with early insemination, embryonic mortality increased compared to animals without stimulation.

A known method of stimulating the recovery of the reproductive function of cows after childbirth using surfagon - a synthetic analogue of the releasing hormone. Surfagon is used from 8 to 15 days after delivery at a dose of 20 mcg once. At the same time, the terms of the first ovulation and involution of the uterus were reduced from 3 to 7 days, and the pregnancy of cows by the 60th day after calving increased, on average, by 12% (Chomaev AM, Sanusi M. The use of RN-RG for the regulation of sexual function in cows / WR Butler, Nutritional interactions with reproductive performance in dairy cattle // Anim. Reprod. Sc. - v.60-61 . - 2000. - pp.449-457).

However, hormonal therapy does not always have a positive effect, and can also lead to undesirable consequences, in particular, inflammation of the reproductive organs and infertility.

Positive results were also obtained on the use of Fosprenil (stimulant of natural resistance and immunity) and Gamavit (complex biotonic agent) on the livestock of several stud farms in Russia.

In the Lokot stud farm (Bryansk region), Gamavit was used during the breeding company and during the period of weaning foals. Mares with sexual cycle disorders (sluggish estrus, persistent corpus luteum, delayed maturation of follicles) and to increase the effectiveness of artificial insemination were given Gamavit twice with an interval of 7-10 days at doses from 12 to 25 ml per head, depending on body weight (approximately 20 ml per 600 kg live weight). At the same time, foaled mares received the first injection of the drug on the 3rd day after foaling, single mares - 21 days before the start of the breeding season, mares that did not foal after the third cycle - from 30 days after the last insemination or mating. (The use of phosprenyl and gamaumite to increase the yield and safety of foals. http://www.micro-plus.ru/: A.V. Deeva, M.L. Zaitseva, E.V. Kormanovskaya, E.O. Stikina, R .V. Belousova.).

However, the use of these drugs requires compliance with a rather complex regimen of drug administration, as well as the use of large doses of these drugs, which increases the cost of prevention and treatment.

The closest solution is a way to increase the fertility of farm animals using the drug Ovariovit. (Borodynya V.I. The incidence of sports mares with ovarian hypofunction and their treatment. // Agricultural sciences and agro-industrial complex at the turn of the century. - 2013., P. 121-125.).

However, the use of this drug gives a positive result only in complex therapy, does not increase the overall resistance of the animal, and also significantly increases the complexity and cost of treatment.

The objective of the invention is to expand the arsenal of ways to increase the fertility of mares using a preparation of natural origin containing an extract of antlers and Siberian larch.

The essence of the invention lies in the fact that in a method for increasing the fertility of mares, including the introduction of a drug based on an alcoholic extract of reindeer antlers and arabinogalactan, according to the invention, the drug is administered intramuscularly, 5 days before mating, three times, with an interval of one day, at a dose of 5 ml.

The implementation of the method.

Experiments to develop a method for increasing the fertility of mares were carried out under the production conditions of the Favorit peasant farm of the Republic of Kazakhstan and the Galiev V.P. peasant farm. Irkutsk region.

As a therapeutic agent to increase the fertility of mares, we took a drug that is an alcohol extract from reindeer antlers with arabinogalactan.

An alcoholic extract from reindeer antlers was prepared according to the method of V.G. Shelepova and N.S. Osintsev (P RU No. 2008906 dated March 13, 1994) by stepwise extraction of reindeer antlers. The resulting extract was filtered, separated from alcohol by evaporation on a water bath. After that, to the obtained extract (100 ml) was added 5-10 wt. % of arabinogalactan powder and placed on an electronic stirrer for 30 minutes (P RU No. 2691110 dated 06/11/2019).

The resulting mixture was diluted with sterile saline to the initial volume of the alcoholic extract, and the stabilizer nipagin (methyl ester of paraoxybenzoic acid) was added.

The resulting preparation in terms of biochemical and biological properties met the requirements as a tonic (biologically active additive). The immunostimulating effect of the drug is enhanced by a combination of reindeer antler extract and the use of arabino-galactan, a polysaccharide obtained from Siberian larch, which has a powerful immunomodulatory, antiseptic, probiotic effect on the animal's body.

1. Determination of the dose and frequency of administration of the drug

To select the dose and frequency of administration of the proposed drug of natural origin, 9 groups of horses (mares) were created with reproductive problems that arose mainly after the completion of a sports career, racing. Before experience, foals from 3 to 5 years old could not be obtained from these horses. Mares were impregnated by natural mating with proven stallions, from whom they received offspring every year.

