RU2615396C1 - Method for subclinical mastitis prevention for cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: cows are intracisternally given Orbenin DC once prior to drying off. On the second day after calving, cows intramuscularly receive additional Timogen 0.01% solution, dosed as 3 mg/kg once a day for 5 days.

EFFECT: application of the claimed method eliminates the development of clinical mastitis, reduces the risk of subclinical mastitis, which increases biosafety of milk preparation technology, ensures preservation of dairy productivity; reduces the number of somatic cells in milk, improving the milk quality, reduces treatment costs of mammary gland pathology.

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Description

The invention relates to veterinary pathology, namely the prevention and treatment of breast dysfunctions in female farm animals. The most effective way of conducting such prophylaxis is to prevent the occurrence of subclinical mastitis in cows.

A known method for the prevention of mastitis in cows, including the use of the drug Mamifort Sekado in the form of a suspension for intracisternal administration during the dry period (A. Arkhipov, Adequate treatment for acute mastitis is the key to the well-being of the herd / A.A. Arkhipov, A.T. Stolyar // Veterinary medicine. - 2008. - No. 11. - S. 15-17. - [1]).

The main disadvantage of such prevention is that the drug is contraindicated in animals with hypersensitivity to penicillin antibiotics.

Closest to the proposed method is a method for the prevention of mastitis in cows, comprising the use of a biologically active anti-inflammatory drug Orbenin DC, which has a wide spectrum of antibacterial activity, active against gram-positive bacteria, usually isolated from the secretion of the udder in the dry period. It is highly active against Streptococcus agalactiae, other types of streptococci, staphylococci (including strains resistant to penicillin) and corynebacteria (Corynebacterium pyogenes) (Modin A.N. Prevention of mastitis in cows in the dry period / A.N. Modin , N.T. Klimov, L.I. Efanova // Zootechny. - 2010. - No. 10. - S. 27-28. - Prototype. - [2]).

The main disadvantage of this method is that it does not have a very high prophylactic effectiveness and acts for a relatively short period of time, since it is applied intracisternally just before the start of the cows. At the same time, there remains a risk of a decrease in milk productivity of up to 25% and a decrease in the quality of milk.

The objective of the invention is to eliminate the above disadvantages in the prevention of subclinical mastitis in cows.

The problem is achieved due to the fact that in the known method for the prevention of subclinical mastitis in cows, which consists in a single intracisternal administration of Orbenin DC to cows before starting them, according to the invention, on the second day after calving, they are additionally intramuscularly injected with a 0.01% solution of Timogen in a dose 3 μg / kg of weight 1 time per day for 5 days.

Thymogen has a regulatory effect on the reactions of cellular and humoral immunity, as well as on nonspecific resistance of the body. The drug enhances the differentiation of lymphoid cells due to the expression of differentiation antigens on lymphocytes. Thymogen exhibits high affinity for thymocyte membrane receptors and induces the expression of CD2 + DR + molecules on lymphocyte membranes in vitro. Timogen increases the activity of 5'-ectonucleotidase and induces the expression of Thy-1 on thymocytes, which indicates its activating effect on the processes of lymphocyte differentiation in the thymus. The drug normalizes the ratio of T-helpers and T-suppressors in patients with immunodeficiency states. It is shown that thymogen also stimulates the colony-forming activity of bone marrow cells. The drug contributes to the normalization of body resistance indicators (Instructions for use of the drug Timogen (Thymogenum) 0.01% solution for injection in ampoules of 1 ml. Approved by the Pharmacological Committee M3 of the Russian Federation on 10.01.2001 - [3]).

The tests were carried out on 2 groups of cows with 90 animals each in OJSC OPK Krasnaya Zvezda, Oryol District, Oryol Region. It was necessary to find out the effectiveness of immunomodulators on the example of a 0.01% solution of the drug Timogen in the prevention of subclinical mastitis in cows, used in addition to the intracisternal administration of the drug Orbenin DC.

Before transferring to the dry period after the last milking, experimental group cows were once intracisternally injected with Orbenin DC once in each quarter of the udder, and on the second day after calving, they were intramuscularly injected with a 0.01% solution of Timogen in a dose of 3 μg / kg of weight 1 time per day within 5 days.

In cows of the control group, mastitis was prevented according to the known method using only the Orbenin DC preparation. After the last milking, these cows were injected once intracisternally into each quarter of the udder Orbenin DC before being transferred to the dry period.

Example 1

Before transferring to the dry period after the last milking, the cows of the control group were injected with a disposable plastic syringe, once intracisternally, into each quarter of the udder, Orbenin DC.

As a result of such prophylaxis, milk was sold in 2 grades or as non-sorted, depending on the number of somatic cells (from 580 to 950 thousand / cm ³ ). The average daily milk yield per cow was 14.5 liters of milk.

Example 2

Before transferring to the dry period after the last milking, experimental group cows were administered once intracisternally in each quarter of the udder with one disposable plastic syringe with Orbenin DC, and on the second day after calving, the cows were injected intramuscularly with a 0.01% Timogen solution at a dose of 3 μg / kg body weight Once a day for 5 days.

The additional use of thymogen allowed the farm to sell milk in the first grade with the content of somatic cells in the range of 200-350 thousand / cm ³ . The average daily milk yield per cow was 20.7 liters of milk.

The resulting milk in the prevention of subclinical mastitis in cows by the known method was sold in grade 2 or as non-sorted, while the prevention of breast pathologies by the proposed method allowed to improve the quality of milk (see table. 1).

The quality indicators of milk obtained as a result of the prevention of mastitis in animals of these groups are presented in table. one.

Using the proposed method for the prevention of subclinical mastitis in cows allows:

- to exclude the development of clinical mastitis, as well as reduce the risk of subclinical mastitis, which increases the biosecurity of production technology when receiving milk;

- maintain the milk production of cows;

- reduce the number of somatic cells in milk;

- improve the quality of milk;

- reduce the cost of treating cows with breast pathology. This significantly increases the effectiveness of ongoing prophylaxis of subclinical mastitis in cows.

The results of the prevention of mastitis in animals of these groups are presented in table. 2.

As you can see, the prophylactic effectiveness of the simultaneous use of a 0.01% solution of the drug Timogen with the drug Orbenin DC is significantly higher than in the prevention of mastitis in animals by a known method. So, the combined use of a 0.01% solution of the drug Timogen with the drug Orbenin DC can reduce the occurrence of subclinical mastitis in cows by 13.3% compared with the known method and prevent the development of clinical mastitis. It should also be noted that long-term results were observed after calving of cows, which made it possible to verify the high preventive effect of thymogen administration. In addition, there were no postpartum complications.

The proposed method for the prevention of subclinical mastitis in cows has been successfully applied at OAO OPK Krasnaya Zvezda, Oryol District, Oryol Region.

Claims (1)

A method for the prevention of subclinical mastitis in cows, which consists in a single intracisternal administration of Orbenin DC to cows before starting them, characterized in that on the second day after calving, they are additionally intramuscularly injected with a 0.01% solution of Timogen in a dose of 3 μg / kg weight 1 time per day for 5 days.