RU2505298C1 - Method of treatment of acute poisoning of animals with neonicotinoid insecticides - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention is intended for treatment of acute poisoning of animals with neonicotinoid insecticides. The method comprises intravenous administration of diazepam, Ringer's solution and unitiol.

EFFECT: method improves effectively the survival of animals, reduces the concentration of neonicotinoid insecticides in the body.

3 tbl, 2 ex

Description

The invention relates to veterinary toxicology and is intended for the treatment of acute poisoning of animals with neonicotinoid insecticides.

A known method of treating poisoning with organophosphorus insecticides, including the administration of animals carboxyme and atropine in doses 2-3 times lower than when they are separately administered [Patent No. 2415668 C2, A61K 31/215, A61K 31/46, A61P 39/00].

The disadvantage of this method is the low efficiency in the treatment of acute poisoning of animals with neonicotinoid insecticides.

The closest in technical essence to the claimed method is a method for the treatment of acute nicotine poisoning, including intravenous administration of a 1% solution of novocaine of 20-50 ml, glucose solution of 5% 500 ml. Intramuscularly the introduction of a solution of magnesium sulfate 25% at a dose of 10 ml. With intravenous seizures, a 2.5% solution of diazepam in a dose of 5 ml, artificial respiration. With an intravenous heart rhythm disorder, a 10% solution of procainamide in a dose of 10 ml. When bradycardia subcutaneous 0.1% solution of atropine [Luzhnikov EA First aid for acute poisoning / E.A. Luzhnikov, Yu.N. Ostapenko, G.N. Sukhodolova. - M .: Publishing house BINOM, 2009. - 144 p.].

The disadvantage of this method of treatment is the low effectiveness of treatment in acute poisoning with neonicotinoid insecticides of animals.

The technical result of the invention is to increase the effectiveness of the treatment of animals in acute poisoning with neonicotinoid insecticides.

The technical result is achieved in that a method for the treatment of acute poisoning of animals with neonicotinoid insecticides involves the administration of diazepam intravenously, Ringer's solution and unitiol are additionally administered intravenously.

The use of Ringer's solution helps to eliminate toxic substances from the body, increases the effectiveness of treatment.

The use of unitiol helps to reduce the concentration of neonicotinoid insecticides in the organs and tissues of animals, increases the effectiveness of treatment.

The method is as follows: animals with symptoms of acute poisoning with neonicotinoid insecticides are injected intravenously with a 0.5% diazepam solution at a dose of 0.5 mg / kg body weight, then Ringer's solution is administered intravenously at a dose of 20 ml / kg body weight and 5% unitiol in dose of 25 mg / kg body weight.

Example 1. The effectiveness of treatment in acute poisoning with rat neonicotinoid insecticides.

Neonicotinoid insecticides: imidacloprid or thiacloprid was administered to groups of outbred white rats intragastrically in lethal doses.

Two groups of rats after intragastric administration of imidacloprid (n = 20) or thiacloprid (n = 20) in lethal doses were not treated.

Two control groups of rats 5 minutes after intragastric administration of imidacloprid (n = 20) or thiacloprid (n = 20) were injected intravenously with 0.5% diazepam solution at a dose of 0.5 mg / kg body weight.

Two experimental groups of rats 5 minutes after intragastric administration of imidacloprid (n = 20) or thiacloprid (n = 20) were injected intravenously with 0.5% diazepam solution at a dose of 0.5 mg / kg body weight, Ringer's solution was administered intravenously at a dose of 20 ml / kg body weight and 5% unitiol solution was administered intravenously at a dose of 25 mg / kg body weight.

Table 1 presents the results of the effectiveness of treatment of rats in acute poisoning with neonicotinoid insecticides: imidacloprid or thiacloprid.