The animals of the first three groups were given the drug at a dose of 3 ml five days before mating (3 injections), three days before (2 injections), a day before (1 injection), once every two days at a dose of 5 ml. Three more groups of horses were given the drug at a dose of 5 ml - five days before mating (3 injections), three days before 2 injections), the third per day (1 injection), once every two days at a dose of 5 ml. Mares of the last three groups - the drug was administered at a dose of 10 according to the same scheme as the previous groups - five days before mating (3 injections), three days before mating 2 injections), the third per day (1 injection), once every two days in dose of 5 ml. The readiness of horses for mating was checked by a stallion with a probe, as well as by rectal examination.

The results of studying the optimal dose of the drug and the frequency of its use are shown in table 1.

The use of the proposed drug in mares with fertilization problems in various doses and frequency of administration showed positive results. When using various schemes, the yield of foals was high - from 70% to 95%. According to the results shown in Table 1, it was noted that the highest (95%) result was obtained when using the drug at a dose of 5 ml, 5 days before mating, three times, with an interval of one day.

The decrease in effectiveness when using a higher dose of the drug - 10 ml, in the case of a single application, is due to insufficient intake of active substances. And with a threefold introduction, the decrease in fertility is explained by the inhibitory effect on the body of a high dose of the drug, which has a cumulative effect. A dose of 3 ml, according to the results of the study, was less effective than a dose of 5 ml.

2. Comparison of the activity of drugs to increase the fertility of mares.

For experience in comparing the effectiveness of the proposed drug with the approved drugs used in horse breeding, groups of horses with reproductive problems were created - mares of breeding value, most often, those who finished their sports career. Animals of the first group were injected with a preparation containing antlers, the second ovariovit, the third gamavit. The drug with antlers was administered 5 days before mating 3 times with an interval of 1 day. The rest of the drugs were administered according to the manufacturer's instructions. Gamavit was administered s.c. at a dose of 0.04 ml/kg of body weight a few hours before insemination. Ovariovit -1 time in 2-3 days, starting from the 1st day of estrus (2-4 injections). The readiness of horses for mating was checked by a stallion with a probe, as well as by rectal examination.

The results of the experiment on the comparative effectiveness of increasing the fertility of mares proposed by us and commercial preparations approved for this purpose are shown in Table 3.

In the group of horses that were injected with Gamavit at doses recommended by the manufacturer, they received the lowest percentage of fertilization and the yield of foals - only 2 heads per 10 mares. When using the drug Ovariovit in accordance with the scheme specified in the instructions, a higher effect was obtained - 5 foals from 10 mares. When using the proposed drug at a dose of 5 ml and three times with an interval of 1 day five days before mating, it was possible to obtain foals from all 10 mares. However, it should be taken into account that clinically healthy mares with exclusively problems with pregnancy were used in this experiment.

Thus, the conducted studies have shown that the test preparation containing antlers is more effective in increasing the fertility of mares and obtaining foals than the proposed approved preparations Gamavit and Ovariovit.

The conducted studies have shown the high efficiency of using a preparation of natural origin containing an extract of antlers and Siberian larch to increase the fertility of mares used in reproduction at the end of a sports career.

It has been established that intramuscular administration of the test drug to mares 5 days before mating three times with an interval of one day at a dose of 5 ml contributes to their fertilization.

Examples of the practical use of the drug in sports horse breeding.

Example 1

Mare Riga, age 18 years. From the age of 3 she was introduced into reproduction and foaled three times. Then she competed in triathlon for 5 years. At the end of her sports career, she had problems with fertilization for 4 years. Fertilization attempts did not result in pregnancy.

The drug was used 5 days before mating, three times, with an interval of one day at a dose of 5 ml. After using the drug, she successfully covered herself and endured the foal. Later fertilized and foaled without the drug

Example 2

Raymond's mare, 13 years old, was impregnated for the first time and gave birth to a foal at 2 years old. Then she participated in horse racing for 5 years. Fertilization attempts over the next five years did not lead to pregnancy. In this case, various stallions were used. After the start of the use of the proposed drug, according to the proposed scheme - 5 days before mating, three times, with an interval of one day at a dose of 5 ml, healthy offspring were obtained for two years. In the third year, pregnancy occurred without the use of this drug and also ended with the birth of a healthy foal.

Thus, the proposed method for increasing the fertility of mares using a drug based on an alcoholic extract of reindeer antlers and arabinogalactan, both in the experiment and under production conditions, makes it possible to increase the fertility of mares and the yield of foals, as well as to continue the use of horses after the end of their sports career.

Claims (1)

A method for increasing the fertility of mares, characterized by the introduction of a preparation based on an alcoholic extract of reindeer antlers and arabinogalactan intramuscularly 5 days before mating, three times, with an interval of one day, at a dose of 5 ml.