RU2524634C1 - Method of stimulation of poultry growth - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention is intended to stimulate the growth of poultry. The growth stimulators are used as amides of formula 1

of palmitic (n=14), stearic (n=16) acids and amide of oleic acid of formula 2

, which are used in the form of salts with pharmacologically acceptable acids.

EFFECT: method is safe, increases effectively the weight gain of chickens.

4 tbl, 1 ex

Description

The invention relates to the field of agriculture, and in particular to methods of stimulating poultry growth.

A known method of stimulating the growth of birds, including the use of vitamins, hormones, amino acids [1, 2].

A known method of stimulating the growth of birds, including the use of antibiotics, we have chosen as a prototype [3].

However, the use of antibiotics can be detrimental to saprophytic forms of microbes, which leads to vitamin deficiencies, allergic diseases, disorders of the gastrointestinal tract, etc.

The aim of the invention is to develop a new effective method of stimulating the growth of birds.

The goal is achieved in that as the active substance in the developed method of stimulating bird growth, amides of acids of the formula 1 (n = 14; 16) and 2 are used, which are used in the form of salts with pharmacologically acceptable acids (hydrochloric, sulfuric, phosphoric, maleic, amber and others):

The chemical name of compound 1 (n = 14) is palmitic acid (3-dimethylamino-propyl) amide.

The chemical name of compound 1 (n = 16): stearic acid (3-dimethylamino-propyl) amide.

The chemical name of compound 2 is oleic acid (3-dimethylamino-propyl) amide.

Amides 1 and 2 are obtained from the corresponding acids 4 (n = 14; 16), 5 and N, N-dimethyl-1,3-diamipropane 3 according to the scheme:

An example of obtaining palmitic acid amide 1 (n = 14):

A mixture of 25.64 g (0.1 mol) of palmitic acid 4 (n = 14) and 10.21 g (0.1 mol) of amine 3 is heated at 150-155 ° C for 4 hours with distillation of volatile products, then at 170-175 ° C for 30 minutes also with distillation of volatile products. It is cooled and the resulting product 1 (n = 14) is purified by column chromatography on alumina in chloroform. Yield 33.37 g (98%), mp. 59-61 ° C.

¹ H NMR spectrum, CDCl ₃ (δ, ppm): 0.854-0.898 (3H, triplet); 1.248-1.273 (24H, multiplet); 1.567-1.681 (4H, multiplet); 2.112-2.163 (2H, triplet); 2.225 (6H, singlet); 2.346-2.388 (2H, triplet); 3.304-3.363 (2H, quartet); 6.927 (1H, singlet). Found,%: C 74.40; H 12.70; N, 8.05. Calculated,%: C 74.05; H 13.02; N 8.22.

An example of obtaining stearic acid amide 1 (n = 16):

Synthesize analogously to the palmitic acid amide from 28.44 g (0.1 mol) of stearic acid 4 (n = 16) and 10.21 g (0.1 mol) of amine 3. Yield 35.01 g (95%), so pl. 63-64 ° C. ¹ H NMR spectrum, CDCl ₃ (δ, ppm): 0.854-0.898 (3H, triplet); 1.186-1.249 (28H, multiplet); 1.568-1.712 (4H, multiplet); 2.113-2.164 (2H, triplet); 2.225 (6H, singlet); 2.346-2.388 (2H, triplet); 3.304-3.363 (2H, quartet); 6.928 (1H, singlet). Found,%: C 74.60; H 13.20; N, 7.63.

Calculated,%: C 74.93; H 13.12; N, 7.59.

An example of obtaining oleic acid amide 2:

Synthesize similarly to palmitic acid amide from 28.24 g (0.1 mol) of oleic acid 5 and 10.21 g (0.1 mol) of amine 3. Yield 35.19 g (96%), light yellow oil. ¹ H NMR spectrum in CDCl ₃ (δ, ppm): 0.854 - 0.898 (3H, multiplet); 1.264-1.299 (20H, multiplet); 1.572-1.681 (4H, multiplet); 1.994 - 2.053 (4H, multiplet); 2.112-2.163 (2H, triplet); 2.225 (6H, singlet); 2.347-2.389 (2H, triplet); 3.304-3.363 (2H, quartet; 5.320-5.357 (2H, quartet); 6.928 (1H, singlet). Found,%: С 75.00; Н 12.20; N 7.20. Calculated,%: С 75.35; Н 12.64; N 7.64.