Table 1 The effectiveness of treatment of rats in acute poisoning with neonicotinoid insecticides: imidacloprid or thiacloprid Name and dose of neonicotinoid insecticide, test result / Name and dose of the medicinal product, number of animals in the group Imidacloprid, 1000 mg / kg, single dose Thiacloprid once 300 mg / kg Fell Survived Fell Survived Without treatment, n = 20, n = 20 19 one twenty 0 Diazepam at a dose of 0.5 mg / kg body weight once, n = 20, n = 20 eleven 9 10 10 Ringer's solution at a dose of 20 ml / kg body weight, unitiol at a dose of 25 mg / kg body weight once, diazepam at a dose of 0.5 mg / kg body weight once, n = 20, n = 20 5 fifteen four 16 n is the number of animals in the group Note: the death of animals occurred during the first and second days after the introduction of lethal doses of neonicotinoid insecticides.

As can be seen from table 1, the treatment efficiency is higher in the experimental groups, where animals after acute poisoning with imidacloprid or thiacloprid were injected with diazepam at a dose of 0.5 mg / kg body weight, Ringer's solution at a dose of 20 ml / kg body weight, unitiol at a dose of 25 mg / kg body weight - 75% and 80% of surviving animals, respectively.

The method of high performance liquid chromatography was used to determine the residual amounts of imidacloprid and thiacloprid in the blood and liver of rats 4 hours after the administration of toxic doses of neonicotinoid insecticides and treatment. Table 2 presents the results of a study of rat blood and liver on the content of imidacloprid or thiacloprid.

table 2 The content of imidacloprid or thiacloprid in the blood and liver of rats in acute poisoning with neonicotinoid insecticides 4 hours after
toxic doses, M ± m Name and dose of neonicotinoid insecticide, content of neonicotinoid insecticide in the blood and liver of rats / Name and dose of the drug, number of animals in the group Imidacloprid, 300 mg / kg, single dose Thiacloprid, 300 mg / kg, single dose Blood mg / kg Liver, mg / kg Blood mg / kg Liver, mg / kg Without treatment, n = 3, n = 3 20.54 ± 1.59 26.04 ± 0.83 41.53 ± 1.55 136.48 ± 3.28 Diazepam at a dose of 0.5 mg / kg body weight once, n = 3, n = 3 13.57 ± 1.24 17.15 ± 1.25 25.28 ± 0.64 76.39 ± 2.39 Ringer's solution at a dose of 20 ml / kg body weight, unitiol at a dose of 25 mg / kg body weight once and diazepam at a dose of 0.5 mg / kg body weight once, n = 3, n = 3 5.75 ± 0.73 6.32 ± 1.14 12.36 ± 0.71 39.58 ± 1.04

The smallest content of imidacloprid and thiacloprid in blood samples and liver of rats was achieved after treatment with the proposed method: when diazepam was administered at a dose of 0.5 mg / kg body weight, Ringer's solution at a dose of 20 ml / kg body weight and unitiol at a dose of 25 mg / kg body weight. The concentration of imidacloprid after treatment by the proposed method decreased in blood samples by 3.6 times, in the liver - 4.1 times. The concentration of thiaclopride in blood and liver samples after treatment with the proposed method decreased by 3.4 times.

Example 2. The effectiveness of the treatment of acute poisoning with neonicotinoid insecticides in dogs.

Outbred dogs of three to four months of age were once orally administered imidacloprid or thiacloprid at a dose of 30 mg / kg body weight.

Dogs of the two treatment groups were not treated.

Dogs of the two control groups 90 minutes after the administration of toxic doses of neonicotinoid insecticides were injected intravenously with a 0.5% diazepam solution at a dose of 0.5 mg / kg of body weight.

Dogs of the two experimental groups 90 minutes after the administration of toxic doses of neonicotinoid insecticides were injected intravenously with a 0.5% diazepam solution at a dose of 0.5 mg / kg body weight, Ringer's solution at a dose of 20 ml / kg body weight, 5% unitiol solution at a dose of 25 mg / kg body weight.

