RU2513559C1 - Method of producing biologically active pectin - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to production of polysaccharides from plant material. The method involves extracting the material with a threefold amount of 1% ammonium oxalate solution for 1.5 hours on a boiling water bath three times. The material used is oil cake obtained after separating flavonoids from common tansy flowers. The obtained extracts are mixed, filtered through several layers of gauze and pectins are precipitated in the filtrate with threefold excess 96% ethyl alcohol. The obtained precipitate is then washed successively with 96% ethyl alcohol, acetone and ether and dried over concentrated sulphurica acid.

EFFECT: invention enables to obtain biologically active pectin with anti-inflammatory action.

Description

The invention relates to medicine and relates to a method for producing a biologically active substance - pectin, which has anti-inflammatory activity, from the waste products of the drug “Tanacehol”.

The aim of the invention is to expand the resource base and increase the efficiency of production. This goal is achieved by extracting cheap raw materials - waste products of the Tanacekhol preparation containing flavonoids from the flowers of common tansy (Tanacetum vulgare L.) for the production of biologically active pectin, which has anti-inflammatory effect, has solubilizing activity and is not toxic.

The production method is as follows: 0.1 kg of meal obtained after separation of flavonoids from the flowers of common tansy, pour 0.3 l of a 1% solution of ammonium oxalate, extracted for 1.5 hours in a boiling water bath three times. The extracts obtained are combined, filtered through several layers of gauze and pectins are precipitated in the filtrate with a three-fold excess of 96% ethyl alcohol. The precipitate obtained is washed successively with 96% ethyl alcohol, acetone and ether, and dried over concentrated sulfuric acid.

The precipitate is an amorphous substance of light gray color. The total yield of pectin is 6.5%. Optically active. To determine the composition, the isolated pectin was subjected to acid hydrolysis. Chromatographic analysis of the hydrolyzate revealed the presence of glucose, rhamnose, xylose and arabinose.

Analysis of the conducted studies of biological activity showed that the substance isolated by the method described above exerts a pronounced inhibitory effect on the development of the exudative component of the inflammatory reaction initiated by formalin and the reduction of treatment time. So, already in the early stages of treatment of the inflammatory reaction, a significant reduction in edema was observed. Complete suppression of the inflammatory reaction and restoration of the initial limb volume of the rats during treatment with pectin was observed by the third day of the experiment. Under the influence of pectin in rats, the ESR value at 4 hours after drug administration was 1.6 ± 0.2, and the leukocyte level was 39.91% lower than in the control group. Normalization of ESR and leukocyte counts was observed on the 7th day of the experiment and amounted to 1.6 ± 0.4 and 11395.8 ± 216.8, respectively. A distinct advantage of the product obtained by the proposed method is noted in comparison with the known ones (calendula infusion and indomethacin).

When studying the acute toxicity of the substance obtained by the indicated method, it was impossible to determine LD ₅₀ due to its extremely low toxicity. The introduction of pectin at the maximum possible dose (1000 mg / kg orally), determined by solubility and the acceptable volume of administration, did not cause changes in the general condition of animals and the composition of peripheral blood. Pathomorphological studies did not reveal changes in internal organs in animals.

Animal studies were carried out in accordance with the Rules for the use of experimental animals (Order of the Ministry of Health of the USSR No. 75 of 08/12/1987) and the Federal Law of the Russian Federation "On the Protection of Animals from Cruelty" of 01/01/1997.

INFORMATION SOURCES

1. Aleeva G.N. The role of excipients in ensuring the pharmaceutical and therapeutic properties of drugs (review) / G.N. Aleeva, M.V. Zhuravleva, R.Kh. Khafizyanova // Chemical and Pharmaceutical Journal. - 2009. - No. 4. - S. 51-56

2. Mashkovsky M.D. Medicines / M.D. Mashkovsky. - 15th ed., Rev., Rev. and add. - M.: RIA "New Wave", 2007. - 1206 p.

3. Safonova M.Yu. Spectrophotometric method for determining the content of polysaccharides in thalli Cetrariaislandica (L.) Ach. / M.Yu.Safonova // Plant resources. - 1999. - T.35, issue 2. - S.101-105

4. Sychev I.A. Study of the anti-inflammatory effect of yellow melilot polysaccharides / I.A.Sychev, V.M.Smirnov, T.Yu. Kolosova // Russian Medical and Biological Bulletin named after Acad. I.P. Pavlova. - Ryazan, Russian State Medical University. - 2002. - No. 3-4. - S.71-76

5. Khotimchenko M.Yu. Sorption properties and pharmacological activity of non-starch polysaccharides: abstract. dis. Dr. med.science / M.Yu. Khotimchenko, GOU VPO "Vladivostok State Medical University of Roszdrav". -Vladivostok, 2011 .-- 47 p.

6. Chekulaev G.Yu. Organic chemistry. Teaching guidelines: Polysaccharides. Chemical structure and biological activity / G.Yu. Chekulaeva - Ryazan: Ryazan State Medical University, 2005. - 34 p.

7. Yakovlev G.P. Medicinal raw materials of plant and animal origin. Pharmacognosy: a training manual / G.P. Yakovlev. - St. Petersburg: SpetsLit, 2006 .-- 845 p.

8. Park W.H., Kim C.H., Lee Y.C., Kim C.H. Anti-inflammatory effects of a traditional Korean herbal formulation, Silsosangami, consisting of seven medical herbs: effect of hemolysis, neutrophil function, and gene expression of iNOS and COX-2. VasculPharmacol. 2004 Aug; 42 (1): 7-15.

Claims (1)

A method of producing pectin with biological activity, characterized in that for expanding the raw material base, meal was used as a meal after separation of flavonoids from the flowers of common tansy, extraction of which is carried out three times with a 1% solution of ammonium oxalate for 1.5 hours in boiling water bath three times; the obtained extracts are combined, filtered through several layers of gauze and pectins are precipitated in the filtrate with a three-fold excess of 96% ethyl alcohol; and the precipitate obtained is washed successively with 96% ethyl alcohol, acetone and ether, and dried over concentrated sulfuric acid.