The obtained amides of acids 1 (n = 14; 16) and 2 are used as poultry growth stimulants. For this purpose, amide aqueous solutions are prepared. To dissolve amides in water, it is necessary to add the calculated amount (one equivalent) of pharmacologically acceptable mineral or organic acids (hydrochloric, sulfuric, phosphoric, succinic, citric and others), which play a secondary role, that is, contribute to the dissolution of amides in water, but do not affect poultry growth stimulation effect.

An example of poultry growth stimulation.

Apply 0.01% solutions of amides 1 (n = 14; 16) and 2 in terms of the free base. To prepare 10 liters of a working solution of 0.01% concentration (in terms of the free base), 1 g of the corresponding amide 1 (n = 14; 16) or 2 is weighed, mixed with 500 ml of water, one equivalent of acid is added, mixed at 40-50 ° C until a solution forms and the volume is adjusted to 10 liters. Tables 1, 2, and 3 show the amounts of acid for amide 1 (n = 14; 16) and 2.

Table 1 The amount of acid required to prepare 10 liters of a 0.01% solution of palmitic derivative 1 (n = 14) Salt Maleic Sulfuric Hydrogen bromide Sorbic 0.142 g in terms of HCl 0.226 g 0.191 g 0.3 15 g in terms of HBr 0.438 g table 2 The amount of acid required to prepare 10 liters of a 0.01% solution of stearic derivative 1 (n = 16) Salt Maleic Sulfuric Hydrogen bromide Sorbic 0.128 g in terms of HCl 0.203 g 0.172 g 0.284 g in terms of HBr 0.394 g

Table 3 The amount of acid required to prepare 10 liters of a 0.01% solution of oleic derivative 2 Salt Maleic Sulfuric Hydrogen bromide Sorbic 0.129 g in terms of HCl 0.204 g 0.173 g 0.286 g in terms of HBr 0.397 g

The resulting solution is evaporated to the bird (instead of water) for 18 days. Table 4 shows the test results of growth-promoting activity in chickens 2-4 days old (obtained by incubating eggs of outbred laying hens).

Table 4 The test results of the growth-promoting activity of fatty acid amides 1 (n = 14: 16) and 2 No. of groups of animals A drug The total mass of 10 chickens before the experiment, g The total mass of 10 chickens after 18 days of drinking, g Weight gain compared to control,% one Palmitic acid amide 1 (n = 14) as a salt with hydrochloric acid 394.6 1280.45 8.0 2 Palmitic acid amide 1 (n = 14) as a salt with sulfuric acid 384.0 1279.26 7.9 3 Palmitic acid amide 1 (n = 14) as a salt with sorbic acid 389.2 1281.63 8.1 four Stearic acid amide 1 (n = 16) as a salt with hydrochloric acid 387.5 1235.39 4.2 5 Oleic acid amide 2 in the form of a salt with hydrochloric acid 394.2 1273.33 7.4 6 Control group (received water) 397.8 1185.60 -

From the data of table 4 it is seen that the increase in body weight of the chickens of the experimental groups is 4.2 - 8.1%. According to the table (experimental groups 1-3), we can also conclude that the type of acid used does not affect the increase in body weight, that is, the growth-promoting effect is due exclusively to acid amides 1 (n = 14; 16) and 2.

"Acute" toxicity of substances.

It was established that the introduction into the stomach of laboratory rats of 5% aqueous solutions of 3 ml of once palmitic acid amide 1 (n = 14), stearic acid amide 1 (n = 16) and oleic acid amide 2 does not cause death of laboratory rats. Signs of an acute toxic reaction in rats were not established within 10 days of observation.

The technical result.

The technical result, objectively manifested in the implementation of the proposed method, is as follows:

1. Increase in weight gain of chickens up to 8%.

2. Lower costs when raising chickens.

3. Reducing labor costs in connection with the use of free drinking during the implementation of the claimed method.

4. Compatibility with current veterinary treatments for poultry rearing.

5. The substances proposed for use are biodegradable both in the body and in the environment to harmless fatty acids, as well as N, N-dimethyl-1,3-diaminopropane, which is easily oxidized to the simplest harmless compounds.

Literature

1. Veterinary drugs. Directory. Ed. HELL. Tretyakova. - M., Aropromizdat, 1988. - P.207-223.

2. Veterinary drugs. Directory. Ed. D.F. Osidze. - M., "Ear", 1981. - S. 410-414.

3. Brains I.E. Antibiotics in veterinary medicine. - M., "Kolos", 1971. - S. 75-83.

Claims (1)

A method of stimulating poultry growth, including the use of growth-promoting substances, characterized in that amides of the formula 1 palmitic (n = 14), stearic (n = 16) acids and oleic acid amide of the formula 2 are used as growth stimulants

which are used in the form of salts with pharmacologically acceptable acids.