The clinical status of the animals was evaluated, and the imidacloprid and thiacloprid levels in the blood of animals were determined by high performance liquid chromatography 90 minutes after the administration of toxic doses of neonicotinoid insecticides and 180 minutes after the administration of drugs.

Symptoms of acute poisoning, recorded 40.0 ± 8.4 minutes after the administration of neonicotinoid insecticides, were characterized by periodic tremor of certain muscle groups of the chest and pelvic limbs. Tremor in animals in groups without treatment lasted for a day.

After intravenous administration of diazepam at a dose of 0.5 mg / kg body weight, tremor occurred less frequently in animals with periods of 10-15 minutes for 4.5 ± 0.4 hours.

After intravenous administration of diazepam at a dose of 0.5 mg / kg body weight, Ringer's solution at a dose of 20 ml / kg body weight and unitiol at a dose of 25 mg / kg body weight in dogs, one-time vomiting and termination of tremor were noted for 1.6 ± 0 ,2 hours.

The residual content of imidacloprid or thiacloprid in the blood of dogs is presented in table 3.

Table 3 The content of imidacloprid or thiacloprid in the blood of dogs with oral administration of neonicotinoid insecticides, M ± m Name and dose of neonicotinoid insecticide, content of neonicotinoid insecticide in the blood of dogs, mg / kg / Name and dose of the drug, number of animals in the group Imidacloprid, 30 mg / kg, single dose Thiacloprid, 30 mg / kg, single dose Blood after 90 min, mg / kg Blood 180 min after drug administration, mg / kg Blood after 90 min, mg / kg Blood 180 min after drug administration, mg / kg Without treatment, n = 2, n = 2 15.05 ± 0.25 13.11 ± 0.15 20.12 ± 0.98 23.83 ± 3.37 Diazepam at a dose of 0.5 mg / kg body weight once, n = 6, n = 6 19.47 ± 3.35 6.75 ± 0.62 15.67 ± 0.89 10.47 ± 0.53 Ringer's solution at a dose of 20 ml / kg body weight, unitiol at a dose of 25 mg / kg body weight once, diazepam at a dose of 0.5 mg / kg body weight once, n = 6, n = 6 15.12 ± 0.88 3.36 ± 0.41 18.21 ± 1.35 3.71 ± 0.98

As can be seen from table 3, 90 minutes after the introduction of toxic doses of neonicotinoid insecticides, their content in the blood of dogs is on the same level. The greatest decrease in the concentration of imidacloprid and thiacloprid in the blood of dogs was noted 180 minutes after intravenous administration of diazepam at a dose of 0.5 mg / kg body weight, Ringer's solution at a dose of 20 ml / kg body weight and unitiol at a dose of 25 mg / kg body weight - by 74.4% and 84.4%, respectively. The severity of clinical symptoms of poisoning decreased with a decrease in residual amounts of neonicotinoid insecticides in the blood.

The proposed method for the treatment of acute poisoning of animals with neonicotinoid insecticides increases the survival of animals to 75-80% and reduces the concentration of neonicotinoid insecticides in the body by 74.6 ± 5.22%.

The proposed method for the treatment of acute animal poisoning with neonicotinoid insecticides has been tested in the laboratory conditions of the Department of Diagnostics, Internal Noncommunicable Diseases, Pharmacology, Surgery and Obstetrics of the Omsk State Agrarian University named after P. A. Stolypin.

Information sources

1. Patent No. 2415668, A61K 31/215, A61K 31/46, A61P 39/00.

2. Luzhnikov EA First aid for acute poisoning / E.A. Luzhnikov, Yu.N. Ostapenko, E.N. Sukhodolova. M .: Publishing house BINOM, 2009 .-- 144 p. (prototype).

Claims (1)

A method for the treatment of acute poisoning of animals with neonicotinoid insecticides, comprising the administration of diazepam intravenously, characterized in that Ringer's solution and unitiol are also administered intravenously.